Identification and use of molecules implicated in pain

ABSTRACT

The invention relates to the use of:  
     (a) an isolated gene sequence that is down-regulated in the spinal cord of a mammal in response to mechanistically distinct first and second models of neuropathic or central sensitization pain;  
     (b) an isolated gene sequence comprising a nucleic acid sequence of any of Tables I to VI;  
     (c) an isolated gene sequence having at least 80% sequence identity with a nucleic acid sequence of any of Tables I to VI;  
     (d) an isolated nucleic acid sequence that is hybridizable to any of the gene sequences according to (a), (b) or (c) under stringent hybridisation conditions;  
     (e) a recombinant vector comprising a gene sequence or nucleic acid sequence according to any one of (a) to (d);  
     (f) a host cell containing the vector according to (e);  
     (g) a non-human animal having in its genome an introduced gene sequence or nucleic acid sequence or a removed or down-regulated gene sequence or nucleic acid sequence according to any one of (a) to (d);  
     (h) an isolated polypeptide comprising an amino acid sequence at least 90% identical to an amino acid sequence encoded by a nucleotide sequence according to any one of (a) to (d), or a variant polypeptide thereof with sequential amino acid deletions from the C terminus and/or the N-terminus;  
     (i) an isolated polypeptide encoded by a nucleotide sequence according to any one of (a) to (d); or  
     (j) an isolated antibody that binds specifically to a polypeptide according to (h) or (i);  
     in the screening of compounds for the treatment of pain, or for the diagnosis of pain.  
     The invention also relates to the use of naturally occurring compounds such as peptide ligands of the expression products of the above gene sequences and their associated signal transduction pathways for use in the treatment of pain.

[0001] This application claims the priority of United Kingdomapplication NO. 0118354.0, filed Jul. 27, 2001, and United Kingdomapplication NO. 0202883.5, filed Feb. 7, 2002; the entire contents ofwhich applications are hereby incorporated by reference herein.

FIELD OF THE INVENTION

[0002] The present invention relates to nucleic acids, their expressionproducts and pathways involved in pain, and their use in screening formolecules that can alleviate pain. The invention further relates tomethods for the assay and diagnosis of pain in patients.

BACKGROUND TO THE INVENTION

[0003] Pain is currently classified into four general types.Post-operative acute pain can be successfully treated with existing painmedications of e.g. the opioid and non-steroidal anti-inflammatory(NSAID) types, and is usually short-term and self-limiting. A secondtype of pain, present e.g. in cancer and arthritis, is also responsiveto medication initially with a NSAID and in its later stages withopioids. Neuropathic pain arises from damage to the central orperipheral nerve systems, and is more effectively treated withantidepressants or anticonvulsants. A fourth type of pain called centralsensitization results from changes in the central nervous system as aresult of chronic pain, these changes often being irreversible anddifficult to treat. Nerve pain from shingles or diabetes falls into thisand the neuropathic category. Changes occur where pain is at firstpoorly controlled and gradually progress to the point where a person issensitive to stimuli which would not normally cause pain, for example alight touch. People with pain of this kind often describe a widening ofthe pain area to include areas which had originally not been injured orwhich were thought not to be involved in pain. This classification is,however based on clinical symptoms rather than on the underlying painmechanisms.

[0004] Opiates such as morphine belong to a traditional class ofpain-relieving compounds that are now recognized as binding to opiatereceptors. Naturally occurring polypeptides have also been found to haveopiate-like effects on the central nervous system, and these includeβ-endorphin, met-enkephalin and leu-enkephalin.

[0005] Salicin was isolated at the beginning of the 19th century, andfrom that discovery a number of NSAIDs such as aspirin, paracetamol,ibuprofen, flurbiprofen and naproxen were developed. NSAIDs are by farthe most widely used pain-relieving compounds, but can exhibit sideeffects, in particular irritation of the GI tract that can lead to theformation of ulcers, gastrointestinal bleeding and anemia.

[0006] Interest in the neurobiology of pain is developing: see acolloquium sponsored by the US National Academy of Sciences in December1998 concerning the neurobiology of pain and reviewed in The Scientist13[1], 12, 1999. Many pain mechanisms were discussed including the roleof the capsaicin receptors in pain, (M. J. Caterina et al., Nature, 389,816-824, 1997). Large dosages of capsaicin were reported to disable thatreceptor, (W. R. Robbins et al., Anesthesia and Analgesia, 86, 579-583,1998). Additionally, a tetrodotoxin-resistant sodium channel found insmall diameter pain-sensing neurons (PN3) was discussed (A. N. Akopianet al., Nature, 379, 257-262, 1986) and L. Sangameswaran et al., Journalof Biological Chemistry, 271, 953-956, 1996). Its involvement intransmission and sensitization to pain signals has been reported, (S. D.Novakovic et al., Journal of Neuroscience, 18, 2174-2187, 1998). Afurther tetrodotoxin-resistant sodium channel has been reported (S. Tateet al., Nature Neuroscience, 1, 653-655 1998).

[0007] Second messenger systems have also been shown to be importantsince knockout-mice lacking protein kinase C (PKC) γ were reported torespond to acute pain e.g. from a hot surface, but not to respond toneuropathic pain when their spinal nerves are injured (Malmberg et al.,Science, 278, 279-283 (1997).

[0008] Present methods for identifying novel compounds that relieve painof one or more of the types indicated above suffer from the defect thatthey are dependent either on the relatively limited number of receptorsknown to be involved in pain or on the empirical identification of newreceptors which is an uncertain process. In relation to known receptors,for example the opioid receptor, research directed to improved compoundsoffers the possibility of screenining compounds that have a bettertherapeutic ratio and fewer side effects. This does not lead naturallyto compounds for different pain receptors that have new modes of actionand new and qualitatively different benefits. Even when newly identifiedadditional receptors are taken into account, known receptors revolvearound tens of gene products. However, there are between 30,000 and40,000 genes in the genome of an animal and more of them are concernedwith nervous system function than with peripheral function. We thereforeconcluded that a large number of receptors and pathways are important tothe transduction of pain, but up to now have remained unknown.

SUMMARY OF THE INVENTION

[0009] It is an object of the invention to provide sequences of geneticmaterial for which no role in pain has previously been disclosed, andwhich are useful, for example, in:

[0010] identifying metabolic pathways for the transduction of pain

[0011] identifying from said metabolic pathways compounds having utilityin the diagnosis or treatment of pain

[0012] producing proteins and polypeptides with a role in thetransduction of pain;

[0013] producing genetically modified non-human animals that are usefulin the screening of compounds having utility in the treatment ordiagnosis of pain.

[0014] Identifying ligand molecules for receptors involved in saidmetabolic pathways and having utility in the treatment of pain.

[0015] It is yet a further object of the invention to provide researchtools, for example non-human animals and microorganisms, that can beused in screening compounds for pharmacological activity, especiallypain-reducing activity.

[0016] The present invention is based on sequences that aredown-regulated in two models of chronic pain, namelystreptozocin-induced diabetes and chronic constrictive injury (CCI) to anerve leading to the spine, for example the sciatic nerve.

[0017] In one aspect, the invention relates to the use in the screeningof compounds that are effective in the treatment of pain, or in thediagnosis of pain, of:

[0018] (a) an isolated gene sequence that is down regulated in thespinal cord of a mammal in response to first and second models of pain,for example in response to streptozocin-induced diabetes and in responseto a chronic constrictive injury to a nerve leading into the spine;

[0019] (b) an isolated gene sequence having at least 80% sequenceidentity with any of the nucleic acid sequences of Tables I-VI,preferably 85% sequence identity, more preferably 90%, increasinglypreferably 95%, most preferably 99%.

[0020] (c) an isolated nucleic acid sequence comprising a sequence thatis hybridizable to any of the gene sequences according to (a) or (b)under stringent hybridisation conditions;

[0021] (d) a recombinant vector comprising any of the gene sequencesaccording to (a) to (c);

[0022] (e) a host cell containing the vector according to (d);

[0023] (f) a non-human animal, for use in the screening of compoundsthat are effective in the treatment of pain, or in the diagnosis ofpain, having in its genome an introduced gene sequence or a removed ordown-regulated nucleotide sequence, said sequence becomingdown-regulated in the spinal cord of a mammal in response to first andsecond models of pain, particularly neuropathic or sensitisation pain,for example in response to streptozocin-induced diabetes and in responseto a chronic constrictive injury to a nerve leading into the spine;

[0024] (g) an isolated polypeptide containing an amino acid sequence atleast 90% identical to an amino acid sequence encoded by a nucleotidesequence according to any one of (a) to (d), or a variant thereof withsequential amino acid deletions from the C terminus and/or theN-terminus; or

[0025] (h) an isolated antibody that binds specifically to the isolatedpolypeptide according to (g).

[0026] The invention further provides a compound that is useful in thetreatment or diagnosis of pain and that modulates the action of anexpression product of a gene sequence that becomes down-regulated in thespinal cord of a mammal in response to first and second models of pain,for example being down-regulated both in response to streptozocininduced diabetes and in response to chronic constrictive injury to anerve leading into the spine.

[0027] The invention also relates to the use of naturally occurringcompounds such as peptide ligands of the expression products of theabove gene sequences and their associated signal transduction pathwaysfor the treatment of pain.

DESCRIPTION OF PREFERRED EMBODIMENTS

[0028] Definitions

[0029] Within the context of the present invention:

[0030] “Comprising” means consisting of or including. Thus nucleic acidcomprising a defined sequence includes nucleic acid that may contain afull-length gene or full-length cDNA. The gene may include any of thenaturally occurring regulatory sequence(s), such as a transcription andtranslation start site, a promoter, a TATA box in the case ofeukaryotes, and transcriptional and translational stop sites. Further,nucleic acid comprising a cDNA or gene may include any appropriateregulatory sequences for the efficient expression thereof in vitro.

[0031] “Isolated” requires that the material be removed from itsoriginal environment (e.g. the natural environment if it is naturallyoccurring). For example, a naturally occurring polynucleotide or apeptide present in a living animal is not isolated, but the samepolynucleotide or peptide, separated from some or all of the coexistingmaterials in the natural system, is isolated. Such polynucleotide can bepart of a vector and/or such polynucleotide or peptide can be part of acomposition, and still be isolated in that the vector or composition isnot a part of its natural environment.

[0032] “Mechanistically distinct” in relation to pain models impliesthat the pain is induced by mechanisms that differ in kind rather thanbeing variants of a similar pain model. Thus diabetic pain and chronicconstrictive pain models are mechanistically distinct, whereas spinalnerve ligation models and sciatic nerve ligation models which both workby ligation are not.

[0033] “Purified” does not require absolute purity; instead it isintended as a relative definition. Purification of starting materials ornatural materials from their native environment to at least one order ofmagnitude, preferably two or three orders, and more preferably four orfive orders of magnitude is expressly contemplated.

[0034] “Nucleic acid sequence” or “gene sequence” means a sequence ofnucleotides or any variant or homologue thereof, or truncated orextended sequence thereof, and is preferably indicated by a Genebankaccession number. Also within the scope of the present invention aredown-regulated nucleic acid sequences which encode expression productswhich are components of signaling pathways. This invention also includesany variant or homologue or truncated or extended sequence of thedown-regulated nucleotide sequence. Also within the scope of the presentinvention, the term “nucleic acid(s) product”, or “expression product”or “gene product” or a combination of these terms refers without beingbiased, to any, protein(s), polypeptide(s), peptide(s) or fragment(s)encoded by the down-regulated nucleotide sequence.

[0035] “Operably linked” refers to a linkage of polynucleotide elementsin a functional relationship. For instance, a promoter or an enhancer isoperably linked to a coding sequence if it regulates the transcriptionof the coding sequence. In particular, two DNA molecules (such as apolynucleotide containing a promoter region, and a polynucleotideencoding a desired polypeptide) are said to be “operably linked” if thenature of the linkage between the two polynucleotides does not (1)result in the introduction of a frame-shift mutation and (2) interferewith the ability of the polynucleotide containing the promoter to directthe transcription of the coding polynucleotide.

[0036] “Gene product” refers to polypeptide—which is interchangeablewith the term protein—which is encoded by a nucleotide sequence andincludes single-chain polypeptide molecules as well asmultiple-polypeptide complexes where individual constituent polypeptidesare linked by covalent or non-covalent means.

[0037] Polypeptides of the present invention may be produced bysynthetic means (e.g. as described by Geysen et al., 1996) or byrecombinant means.

[0038] The terms “variant”, “homologue”, “fragment”, “analogue” or“derivative” in relation to the amino acid sequence for the polypeptideof the present invention includes any substitution of, variation of,modification of, replacement of, deletion of or addition of one (ormore) amino acid from or to the sequence providing the resultantpolypeptide has the native gene product activity. In particular, theterm “homologue” covers homology with respect to structure and/orfunction. With respect to sequence homology, there is at least 90%, morepreferably at least 95% homology to an amino acid sequence encoded bythe relevant nucleotide sequence shown in Tables I-VI, preferably thereis at least 98% homology.

[0039] Typically, for the variant, homologue or fragment of the presentinvention, the types of amino acid substitutions that could be madeshould maintain the hydrophobicity/hydrophilicity of the amino acidsequence. Amino acid substitutions may include the use of non-naturallyoccurring amino acid analogues.

[0040] In addition, or in the alternative, the protein itself could beproduced using chemical methods to synthesize a polypeptide, in whole orin part. For example, peptides can be synthesized by solid phasetechniques, cleaved from the resin, and purified by preparative highperformance liquid chromatography (e.g. Creighton (1983) ProteinsStructures and Molecular Principles, W H Freeman and Co., New York,N.Y., USA). The composition of the synthetic peptides may be confirmedby amino acid analysis or sequencing (e.g. the Edman degradationprocedure).

[0041] Direct peptide synthesis can be performed using varioussolid-phase techniques (Roberge J Y et al Science Vol 269 1995 202-204)and automated synthesis may be achieved, for example, using the ABI 431A Peptide Synthesizer (Perkin Elmer) in accordance with the instructionsprovided by the manufacturer. Additionally, the amino acid sequence of agene product, or any part thereof, may be altered during directsynthesis and/or combined using chemical methods with a sequence fromother subunits, or any part thereof, to produce a variant polypeptide.

[0042] In another embodiment of the invention, a gene product natural,modified or recombinant amino acid sequence may be ligated to aheterologous sequence to encode a fusion protein. For example, forscreening of libraries for compounds and peptide agonists andantagonists of gene product activity, it may be useful to encode achimeric gene product expressing a heterologous epitope that isrecognised by a commercially available antibody. A fusion protein mayalso be engineered to contain a cleavage site located between a geneproduct sequence and the heterologous protein sequence, so that the geneproduct may be cleaved and purified away from the heterologous moiety.

[0043] The gene product may also be expressed as a recombinant proteinwith one or more additional polypeptide domains added to facilitateprotein purification. Such purification facilitating domains include,but are not limited to, metal chelating peptides such ashistidine-tryptophan modules that allow purification on immobilisedmetals (Porath J, Protein Expr Purif Vol 3 1992 p263-281), protein Adomains that allow purification on immobilised immunoglobulin, and thedomain utilised in the FLAGS extension/affinity purification system(Immunex Corp, Seattle, Wash., USA). The inclusion of a cleavable linkersequence such as Factor XA or enterokinase (Invitrogen, San Diego,Calif., USA) between the purification domain and the gene product isuseful to facilitate purification.

[0044] “Pain” includes chronic pain, neuropathic pain, pain arising fromcentral sensitisation, and in particular diabetic pain.

[0045] “Stringent hybridization conditions” is a recognized term in theart and for a given nucleic acid sequence refers to those conditionswhich permit hybridization of that sequence to its complementarysequence and closely homologous sequences. Conditions of high stringencymay be illustrated in relation to filter-bound DNA as for example 2×SCC,65° C. (where SSC=0.15M sodium chloride, 0.015M sodium citrate, pH 7.2),or as 0.5M NaHPO₄, 7% sodium dodecyl sulfate (SDS), 1 mM EDTA, at 65°C., and washing in 0.1×SCC/0.1% SDS at 68° C. (Ausubel F. M. et al.,eds, 1989, Current Protocols in Molecular Biology, Vol. I, GreenPublishing Associates, Inc., and John Wiley & Sons Inc., New York, at p.2. 10.3). Hybridization conditions can be rendered highly stringent byraising the temperature and/or by the addition of increasing amounts offormamide, to destabilize the hybrid duplex of non-homologous nucleicacid sequence relative to homologous and closely homologous nucleic acidsequences. Thus, particular hybridisation conditions can be readilymanipulated, and will generally be chosen depending on the desiredresults.

[0046] “Variants or homologues” include (a) sequence variations ofnaturally existing gene(s) resulting from polymorphism(s), mutation(s),or other alteration(s) as compared to the above identified sequences,and which do not deprive the encoded protein of function (b) recombinantDNA molecules, such as cDNA molecules encoding genes indicated by therelevant Genebank accession numbers and (c) any sequence that hybridizeswith the above nucleic acids under stringent conditions and encodes afunctional protein or fragment thereof.

[0047] Identified Sequences

[0048] The inventors have identified nucleotide sequences that give riseto expression products listed in Tables I-VI, that become differentiallyexpressed in the spinal cord in response to two distinct chronic painstimuli, for example neuropathic and/or central sensitization painstimuli, and that are believed to be involved in the transduction ofpain. In Tables I-VI, * denotes more preferred nucleic acid sequencesand ** denotes most preferred nucleic acid sequences. These nucleic acidsequences have not previously been implicated in the transduction ofpain.

[0049] The validity of the present experimental procedure was confirmedby the fact that nucleotide sequences were obtained as a result of theinvestigation whose function in the transduction of pain has beenpreviously confirmed and established. These nucleic acid sequences arenot part of this invention. Any of the nucleic acid sequences andexpression products can be used to develop screening technologies forthe identification of novel molecules for the prevention or treatment ofpain. These screening technologies could also be used to ascribe newpain therapeutic indications to molecules that have not previously beenidentified as being useful for the prevention or treatment of pain.Furthermore, the said nucleic acid sequences can be used as diagnostictools and for the development of diagnostic tools. TABLE I Sequenceswhose expression products are kinases Expression product Rat Mouse Humanor name Accession accession Accession (Reference) Number number NumberReaction Assay Pyruvate M24359 X97047 X56494 Tissue- Kinase kinase, M1(SEQ ID specific and M2 No's 1-3) promoter; subunit s Carbo- (Refs: 1-2)hydrate kinase

[0050] TABLE II Sequences whose expression products are receptorsExpression Product or Rat Mouse Human Name Accession accession Accession(Reference) Number Number Number Reaction Assay Dopamine I58000 DopamineReceptor receptor (SEQ ID receptor D.sub.1 ** NO 4) (Ref: 3) PutativeAF114168 GABA-B Receptor GABA-B1a (SEQ ID Receptor receptor ** No's 5-6)(Ref: 4)

[0051] TABLE III Sequences whose expression products are transportersExpression Rat Mouse Human Product or Accession Accession Accession NameNumber Number Number Reaction Assay Differentiation- AF271235 Trans-Transporter associated Na- (SEQ ID membrane dependent NO's 7-8)phosphate inorganic transport phosphate cotransporter (Ref: N/A)Putative vacuolar U87309 AAM47563.1 Vacuolar Transporter assemblyprotein (SEQ ID No assembly VSP41 gene 9) and traffic (Ref: N/A)

[0052] TABLE IV Sequences whose expression products are G-proteincoupled receptor proteins Expression Rat Mouse Human product orAccession Accession Accession Name Number Number Number Function AssayGit1 (G-protein- AF085693 Regulation of Ligand binding coupled receptoractivity of kinase-interactor (SEQ ID NO ARF6 in 1; GPCR kinase- 10-11)phosphatidyl- associated ADP- inisitol 3-kinase ribosylation signallingfactor) pathways; β2 (Ref: 5) adrenergic receptor regulation

[0053] TABLE V Sequences whose expression products are DNA-bindingproteins Expression Rat Mouse Human Product or Accession AccessionAccession Re- Name Number Number Number action Assay Putative X91866M11354 DNA DNA histone H3.3A (SEQ ID NO binding binding (Ref: 6) 12-13)

[0054] TABLE VI Sequences whose expression products are other enzymesExpression Rat Mouse Human product or Accession Accession Accession NameNumber Number Number Reaction Assay 3-Hydroxy 3- X52625 CholesterolLigase methylglutaryl biosynthesis coenzyme A (SEQ ID synthase, No's14-15) cytosolic * (Ref: 7) Acyl-CoA D360666 Fatty acid Ligasesynthetase II, (N/A) metabolism brain (Ref: N/A) Farnesyl M34477Isoprene Ligase diphosphate biosynthesis synthase ** (Seq ID No's (Ref:8) 16-17) Bendless protein AB032739 E12457 Protein Ligase (Ref: N/A)(Seq ID No's degradation 18-19) fatty acid X62888 Fatty acid Ligasesynthase (SEQ ID synthesis (Ref: 9) No's 20-21) Glutamine M91652 Aminoacid Ligase synthetase (EC (SEQ ID metabolism 6.3.1.2) ** NO's (Ref: 10)22-23) Putative seryl- X91257 Ligase Ligase tRNA synthetase (SEQ ID NO's(Ref No: 11) 24-25) Enolase, alpha X02610 X52379 M14328 Glycolysis Lyasealpha, non- (SEQ ID NO's neuronal (NNE) 26-27) (REF NO 12) Aldosereductase, X05884 Reduces Oxidoreductase lens (AREC (SEQ ID carbonyl11.1.21) NO's 28-29) (REF: 13) Cytochrome-c S79304 MitochrondrialOxidoreductase oxidase I, (SEQ ID energy mitochondrial NO's 30-31)metabloism (Ref: 14) Lactate U07181 X51905 Y00711 GlycolysisOxidoreductase dehydrogenase-B (SEQ ID (LDH-B) NO's 32-33) (Ref: 15)Putative x13923 Mitochrondrial Oxidoreductase cytochrome c energyoxidase VIB (EC (SEQ ID NO's metabolism 1.9.3.1) 34-35) (Ref: 16)Putative NADH: AF044953 Mitochrondrial Oxidoreductase ubiquinone (SEQ IDNo's energy oxidoreductasePG 36-37) metabolism IV subunit (Ref: 17)Putative succinate AF095938 AF171022 TCA cycle Oxidoreductasedehydrogenase (SEQ ID flavoprotein No's 38- (Ref: N/A) 39) PutativeJ04973 Mitochrondrial Oxidoreductase ubiquinol- (SEQ ID No's energycytochrome-c 40-41) metabolism reductase (EC 1.10.2.2) core protein II(REF: 18) Stearoyl-coA AB032243 M26270 Fatty acid Oxidoreductasedesaturase 2 * (SEQ ID biosynthesis (Ref: N/A) NO's 42-43) RibophorinI * X05300 Glycosolation Transferase (Ref: 19) (SEQ ID No's 44-45)Sulfotransferase- AF188699 Transferase Transferase like protein (SEQ ID(REF: 20) No's 46-47) ATP synthase, X56133 Mitochrondrial Hydrolase H+,alpha (SEQ ID energy subunit, No's 48-49) metabolism mitochondrial (EC3.6.1.34) (Ref: N/A) F1F0 ATPase U00926 Mitochrondrial Hydrolase deltasubunit (SEQ ID energy (REF: 21) No's 50-51) metabolism Putative D78013Pyrimidine Hydrolase dihydropyrimidin (SEQ ID NO's degradation aserelated protein 52-53) * (REF: 22) Heat shock 545392 M18186 M16660 Cellprotection Hydrolase protein 90 (SEQ ID (REF: 23) NO's 114- 115) Myelinbasic K00512 Myelin Ligand binding protein S (MBP SEQ ID No structuralS) (REF: 24) 54) protein Transferrin D38380 Iron transport Ligandbinding (Ref 25) (SEQ ID No's 55-56) Neurofilament, AF031880Cytoskeleton Ligand binding light molecular (SEQ ID weight (NF-L) No's57-58) (Ref: 26) Myelin- M16800 M31811 Cell adhesion Ligand bindingassociayted (SEQ ID molecule for glycoprotein NO's 59-60) postnatalneural (MAG) development (Ref: 27) NF-M middle M18628 CytoskeletonLigand binding molecular weight (SEQ ID neurofilament No's 61-62)protein (Ref: 28) Neuro- D32249 Protein sorting; Ligand bindingdegeneration (SEQ ID synaptic associated- No's 63-64) communicationprotein 1 and plasticity (Ref: 29) S-100 protein β- X01090 Zinc andLigand binding subunit (SEQ ID No calcium binding (REF: 30) 65)Microtubule- X60370 L06237 Cytoskeleton Ligand binding associatedprotein (SEQ ID protein, 1b (Map 1b) No's 66-67) neuronal (Ref: 31)growth/ regeneration; microtubule binding protein Putative cdc 37 D26564Cell signalling; Ligand binding homolog (Seq ID No's cell cycle (Ref:32) 68-69) protein Putative ras- U11293 Small GTP Ligand binding relatedprotein (SEQ ID No's binding protein Rab-5c 70-71) (Ref: 33) Putativegelsolin J04953 Cytoskeleton Ligand binding (Ref: 34) (SEQ ID proteinNo's 72- 73) Cd81 antigen U19894 X59047 M33680 Regulator for Ligandbinding (target of (SEQ ID neuron-induced antiproliferative No's 74-75)astrocyte antibody 1) differentiation (Ref: 35) microglial effectorfunctions Mobp81 (Myelin- X87900 Myelin Ligand binding associated/Oligo-(SEQ ID compaction dendrocytic basic N0's 76-77) protein 81) (Ref: 36)Syntaxin binding BC002869 Neuro- Ligand binding protein n-sec1, (Seq IDNo's transmission sec1 homolog 78-79) (Ref: N/A) Alpha-internexin M73049Cytoskeleton Ligand binding (Ref: 37) (SEQ ID protein; No's 80-81)neuronal intermediate filament that can self- assemble Putative β-AB024921 Cytoskeleton Ligand binding sarcoglycan A3b (SEQ ID protein(Ref: N/a) No's 82- 83) CGI-78 protein AF151835 AF151835 Unknown Ligandbinding Ref: 38) (SEQ ID No's 84-85) KIAA0143 D63477 TransmembraneLigand binding (Ref: 39) (SEQ ID NO's protein 86-87) Septin 2 D50918GTPase; Ligand binding (KIAA0128; (SEQ ID No's cytokinesis Ref 39)88-89) Nucleobindin Z36277 Unknown Ligand binding (Ref: 40) (SEQ ID No's90-91) Myelin protein M69139 S55427 Myelin Ligand binding SR13 (SEQ IDstructural (Ref: 41) NO's 92-93) protein B-Actin, V01217 X03672Structural Ligand binding cytoplasmic (SEQ ID protein (Ref: 42) NO's94-95) Ly6/neurotoxin AF141377 Neuro- Ligand binding (Lynx1) homolog(SEQ ID transmitter (Ref: 43) No's 96- 97) modulator Astrocytic AJ243949X86694 PKC substrate Ligand binding phosphoprotein, (SEQ ID PFA 15 geneNO's 98- (Ref: N/A) 99) PLIC-1 AF177345 Cytoskeleton Ligand binding(Ref: N/A) (SEQ ID interaction NO 100- 101) Nfx1 (tip AF093139 AF093140Unknown Logand binding associating (SEQ ID protein (TAP) NO's 102- gene)(Ref: N/A) 103) Alpha-Crystallin U04320 M73741 M28638 Stress Ligandbinding B (Ref N/A) SEQ ID response; heat NO's 104- shock element 105)Heat shock-like X70065 U73744 Y00371 Stress response Ligand bindingprotein 70 kD (SEQ ID (Ref: 44) No's 106- 107) Tau microtubule- X79321Microtubule Ligand binding associated protein (SEQ ID associated (Ref:45) NO's 108- protein 109) expressed in neurons Myelin, K03242 MyelinLigand binding Schwann cell, (SEQ ID structural Peripheral (P-0) No's110- protein (Ref: 46) 111) B-Tubulin class 1 AB011679 X04663 AF14139Structural Ligand binding (Ref: 47) (SEQ ID protein NO's 112- 113)Putative AF116910 RNA hydrolysis Hydrolase ribonuclease III (SEQ ID NO's(Ref: N/A) 116-117)

[0055] Production of Polypeptides Nucleic Acids

[0056] Vectors

[0057] Recombinant expression vectors comprising a nucleic acid can beemployed to express any of the nucleic acid sequences of the invention.The expression products derived from such vector constructs can be usedto develop screening technologies for the identification of moleculesthat can be used to prevent or treat pain, and in the development ofdiagnostic tool for the identification and characterization of pain. Theexpression vectors may also be used for constructing transgenicnon-human animals.

[0058] Gene expression requires that appropriate signals be provided inthe vectors, said signals including various regulatory elements such asenhancers/promoters from viral and/or mammalian sources that driveexpression of the genes or nucleic acid sequences of interest in hostcells. The regulatory sequences of the expression vectors used in theinvention are operably linked to the nucleic acid sequence encoding thepain-associated protein of interest or a peptide fragment thereof.

[0059] Generally, recombinant expression vectors include origins ofreplication, selectable markers, and a promoter derived from a highlyexpressed gene to direct transcription of a downstream nucleoidesequence. The heterologous nucleotide sequence is assembled in anappropriate frame with the translation, initiation and terminationsequences, and if applicable a leader sequence to direct the expressionproduct into the periplasmic space, the extra-cellular medium or cellmembrane.

[0060] In a specific embodiment wherein the vector is adapted forexpressing desired sequences in mammalian host cells, preferred vectorswill comprise an origin of replication from the desired host, a suitablepromoter and an enhancer, and also any necessary ribosome binding sites,polyadenylation site, transcriptional termination sequences, andoptionally 5′-flanking non-transcribed sequences. DNA sequences derivedfrom the SV40 or CMV viral genomes, for example SV40 or CMV origin,early promoters, enhancers, and polyadenylation sites may be used toprovide the required non-transcribed genetic elements.

[0061] A recombinant expression vector used in the inventionadvantageously also comprises an untranscribed polynucleotide regionlocated at the 3′end of the coding sequence (ORF), this 3′-UTR(untranslated region) polynucleotide being useful for stabilizing thecorresponding mRNA or for increasing the expression rate of the vectorinsert if this 3′-UTR harbours regulation signal elements such asenhancer sequences.

[0062] Suitable promoter regions used in the expression vectors arechosen taking into account the host cell in which the nucleic acidsequence is to be expressed. A suitable promoter may be heterologouswith respect to the nucleic acid sequence for which it controls theexpression, or alternatively can be endogenous to the nativepolynucleotide containing the coding sequence to be expressed.Additionally, the promoter is generally heterologous with respect to therecombinant vector sequences within which the construct promoter/codingsequence has been inserted. Preferred promoters are the LacI, LacZ, T3or T7 bacteriophage RNA polymerase promoters, the lambda PR, PL and Trppromoters (see EP-0 036 776), the polyhedrin promoter, or the p10protein promoter from baculovirus (kit Novagen; Smith et al., (1983);O'Reilly et al. (1992)).

[0063] Preferred selectable marker genes contained in the expressionrecombinant vectors used in the invention for selection of transformedhost cells are preferably dehydrofolate reductase or neomycin resistancefor eukaryotic cell culture, TRP1 for S. cerevisiae or tetracycline,rifampicin or ampicillin resistance in E. coli, or Levamsaccharase forMycobacteria, this latter marker being a negative selection marker.

[0064] Preferred bacterial vectors are listed hereafter as illustrativebut not limitative examples: pQE70, pQE60, pQE-9 (Quiagen), pD10,phagescript, psiX174, p.Bluescript SK, pNH8A, pNH16A, pNH18A, pNH46A(Stratagene); pKK223-3, pKK233-3, pDR540, pRIT5 (Pharmacia); pWLNEO,pSV2CAT, pOG44, pXT1, pSG (Stratagene); pSVK3, pBPV, pMSG, pSVL(Pharmacia); pQE-30 (QIA express).

[0065] Preferred bacteriophage recombinant vectors of the invention areP1 bacteriophage vectors such as described by Sternberg N. L.(1992;1994).

[0066] A suitable vector for the expression of any of the painassociated polypeptides used in the invention or fragments thereof, is abaculovirus vector that can be propagated in insect cells and in insectcell-lines. A specific suitable host vector system is the pVL 1392/1393baculovirus transfer vector (Pharmingen) that is used to transfect theSF9 cell line (ATCC N^(o) CRL 1711) that is derived from Spodopterafrugiperda.

[0067] The recombinant expression vectors of in the invention may alsobe derived from an adenovirus. Suitable adenoviruses are described byFeldman and Steig (1996) or Ohno et al. (1994). Another preferredrecombinant adenovirus is the human adenovirus type two or five (Ad 2 orAd 5) or an adenovirus of animal origin (Patent Application WO 94/26914)

[0068] Particularly preferred retrovirus for the preparation orconstruction of retroviral in vitro or in vivo gene delivery vehiclesinclude retroviruses selected from the group consisting of Mink-CellFocus Inducing Virus, Murine Sarcoma Virus, and Ross Sarcoma Virus.Other preferred retroviral vectors are those described in Roth et al.(1996), in PCT Application WO 93/25234, in PCT Application WO 94/06920,and also in Roux et al. (1989), Julan et al.(1992) and Nada et al.(1991).

[0069] Yet, another viral vector system that is contemplated is theAdeno Associated Viruses (AAV) such as those described by Flotte et al.(1992), Samulski et al. (1989) and McLaughlin et al. (1996).

[0070] Host Cells Expressing Pain Associated Polypeptides

[0071] Host cells that endogenously express pain associated polypeptidesor have been transformed or transfected with one of the nucleic acidssequences described herein, or with one of the recombinant vectordescribed above, particularly a recombinant expression vector, can beused in the present invention. Also included are host cells that aretransformed (prokaryotic cells) or are transfected (eukaryotic cells)with a recombinant vector such as one of those described above.

[0072] Preferred host cells used as recipients for the expressionvectors used in the invention are the following:

[0073] (a) prokaryotic host cells: Escherichia coli, strains. (i.e.DH5-α, strain) Bacillus subtilis, Salmonella typhimurium and strainsfrom species like Pseudomonas, Streptomyces and Staphylococcus for theexpression of up and down-regulated nucleic acid sequence modulated bypain, characterized by having at least 80% sequence identity with any ofthe nucleic acid sequences of Tables I-VI. Plasmid propagation in thesehost cells can provide plasmids for transfecting other cells.

[0074] (b) eukaryotic host cells: HeLa cells (ATCC N^(o) CCL2; N^(o)CCL2.1; N^(o) CCL2.2), Cv 1 cells (ATCC N^(o) CCL70), COS cells (ATCCN^(o) CRL 1650; N^(o) CRL 1651), Sf-9 cells (ATCC N^(o) CRL 1711), C127cells (ATCC N^(o) CRL-1804), 3T3 cells (ATCC N^(o) CRL-6361), CHO cells(ATCC N^(o) CCL-61), human kidney 293 cells (ATCC N^(o) 45504; N^(o)CRL-1573), BHK (ECACC N^(o)84100 501; N^(o)84111301), PC12 (ATCC N^(o)CRL-1721), NT2, SHSY5Y (ATCC N^(o) CRL-2266), NG108 (ECACCN^(o)88112302) and F11, SK-N-SH (ATCC N^(o) CRL-HTB-11), SK-N-BE(2)(ATCC N^(o) CRL-2271), IMR-32 (ATCC N^(o) CCL-127). A preferred systemto which the nucleic acids of the invention can be expressed areneuronal cell lines such as PC12, NT2, SHSY5Y, NG108 and F11, SK-N-SH,SK-N-BE(2), IMR-32 cell lines, COS cells, 3T3 cells, HeLa cells, 292cells and CHO cells. The above cell lines could be used for theexpression of any of the nucleic acid sequences of Tables I-VI.

[0075] When a nucleic acid sequence of any of Tables I-X is expressedusing a neuronal cell line, the sequence can be expressed through anendogenous promoter or native neuronal promoter, or an exogenouspromoter. Suitable exogenous promoters include SV40 and CMV andeukaryotic promoters such as the tetracycline promoter. The preferredpromoter when pain associated molecules are endogenously expressed is anendogenous promoter. A preferred promoter in a recombinant cell line isthe CMV promoter.

[0076] In a specific embodiment of the host cells described above, thesehost cells have also been transfected or transformed with apolynucleotide or a recombinant vector for the expression of a naturalligand of any of the nucleic acid sequences of any of Tables I-VI or amodulator of these expression products.

[0077] Proteins, Polypeptides and Fragments

[0078] The expression products of the nucleic acid sequences of TablesI-VI or fragment(s) thereof can be prepared using recombinanttechnology, from cell lines or by chemical synthesis. Recombinantmethods, chemical methods or chemical synthetic methods can be used tomodify a gene in order to introduce into the gene product, or a fragmentof the gene product, features such as recognition tags, cleavage sitesor other modifications. For efficient polypeptide production, theendogenous expression system or recombinant expression system shouldallow the expression products to be expressed in a manner that willallow the production of a functional protein or fragment thereof whichcan be purified. Preferred cell lines are those that allow high levelsof expression of polypeptide or fragments thereof. Such cell linesinclude cell lines which naturally express the nucleic acid sequence ofTables I-VI or common mammalian cell lines such as CHO cells or COScells, etc, or more specific neuronal cell lines such as PC12. However,other cell types that are commonly used for recombinant proteinproduction such as insect cells, amphibian cells such as oocytes, yeastand prokaryotic cell lines such as E. coli can also be used.

[0079] The expression products of Tables I-VI or fragments thereof canbe utilized in screens to identify potential therapeutic ligands, eitheras a purified protein, as a protein chimera such as those produced inphage display, as a cell membrane (lipid or detergent) preparation, orin intact cells.

[0080] The invention also relates generally to the use of proteins,peptides and peptide fragments for the development of screeningtechnologies for the identification of molecules for the prevention ortreatment of pain, and the development of diagnostic tools for theidentification and characterization of pain. These peptides includeexpression products of the nucleic acid sequences of Tables I-VI andpurified or isolated polypeptides or fragments thereof having at least90%, preferably 95%, more preferably 98% and most preferably 99%sequence identity with the any of the expression products of nucleicacid sequences of Tables I-VI. Expressed peptides and fragments of anyof these nucleic acid sequences can be used to develop screeningtechnologies for the identification of novel molecules for theprevention or treatment of pain. These screening technologies could alsobe used to ascribe new pain therapeutic indications to molecules, whichhave not previously been ascribed for the prevention or treatment ofpain. Furthermore the said expressed peptides and fragments can be usedas diagnostic tools and for the development of diagnostic tools.

[0081] Screening Methods

[0082] As discussed above, we have identified nucleic acid sequenceswhose expression is regulated by pain, particularly chronic pain andmore particularly diabetic pain. The expression products of thesenucleic acids can be used for screening ligand molecules for theirability to prevent or treat pain, and particularly, but not exclusively,chronic pain. The main types of screens that can be used are describedbelow. The test compound can be a peptide, protein or chemical entity,either alone or in combination(s), or in a mixture with any substance.The test compound may even represent a library of compounds.

[0083] The expression products of any of the nucleic acid sequences ofTables I-VI or fragments thereof can be utilized in a ligand bindingscreen format, a functional screen format or invivo format. Examples ofscreening formats are provided.

[0084] A) Ligand Binding Screen

[0085] In ligand binding screening a test compound with is contactedwith an expression product of one of the sequences of Tables I-VI, andthe ability of said test compound to interact with said expressionproduct is determined, e.g. the ability of the test compound(s) to bindto the expression product is determined. The expression product can be apart of an intact cell, lipidic preparation or a purifiedpolypeptide(s), optionally attached to a support, such as beads, acolumn or a plate etc.

[0086] Binding of the test compound is preferably performed in thepresence of a ligand to allow an assessment of the binding activity ofeach test compound. The ligand may be contacted with the expressionproduct either before, simultaneously or after the test compound. Theligand should be detectable and/or quantifiable. To achieve this, theligand can be labelled in a number of ways, for example with achromophore, radioactive, fluorescent, phosphorescent, enzymatic orantibody label. Methods of labelling are known to those in the art. Ifthe ligand is not directly detectable it should be amenable to detectionand quantification by secondary detection, which may employ the abovetechnologies. Alternatively the expression product or fragment thereofcan be detectable or quantifiable. This can be achieved in a similarmanner to that described above.

[0087] Binding of the test compound modifies the interaction of theligand with its binding site and changes the affinity or binding of theligand for/to its binding site. The difference between the observedamount of ligand bound relative to the theoretical maximum amount ofligand bound (or to the ligand bound in the absence of a test compoundunder the same conditions) is a reflection of the binding ability (andoptionally the amount and/or affinity) of a test compound to bind theexpression product.

[0088] Alternatively, the amount of test compound bound to theexpression product can be determined by a combination of chromatographyand spectroscopy. This can be achieved with technologies such as Biacore(Amersham Pharmacia). The amount of test compound bound to theexpression product can also be determined by direct measurement of thechange in mass upon compound or ligand binding to the expressionproduct. Alternatively, the expression product, compound or ligand canbe fluorescently labelled and the association of expression product withthe test compound can be followed by changes in Fluorescence EnergyTransfer (FRET).

[0089] The invention therefore includes a method of screening for painalleviating compounds, comprising:

[0090] a) contacting a test compound or test compounds in the presenceof a ligand with an expression product of any of the nucleic acidsequences of Tables I-VI or with cell expressing at least one copy ofthe expression product or with a lipidic matrix containing at least onecopy of the expression product;

[0091] b) determining the binding of the test compound to the expressionproduct, and

[0092] c) selecting test compounds on the basis of their bindingabilities.

[0093] In the above method, the ligand may be added prior to,simultaneously with or after contact of the test compound with theexpression product. Non limiting examples and methodology can be gainedfrom the teachings of the Molecular Probes handbook and referencestherein (Molecular Probes, Inc., 4849 Pitchford Ave, Eugene, USA),Methods in neurotransmitter receptor analysis (Yamamura H I., Enna, SJ., and Kuhar, M J., Raven Press New York, the Glaxo Pocket Guide toPharmacology, Dr. Michael Sheehan, Glaxo Group Research Ltd, Ware, HertsSG12 0DP), Bylund D B and Murrin L C (2000, Life Sciences, 67 (24)2897-911), Owicki J C (2000, J. biomol Screen (5) 297-306), Alberts etal (1994, Molecular Biology of the Cell, 3^(rd) Edn, Garland PublicationInc), Butler J E, (2000 Methods 22(1):4-23, Sanberg S A (2000, Curr OpinBiotechnol 11(1) 47-53), and Christopoulos A (1999, biochem Pharmacol58(5) 735-48).

[0094] B) Functional Screening

[0095] (a) Kinase Assays

[0096] The expression product of any of the nucleic acid sequences ofTables I-VI which encode a kinase, and in particular the nucleic acidsequence listed in Table I, is amenable to screening using kinase assaytechnology.

[0097] Kinases have the ability to add phosphate molecules to specificresidues in ligands such as binding peptides in the presence of asubstrate such as adenosine triphosphate (ATP). Formation of a complexbetween the kinase, the ligand and substrate results in the transfer ofa phosphate group from the substrate to the ligand. Compounds thatmodulate the activity of the kinase can be determined with a kinasefunctional screen. Functional screening for modulators of kinaseactivity therefore involves contacting one or more a test compounds withan expression product of one of the nucleic acid sequences of TablesI-VI which encodes a kinase, and determining the ability of said testcompound to modulate the transfer of a phosphate group from thesubstrate to the ligand.

[0098] The expression product can be part of an intact cell or of alipidic preparation or it can be a purified polypeptide(s), optionallyattached to a support, for example beads, a column, or a plate. Bindingis preferably performed in the presence of ligand and substrate to allowan assessment of the binding activity of each test compound.

[0099] The ligand should contain a specific kinase recognition sequenceand it should not be phosphorylated at its phosphoryation site. Theligand and/or substrate may be contacted with the kinase either before,simultaneously or after the test compound. Optionally the substrate maybe labelled with a kinase transferable labelled phosphate. The assaybeing monitored by the phosphorylation state of the substrate and/or theligand. The ligand should be such that its phosphorylation state can bedetermined. An alternative method to do this is to label the ligand witha phosphorylation-state-sensitive molecule. To achieve this, the ligandcan be labelled in a number of ways, for example with a chromophore,radioactive, fluorescent, phosphorescent, enzymatic or antibody label.If the ligand is not directly detectable it should be amenable todetection and quantification by secondary detection, which may employthe above technologies. Such technologies are known to those in the art.

[0100] Binding of the test compound to the kinase modifies its abilityto transfer a phosphate group from the substrate to the ligand. Thedifference between the observed amount of phosphate transfer relative tothe theoretical maximum amount of phosphate transfer is a reflection ofthe modulatory effect of the test compound. Alternatively, the degree ofphosphate transfer can be determined by a combination of chromatographyand spectroscopy. The extent of phosphorylation of the ligand peptide ordephosphorylation of the substrate can also be determined by directmeasurement. This can be achieved with technologies such as Biacore(Amersham Pharmacia).

[0101] The invention also provides a method for screening compounds forthe ability to relieve pain, which comprises:

[0102] (a) contacting one or more test compounds in the presence ofligand and substrate with an expression product of any of the sequencesof Tables I-VI which is a kinase or with a cell containing at least 1copy of the expression product or with a lipidic matrix containing atleast 1 copy of an expression product;

[0103] (b) determining the amount of phosphate transfer from thesubstrate to the ligand; and

[0104] (c) selecting test compounds on the basis of their capacity tomodulate phosphate transfer.

[0105] Optionally ligand, substrate and/or other essential molecules maybe added prior to contacting the test compound with expression productof step (a) or after step (a). Non limiting examples and methodology canbe gained from the teachings of the Molecular Probes handbook andreferences therein (Molecular Probes, Inc., 4849 Pitchford Ave, Eugene,USA), Methods in Molecular Biology 2000; 99: 191-201, Oncogene 2000 20;19(49): 5690-701, and FASAB Journal, (10, 6, P55, P1458, 1996, Pocius DAmrein K et al).

[0106] b) Receptor Assays

[0107] An expression product of any of the nucleic acid sequences ofTables I-VI which encodes a receptor, and in particular any of thenucleic acid sequences listed in Table II, is amenable to screeningusing receptor assay technology.

[0108] Receptors are membrane associated proteins that initiateintracellular signalling upon ligand binding. Therefore, theidentification of molecules for the prevention and treatment of pain canbe achieved with the use of a ligand binding assay, as outlined above.Such an assay would utilize an endogenous or non-endogenous ligand as acomponent of the ligand binding assay. The binding of this ligand to thereceptor in the presence of one or more test compounds would be measuredas described above. Such is the nature of receptors that the assay isusually, but not exclusively performed with a receptor as an intact cellor membraneous preparation.

[0109] The invention therefore includes a method of screening for painalleviating compounds, comprising:

[0110] a) contacting a test compound or test compounds in the presenceof a ligand with cell expressing at least one copy of the expressionproduct of any of the sequences of Tables I-VI which is a receptor orwith a lipidic matrix containing at least one copy of the expressionproduct;

[0111] b) determining the binding of the test compound to the expressionproduct, and

[0112] c) selecting test compounds on the basis of their bindingabilities.

[0113] c) Transporter Protein Assays

[0114] An expression product of any of the nucleic acid sequences ofTables I-VI which encodes a transporter protein, and in particular anyof the nucleic acid sequences listed in Table III, is amenable toscreening using transporter protein assay technology. Non limitingexamples of technologies and methodologies are given by Carroll F I, etal (1995, Medical Research Review, Sep15 (5) p419-444), Veldhuis J D andJohnson M l (1994, Neurosci. Biobehav Rep., winter 18(4) 605-12),Hediger M A and Nussberger S (1995, Expt Nephrol, July-Aug 3(4)p211-218, Endou H and Kanai Y, (1999, Nippon Yakurigaku Zasshi, Oct. 114Suppl 1:1p-16p), Olivier B et al (2000, Prog. Drug Res., 54, 59-119),Braun A et al (2000, Eur J Pharm Sci, oct 11, Suppl 2 S51-60) andMolecular Probes handbook and references therein (Molecular Probes,Inc., 4849 Pitchford Ave, Eugene, USA).

[0115] The main function of transporter proteins is to facilitate themovement of molecules across a cellular membrane. Compounds thatmodulate the activity of transporter proteins can be determined with atransporter protein functional screen. Functional screening formodulators of transporter proteins comprises contacting at least onetest compound with an expression product as aforesaid which is atransporter protein and determining the ability of said test compound tomodulate the activity of said transporter protein. The expressionproduct can be part of an intact cell, or lipidic preparation,optionally attached to a support, for example beads, a column or aplate. Binding is preferably performed in the presence of the moleculeto be transported, which should only able to pass through a cellmembrane or lipidic matrix with the aid of the transporter protein. Themolecule to be transported should be able to be followed when it movesinto a cell or through a lipidic matrix. Preferably the molecule to betransported is labelled to aid in characterization, e.g. with achromophore, radioactive, fluorescent, phosphorescent, enzymatic orantibody label. If the molecule to be transported is not directlydetectable it should be amenable to detection and quantification bysecondary detection, which may employ the above technologies. Themolecule to be transported may be contacted with the transporter proteinbefore, simultaneously with or after the test compound. If binding ofthe test compound to the transporter protein modifies its ability totransport molecules through a membraneous or lipidic matrix, then thedifference between the observed amount of transported molecule in acell/or through a lipidic matrix relative to the theoretical maximumamount is a reflection of the modulatory effect of the test compound.

[0116] The invention further provides a method for screening compoundsfor their ability to relieve pain, comprising

[0117] a) contacting at least one test compound in the presence oftransporter molecules with a cell containing at least one copy of anexpression product of any of the sequences of Tables I-VI which is atransporter protein or with a lipidic matrix containing at least onecopy of the expression product;

[0118] c) measuring the movement of transported molecules into or fromthe cell, or across the lipidic matrix; and

[0119] d) selecting test compounds on the basis of their ability tomodulate the movement of transported molecules.

[0120] d) G-Protein Coupled Receptor Protein Assays

[0121] An expression product of any of the nucleic acid sequences ofTables I-VI that encodes a G-protein coupled receptor protein, and inparticular any of the nucleic acid sequences listed in Table IV, isamenable to screening using G-protein coupled receptor protein assaytechnology.

[0122] G-protein coupled receptor proteins (GPCRs) are membraneassociated proteins whose main function is to transduce a signal througha cellular membrane. Upon ligand binding, GPCRs undergo a conformationalchange that allows complexing of the GPCRs with a G-protein. G-proteinspossess a GTP/GDP binding site. The formation of the G-protein/ligandcomplex allows exchange of GTP for GDP, resulting in a conformationalchange of the G-protein. This conformational change initiates signaltransduction.

[0123] Functional screening for modulators of GPCRs comprises contactingat least one test compound with an expression product as aforesaid whichis a G-protein coupled receptor protein, and assessing the ability ofthe test compound(s) to modulate the exchange of GTP for GDP or themodulation of the GPCR signal transduction pathway. The expressionproduct can be part of an intact cell or lipidic preparation, optionallyattached to a support, for example beads, a column or a plate. Bindingis preferably performed in the presence of a ligand, G-protein andGTP/GDP to allow an assessment of the binding activity of each testcompound. Alternatively components of the signaling pathway are alsoincluded. In particular, in a preferred embodiment, a labeled GTP isused and the ability of the test compound(s) to modulate the exchange ofGTP to GDP is determined. A further optional characteristic of the assaycan be the inclusion of a reporter molecule that enables monitoring theregulation of the signaling pathway. The ligand may be contacted withthe GPCR before, simultaneously with, or after the test compound.Binding of the test compound to the GPCR modifies its ability tomodulate the exchange of GTP for GDP and hence the modulation of signaltransduction. The difference between the observed amount of GTPexchanged for GDP relative to the theoretical maximum amount of GTP is areflection of the modulatory effect of the test compound. Likewise therelative activities of signal transduction reporter molecules are also areflection of the modulatory effect of the test compound. Non limitingexamples of technologies and methodologies can be found in MolecularProbes handbook and references therein (Molecular Probes, Inc., 4849Pitchford Ave, Eugene, USA), Glaxo Pocket Guide to Pharmacology,(Michael Sheehan, Pharmacology Division staff, Glaxo Group ResearchLtd., Ware, Herts SC12 0DP) and Xing et al (2000, J. Recept. Signal.Transduct. Res. 20(40 189-210).

[0124] The invention provides a method of screening compounds for theirability to relieve pain, comprising:

[0125] a) contacting at least one test compound in the presence of aligand, GTP/GDP, G-protein with a cell containing at least one copy ofan expression product of a sequence of Tables I-VI as aforesaid which isa G-protein coupled receptor protein or with a lipidic matrix containingat least one copy of the expression product;

[0126] b) measuring the exchange of GTP for GDP, and

[0127] c) selecting test compounds on the basis of their ability tomodulate said exchange.

[0128] In the above method, the ligand, GTP/GDP, G-protein and otheressential molecules can be added before, simultaneously with or afterthe contacting of the test compound(s) with the cell line or lipidicmatrix in step (a).

[0129] e) DNA-Binding Protein Assays

[0130] An expression product of any of the nucleic acid sequences ofTables I-VI that encodes a DNA-binding protein, and in particular any ofthe nucleic acid sequences listed in Table V, is amenable to screeningusing DNA-binding protein assay technology.

[0131] DNA binding proteins are proteins that are able to complex withDNA. The complexing of the DNA binding protein with the DNA in someinstances requires a specific nucleic acid sequence. Screens can bedeveloped in a similar manner to ligand binding screens as previouslyindicated and will utilise DNA as the ligand. DNA-binding protein assayscan be carried using similar principles described in ligand bindingassays as described above. Non limiting examples of methodology andtechnology can be found in the teachings of Haukanes B I and Kvam C(Biotechnology, 1993 Jan 11 60-63), Alberts B et al (Molecular Biologyof the Cell, 1994, 3^(rd) Edn., Garland Publications Inc, Kirigiti P andMachida C A (2000 Methods Mol Biol, 126, 431-51) and Molecular Probeshandbook and references therein (Molecular Probes, Inc., 4849 PitchfordAve., Eugene, USA).

[0132] The invention therefore includes a method of screening for painalleviating compounds, comprising:

[0133] a) contacting a test compound or test compounds in the presenceof a plurality of nucleic acid sequences with an expression product ofany of the nucleic acid sequences of Tables I-VI which is a DNA bindingprotein or with cells expressing at least one copy of the expressionproduct or with a lipidic matrix containing at least one copy of theexpression product;

[0134] b) determining the binding of the test compound to the expressionproduct, and

[0135] c) selecting test compounds on the basis of their bindingabilities.

[0136] In the above method, the plurality of nucleic acid sequence maybe added prior to, simultaneously with or after contact of the testcompound with the expression product.

[0137] f) Assays Using other Enzymes

[0138] Expression products of any of the nucleic acid sequences ofTables I-VI that encode other enzymes, e.g. ligases, lyases,oxidoreductases, transferases and hydrolases, and in particular any ofthe nucleic acid sequences listed in Table VI, is amenable to screeningusing appropriate assay technology.

[0139] Each class of enzyme has a defined function. Ligases have theproperty of being able to splice molecules together. This is achievedwith the conversion of ATP substrate to AMP. Therefore, the activity ofa ligase can be followed by monitoring the conversion of ATP to AMP.Such technologies are known to those in the art. Non limiting examplesand methodologies are illustrated by Ghee. T Tan et al (1996,Biochem J.314, 993-1000, Yang S W et al (1992, 15: 89(6) 2227-31 and refrencestherein, and in Molecular Probes handbook and references therein(Molecular Probes, Inc., 4849 Pitchford Ave, Eugene, USA).

[0140] The invention also provides a methods of screening for painalleviating compounds, comprising;

[0141] a) contacting one or more test compounds in the presence of ATPwith an expression product of any of the sequences of Tables I-VI whichis a ligase or with a cell expressing at least 1 copy of a expressionproduct or with a lipidic matrix containing at least 1 copy of anexpression product which is a ligase;

[0142] b) determining the amount of ATP converted to AMP, and

[0143] c) selecting test compounds on the basis of their ability tomodulate said conversion.

[0144] Lyases are enzymes which catalyse the cleavage of by reactionsother than hydrolysis. These enzymes can be grouped into seven groupsaccording to type of bond cleaved. These groups are carbon-carbon lyases(E.C. No 4.1), carbon-oxygene lyases (E.C. No 4.2), carbon-nitrogenlyases (E.C. No 4.3), carbon-sulphur lyase (E.C. No 4.4) carbon-halidelyases (E.C. No 4.5), phosphorous-oxygene lyases (E.C. No 4.6) and otherlyases (E.C. No 4.99) (Analytical Biochemistry 3^(rd) Edn, David J.Holme and Hazel Peck, Longman press). The enzyme commission number(E.C.) of the International Union of Biochemistry relates to the type ofreaction catalysed by the enzyme. Further teachings on how to developassays and screens for lyases can be obtained from Methods in Enzymology(Academic Press).

[0145] Oxidoreductases are enzymes that catalyse the transfer ofhydrogen or oxygen atoms or electrons. These enzymes can by sub-groupedinto twenty categories according to their specific mode of action. Thesegroups are oxidoreductases acting on the CH—OH group of donors (E.C.No1.1), oxidoreductases acting on the aldehyde or oxo group of donors(E.C. No 1.2), oxidoreductases acting on the CH—CH group of the donor(E.C. No 1.3), oxidoreductases acting on the CH—NH2 group of donors(E.C. 1.4), oxidoreductases acting on the CH—NH group of donor (E.C.1.5), oxidoreductases acting on the NADH or NADPH (E.C. No 1.6),oxidoreductases acting on other nitrogen compounds as donors (E.C. No1.7), oxidoreductases acting on a sulphur group of donors (E.C. No 1.8),oxidoreductases acting on a haem group of donors (E.C. No1.9),oxidoreductases acting on diphenols and related substances as donors(E.C. 1.10), oxidoreductases acting on hydrogen peroxide as acceptor(E.C. No 1.11), oxidoreductases acting on hydrogen as donor (E.C. No1.12), oxidoreductases acting on single doners with incorporation ofmolecular oxygen (E.C. No 1.13), oxidoreductases acting on paired donorswith incorporation of molecular oxygen (E.C. No 1.14), oxidoreductasesacting on superoxide radicals as acceptors (E.C. 1.15), oxidoreductasesoxidizing metal ions (E.C. No 1.16), oxidoreductases acting on —CH2groups (E.C. No 1.17), oxidoreductases acting on reduced ferredoxin asdonor (E.C. No 1.18), oxidoreductases acting on reduced flavodoxin asdonor (E.C. No 1.19) and other oxidoreductases (E.C. No 1.97)(Analytical Biochemistry 3^(rd) Edn, David J. Holme and Hazel Peck,Longman Press). The enzyme commission number (E.C.) of the InternationalUnion of Biochemistry relates to the type of reaction catalysed by theenzyme. Further teachings on how to develop assays and screens foroxidoreductases can be obtained from Methods in Enzymology (AcademicPress) with special reference to volume 249.

[0146] Transferases are enzymes that catalyse the transfer of specificgroups. They are classified into eight sub groups according to function,transferring one-carbon group (E.C. No 2.1), Transfering aldehyde orketonic residues (E.C. No 2.2), Acetyltransferases (E.C. 2.3),glycosyltransferases (E.C. No 2.4), transferring alkyl or aryl groupsother than methyl groups (E.C. No 2.5), transferring nitrogeneous groups(E.C. No 2.6), transferring phosphorous-containing groups (E.C. No 2.7)and transferring sulphur-containing groups (E.C. No 2.8) (AnalyticalBiochemistry 3^(rd) Edn, David J. Holme and Hazel Peck, Longman Press).The enzyme commission number (E.C.) of the International Union ofBiochemistry relates to the type of reaction catalysed by the enzyme.Further teachings on how to develop assays and screens for transferasescan be obtained from Methods in Enzymology (Academic Press).

[0147] Hydrolases are enzymes that catalyse hydrolytic reactions and aresub-grouped into eleven classes according to the type of reaction theycarry out. Hydrolases acting on ester bonds (E.C. No 3.1), hydrolasesacting on glycosyl compounds (E.C. No 3.2), hydrolases acting on etherbonds (E.C. No 3.3), hydrolases acting on peptide bonds (E.C. No 3.4),hydrolases acting on carbon-nitrogen bonds, other than peptide bonds(E.C. No 3.5), hydrolases acting on acid anhydrides (E.C. No 3.6),hydrolases acting on acid anhydrides (E.C. No 3.6), hydrolases acting oncarbon-carbon bonds (E.C. No 3.7), hydrolases acting on halide bonds(E.C. No 3.8), hydrolases acting on phosphorous-nitrogen bonds (E.C. No3.9), hydrolases acting on sulphur-nitrogen bonds (E.C. No 3.10) andhydrolases acting on carbon-phosphorous bonds (Analytical Biochemistry3^(rd) Edn, David J. Holme and Hazel Peck, Longman Press). The enzymecommission number (E.C.) of the International Union of Biochemistryrelates to the type of reaction catalysed by the enzyme. Furtherteachings on how to develop assays and screens for hydrolases can beobtained from Methods in Enzymology (Academic Press) with specialreference to volume 249.

[0148] C) In Vivo Functional Screen

[0149] Any of the nucleotide sequences described in Tables I-VI orhomologues thereof may be inserted by means of an appropriate vectorinto the genome of a lower vertebrate or of an invertebrate animal ormay be inactivated or down regulated in the genome of said animal. Theresulting genetically modified animal may be used for screeningcompounds for effectiveness in the regulation of pain. The invertebratemay, for example, be a nematode e.g. Caenorhabditis elegans, which is afavourable organism for the study of response to noxious stimuli. Itsgenome sequence has been determined, see Science, 282, 2012 (1998), itcan be bred and handled with the speed of a micro-organism (it is aself-fertilizing hermaphrodite) and can therefore be used in a highthroughput screening format (WO 00/63424, WO 00/63425, WO 00/63426 andWO 00/63427), and it offers a full set of organ systems, including asimple nervous system and contains many similarly functioning genes andsignaling pathways to mammals. A thermal avoidance model based on areflexive withdrawal reaction to an acute heat stimulus has beendescribed by Wittenburg et al, Proc. Natl. Acad. Sci. USA, 96,10477-10482 (1999), and allows the screening of compounds for thetreatment of pain with the modulation of pain sensation as an endpoint.

[0150] The genome of C. elegans can be manipulated using homologousrecombination technology which allows direct replacement of nucleicacids encoding C. elegans with their identified mammalian counterpart.Replacement of these nucleic acids with those nucleic acids outlinedabove would allow for the direct screening of test compound(s) withtheir expression products. Any of the pain-related genes described abovemay be ligated into a plasmid and introduced into oocytes of the worm bymicroinjection to produce germline transformants. Successful plasmidinjection into C. elegans and expression of inserted sequences has beenreported by Devgen B. V., Ghent, Belgium. It is also possible to produceby routine methods worms in which the target sequences aredown-regulated or not expressed (knock-out worms). Further non limitingexamples of methodology and technology can be found in the teachings ofHazendonk et al (1997, Nat genet. 17(1) 119-21), Alberts et al, (1994,Molecular Biology of the Cell 3^(rd) Ed. Garland Publishing Inc,Caenorhabditis elegans is anatomically and genetically simple),Broverman S et al, (1993, PNAS USA 15;90(10) 4359-63) and Mello et al(1991, 10(12)3959-70).

[0151] A further method for screening compounds for ability to modifyresponse to pain, e.g. relieve pain, comprises:

[0152] (a) contacting one or more test compounds with at least one Celegans containing at least one copy of a sequence as set out above;

[0153] (b) subjecting the C. elegans to a nociceptive stimulus;

[0154] (c) observing the response of the C. elegans to said stimulus;and

[0155] d) selecting test compounds on the basis of their ability tomodify the response of C. elegans to said stimulus.

[0156] Diagnostic Tools and Kits

[0157] Affinity Peptides, Ligands and Substrates

[0158] Pain associated polypeptides and fragments thereof can bedetected at the tissue and cellular levels with the use of affinitypeptides, ligands and substrates, which will enable a skilled person todefine more precisely a patient's ailment and help in the prescriptionof a medicament. Such affinity peptides are characterized in thatfirstly they are able to bind specifically to a pain associatedpolypeptide, and secondly that they are capable of being detected. Suchpeptides can take the form of a peptide or polypeptide for example anantibody domain or fragment, or a peptide/polypeptide ligand orsubstrate, or a polypeptide complex such as an antibody.

[0159] The preparation of such peptides and polypeptides are known tothose in the art. Antibodies, these may be polyclonal or monoclonal, andinclude antibodies derived from immunized animals or from hybridomas, orderivatives thereof such as humanized antibodies, Fab or F(ab′)2antibody fragments or any other antibody fragment retaining the antigenbinding specificity.

[0160] Antibodies directed against pain-associated gene productmolecules may be produced according to conventional techniques,including the immunization of a suitable mammal with the peptides orpolypeptides or fragment thereof. Polyclonal antibodies can be obtaineddirectly from the serum of immunized animals. Monoclonal antibodies areusually produced from hybridomas, resulting from a fusion betweensplenocytes of immunized animals and an immortalized cell line (such asa myeloma). Fragments of said antibodies can be produced by proteasecleavage, according to known techniques. Single chain antibodies can beprepared according to the techniques described in U.S. Pat. No.4,946,778. Detection of these affinity peptides could be achieved bylabeling. technologies which allow detection of peptides, such asenzymatic labeling, fluorescence labeling or radio-labeling are wellknown to those in the art. Optionally these affinity peptides, ligandsand substrates could themselves be detected with the use of a moleculethat has specific affinity to the peptides, ligands and substrates andis itself labeled.

[0161] The invention further provides a kit comprising;

[0162] (a) affinity peptide and/or ligand and/or substrate for anexpression product of a gene sequence that is down-regulated in thespinal cord of a mammal in response to first and second models ofneuropathic or central sensitization pain; and

[0163] (b) a defined quantity of an expression product of a genesequence that is down-regulated in the spinal cord of a mammal both inresponse to first and second models of neuropathic or centralsensitization pain,

[0164] for simultaneous, separate or sequential use in detecting and/orquantifying an expression product of a gene sequence that isdown-regulated in the spinal cord of a mammal in response to first andsecond models of neuropathic or central sensitization pain.

[0165] Complimentary Nucleic Acids

[0166] Pain associated nucleic acid sequences can be characterized atthe tissue and cellular levels with the use of complimentary nucleicacid sequences. Detection of the level of expression of pain associatednucleic acid sequences can help in the prognosis of a pain condition andthe prescription of a medicament. These complimentary nucleic acids arecharacterized in that they can hybridize to a pain associated nucleicacid sequence and their presence can be detected through varioustechniques. Such techniques are known to those in the art and mayinclude detection by polymerase chain reaction or detection by labelingof complimentary nucleic acid sequences by enzymatic labeling, affinitylabeling fluorescent labeling or radio labeling. Complimentary strandnucleic acid sequences of this invention are 10 to 50 bases long, morepreferably 15 to 50 bases long and most preferably 15 to 30 bases long,and hybridize to the coding sequence of the nucleic acid sequence.

[0167] A further aspect of this invention is a kit that comprises:

[0168] (a) nucleic acid sequences capable of hybridization to a nucleicacid sequence that is down-regulated in the spinal cord of a mammal inresponse to first and second models of neuropathic or centralsensitization pain; and

[0169] (b) a defined quantity of one or more nucleic acid sequencescapable of hybridization to a nucleic acid sequence that isdown-regulated in the spinal cord of a mammal in response to first andsecond models of neuropathic or central sensitization pain, forsimultaneous, separate or sequential use in detecting and/or quantifyinga gene sequence that is down-regulated in the spinal cord of a mammal inresponse to first and second models of neuropathic or centralsensitization pain.

[0170] Identification and Validation

[0171] Subtractive hybridization enables the identification of nucleicacid sequences whose expression profiles are modified by a stimulus.Upon stimulation of a system (in the case of this invention anociceptive stimulus on an animal model) all observed changes in thelevel of nucleic acid sequence expression are due to the reaction of thesystem to the stimulus. Characterization of these changes in expressionby way of identification of nucleic acid sequence and level ofexpression is both identification and validation.

[0172] The inventors have developed a four step process which allows forthe simultaneous identification and validation of nucleic acid sequenceswhose expression are regulated by a pain stimulus, preferably a chronicpain stimulus, and more preferably a diabetic pain stimulus. Thisprocess may comprise the following steps:

[0173] (a) induction of a nociceptive stimulus in test animals;

[0174] (b) extraction of nucleic acids from specific neuronal tissue oftest and control animals;

[0175] (c) selective amplification of differentially expressed nucleicacid sequences; and

[0176] (d) identification and characterization of differentiallyexpressed gene products that are modulated by a nociceptive stimulus.

[0177] The above process is described in more detail below.

[0178] (a) Induction of Nociceptive Stimulus

[0179] The effect of the selected nociceptive stimulus on the testanimal needs to be confirmable. The test subjects are therefore aspecies that has a “developed” nervous system, preferably similar tothat of humans, most preferably rats or mice. Advantageously, thenociceptive stimulus is analogous to known pain paradigms in humans. Onesuch paradigm of pain is the pain associated with diabetes, which can beinduced in rodents with the use of streptozotocin (STZ). The presentapplication requires the sequences to be down-regulated in two painmodels which may be, but are not limited to models of neuropathic painand/or central sensitization, and in which diabetic pain provides thefirst model and mechanical damage e.g. to a nerve leading into the spinecan provide an appropriate second model.

[0180] Streptozotocin (STZ) induces hyperglycemia and Type 1 diabetesmellitus in rats. In particular, STZ contains a glucose analogue thatallows it to be taken up by the glucose transporter 2 present on thesurface of pancreatic β cells, the site of insulin synthesis. Onceinside the cell, STZ causes a reduction in the level of nicotinamideadenine dinucleotide (NAD⁺). The decrease in NAD⁺ levels eventuallyleads to necrosis of the pancreatic β cell, causing a reduction ininsulin levels and then diabetes, leading to neuropathy (diabetic) andneuropathic pain (R. B. Weiss, Cancer Treat. Rep., 66, 427-438 1982, Guyet al, Diabetologica, 28, 131-137 1985; Ziegler et al, Pain, 34, 1-101988; Archer et al, J. Neural. Neurosurgeon. Psychiatry, 46, 491-4991983). The diabetic rat model has been shown to be a reliable model ofhyperalgesia. We have used an STZ-induced diabetic rat model to create astate of hyperlagesia that can be compared with control animals(Courteix et al, Pain, 53, 81-88 1993).

[0181] Three models of neuropathic and/or central sensitization pain inrats, which involve nerve injury, may be used, see Ralston, DD (1998)Present models of neuropathic pain. Pain Rev. 5: 83-100. The injuriesare caused by (1) loosely tying four chromic gut sutures around thesciatic nerve (CCI model developed by Bennett, G J and Y -K Xie, Aperipheral mononeuropathy in rat that produces disorders of painsensation like those seen in man, Pain 33: 87-107 (1988), (2) tightlyligating one third to one half of the fibers in the sciatic nerve (modeldeveloped by Seltzer, Z, R Dubner, Y Shire, A novel behavioral model ofneuropathic pain disorders produced in rats by partial sciatic nerveinjury, Pain 43: 205-218, 1990), and (3) tightly ligating the dorsalspinal nerve of a rat at the L5 or L5 and L6 levels (L5 model developedby Kim, S H and J M Chung, An experimental model for peripheralneuropathy produced by segmental spinal nerve ligation in the rat, Pain50:355-363, 1992).

[0182] (b) Extraction of Nucleic Acids from Neuronal Tissue of Test andControl Animals

[0183] The inventors have determined that RNA extraction of whole spinalcord nervous tissue would provide a way of identifying nucleic acidsequences whose expression in spinal tissue is modulated bystreptozotocin induced diabetes or by a mechanical nerve damage modelfor neuropathic and/or central sensitization pain e.g. CCI. Test(subjected to the nociceptive stimulus) and control animals weresacrificed, and the tissue to be studied e.g. neural tissue separated.Techniques for so doing vary widely from animal to animal and will befamiliar to skilled persons.

[0184] A cDNA library can be prepared from total RNA extracted fromneural tissue of the test and control animals. Where possible, however,it is preferred to isolate the mRNA from the total RNA of the test andcontrol animals, by affinity chromatography on oligo (dT)-cellulose, andthen reverse transcribe the mRNA from the test and control animals togive test and control cDNA. Converting mRNA from the test and controlanimals to corresponding cDNA may be carried out by any suitable reversetranscription method, e.g. a method as described by Gubler & Hoffman,Gene, 25, 263-269 (1983). If desired a proprietary kit may be used e.g.the CapFinder PCR cDNA Library Construction Kit (Life Technologies)which is based on long-distance PCR and permits the construction of cDNAlibraries from nanograms of total RNA.

[0185] (c) Selective Amplification of Differentially Expressed NucleicAcids

[0186] The reverse transcribed cDNA of the test and control animals issubjected to subtractive hybridisation and amplification so thatdifferentially expressed sequences become selectively amplified andcommonly expressed sequences become suppressed, so as to over-produceDNA associated with said nociceptic stimulus. A wide range ofsubtractive hybridisation methods can be used, but the preferred methodis so-called suppression subtractive hybridisation, see U.S. Pat. No.5,565,340 and Diatchenko et al, Proc. Nat. Acad. Sci. USA, 93, 6025-6030(1996), the disclosures of which are herein incorporated by reference.Kits for carrying out this method are available from CLONTECHLaboratories, Inc.

[0187] (d) Cloning and Sequencing the Differentially Expressed cDNA

[0188] The differentially expressed cDNA is ligated into a cloningvector, after which cells of E. coli are transformed with the vector andcultured. Positive clones are selected and lysed to release plasmidscontaining the cDNA insert. The plasmids are primed using forward andreverse primers to either side of the cloning site and the cDNA insertis sequenced. Vector and adaptor sequences are then removed from theoutput data from the sequencer, leaving only the nucleotide sequence ofthe differentially expressed gene. The sequence is then checked againstdata held in a database for homology to known nucleotide sequences andgenes, including expressed sequence tags (ESTs) and coding sequences forproteins.

[0189] (e) Validation of the Above Method

[0190] The importance of the sequences that we have identified in painis confirmed by the fact that genes have been identified using thismethod that represent nucleic acid sequences which have previously beenimplicated in pain, including Calmodulin (pRCM1, Genebank X13933),Enkephalin (Genebank Y07503) and Neurotensin receptor type 2 (GenebankX97121).

[0191] The inventors have identified nucleic acid sequences of the MAPkinase pathway, a previously non pain-associated biological pathway. Theinventors have subsequently shown that intra-spinal injection of a MEKinhibitor (MEK is part of the MAP kinase pathway) produces a powerfulinhibition of pain (Patent application No. U.S. 60/144292).Subsequently, it has been shown that the MAP kinase is also implicatedin acute inflammatory pain (Woolf et al, Nature Neuroscience 1999).

[0192] The invention will now be further described in the followingExample.

EXAMPLE

[0193] Induction of Diabetes

[0194] Diabetes was induced in adult (150-200 g) male Sprague-Dawleyrats (n=6) as described by Courteix et al (supra). Animals were injectedintraperitoneally with streptozotocin (STZ)(50 mg/kg) dissolved indistilled water. Control or sham animals (age-matched animals, n=6) wereinjected with distilled water only.

[0195] Chronic Constrictive Injury (CCI)

[0196] Rats were anaesthetized with i.p. sodium phenobarbital, afterwhich the common left sciatic nerve was exposed at the level of themiddle of the thigh by blunt dissection through the biceps femoris andproximal to the sciatic trifurcation. Four ligatures (4.0 braided silk)were tied loosely around it with about 1 mm spacing. The muscle wasclosed in layers and two wound clips were applied to close the skinincision. The wound was then covered with topical antibiotics.

[0197] Nociceptive Testing

[0198] Static allodynia (a form of hyperlagesia) was measured using amethod described by Chaplan let al, “Quantitative assessment of tactileallodinya in the rat paw”, J. Neurosci. Methods, 53, 55-63 (1994). Aseries of von Frey filaments of different buckling weight (i.e. the loadrequired for the filament to bend) were applied to the plantar surfaceof the right hand paw. The starting filament had a buckling weight of 20g. Lifting of the paw was taken to be a positive result, in which case afilament with the next lowest buckling weight was used for the nextmeasurement. The test was continued until a filament was found for whichthere was an absence of response for longer than 5 seconds whereas are-test with the next heaviest filament gave a positive response.Animals were considered hyperalgesic if their thresholds were found tobe <4 g of those of comparable untreated rats, see Calcutt & Chaplan,“Spinal pharmacology of tactile allodynia in diabetic rats”, British J.Pharmacol, 122, 1478-1482 (1997).

[0199] Tissue Extraction

[0200] STZ-treated, CCI-treated and control animals were anaesthetizedwith 4% halothane and perfused with ice-cold 0.9% saline containing 1%citric acid (pH adjusted to 7.4 with NaOH). The animals were decapitatedand the lumbar spinal cord exposed. A 2-centimetre length of spinal cordending at L6 (lumbar-6 forward) was removed from the spinal column.Attached dorsal root ganglia and contaminating spinal connective tissueswere removed. The spinal cord tissue was snap frozen in dry ice andisopentane. In the experiments that follow, procedures on thestreptozocin-treated and control groups of animals are disclosed. Itwill be understood that for identification of CCI-treated animals thesame experiments are performed, but using tissues from the CCI-treatedanimals and from control animals.

[0201] Total RNA Extraction

[0202] Total RNA was extracted from the pooled male rat tissues of thestreptozocin-treated and control groups using the TRIZOL Reagent Kit(Life Technologies). Briefly, tissue samples were homogenised fullyusing a Polytron homogenizer in 1 ml of TRIZOL reagent per 50-100 mg oftissue. Homogenized samples were then incubated at room temperature for5 minutes and phase separated using 0.2 ml chloroform per 1 ml TRIZOLreagent followed by centrifugation at 3,000 g. The aqueous phase wastransferred to a fresh tube and the RNA precipitated with an equalvolume of isopropyl alcohol and followed by centrifugation at 10,000 g.The RNA pellet was washed in 75% ethanol and re-centrifuged. The pelletwas then air dried and re-suspended in water.

[0203] mRNA Extraction

[0204] In contrast to ribosomal RNA and transfer RNA, the vast majorityof mRNAs of mammalian cells carry tracts of poly(A⁺) at their 3′termini. mRNAs can therefore be separated from the bulk of cellular RNAby affinity chromatography on oligo (dT)-cellulose. mRNA was extractedfrom Total RNA using the MESSAGEMAKER Kit (Life Technologies) in whichmRNA (previously heated to 65° C. in order to disrupt secondarystructures and so expose the poly (A⁺) tails) was bound to oligo(dT)cellulose under high salt concentrations (0.5M NaCl) in a filtersyringe. Unbound RNA was then washed away and the poly(A⁺) mRNA elutedin distilled water. A tenth of the volume of 7.5 M Ammonium Acetate, 50μg of glycogen/ml mRNA sample and 2 volumes of absolute alcohol werethen added to the samples which were placed at −20° C. overnight.Following precipitation, the mRNA was spun down at 12,000 g for 30minutes at 4° C. RNAase free water was used to re-suspend the pellets,which were then and stored at −80° C.

[0205] PCR Select

[0206] The technique used was based on that of the CLONTECH PCR SelectSubtraction Kit. The following protocol was performed using STZ-treatedlumbar spinal cord Poly A⁺ RNA as the ‘Tester’ and Sham lumbar spinalcord poly A⁺ RNA as the ‘Driver’ (Forward Subtraction). A secondsubtraction experiment using the Sham lumbar spinal cord mRNA as the‘Tester’ and STZ treated lumbar spinal cord mRNA as the ‘Driver’(Reverse Subtraction) was performed in parallel using the same reagentsand protocol. As a control for both experiments, the subtraction wasalso carried out using human skeletal muscle mRNA that had been providedby the manufacturer.

[0207] First-Strand cDNA Synthesis

[0208] 2 μg of PolyA⁺ RNA and 1 μl of cDNA synthesis primer (10 μM) werecombined in a 0.5 ml Eppendorf tube and sterile H₂O was added wherenecessary to achieve a final volume of 5 μl. The contents were mixedgently and incubated in a thermal cycler at 70° C. for 2 min. The tubeswere then cooled on ice for two minutes, after which 2 μl of5×First-strand buffer, 1 μl of dNTP mix (10 mM each), sterile H₂O and 1μl of AMV reverse transcriptase (20 units/μl) was also added. The tubeswere then placed at 42° C. for 1.5 hr in an air incubator. First-strandcDNA synthesis was terminated by placing the tubes on ice. (the humanskeletal muscle cDNA produced by this step was used as the ‘controldriver’ in later steps).

[0209] Second-Strand cDNA Synthesis

[0210] 48.4 μl of Sterile H₂O, 16.0 μl of 5×Second-strand buffer, 1.6 μlof dNTP mix (10 mM) and 4.0 μl of 20×Second-strand enzyme cocktail wereadded to each of the first-strand synthesis reaction tubes. The contentswere then mixed and incubated at 16° C. in a thermal cycler for 2 hr. 6units (2 μl) of T4 DNA polymerase was then introduced and the tubes wereincubated for a further 30 min at 16° C. In order to terminatesecond-strand synthesis, 4 μl of 20×EDTA/glycogen mix was added. Aphenol:chloroform extraction was then carried out using the followingprotocol:-

[0211] 100 μl of phenol:chloroform:isoamyl alcohol (25:24:1) was addedto the tubes which were then vortexed thoroughly and centrifuged at14,000 rpm for 10 min at room temperature. The top aqueous layer wasremoved and placed in a fresh tube. 100 μl of chloroform:isoamyl alcohol(24:1) was then added to the aqueous layer and the tubes were againvortexed and centrifuged at 14,000 rpm for 10 min. 40 μl of 4 M NH₄OAcand 300 μl of 95% ethanol were then added and the tubes centrifuged at14,000 rpm for 20 min. The supernatant was removed carefully, then 500μl of 80% ethanol was added to the pellet. The tubes were centrifuged at14,000 rpm for 10 min and the supernatant was removed so that the pelletcould be air-dried. The precipitate was then dissolved in 50 μl of H₂O.6 μl was transferred to a fresh microcentrifuge tube. The remainder ofthe sample was stored at −20° C. until needed.

[0212] Rsa I Digestion

[0213] All products of the above procedures were subjected to arestriction digest, using the restriction endonuclease Rsa I, in orderto generate ds cDNA fragments that are short and thus are optimal forsubtraction hybridisation due to the standard kinetics of thehybridisation. Also, as Rsa I makes a double stranded cut in the middleof a recognition sequence, ‘blunt ends’ of a known nucleotide sequenceare produced allowing ligation of adaptors onto these ends in a laterstep. The following reagents were added to the 6 μl product of thesecond hybridisation (see above): 43.5 μl of ds cDNA, 5.0 μl 10×Rsa Irestriction buffer and 1.5 μl of Rsa I (10 units/μl). The reactionmixture was incubated at 37° C. for 1.5 hr. 2.5 μl of 20×EDTA/glycogenmix was used to terminate the reaction. A phenol:chloroform extractionwas then performed as above (second-strand cDNA synthesis section). Thepellet produced was then dissolved in 5.5 μl of H₂O and stored at −20°C. until needed. The preparation of the experimental ‘Driver’ cDNAs andthe control skeletal muscle cDNA was thus completed.

[0214] Adaptor Ligation

[0215] The adaptors were not ligated to the driver cDNA.

[0216] 1 μl of each Rsa I-digested experimental cDNA (from the Rsa IDigestion above) was diluted with 5 μl of sterile water. Preparation ofthe control skeletal muscle tester cDNA was then undertaken by brieflycentrifuging the tube containing control DNA (Hae III-digest of φX174DNA [3 ng/μl]) and diluting 2 μl of the DNA with 38 μl of sterile water(to 150 ng/ml). 1 μl of control skeletal muscle cDNA (from the Rsa IDigestion) was then mixed with 5 μl of the diluted φX174/Hae III DNA(150 ng/ml) in order to produce the control skeletal muscle tester cDNA.

[0217] Preparation of the Adaptor-Ligated Tester cDNA

[0218] A ligation master mix was prepared by combining 3 μl of sterilewater, 2 μl of 5×ligation buffer and 1 μl T4 DNA ligase (400 units/μl)per reaction. 2 μl of adaptor 1 (10 μM) was then added to 2 μl of thediluted tester cDNA. To this, 6 μl of the ligation master mix was alsoadded. The tube was therefore labeled Tester 1-1. In a separate tube, 2μl of the adaptor 2R (10 μM) was mixed with 2 μl of the diluted testercDNA and 6 μl of the master mix. This tube was named Tester 1-2.

[0219] 2 μl of Tester 1-1 and 2 μl of Tester 1-2 were then placed intofresh tubes. These would later be used as the unsubtracted testercontrol. The remainder of the contents of Tester 1-1 and Tester 1-2tubes were then centrifuged briefly and incubated at 16° C. overnight.The ligation reaction was stopped by adding 1 μl of EDTA/glycogen mixand the samples were heated at 72° C. for 5 min in order to inactivatethe ligase. In doing so, preparation of the experimental and controlskeletal muscle adaptor-ligated tester cDNAs was complete.

[0220] 1 μl from each unsubtracted tester control was then removed anddiluted into 1 ml of water. These samples were set aside as they were tobe used later for PCR (see below). All of the samples were stored at−20° C.

[0221] Analysis of Ligation Efficiency

[0222] 1 μl of each ligated cDNA was diluted into 200 μl of water andthe following reagents were then combined in four separate tubes: Tube:Component 1 2 3 4 Tester 1-1 (ligated to Adaptor 1) 1 1 — — Tester 1-2(ligated to Adaptor 2R) — — 1 1 G3PDH 3′ primer (10 μM) 1 1 1 1 G3PDH 5′primer (10 μM) — 1 — 1 PCR primer 1 (10 μM) 1 — 1 — Total volume μl 3 33 3

[0223] A master mix for all of the reaction tubes plus one additionaltube was made up by adding 18.5 μl of sterile H₂O, 2.5 μl of 10×PCRreaction buffer, 0.5 μl of dNTP mix (10 mM), and 0.5 μl of 50×AdvantagecDNA Polymerase Mix, per reaction, into a fresh tube. 22 μl of thismaster mix was then aliquotted into each of the 4 reaction tubesprepared above. The contents of the tubes were overlaid with 50 μl ofmineral oil. The reaction mix was incubated in a thermal cycler at 75°C. for 5 min in order to extend the adaptors. The following protocol wasthen carried out immediately in a thermal cycler (Perkin-Elmer GeneAmpPCR Systems 2400): 94° C. for 30 sec (1 cycle), 94° C. 10 sec, 65° C. 30sec and then 68° C. 2.5 min (25 cycles)

[0224] First Hybridisation

[0225] 1.5 μl of the Adaptor 1-ligated Tester 1-1 was combined with 1.5μl of the Rsa I-digested driver cDNA, prepared earlier and 1 μl of4×Hybridisation buffer. This process was then repeated combining theAdaptor 2R-ligated Tester 1-2 with the Rsa I-digested driver cDNA and4×hybridisation buffer. The samples were incubated in a thermal cyclerat 98° C. for 1.5 min followed by incubation at 68° C. for 8 hr.

[0226] Second Hybridisation

[0227] 1 μl of Driver cDNA (i.e. the Rsa I-digested cDNA (see above)), 1μl 4×Hybridisation buffer and 2 μl Sterile H₂O were all combined in afresh tube. 1 μl of this mix was then removed and placed in a new tube,overlaid with 1 drop of mineral oil and incubated at 98° C. for 1.5 minin order to denature the driver. The following procedure was used tosimultaneously mix the driver with hybridisation samples 1 and 2(prepared in the first hybridisation), thus ensuring that the twohybridisation samples were mixed together only in the presence offreshly denatured driver: A micropipettor was set at 15 μl. The pipettetip was then touched onto the mineral oil/sample interface of the tubecontaining hybridisation sample 2. The entire sample was drawn partwayinto the tip before it was removed from the tube in order to draw asmall amount of air into the tip. The pipette tip was then touched ontothe interface of the tube containing the freshly denatured driver (i.e.the tip contained both samples separated by a small pocket of air)before the entire mixture was transferred to the tube containinghybridisation sample 1. The reaction was then incubated at 68° C.overnight. 200 μl of dilution buffer was added to the tube, which wasthen heated in a thermal cycler at 68° C. for 7 min. The product of thissecond hybridisation was stored at −20° C.

[0228] PCR Amplification

[0229] Seven PCR reactions were set up: (1) The forward-subtractedexperimental cDNA, (2) the unsubtracted tester control (see preparationof the adaptor ligated tester cDNA), (3) the reverse-subtractedexperimental cDNA, (4) the unsubtracted tester control for the reversesubtraction, (5) the subtracted control skeletal muscle cDNA, (6) theunsubtracted tester control for the control subtraction, and (7) the PCRcontrol subtracted cDNA (provided in the kit). The PCR controlsubtracted cDNA was required to provide a positive PCR control as itcontained a successfully subtracted mixture of Hae III-digested φX174DNA.

[0230] The PCR templates were prepared by aliquotting 1 μl of eachdiluted cDNA (i.e., each subtracted sample from the second hybridisationand the corresponding diluted unsubtracted tester control produced bythe adaptor ligation see above) into an appropriately labeled tube. 1 μlof the PCR control subtracted cDNA was placed into a fresh tube. Amaster mix for all of the primary PCR tubes, plus one additionalreaction, was then prepared by combining 19.5 μl of sterile water, 2.5μl of 10×PCR reaction buffer, 0.5 μl of dNTP Mix (10 mM), 1.0 μl of PCRprimer 1 (10 μM) and 0.5 μl of 50×Advantage cDNA Polymerase Mix. 24 μlof Master Mix was then aliquotted into each of the 7 reaction tubesprepared above and the mixture was overlaid with 50 μl of mineral oil,before being incubated in a thermal cycler at 75° C. for 5 min in orderto extend the adaptors. Thermal cycling was then immediately startedusing the following protocol: 94° C. 25 sec (1 cycle), 94° C. 10 sec,66° C. 30 sec and 72° C. 1.5 min (32 cycles).

[0231] 3 μl of each primary PCR mixture was then diluted in 27 μl ofH₂O, 1 μl of each of these dilutions was then placed into a fresh tube.

[0232] A master mix for the secondary PCRs, (plus an additionalreaction) was set up by combining 18.5 μl of sterile water, 2.5 μl of10×PCR reaction buffer, 1.0 μl of Nested PCR primer 1 (10 μM), 1.0 μl ofNested PCR primer 2R (10 μM), 0.5 μl of dNTP Mix (10 mM) and 0.5 μl of50×Advantage cDNA Polymerase Mix per reaction. 24 μl of this Master Mixwas then added into each reaction tube containing the 1 μl dilutedprimary PCR mixture. The following PCR protocol was then carried out:94° C. 10 sec, 68° C. 30 sec and 72° C. 1.5 min (12 cycles). Thereaction products were then stored at −20° C.

[0233] Ligation Into a Vector/Transformation & PCR

[0234] The products of the PCR amplification (enriched fordifferentially expressed cDNAs) were ligated into the pCR2.1-TOPO vectorusing a T/A cloning kit (Invitrogen), transformed into TOPO One Shotcompetent cells according to the manufacturers protocol and grown up onLB (Luria-Bertani) Agar plates overnight at 37° C. 1,000 colonies werethen individually picked (using fresh sterile tips) and dipped into 5 μlof sterile water which had been aliquotted previously into 96 well PCRplates. The water/colonies were heated in a thermal cycler at 100° C.for 10 minutes in order to burst the cells, thus releasing the plasmidscontaining a differentially expressed cDNA insert. The 5 μl ofwater/plasmid was then used as a template in a PCR reaction (see below)using M13 Forward and Reverse primers (10 ng/μl), complementary to theM13 site present on either side of the cloning site on the vector. 5 μlof the PCR product was then run on a 2% agarose gel and stained byethidium bromide. PCR products of an amplified insert were identifiedand 5 μl of the remainder of the PCR product (i.e. from the 15 μl thathad not been run on the gel) was diluted {fraction (1/10)} with water. 5μl of the diluted PCR product was then used as a template in asequencing reaction.

[0235] Sequencing

[0236] A sequencing reaction containing M13 primer (3.2 pmol/μl),‘BigDye’ reaction mix (i.e. AmpliTaq® DNA polymerase, MgCl₂, buffer andfluorescent dNTPs [each of the four deoxynucleoside triphosphates islinked to a specific fluorescent donor dye which in turn is attached toa specific acceptor dye]) and cDNA template (diluted PCR product) wasset up. The reaction was carried out on a thermal cycler for 25 cyclesof 10 seconds at 96° C., 20 seconds at 50° C. and 4 minutes at 60° C.Each reaction product was then purified through a hydrated Centri-Sepcolumn, and lyophilised. The pellets were resuspended in TemplateSupression Reagent and sequenced on an ABI Prism 310 Genetic Analyser.The analyser uses an ion laser to excite the specific donor dye thattransfers its energy to the acceptor dye, which emits a specific energyspectrum that can be read by the sequencer.

[0237] The differentially expressed genes of the streptozocin-induceddiabetes experiment and of the CCI experiment were sequenced atParke-Davis, Cambridge and at the applicant's core sequencing facilityin Ann Arbor, Mich., USA.

[0238] Bioinformatics

[0239] The sequencing results were analysed using the computer programCHROMAS in which the vector and adaptor sequences were clipped off,leaving only the nucleotide sequence of the differentially expressedgene. Each sequence was then checked for homology to known genes,Expressed Sequence Tags (ESTs) and Proteins using various Basic LocalAlignment Search Tool (BLAST) searches against the Genbank sequencedatabase at the National Centre for Biotechnology Information, Bethesda,Md., USA (NCBI).

[0240] Lists were derived called STZup and STZdown and CCIup and CCIdownthat contain the nucleic acid sequences from the forward and backsubtracted libraries respectively. In each list there are givenaccession numbers and descriptions for the known rat genes identified,and where available corresponding mouse or human genes. Sequences thatare considered to be of interest and that are down-regulated both in astreptozocin-induced diabetes model and in a chronic constrictive injurypain model are identified in Tables I-VI above and are listed below.

[0241] References have been given where available for the sequences thathave been found, and sequence listings have been given in the form inwhich they currently appear in publicly searchable databases e.g. theNCBI databaase (National Center for Biotechnology Information, NationalLibrary of Medicine, National Institutes of Health, Bethesda, Maryland,Md. 20894, USA, www.ncbi.nlm.nih.gov). These sequence listings are givenfor the purposes of identification only. The invention includes the useof subsequently revised versions of the above sequences (which mayincorporate small differences to the version set out herein) andhomologous sequences or similar proteins in other species as determinedby a high percentage identity (e.g. above 50%, preferably above 90%),length of alignment and functional equivalence.

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1 117 1 531 PRT Rattus norvegicus Pyruvate kinase M1 subunit 1 Met ProLys Pro Asp Ser Glu Ala Gly Thr Ala Phe Ile Gln Thr Gln 1 5 10 15 GlnLeu His Ala Ala Met Ala Asp Thr Phe Leu Glu His Met Cys Arg 20 25 30 LeuAsp Ile Asp Ser Ala Pro Ile Thr Ala Arg Asn Thr Gly Ile Ile 35 40 45 CysThr Ile Gly Pro Ala Ser Arg Ser Val Glu Met Leu Lys Glu Met 50 55 60 IleLys Ser Gly Met Asn Val Ala Arg Leu Asn Phe Ser His Gly Thr 65 70 75 80His Glu Tyr His Ala Glu Thr Ile Lys Asn Val Arg Ala Ala Thr Glu 85 90 95Ser Phe Ala Ser Asp Pro Ile Leu Tyr Arg Pro Val Ala Val Ala Leu 100 105110 Asp Thr Lys Gly Pro Glu Ile Arg Thr Gly Leu Ile Lys Gly Ser Gly 115120 125 Thr Ala Glu Val Glu Leu Lys Lys Gly Ala Thr Leu Lys Ile Thr Leu130 135 140 Asp Asn Ala Tyr Met Glu Lys Cys Asp Glu Asn Ile Leu Trp LeuAsp 145 150 155 160 Tyr Lys Asn Ile Cys Lys Val Val Glu Val Gly Ser LysIle Tyr Val 165 170 175 Asp Asp Gly Leu Ile Ser Leu Gln Val Lys Glu LysGly Ala Asp Tyr 180 185 190 Leu Val Thr Glu Val Glu Asn Gly Gly Ser LeuGly Ser Lys Lys Gly 195 200 205 Val Asn Leu Pro Gly Ala Ala Val Asp LeuPro Ala Val Ser Glu Lys 210 215 220 Asp Ile Gln Asp Leu Lys Phe Gly ValGlu Gln Asp Val Asp Met Val 225 230 235 240 Phe Ala Ser Phe Ile Arg LysAla Ala Asp Val His Glu Val Arg Lys 245 250 255 Val Leu Gly Glu Lys GlyLys Asn Ile Lys Ile Ile Ser Lys Ile Glu 260 265 270 Asn His Glu Gly ValArg Arg Phe Asp Glu Ile Leu Glu Ala Ser Asp 275 280 285 Gly Ile Met ValAla Arg Gly Asp Leu Gly Ile Glu Ile Pro Ala Glu 290 295 300 Lys Val PheLeu Ala Gln Lys Met Met Ile Gly Arg Cys Asn Arg Ala 305 310 315 320 GlyLys Pro Val Ile Cys Ala Thr Gln Met Leu Glu Ser Met Ile Lys 325 330 335Lys Pro Arg Pro Thr Arg Ala Glu Gly Ser Asp Val Ala Asn Ala Val 340 345350 Leu Asp Gly Ala Asp Cys Ile Met Leu Ser Gly Glu Thr Ala Lys Gly 355360 365 Asp Tyr Pro Leu Glu Ala Val Arg Met Gln His Leu Ile Ala Arg Glu370 375 380 Ala Glu Ala Ala Val Phe His Arg Leu Leu Phe Glu Glu Leu AlaArg 385 390 395 400 Ala Ser Ser Gln Ser Thr Asp Pro Leu Glu Ala Met AlaMet Gly Ser 405 410 415 Val Glu Ala Ser Tyr Lys Cys Leu Ala Ala Ala LeuIle Val Leu Thr 420 425 430 Glu Ser Gly Arg Ser Ala His Gln Val Ala ArgTyr Arg Pro Arg Ala 435 440 445 Pro Ile Ile Ala Val Thr Arg Asn Pro GlnThr Ala Arg Gln Ala His 450 455 460 Leu Tyr Arg Gly Ile Phe Pro Val LeuCys Lys Asp Ala Val Leu Asp 465 470 475 480 Ala Trp Ala Glu Asp Val AspLeu Arg Val Asn Leu Ala Met Asn Val 485 490 495 Gly Lys Ala Arg Gly PhePhe Lys Lys Gly Asp Val Val Ile Val Leu 500 505 510 Thr Gly Trp Arg ProGly Ser Gly Phe Thr Asn Thr Met Arg Val Val 515 520 525 Pro Val Pro 5302 531 PRT Rattus norvegicus Pyruvate kinase M2 subunit 2 Met Pro Lys ProAsp Ser Glu Ala Gly Thr Ala Phe Ile Gln Thr Gln 1 5 10 15 Gln Leu HisAla Ala Met Ala Asp Thr Phe Leu Glu His Met Cys Arg 20 25 30 Leu Asp IleAsp Ser Ala Pro Ile Thr Ala Arg Asn Thr Gly Ile Ile 35 40 45 Cys Thr IleGly Pro Ala Ser Arg Ser Val Glu Met Leu Lys Glu Met 50 55 60 Ile Lys SerGly Met Asn Val Ala Arg Leu Asn Phe Ser His Gly Thr 65 70 75 80 His GluTyr His Ala Glu Thr Ile Lys Asn Val Arg Ala Ala Thr Glu 85 90 95 Ser PheAla Ser Asp Pro Ile Leu Tyr Arg Pro Val Ala Val Ala Leu 100 105 110 AspThr Lys Gly Pro Glu Ile Arg Thr Gly Leu Ile Lys Gly Ser Gly 115 120 125Thr Ala Glu Val Glu Leu Lys Lys Gly Ala Thr Leu Lys Ile Thr Leu 130 135140 Asp Asn Ala Tyr Met Glu Lys Cys Asp Glu Asn Ile Leu Trp Leu Asp 145150 155 160 Tyr Lys Asn Ile Cys Lys Val Val Glu Val Gly Ser Lys Ile TyrVal 165 170 175 Asp Asp Gly Leu Ile Ser Leu Gln Val Lys Glu Lys Gly AlaAsp Tyr 180 185 190 Leu Val Thr Glu Val Glu Asn Gly Gly Ser Leu Gly SerLys Lys Gly 195 200 205 Val Asn Leu Pro Gly Ala Ala Val Asp Leu Pro AlaVal Ser Glu Lys 210 215 220 Asp Ile Gln Asp Leu Lys Phe Gly Val Glu GlnAsp Val Asp Met Val 225 230 235 240 Phe Ala Ser Phe Ile Arg Lys Ala AlaAsp Val His Glu Val Arg Lys 245 250 255 Val Leu Gly Glu Lys Gly Lys AsnIle Lys Ile Ile Ser Lys Ile Glu 260 265 270 Asn His Glu Gly Val Arg ArgPhe Asp Glu Ile Leu Glu Ala Ser Asp 275 280 285 Gly Ile Met Val Ala ArgGly Asp Leu Gly Ile Glu Ile Pro Ala Glu 290 295 300 Lys Val Phe Leu AlaGln Lys Met Met Ile Gly Arg Cys Asn Arg Ala 305 310 315 320 Gly Lys ProVal Ile Cys Ala Thr Gln Met Leu Glu Ser Met Ile Lys 325 330 335 Lys ProArg Pro Thr Arg Ala Glu Gly Ser Asp Val Ala Asn Ala Val 340 345 350 LeuAsp Gly Ala Asp Cys Ile Met Leu Ser Gly Glu Thr Ala Lys Gly 355 360 365Asp Tyr Pro Leu Glu Ala Val Arg Met Gln His Leu Ile Ala Arg Glu 370 375380 Ala Glu Ala Ala Ile Tyr His Leu Gln Leu Phe Glu Glu Leu Arg Arg 385390 395 400 Leu Ala Pro Ile Thr Ser Asp Pro Thr Glu Ala Ala Ala Val GlyAla 405 410 415 Val Glu Ala Ser Phe Lys Cys Cys Ser Gly Ala Ile Ile ValLeu Thr 420 425 430 Lys Ser Gly Arg Ser Ala His Gln Val Ala Arg Tyr ArgPro Arg Ala 435 440 445 Pro Ile Ile Ala Val Thr Arg Asn Pro Gln Thr AlaArg Gln Ala His 450 455 460 Leu Tyr Arg Gly Ile Phe Pro Val Leu Cys LysAsp Ala Val Leu Asp 465 470 475 480 Ala Trp Ala Glu Asp Val Asp Leu ArgVal Asn Leu Ala Met Asn Val 485 490 495 Gly Lys Ala Arg Gly Phe Phe LysLys Gly Asp Val Val Ile Val Leu 500 505 510 Thr Gly Trp Arg Pro Gly SerGly Phe Thr Asn Thr Met Arg Val Val 515 520 525 Pro Val Pro 530 3 11788DNA Rattus norvegicus Pyruvate kinase 3 cccgggcgag cgccgggagg gtggagagtcaccgggcggg gctggaggaa tgtccgggac 60 ctataaatct gggcaacgcc ctggtaggccagggcagatg gggcacctgg gcagaattcc 120 aaaatgggat tatgtagcct ctgaggtcctaaagcaacag gtggcggacc acccggggat 180 ctaggggtgg tggcggcggt ggacccgagggcgggtcctg cctcctcacc acttccccat 240 tggccatcag aatgacccat gcgcaattttggtttgcaat gtccttccgc cacggaaggt 300 agtccccctc aaaagggcaa cctgcttgtcccgcctaccc tgcgactctc tcagaaggtg 360 cgggtgcctg ttgagaggcg gggctctgctagctgctgcc cggattgggc gaggggcggg 420 gctgcggagg gattgcggcg gcccgcagcagtgataacct tgaggcccag tctgcgcagc 480 cccgcacagc agcgacccgt cctaagtcgacagacgtcct ctttaggtat tgcaacagga 540 tctgaagtac gcccgaggtg agcggggagaacctttgcca ttctgtggcc cagagccaat 600 gccatagtgg atcactagtg ggtggctgcaatgtcaggtg gcagagccag gcttgggtgc 660 atgttcttta ggtatccaga aaagccgatgtccagggtag agtgcgggtc tttgggaagg 720 cagggaagag caaggagatg tggagtgcaggctgtgtgct caggagtttg aaaggagcct 780 cgtggcctga aaattgattc aagtctctggtattctgtga ggtacatccc aggttctcgc 840 ttactcagcc aactatccag ctcagtgagtgcttagctgt aagtcgtaat aaagtaaaag 900 caaaggtcag gacagctgtt gggattgtgacctgggtgat taattgcctg gaacagatgt 960 tcagactact gtgtcgtggt ccctatcagacagagaagga gactctagat agccagaact 1020 ggggctggga aagctgggtc taatgtcagctcttgggaag agtagtgact ttcaaggtca 1080 acctcggttg cacagtggat ttcaggccagcatagactag ctactgtgtg tgattccatc 1140 tcctaggtta ctgaagactc cttggtgaggttgtaatgac tttggagcca tttcaatgtc 1200 cttgtgcaaa atttgctttg tgtgtcttgtacatggtgct gcttttttgc ttcttgtcca 1260 gaaaaaaact tgttgcctgt aatttggaccttattcctgt gtcggataga ggctaaggaa 1320 tacatttagg tgatacctca aacctaggactttaaaaaga aaggacttgg gacagggttc 1380 gtctcttggc agcaggatcg tttaggtcatggcgaacatg ataaagaggc tatgtcagtg 1440 ccaaagcagg tttgacattg tcgtgataggcttcccagat gttcgtgtgc tgacacatta 1500 gctacgtgct ctgcaggagt ggggaggtttccttgtgtgg gaaggtatgt tctctctagg 1560 gttggtgtga agctcttcaa tagatggcagccgttgtaag aagctctgct taaactggca 1620 gccagggtgt gtatgtgcac tgggaaggaagctacttttg ttcaccaagg tctttgctgt 1680 tcgttcatag tcacaggagc cctttgcccaatttgagtag tacccacata accagcacgt 1740 gccaggcctc tgagtgactt gtgcacaaagcagtcttatt gggttaatgt gtaagcggga 1800 gacagtctcc tatgccaaat ggttcattaagcactgccta gtctaattga actcttcaaa 1860 tctccgggat ccaggatctc agaaaccatgcccaagccag acagcgaagc agggactgcc 1920 ttcattcaga cccagcagct ccatgcagccatggctgaca ccttcctgga acacatgtgc 1980 cgcctggaca ttgactccgc acccatcacggcccgcaaca ctgggatcat ctgtaccatt 2040 ggtgagtgag tgtggccccc ttcgcagggcttctgcctgg tttgaaaggc gtaataacca 2100 ttgcagggct aacctaggtt ctgagagacatgtccacact tttagaggaa cgtattggtg 2160 tacttcttct gcacttggtg aggttcagggtggtctagtg gcctttgcag gagtagttca 2220 gggacttcag atctttttcc tgtgctgtcactccactttc tgcagaattt atagaaaatg 2280 atagtgtcct tccttacata taaattaagagagtccttcc ttacatataa atcaagagag 2340 ttgactagca cagtagtctt tctggactttggaaagacct ttctctaaat ttgtggaggt 2400 attaaaaaca aaggacaagg agttgcctttgatagtagca gatttcatat actttcctac 2460 ctgagaatca tgattttctt cctgtggctctaaatgtttc ttggtaggcc ctgcttcccg 2520 atctgtggag atgctgaagg agatgattaagtctgggatg aatgtggctc ggctgaattt 2580 ctctcatgga acccatgagg tgagtggcagcttgatccag gaggttcggg acttgctgct 2640 gctgtgctcc aggcttctca gtgaatggacgttgctctac ttacatttag atcccctctc 2700 tccctcttcc cctctggcca acttcattggcatcaacaac ctgcctgctt tgtcgccggc 2760 caggttatcc ctaccatcaa gcttcaccagggctttctta ttctatgtgc accttcgatg 2820 aggggctgtg gctgtctgtc ttggatgaggggctgtggct gtctgtcttg gttttaacca 2880 ttcctggaga cggtggaatg aatcgtagctccctcgagct cttggcctaa ttgatcatct 2940 ttggctccag ggtggctcag ggcatgggaactggacttgg gtgtggagaa cacctacctg 3000 tcaatctccc cttctttctc tccagtaccatgcagagact atcaagaatg tccgtgcagc 3060 cacagaaagc tttgcatctg atcccattctctaccgacct gttgcggtgg ctctggatac 3120 aaagggacct gagatccgga ctggactcatcaagggcgtg agtatccagg agtttaggtc 3180 tttaaatgag aatatttttc atctgcctggtaggaattat tatagatgca catttttggt 3240 atgtgaataa catacttaag tctcactctggggacctggt ttgttgtttg ttttgtttgt 3300 ttccctcaat aaacaaattc aggatttacagaaaggtgat cggtttcttg gggctttgag 3360 ccagagtttg agcgccgcca tcagggtgttggcgtccaca gtcacacgcc tctgctgtct 3420 ttaatctaga gcggcaccgc agaggtggagctgaagaagg gagccacact gaagatcacc 3480 ctggacaacg cctacatgga gaagtgcgacgagaacatcc tgtggctgga ctataagaac 3540 atctgcaagg tggtggaggt gggcagcaagatctacgtgg acgatgggct catctccctg 3600 caggtgaagg agaaaggtat gtgtggtgtacagtccacgg cccaatgcca ctcccatccc 3660 cagaactctg gtaagcactt aacctagcatgtatgaattg gtctcccaag gtcaaaagtt 3720 taaggtggtt gttggtctgc atccctggcctgtctgaaac actgcctgag aaaaaataga 3780 caaataacct acaaaggcct atgtgtacacctctaccctt tagttccagc actcgggaat 3840 cagcaggtgt gtgagttctc atgtgtaaggactactcctg tatgcctaga atgagtctag 3900 agttctcttg gcttctcaca aactgagatagatggtcttg atccctttca cacaggtgct 3960 gactacctgg tgacagaagt ggaaaatggtggctccttgg gcagcaagaa gggcgtgaac 4020 ctgcctggtg ctgctgtgga cctccctgctgtgtcagaaa aggacatcca ggacctgaag 4080 tttggggtgg agcaggacgt ggacatggtgtttgcgtctt tcatccgcaa ggcggctgac 4140 gtgcatgagg ttaggaaggt cctgggagagaagggcaaga acatcaagat catcagcaaa 4200 atcgagaacc atgaaggtgt ccgcaggtgaaggtccactc ctaccgtgtg cctggggtgt 4260 ggccttgagg gcacctctgt cagggcccaggaaagctctg tccattcttt ggttgtactt 4320 cctgttctat agcatctttc tttttatcaggtttgatgag attttggagg ccagcgatgg 4380 aatcatggta gctcgtggtg acctgggcattgagattccg gcagagaagg tcttcctagc 4440 tcagaagatg atgattggac gatgcaaccgagctgggaag ccagtcatct gcgccaccca 4500 ggcatgtgct atcccttcct tctgtgttctccacctagga gacctggtct tgacctggcc 4560 tttaggtaca cgtacccgca catagctatgacctgcgtac ctgtgcaagc ttcggggaat 4620 tgccctggaa tcatcactga agatgtcctgctcttccatt atttagtgac tttcatttag 4680 cggtggtctc ttacttaata aaaacccttgtttgtcccct cctagatgct ggagagcatg 4740 atcaagaagc cacgccccac ccgtgctgaaggcagtgacg tggccaatgc agtcctagat 4800 ggagctgact gcatcatgct gtccggagaaacagccaaag gggactaccc tctggaggct 4860 gttcgcatgc agcacctggt gagtaagtcctcagagtcct ggggtagaag agctctgctg 4920 gagaggcctc tgtccagtct tgttacattgctcccgtcac agcaaggaga gtgaggtttg 4980 tggagtgtgc ttgagtttca ttgtgctttcactgcctgca cctgcccctt tgtcctgctc 5040 tggggattac ataggccaca tctggctaaaatatcaaggt cctaggatgc agtcaaggga 5100 tgccttcctt gtggacaccc agagggcctgggtacctcta ttctaaagga gccaagagtt 5160 tgttcagcta ttcgccttgt tacctctcatttggtctcct gtggtctgcc catgggcaat 5220 gcttctctcc actgcagctg tagccatactgagctgcttt aagctggccg tgcatggtgc 5280 ctgtgacatg ggacttcctc ccttgctgtgccagacccaa ctcggggcta caaatagctc 5340 tgggggtggg gaatgggtgc taatttagcaggttctgtct ggtctaggaa ttataaagac 5400 ttctcaggca tattatgtgc tcttttgattaagtcttctg gtttcagtaa gatagggtct 5460 ggcgcaagct tgtaattcca acaatcaaatcaagcctgcg gcaggaagat ggcttgagat 5520 taggctggac tagaatgaga catcttgtagaagcaacagg cgtcagtgac ctgtccctgc 5580 ggactttcca cccaggcctc cgtcttctgtgttctgctcc agaacttctt ccggagcaga 5640 acaattatct ggggcatcag ttagggaccgttgcctgata aaggcatggt ggattagctt 5700 tttgggggtt ttctattccc ttttcagccaacaaagaacc acagtatctt ttgtggctcc 5760 agtttcccac aagccccccc aaacttaagacaagtgaaag aaaagaagga agccccttct 5820 gggttaggca tcttaccttg ctttatatgtatatgagagt tgagtagatt ccatgatctt 5880 tccatggatt tatctgaatt atagctgtagccagatgttt gcctatattg aaacagacca 5940 gcgctttgac tcggctggtc atgacctgttctgattggaa ttgtgactgg tcctcatagc 6000 ttctcatagc tgcaggcatt ctctcccagaagacttttcc cttccttcaa tctcccccac 6060 agtagctgcc ttcctggttt acaccaaaaccccttttcta cttacctggc ctcacagtgg 6120 tcaggaacag gactttgacc aggtattctaaagcatgtag tcacataaat gtatttttgg 6180 atagctcaag aagaccttgc ccaaagtggcggttgatgag attaattaca attaattata 6240 tgtgtatgag ttagttttgg tgtcctgtccttttctcatg ccctggtctt tcctatgaac 6300 agacacctta gaacctcgag gctgggattgcatggccctg ctcagaagat gagtcacaga 6360 gtccgggtta gactgtggct accccctcagggatacaaat ttctctatca gttaacactt 6420 aggacagctt ctcccctttc tttatctgtttgctgtttcc tcctgtgtct aactgattca 6480 gttcaaaacc tgtaattaaa agctagacatcccagctgtg gttgcttcct gtccatgcct 6540 cttgtccttt gggcttgcct gcattccatgcttaaccaaa agattacttt ccacttgcaa 6600 atctgctaat gctaccaata aaatcgagtgctggtttcat atcattcctg gaattgcact 6660 ctctaaaact ttatttcttt atttctaatgcttggtttta cttgaacagg tggtctgtat 6720 ataacacatt tgctatcctg taaccgttttaattattgca gtttgaatct gtgtcttgaa 6780 aggcctgtgt gctttcccag gcgtctgcctcctcacatgg ctgttcagtg taccgtgttc 6840 aagtgagcca gtcgaccact gttctgtttagaacttgtgc actgcaactt tggctctttt 6900 gaccctcacc cccagctttc agagctgcccgagtgtttcc actgtaagcc aagtgcaagc 6960 gctcactctt gtgtgtaggc ggagttggatgccttgtaac cacatagcca tgtgaggagg 7020 ggacgccttt ttcttcctgt aagctgtgtcaggaggcagt gtggtcaagc ggaagtgtag 7080 ttggctccac cttggcatct ttccatgccagggtcccttt ctcagcttta atcaaaaaca 7140 aaagaaatga tggatggtgc atgcctagcacttgggaggc tgaggcaaag aaaatagtgg 7200 tgttttgagg ctgaccatgg caagttcaaggccagtaggt gctatggtaa gatcctatct 7260 caaacctgtg tggggttgag aattggctttttttgtttgt ttgtttttag aaacaggaaa 7320 tcccaaaagg atactatctt tccagaagggaaggaaggat ctgtccgtgt gtttgaacag 7380 aaaagagagc cacccaaaat cccacatataataccattcc aggctttgac catcctgcct 7440 ctgtatctgc atggagaaga aaagattaacctaagagttc cttcctctca ttagttccct 7500 gtctttccat gtgttgtctc ttgtttttgccttcatcctt cttccttatc cttcctaccc 7560 taaaccttac agatagctcg agaggctgaggcagccgtgt tccaccgcct gctgtttgaa 7620 gagcttgcgc gagcctccag tcaatccacagaccccctgg aggccatggc catgggcagc 7680 gtggaggcct cttataaatg tttagcagcagctttgatag ttctgacgga gtccggcagg 7740 tagggccctg agggcaggta tcattataagataaccagct tctcacacaa ctagggccca 7800 ctgtgtgcct gagcctgggc atagcctctctcctgcagga aggcagccaa ggaggtcacg 7860 atagggcagg accaaaggat tccctagtgggtacagtgga agtcacaggc actggttcag 7920 gatggttccc tgtggagttt ctaatcttgctcaagtttca gaaacgtgtt agtgaactca 7980 tctttctcct ggctttttgt gcccaggacatgttccttcc cagttgcctg tgactcttct 8040 ccttcatttg tgacaaagct ctgacaaagccctgtccccc ttcctcgtcc ctctggacgg 8100 atgttgctcc cctagattgc ccgagaggcagaggctgcca tctaccactt gcagttattc 8160 gaggaactcc gccgcctggc gcccattaccagcgacccca cagaagctgc cgccgtgggt 8220 gccgtggagg cctccttcaa gtgctgcagtggggccatta tcgtgctcac caagtctggc 8280 aggtaggagg cggcagtggc tccctggggatcccagagca actctgggct gattttaaga 8340 cccctggctg ccatcaaagg actccaggaagcactcccag gtacatcaga ttaggtggct 8400 ccagtgccag tcagtgcagg cctgcctcagggcctgaagc atatatacag ttttgcttag 8460 ttacggtgtg gaagctgggc atgggtacatgcctttaatc ctcccagcat tcgggcagaa 8520 gaggtgggca aatgtctgca ttcgtggacagtgctggtct acaaagcaag ttccaggaca 8580 gagccctagc tcacaaaaat taactcagctggtaaatact acttgctaca ccaagttaga 8640 cggatggagg agggagaaag gtaacttagcaagcgtgccc aaggacatat acagtaacac 8700 tttttctcat cccacttgga gggagctcaatggataaagt gttggcaatc tggatttgga 8760 tctggcacct gtgggttttt gttgttgtttgggtttttgt tttttttttt ttttttaagt 8820 cacctggaga agtacgttag gcctttaggagcatggggtt ttggttttta agatttattt 8880 attatatatg aactacactc tccctatcttcagtcgcacc agaagagggt gattacagat 8940 gattgtgtaa gccaccatgt ggttgctgggagttgaactc aggacctctg gaagagcagt 9000 tagtgctctt aaccactgag ccatctctccagccagcact gttctttcag aagcccaaca 9060 catacatgac agctcacact atctaactgcagtcctgggg ggaatctaat gccctcttct 9120 aacttccagc cgtacatgtt tatatgatgcatgtggtcag gatacccaga cagctggagg 9180 gaaaggaagg tgcccaacac acctgtgtgcctgtaatccc agcactcatg gtacagaggt 9240 agaaagatca caagataggt ctaatctgagcaaattctgc aacagcctag atacagaaag 9300 ccaaacacat aaaaatcagt gtgggctgaggatgttgacc tttgtgtgaa ttggccatga 9360 acattgtatg cagctggaag caagggatggggtgctgaaa aatggggacc ttaaaactaa 9420 ttttctgaag tcctgaagtt tcgagagctccaagatacaa gttctatgct aatcctggac 9480 tttctctgaa gagttcctgg ttccttcaaagcccatgtag gccagctaga agcagggtat 9540 tttctgcctg tggaatggcc acatcccctaataccttgtg tgtcctcaag atgcctagtt 9600 aattcagtaa gcctgaaaca gatctgatagaagtaaacat atcatttgct atcggttgag 9660 gagagagggg ctccctacat tcccaaaggatcttgattgg ccagatatta accatgcctg 9720 gtcacaccta gattttccaa aaaaccatagaaattcagag gtttctttta gcaccaagtt 9780 cagctgataa gactcccaaa ggagcagtatagtactctct ggcaaatact gtccactcca 9840 gggtctctgc agataccaga agtcaggagctaggcacatg gtgcttggat tgtaaaagtt 9900 gctggcagct acaggatggt tctggtgagattaggccaga gctgcctcac tgcctgatgg 9960 aagggttcac agtgtgggag ggatcctgccagccgtggtc ctatgggact gcccacactg 10020 agatcaggac aatgagttaa aaaggacaggacaggtctga gggggtggcc aggcactaaa 10080 cagtaattgt aagtgggcaa acctgtggcctagaggtgaa ttagagggtg ctcctttggc 10140 tgactgaaag ggtctcgtga cacaatggccattcttccca ccttctcagg agtgctcacc 10200 aagtggcccg gtaccgccca agggctcctatcattgctgt gacacgcaat ccccagacag 10260 cccgccaggc ccatctgtac cgtggcatcttccctgtgct gtgtaaggat gccgtactgg 10320 atgcctgggc tgaggacgtt gatcttcgtgtgaacttggc catgaatgtt ggtatgtagc 10380 tggaagcaag ggatggggtg ctgggaaatggggagcctaa aactaccatt tcctgaagcc 10440 ctcacccaat tttgggccca aggcaaaattaattgcctca ttaggctctt gtaagattaa 10500 ttggagctac tgccctgtgg ggtggggcacacaagctgtt gtggtctttg ttcctatata 10560 aggttggata tcaagagaca aggaagcagctgaccctgaa cttgggcaag gctggccact 10620 ctagagtcta aactgaatgg tgtccaaaggtccctgcagt ctgagttttg cctcagccct 10680 tgtttaagct aggagacttg caccaccttgtggtaaaaag aagtaactgg caacctgctc 10740 tcctacctga attagaagca atagggcatttgttatcctg cctggttagg cttgtgtctg 10800 ggctgggaga aagcaccacc ttgtggtaggagagagtact ggttgctcag ttaggactgg 10860 gtagggcttt gcagttatcc ccaagtgttatatacctcta ctcaccaacc tccttctccc 10920 ctcaggcaag gcccgaggct tcttcaagaagggagatgtg gtcattgtgc tgactggatg 10980 gcgccctggc tctggcttca ccaacaccatgcgtgtagtg cctgtaccat gatgatcctc 11040 tggagcttct cttctagccc ctgtcccttcccctccccta tcctatccat taggccagca 11100 acgttgtagt gctcactctg ggccatagtgtggcgctggt gggctgggac accaggaaaa 11160 attaatgcct ctgaaacatg caatagagcccagctatttt tcatggccct acttgagcca 11220 ggggtgaagg aggaatgcag gattggaaaccctctgactt tatcacagaa gggcagcatt 11280 atctctgtgt tctttgctcc tgtagaaagttttccagaga attcccagcc ctggcctgga 11340 atcaggagac agcaagaaca gaggctgggggcccagggtt cccatgtaga tgacttttgg 11400 ccctgtccct gacttgcttt cccaacagctttggcctctc tcctcgtgca ctccactgct 11460 gtccctgcag atgttccact ctccacctcgtactctgcag cgtctccagg cctgttgcta 11520 tagtgcccac ctgaatgtca ataaacagcagcggaacgac ctgtgttctt ttcttcctgg 11580 ggggcgtggt tgaggctatc ctctgagcagtcgaaaatga caaccggcct aaaggctcag 11640 cctggagccg agtatatagg ttgcctcctccaacagcacc aagtggggct atctatgtgt 11700 tggaaagctc actgttttat ttcaggacagactggaaagg tctgggacag gttcagctag 11760 cacctgtaat ggtgggtaga ccggaggg11788 4 446 PRT Rattus norvegicus Dopamine receptor D1 4 Met Ala Pro AsnThr Ser Thr Met Asp Glu Ala Gly Leu Pro Ala Glu 1 5 10 15 Arg Asp PheSer Phe Arg Ile Leu Thr Ala Cys Phe Leu Ser Leu Leu 20 25 30 Ile Leu SerThr Leu Leu Gly Asn Thr Leu Val Cys Ala Ala Val Ile 35 40 45 Arg Phe ArgHis Leu Arg Ser Lys Val Thr Asn Phe Phe Val Ile Ser 50 55 60 Leu Ala ValSer Asp Leu Leu Val Ala Val Leu Val Met Pro Trp Lys 65 70 75 80 Ala ValAla Glu Ile Ala Gly Phe Trp Pro Phe Gly Ser Phe Cys Asn 85 90 95 Ile TrpVal Ala Phe Asp Ile Met Cys Ser Thr Ala Ser Ile Leu Asn 100 105 110 LeuCys Val Ile Ser Val Asp Arg Tyr Trp Ala Ile Ser Ser Pro Phe 115 120 125Gln Tyr Glu Arg Lys Met Thr Pro Lys Ala Ala Phe Ile Leu Ile Ser 130 135140 Val Ala Trp Thr Leu Ser Val Leu Ile Ser Phe Ile Pro Val Gln Leu 145150 155 160 Ser Trp His Lys Ala Lys Pro Thr Trp Pro Leu Asp Gly Asn PheThr 165 170 175 Ser Leu Glu Asp Thr Glu Asp Asp Asn Cys Asp Thr Arg LeuSer Arg 180 185 190 Thr Tyr Ala Ile Ser Ser Ser Leu Ile Ser Phe Tyr IlePro Val Ala 195 200 205 Ile Met Ile Val Thr Tyr Thr Ser Ile Tyr Arg IleAla Gln Lys Gln 210 215 220 Ile Arg Arg Ile Ser Ala Leu Glu Arg Ala AlaVal His Ala Lys Asn 225 230 235 240 Cys Gln Thr Thr Ala Gly Asn Gly AsnPro Val Glu Cys Ala Gln Ser 245 250 255 Glu Ser Ser Phe Lys Met Ser PheLys Arg Glu Thr Lys Val Leu Lys 260 265 270 Thr Leu Ser Val Ile Met GlyVal Phe Val Cys Cys Trp Leu Pro Phe 275 280 285 Phe Ile Ser Asn Cys MetVal Pro Phe Cys Gly Ser Glu Glu Thr Gln 290 295 300 Pro Phe Cys Ile AspSer Ile Thr Phe Asp Val Phe Val Trp Phe Gly 305 310 315 320 Trp Ala AsnSer Ser Leu Asn Pro Ile Ile Tyr Ala Phe Asn Ala Asp 325 330 335 Phe GlnLys Ala Phe Ser Thr Leu Leu Gly Cys Tyr Arg Leu Cys Pro 340 345 350 ThrThr Asn Asn Ala Ile Glu Thr Val Ser Ile Asn Asn Asn Gly Ala 355 360 365Val Val Phe Ser Ser His His Glu Pro Arg Gly Ser Ile Ser Lys Asp 370 375380 Cys Asn Leu Val Tyr Leu Ile Pro His Ala Val Gly Ser Ser Glu Asp 385390 395 400 Leu Lys Lys Glu Glu Ala Gly Gly Ile Ala Lys Pro Leu Glu LysLeu 405 410 415 Ser Pro Ala Leu Ser Val Ile Leu Asp Tyr Asp Thr Asp ValSer Leu 420 425 430 Glu Lys Ile Gln Pro Val Thr His Ser Gly Gln His SerThr 435 440 445 5 960 PRT Mus musculus GABA-B1a receptor 5 Met Leu LeuLeu Leu Leu Leu Leu Leu Phe Leu Arg Pro Leu Gly Ala 1 5 10 15 Gly GlyAla Gln Thr Pro Asn Val Thr Ser Glu Gly Cys Gln Ile Ile 20 25 30 His ProPro Trp Glu Gly Gly Ile Arg Tyr Arg Gly Leu Ile Arg Asp 35 40 45 Gln ValLys Ala Ile Asn Phe Leu Pro Val Asp Tyr Glu Ile Glu Tyr 50 55 60 Val CysArg Gly Glu Arg Glu Val Val Gly Pro Lys Val Arg Lys Cys 65 70 75 80 LeuAla Asn Gly Ser Trp Thr Asp Met Asp Thr Pro Ser Arg Cys Val 85 90 95 ArgIle Cys Ser Lys Ser Tyr Leu Thr Leu Glu Asn Gly Lys Val Phe 100 105 110Leu Thr Gly Gly Asp Leu Pro Ala Leu Asp Gly Ala Arg Val Asp Phe 115 120125 Arg Cys Asp Pro Asp Phe His Leu Val Gly Ser Ser Arg Ser Ile Cys 130135 140 Ser Gln Gly Gln Trp Ser Thr Pro Lys Pro His Cys Gln Val Asn Arg145 150 155 160 Thr Pro His Ser Glu Arg Arg Ala Val Tyr Ile Gly Ala LeuPhe Pro 165 170 175 Met Ser Gly Gly Trp Pro Gly Gly Gln Ala Cys Gln ProAla Val Glu 180 185 190 Met Ala Leu Glu Asp Val Asn Ser Arg Arg Asp IleLeu Pro Asp Tyr 195 200 205 Glu Leu Lys Leu Ile His His Asp Ser Lys CysAsp Pro Gly Gln Ala 210 215 220 Thr Lys Tyr Leu Tyr Glu Leu Leu Tyr AsnAsp Pro Ile Lys Ile Ile 225 230 235 240 Leu Met Pro Gly Cys Ser Ser ValSer Thr Leu Val Ala Glu Ala Ala 245 250 255 Arg Met Trp Asn Leu Ile ValLeu Ser Tyr Gly Ser Ser Ser Pro Ala 260 265 270 Leu Ser Asn Arg Gln ArgPhe Pro Thr Phe Phe Arg Thr His Pro Ser 275 280 285 Ala Thr Leu His AsnPro Thr Arg Val Lys Leu Phe Glu Lys Trp Gly 290 295 300 Trp Lys Lys IleAla Thr Ile Gln Gln Thr Thr Glu Val Phe Thr Ser 305 310 315 320 Thr LeuAsp Asp Leu Glu Glu Arg Val Lys Glu Ala Gly Ile Glu Ile 325 330 335 ThrPhe Arg Gln Ser Phe Phe Ser Asp Pro Ala Val Pro Val Lys Asn 340 345 350Leu Lys Arg Gln Asp Ala Arg Ile Ile Val Gly Leu Phe Tyr Glu Thr 355 360365 Glu Ala Arg Lys Val Phe Cys Glu Val Tyr Lys Glu Arg Leu Phe Gly 370375 380 Lys Lys Tyr Val Trp Phe Leu Ile Gly Trp Tyr Ala Asp Asn Trp Phe385 390 395 400 Lys Thr Tyr Asp Pro Ser Ile Asn Cys Thr Val Glu Glu MetThr Glu 405 410 415 Ala Val Glu Gly His Ile Thr Thr Glu Ile Val Met LeuAsn Pro Ala 420 425 430 Asn Thr Arg Ser Ile Ser Asn Met Thr Ser Gln GluPhe Val Glu Lys 435 440 445 Leu Thr Lys Arg Leu Lys Arg His Pro Glu GluThr Gly Gly Phe Gln 450 455 460 Glu Ala Pro Leu Ala Tyr Asp Ala Ile TrpAla Leu Ala Leu Ala Leu 465 470 475 480 Asn Lys Thr Ser Gly Gly Gly GlyArg Ser Gly Val Arg Leu Glu Asp 485 490 495 Phe Asn Tyr Asn Asn Gln ThrIle Thr Asp Gln Ile Tyr Arg Ala Met 500 505 510 Asn Ser Ser Ser Phe GluGly Val Ser Gly His Val Val Phe Asp Ala 515 520 525 Ser Gly Ser Arg MetAla Trp Thr Leu Ile Glu Gln Leu Gln Gly Gly 530 535 540 Ser Tyr Lys LysIle Gly Tyr Tyr Asp Ser Thr Lys Asp Asp Leu Ser 545 550 555 560 Trp SerLys Thr Asp Lys Trp Ile Gly Gly Ser Pro Pro Ala Asp Gln 565 570 575 ThrLeu Val Ile Lys Thr Phe Arg Phe Leu Ser Gln Lys Leu Phe Ile 580 585 590Ser Val Ser Val Leu Ser Ser Leu Gly Ile Val Leu Ala Val Val Cys 595 600605 Leu Ser Phe Asn Ile Tyr Asn Ser His Ala Arg Tyr Ile Gln Asn Ser 610615 620 Gln Pro Asn Leu Asn Asn Leu Thr Ala Val Gly Cys Ser Leu Ala Leu625 630 635 640 Ala Val Val Phe Pro Leu Gly Leu Asp Gly Tyr His Ile GlyArg Ser 645 650 655 Gln Phe Pro Phe Val Cys Gln Ala Arg Leu Trp Leu LeuGly Leu Gly 660 665 670 Phe Ser Leu Gly Tyr Gly Ser Met Phe Thr Lys IleTrp Trp Val His 675 680 685 Thr Val Phe Thr Lys Lys Glu Glu Lys Lys GluTrp Arg Lys Thr Leu 690 695 700 Glu Pro Trp Lys Leu Tyr Ala Thr Val GlyLeu Leu Val Gly Met Asp 705 710 715 720 Val Leu Thr Leu Ala Ile Trp GlnIle Val Asp Pro Leu His Arg Thr 725 730 735 Ile Glu Thr Phe Ala Lys GluGlu Pro Lys Glu Asp Ile Asp Val Ser 740 745 750 Ile Leu Pro Gln Leu GluHis Cys Ser Ser Lys Lys Met Asn Thr Trp 755 760 765 Leu Gly Ile Phe TyrGly Tyr Lys Gly Leu Leu Leu Leu Leu Gly Ile 770 775 780 Phe Leu Ala TyrGlu Thr Lys Ser Val Ser Thr Glu Lys Ile Asn Asp 785 790 795 800 His ArgAla Val Gly Met Ala Ile Tyr Asn Val Ala Val Leu Cys Leu 805 810 815 IleThr Ala Pro Val Thr Met Ile Leu Ser Ser Gln Gln Asp Ala Ala 820 825 830Phe Ala Phe Ala Ser Leu Ala Ile Val Phe Ser Ser Tyr Ile Thr Leu 835 840845 Val Val Leu Phe Val Pro Lys Met Arg Arg Leu Ile Thr Arg Gly Glu 850855 860 Trp Gln Ser Glu Thr Gln Asp Thr Met Lys Thr Gly Ser Ser Thr Asn865 870 875 880 Asn Asn Glu Glu Glu Lys Ser Arg Leu Leu Glu Lys Glu AsnArg Glu 885 890 895 Leu Glu Lys Ile Ile Ala Glu Lys Glu Glu Arg Val SerGlu Leu Arg 900 905 910 His Gln Leu Gln Ser Arg Gln Gln Leu Arg Ser ArgArg His Pro Pro 915 920 925 Thr Pro Pro Asp Pro Ser Gly Gly Leu Pro ArgGly Pro Ser Glu Pro 930 935 940 Pro Asp Arg Leu Ser Cys Asp Gly Ser ArgVal His Leu Leu Tyr Lys 945 950 955 960 6 4365 DNA Mus musculus GABA-B1areceptor 6 taacttgcgg ccgccgtcgc cccggggaag gagagacggg ggtggggtttgcgggtagcg 60 atcgcgaagg ggagagaccc cggccgggct ggagcctgga ttcctgtggaagaagaacag 120 ggggagggga agctggagga ccgggaggga gaacggggag ccgcggccgggcctgggccc 180 ttgaggcccg gggagagccg cggagcggga ccggccgccg agatgctgctgctgctgctt 240 ctgcttctct tcctccgccc cctgggcgct ggcggggctc agacccccaacgtcacctcg 300 gaaggttgcc agattataca tccgccctgg gaaggtggca tcaggtaccgtggcttgatt 360 cgcgaccagg tgaaggccat caatttcctg cctgtggact atgagattgaatatgtgtgc 420 cggggcgaac gcgaggtggt ggggcccaag gtgcgcaagt gcctggccaacggctcctgg 480 acggatatgg acacacccag tcgctgtgtc cgaatctgct ccaagtcttatttgaccctg 540 gaaaatggga aggttttcct gacgggtggg gacctcccag ctctggatggagcccgggtg 600 gatttccgat gtgaccctga cttccatctg gtgggcagct cccggagcatctgtagtcag 660 ggccagtgga gcacccccaa gccccactgc caggtgaatc gaacgccacactcagaacgg 720 cgtgcagtat acatcggggc gctgtttccc atgagcgggg gctggccggggggccaggcc 780 tgccagcctg cggtggagat ggcgctggag gacgttaaca gccgcagagacatcctgccg 840 gactacgagc tcaagcttat ccaccacgac agcaagtgcg acccagggcaagccaccaag 900 tacttgtatg aactactcta caacgacccc atcaagatca tcctcatgcccggctgcagc 960 tctgtgtcca cactggtagc cgaggctgcc cggatgtgga accttattgtgctctcatat 1020 ggctccagct caccagcctt gtcaaaccga cagcggtttc caacgttctttcggacacat 1080 ccatccgcca cactccacaa tcccacccgg gtgaaactct tcgaaaagtggggctggaag 1140 aagattgcca ccatccagca gactaccgag gtcttcacct caacactggatgacctggag 1200 gagcgagtga aagaggctgg gattgagatc acttttcgac agagtttcttctcagatcca 1260 gctgtgcctg ttaaaaacct gaagcgtcaa gatgctcgaa tcatcgtgggacttttctat 1320 gagaccgaag cccggaaagt tttttgtgag gtctataagg aacggctctttgggaagaag 1380 tatgtctggt ttctcatcgg gtggtatgct gacaactggt tcaaaacctatgacccgtca 1440 atcaattgta cagtagaaga gatgactgag gcggtggagg gccatatcaccacggagatt 1500 gtcatgctga accctgccaa cacccgaagc atttccaaca tgacatcacaggaatttgtg 1560 gagaaactaa ccaagcggct gaaaagacac cctgaggaga ctggaggcttccaggaggca 1620 ccactggcct atgatgctat ttgggccttg gctttggcct tgaacaagacctctggagga 1680 ggtggccgtt caggagtgcg cctggaggac tttaactaca acaaccagaccattacagac 1740 caaatctacc gggccatgaa ctcctcctcc tttgagggtg tttctggccacgtggtcttt 1800 gatgccagcg gctcccggat ggcatggacg cttatcgagc agctacagggcggcagctac 1860 aagaagatcg gctactacga cagcaccaag gatgatcttt cctggtccaaaacagacaag 1920 tggatcggag ggtctccccc agccgaccag accttggtca tcaagacattccgtttcctg 1980 tcacagaaac tctttatctc cgtctcagtt ctctccagcc tgggcattgttcttgctgtt 2040 gtctgtctgt cctttaacat ctacaactcc cacgctcgtt atatccagaattcccagccc 2100 aacctgaaca atctgactgc tgtgggctgc tcactggcac tggctgttgtcttccctctc 2160 gggctggatg gttaccacat agggagaagc cagttcccgt ttgtctgccaggcccgcctt 2220 tggctcttgg gcttgggctt tagtctgggc tatggctcta tgttcaccaagatctggtgg 2280 gtccacacag tcttcacgaa gaaggaggag aagaaggagt ggaggaagaccctagagccc 2340 tggaaactct atgccactgt gggcctgctg gtgggcatgg atgtcctgactcttgccatc 2400 tggcagattg tggacccctt gcaccgaacc attgagactt ttgccaaggaggaaccaaag 2460 gaagacatcg atgtctccat tctgccccag ttggagcact gcagctccaagaagatgaat 2520 acgtggcttg gcattttcta tggttacaag gggctgctgc tgctgctgggaatctttctt 2580 gcttacgaaa ccaagagcgt gtccactgaa aagatcaatg accacagggccgtgggcatg 2640 gctatctaca atgtcgcggt cctgtgtctc atcactgctc ctgtgaccatgatcctttcc 2700 agtcagcagg acgcagcctt tgcctttgcc tctctggcca tcgtgttctcttcctacatc 2760 actctggttg tgctctttgt gcccaagatg cgcaggctga tcacccgaggggaatggcag 2820 tctgaaacgc aggacaccat gaaaacagga tcatccacca acaacaacgaggaagagaag 2880 tcccgactgt tggagaagga aaaccgagaa ctggaaaaga tcatcgctgagaaagaggag 2940 cgcgtctctg aactgcgcca tcagctccag tctcggcagc aactccgctcacggcgccac 3000 cccccaacac ccccagatcc ctctgggggc cttcccaggg gaccctctgagccccctgac 3060 cggcttagct gtgatgggag tcgagtacat ttgctttaca agtgagggggcatggagaag 3120 gatcaagcca gtaggggagg gaagggtctg ggaagagggt gggggcctgggaggagggta 3180 aggactccta tctccaacct ggagagcaca cgctccaatc cccctcttataaatacatgt 3240 cgctctgtgc atctggggtt atttgggtct ccagtactct gggaaacagactgttttctt 3300 tctcccctat aattttatat ctccacttca caggttttgt ttgaaccctgcttggagtta 3360 ttattcactc atggctccag aggggcatct catttttctc cggtagcctgtcttgtacag 3420 ttaccacagc aactcctgtc atttcagcag caggggtctt cctacactagcagggctctc 3480 gctctctcca tttttcagcc tcagaatctc cttccattat tcttctccttctacatgtct 3540 ccatggcttc ctctcccagg ggactcgttc tacacacata cacacacacacacacacaca 3600 cacacacaca cacacacaca cacaccccgc atcctgccct ctcctaggcagctgcatgtc 3660 gtcctgtaca aatgtgctcg cttctgagtg ctttgtgcgg ccgttcacttgtgctgtctg 3720 cataagctgc gtctgtgagt gcacggtggt ttgtgggtgc gtgaagtggcatgctccggt 3780 aggtgtgtgt gtgtgtgccc acgcgtgcgc ccgtgtgcat gcgttcgtgttgccctgact 3840 ggctgtctca gccttctgag taattgggat tccagttgtc tgtctagctcatgtcctgtc 3900 ttcttccagt agagccgtga acacccaaca cacacagtta atcgggctccccccagtcca 3960 tgttttctga gccatccaaa aactctcctt ggccttaggt tcatctgcaaatgttccctc 4020 tgttctttgc tctcgtgcgt ccaccttcat tctcttcagt catttctcagatctgctgcg 4080 tcgtggtttc ctttccttca ttatcatcgt cattattttt cagaacttaagggaaaaaga 4140 aatggggaca ggttggaggc tgtttccagt ggaatagtgg gtgcgcgtcctgaccaaatg 4200 aaggcacgga cagatggact gacggggcgg gaggcggcgt ccctttcacaccgtggtgtc 4260 tcttgggggg gaaggatctc cctgaatctc aataaagcag tgaacagtaaaaaaaaaaaa 4320 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa 4365 7582 PRT Rattus norvegicus Differentiation-associated Na-dependentinorganic phosphate cotransporter 7 Met Glu Ser Val Lys Gln Arg Ile LeuAla Pro Gly Lys Glu Gly Ile 1 5 10 15 Lys Asn Phe Ala Gly Lys Ser LeuGly Gln Ile Tyr Arg Val Leu Glu 20 25 30 Lys Lys Gln Asp Asn Arg Glu ThrIle Glu Leu Thr Glu Asp Gly Lys 35 40 45 Pro Leu Glu Val Pro Glu Lys LysAla Pro Leu Cys Asp Cys Thr Cys 50 55 60 Phe Gly Leu Pro Arg Arg Tyr IleIle Ala Ile Met Ser Gly Leu Gly 65 70 75 80 Phe Cys Ile Ser Phe Gly IleArg Cys Asn Leu Gly Val Ala Ile Val 85 90 95 Asp Met Val Asn Asn Ser ThrIle His Arg Gly Gly Lys Val Ile Lys 100 105 110 Glu Lys Ala Lys Phe AsnTrp Asp Pro Glu Thr Val Gly Met Ile His 115 120 125 Gly Ser Phe Phe TrpGly Tyr Ile Ile Thr Gln Ile Pro Gly Gly Tyr 130 135 140 Ile Ala Ser ArgLeu Ala Ala Asn Arg Val Phe Gly Ala Ala Ile Leu 145 150 155 160 Leu ThrSer Thr Leu Asn Met Leu Ile Pro Ser Ala Ala Arg Val His 165 170 175 TyrGly Cys Val Ile Phe Val Arg Ile Leu Gln Gly Leu Val Glu Gly 180 185 190Val Thr Tyr Pro Ala Cys His Gly Ile Trp Ser Lys Trp Ala Pro Pro 195 200205 Leu Glu Arg Ser Arg Leu Ala Thr Thr Ser Phe Cys Gly Ser Tyr Ala 210215 220 Gly Ala Val Ile Ala Met Pro Leu Ala Gly Ile Leu Val Gln Tyr Thr225 230 235 240 Gly Trp Ser Ser Val Phe Tyr Val Tyr Gly Ser Phe Gly MetVal Trp 245 250 255 Tyr Met Phe Trp Leu Leu Val Ser Tyr Glu Ser Pro AlaLys His Pro 260 265 270 Thr Ile Thr Asp Glu Glu Arg Arg Tyr Ile Glu GluSer Ile Gly Glu 275 280 285 Ser Ala Asn Leu Leu Gly Ala Met Glu Lys PheLys Thr Pro Trp Arg 290 295 300 Lys Phe Phe Thr Ser Met Pro Val Tyr AlaIle Ile Val Ala Asn Phe 305 310 315 320 Cys Arg Ser Trp Thr Phe Tyr LeuLeu Leu Ile Ser Gln Pro Ala Tyr 325 330 335 Phe Glu Glu Val Phe Gly PheGlu Ile Ser Lys Val Gly Met Leu Ser 340 345 350 Ala Val Pro His Leu ValMet Thr Ile Ile Val Pro Ile Gly Gly Gln 355 360 365 Ile Ala Asp Phe LeuArg Ser Lys Gln Ile Leu Ser Thr Thr Thr Val 370 375 380 Arg Lys Ile MetAsn Cys Gly Gly Phe Gly Met Glu Ala Thr Leu Leu 385 390 395 400 Leu ValVal Gly Tyr Ser His Thr Arg Gly Val Ala Ile Ser Phe Leu 405 410 415 ValLeu Ala Val Gly Phe Ser Gly Phe Ala Ile Ser Gly Phe Asn Val 420 425 430Asn His Leu Asp Ile Ala Pro Arg Tyr Ala Ser Ile Leu Met Gly Ile 435 440445 Ser Asn Gly Val Gly Thr Leu Ser Gly Met Val Cys Pro Ile Ile Val 450455 460 Gly Ala Met Thr Lys Asn Lys Ser Arg Glu Glu Trp Gln Tyr Val Phe465 470 475 480 Leu Ile Ala Ala Leu Val His Tyr Gly Gly Val Ile Phe TyrAla Leu 485 490 495 Phe Ala Ser Gly Glu Lys Gln Pro Trp Ala Asp Pro GluGlu Thr Ser 500 505 510 Glu Glu Lys Cys Gly Phe Ile His Glu Asp Glu LeuAsp Glu Glu Thr 515 520 525 Gly Asp Ile Thr Gln Asn Tyr Ile Asn Tyr GlyThr Thr Lys Ser Tyr 530 535 540 Gly Ala Thr Ser Gln Glu Asn Gly Gly TrpPro Asn Gly Trp Glu Lys 545 550 555 560 Lys Glu Glu Phe Val Gln Glu SerAla Gln Asp Ala Tyr Ser Tyr Lys 565 570 575 Asp Arg Asp Asp Tyr Ser 5808 3982 DNA Rattus norvegicus Differentiation-associated Na-dependentinorganic phosphate cotransporter 8 agacagtaag gttcttttgc ttttttcccttacacaagga ttcgatgacg tttttggtca 60 atctgattaa aagacagcgg atttggttgcgttaagactt caaaaccggg aatttacgtt 120 gtttttcggt gaggtgactt ccagaacggggactcatcag cacccgccca aataccacgg 180 cactgcgcgc gccctcggcc accggatcctccccttccaa tgagactttg tgactgtgtg 240 taccaattct cctattagga aacccgtgggctgaatgcag ctattccgtt gtactctctt 300 tctcgctctc cctcccctct ccaactcacagccttgctga aaagctcatc tctgctgaga 360 agaaaacgtt ctaccttaac ctattaagactatgcgcaga actcgccttt catagccatc 420 acaatttaaa tctggtaagg ctggacacgagtctttacaa gaatggagtc ggtaaaacaa 480 aggattttgg ccccggggaa agaggggataaagaattttg ctggaaaatc cctcggacag 540 atctacaggg tgctggagaa gaagcaggataaccgagaga ccatcgagct gacagaggac 600 ggcaagcccc tggaggtgcc tgagaagaaggctccgctat gcgactgtac gtgcttcggc 660 ctgccgcgcc gctacatcat agccatcatgagcggcctcg gcttctgcat ctcctttggt 720 atccgctgta acctgggtgt ggccattgtggacatggtca acaacagcac catccaccgg 780 ggtggcaaag ttatcaagga gaaagccaagtttaactggg accccgagac tgtggggatg 840 attcacgggt cgttcttctg gggctatatcatcacgcaga ttccgggcgg atacatcgca 900 tcgcgactgg ctgctaaccg ggtctttggggctgccatac tgcttacctc taccctcaat 960 atgctgatcc catctgcagc cagagtgcattatggatgcg tcatctttgt tagaatattg 1020 caaggacttg tggagggcgt cacctacccagcctgtcacg ggatatggag caagtgggcc 1080 cctcctttgg agaggagtag gttggctaccacctccttct gtggttccta tgctggagca 1140 gtcattgcaa tgcccctagc tggtatcctggtgcagtaca ctggatggtc ttcagtattt 1200 tacgtatatg gaagctttgg tatggtctggtatatgttct ggcttctggt gtcttacgag 1260 agccccgcaa agcatccaac cataacagacgaagaacgta ggtacataga agagagcatc 1320 ggggagagcg caaatctgtt aggagcaatggagaaattca agaccccatg gaggaagttt 1380 ttcacatcca tgcccgtcta tgcgataattgttgcaaact tctgcaggag ttggactttt 1440 tatttactgc tcatcagtca accagcttatttcgaggagg tttttggatt tgaaatcagc 1500 aaggttggca tgttgtctgc ggtcccacacctggtcatga caatcattgt gcctatcggg 1560 gggcaaattg cagactttct aaggagcaagcaaattcttt caacaactac agtgcgaaag 1620 atcatgaact gcgggggttt tggcatggaagccacactgc ttctggttgt tggctactct 1680 catactagag gggtggccat ctccttcttggtgcttgcag tgggattcag tggatttgct 1740 atctctggtt tcaatgtgaa ccacttggatattgccccga gatatgccag tatcttaatg 1800 ggcatttcaa atggtgttgg cacgctgtcgggaatggtct gcccgatcat tgttggtgca 1860 atgacgaaga acaagtcccg tgaagaatggcagtatgtct tcctcatcgc tgcactggtc 1920 cactatggtg gagtcatatt ttatgcactatttgcctcag gagagaagca accttgggca 1980 gaccctgagg aaacaagcga agaaaagtgtggcttcattc atgaagatga actggatgaa 2040 gaaacggggg acatcactca gaattacataaattacggta ccaccaaatc ctacggcgcc 2100 acctcacagg agaacggagg ctggcctaacggctgggaga aaaaggaaga atttgtgcaa 2160 gaaagtgcgc aagacgcgta ctcctataaggaccgagatg attattcata acgaagctag 2220 ttgctggatt cctttgtagt gtttgtgattaaattaattg tgattgcaca aaatcatttt 2280 aagaaatgtg gtgtaaacat gtaaacacatcaaccaagca agtcttgctg ttcaaaaaat 2340 aataataata tgaattcaaa acagaccgtgagagtcccat caagtgcaat ctgtggcggc 2400 agtcacgtga cgccatttcc attcaggccattcgtccttt tcgtttgtga tttaaaggtt 2460 tcctgtagaa ataagtaggt attcgttggatccatcacca cgttagagag tacaactaca 2520 acagttggca catgtcatcc tacggaagttaggaagccaa agctactgga ttatgtgaac 2580 tgcatttatt tatttattac actggactgcaaaatatccc agggaaatcc tgtctagaga 2640 catagtagaa ctggaaagat ggctagattgggtactgacg ataatcattg tgtgtatatc 2700 atggagtggc tatatctttt aattggagaactatattgta tagctagcaa aattgtactg 2760 aattattact aggagtgcac agtgtgtgatattttgtgat cttccaaaag cttatcttgc 2820 agtgttttgt gaaacgcttg ggcacaaacacttattttta tgaacaagag cttgtaaagg 2880 gaggagtatg ctccatgctc tcccattcactacctgacag tatcaaacct tcacatttca 2940 atgaaatcca acgtccatgt aacatatcacatgacttttt ttgcaaaaaa gaatataaga 3000 agaaatagac ttcaatgtat tttttattacaactttgtac tggttgtaac ttgcattagg 3060 aaaaatgatt aatatatgta taatcgtaaagaatctaata aaaatttact atgaagatat 3120 agcccttaaa atgcaatatt aacaacaaaaatatatagaa aatttagata atcttccttg 3180 ataactagag actatatgga actcacaccacaaagctata tataatatga aaagataaac 3240 aatagagatt gtatatgtag acgattttatgacctaatgt cccatttaag aggtatttgt 3300 cttgagtata tagtacaaag tatattaaaattatatctac atccctgtat atcttataca 3360 tatccactca cacaaacata acaaatacttttcacacaga accaaaaaca agcatacacc 3420 taatgttggg tttggggatt gcaatttctactttcataga gtcatagaat tttagatggg 3480 aaaaaaaaag gcattttgct cgtcatttcttaatataatt aattcaacag gaactgcaac 3540 atttgtgtac caagcaataa gtgcgaagcataaacctgct gtgtgtaaac tatccccata 3600 ctgcttgtgg tagcactgat ttctttcttttaaagaactt aacatcggag ctctttacaa 3660 tgttttgcgc tgataagaat gcacatcccaatttaacgca aagtgtcacc tggtgtgttt 3720 acctgtctgt tttgggtatt tggtctgtttggtgtcctgt gctcttgact ggaggccctg 3780 ctactgcgaa tataaaacgt gaagtttgtttctaaatgca aaccactcct gaccttaaga 3840 aactaaagtc cctctctgct ttgtgtctccaagtactatc atgtgaccat aacccttgct 3900 gtgctgagta aaaagatgtg aactgtcattttgttgctgc gaagcaagtg ttaataaaat 3960 gttctattta aaaaaaaaaa aa 3982 9854 PRT Homo sapiens Vacuolar assembly protein VSP41 gene 9 Met Ala GluAla Val Glu Gln Glu Thr Gly Ser Leu Glu Glu Ser Thr 1 5 10 15 Asp GluSer Glu Glu Glu Glu Ser Glu Glu Glu Pro Lys Leu Lys Tyr 20 25 30 Glu ArgLeu Ser Asn Gly Val Thr Glu Ile Leu Gln Lys Asp Ala Ala 35 40 45 Ser CysMet Thr Val His Asp Lys Phe Leu Ala Leu Gly Thr His Tyr 50 55 60 Gly LysVal Tyr Leu Leu Asp Val Gln Gly Asn Ile Thr Gln Lys Phe 65 70 75 80 AspVal Ser Pro Val Lys Ile Asn Gln Ile Ser Leu Asp Glu Ser Gly 85 90 95 GluHis Met Gly Val Cys Ser Glu Asp Gly Lys Val Gln Val Phe Gly 100 105 110Leu Tyr Ser Gly Glu Glu Phe His Glu Thr Phe Asp Cys Pro Ile Lys 115 120125 Ile Ile Ala Val His Pro His Phe Val Arg Ser Ser Cys Lys Gln Phe 130135 140 Val Thr Gly Gly Lys Lys Leu Leu Leu Phe Glu Arg Ser Trp Met Asn145 150 155 160 Arg Trp Lys Ser Ala Val Leu His Glu Gly Glu Gly Asn IleArg Ser 165 170 175 Val Lys Trp Arg Gly His Leu Ile Ala Trp Ala Asn AsnMet Gly Val 180 185 190 Lys Ile Phe Asp Ile Ile Ser Lys Gln Arg Ile ThrAsn Val Pro Arg 195 200 205 Asp Asp Ile Ser Leu Arg Pro Asp Met Tyr ProCys Ser Leu Cys Trp 210 215 220 Lys Asp Asn Val Thr Leu Ile Ile Gly TrpGly Thr Ser Val Lys Val 225 230 235 240 Cys Ser Val Lys Glu Arg His AlaSer Glu Met Arg Asp Leu Pro Ser 245 250 255 Arg Tyr Val Glu Ile Val SerGln Phe Glu Thr Glu Phe Tyr Ile Ser 260 265 270 Gly Leu Ala Pro Leu CysAsp Gln Leu Val Val Leu Ser Tyr Val Lys 275 280 285 Glu Ile Ser Glu LysThr Glu Arg Glu Tyr Cys Ala Arg Pro Arg Leu 290 295 300 Asp Ile Ile GlnPro Leu Ser Glu Thr Cys Glu Glu Ile Ser Ser Asp 305 310 315 320 Ala LeuThr Val Arg Gly Phe Gln Glu Asn Glu Cys Arg Asp Tyr His 325 330 335 LeuGlu Tyr Ser Glu Gly Glu Ser Leu Phe Tyr Ile Val Ser Pro Arg 340 345 350Asp Val Val Val Ala Lys Glu Arg Asp Gln Asp Asp His Ile Asp Trp 355 360365 Leu Leu Glu Lys Lys Lys Tyr Glu Glu Ala Leu Met Ala Ala Glu Ile 370375 380 Ser Gln Lys Asn Ile Lys Arg His Lys Ile Leu Asp Ile Gly Leu Ala385 390 395 400 Tyr Ile Asn His Leu Val Glu Arg Gly Asp Tyr Asp Ile AlaAla Arg 405 410 415 Lys Cys Gln Lys Ile Leu Gly Lys Asn Ala Ala Leu TrpGlu Tyr Glu 420 425 430 Val Tyr Lys Phe Lys Glu Ile Gly Gln Leu Lys AlaIle Ser Pro Tyr 435 440 445 Leu Pro Arg Gly Asp Pro Val Leu Lys Pro LeuIle Tyr Glu Met Ile 450 455 460 Leu His Glu Phe Leu Glu Ser Asp Tyr GluGly Phe Ala Thr Leu Ile 465 470 475 480 Arg Glu Trp Pro Gly Asp Leu TyrAsn Asn Ser Val Ile Val Gln Ala 485 490 495 Val Arg Asp His Leu Lys LysAsp Ser Gln Asn Lys Thr Leu Leu Lys 500 505 510 Thr Leu Ala Glu Leu TyrThr Tyr Asp Lys Asn Tyr Gly Asn Ala Leu 515 520 525 Glu Ile Tyr Leu ThrLeu Arg His Lys Asp Val Phe Gln Leu Ile His 530 535 540 Lys His Asn LeuPhe Ser Ser Ile Lys Asp Lys Ile Val Leu Leu Met 545 550 555 560 Asp PheAsp Ser Glu Lys Ala Val Asp Met Leu Leu Asp Asn Glu Asp 565 570 575 LysIle Ser Ile Lys Lys Val Val Glu Glu Leu Glu Asp Arg Pro Glu 580 585 590Leu Gln His Val Tyr Leu His Lys Leu Phe Lys Arg Asp His His Lys 595 600605 Gly Gln Arg Tyr His Glu Lys Gln Ile Ser Leu Tyr Ala Glu Tyr Asp 610615 620 Arg Pro Asn Leu Leu Pro Phe Leu Arg Asp Ser Thr His Cys Pro Leu625 630 635 640 Glu Lys Ala Leu Glu Ile Cys Gln Gln Arg Asn Phe Val GluGlu Thr 645 650 655 Val Tyr Leu Leu Ser Arg Met Gly Asn Ser Arg Ser AlaLeu Lys Met 660 665 670 Ile Met Glu Glu Leu His Asp Val Asp Lys Ala IleGlu Phe Ala Lys 675 680 685 Glu Gln Asp Asp Gly Glu Leu Trp Glu Asp LeuIle Leu Tyr Ser Ile 690 695 700 Asp Lys Pro Pro Phe Ile Thr Gly Leu LeuAsn Asn Ile Gly Thr His 705 710 715 720 Val Asp Pro Ile Leu Leu Ile HisArg Ile Lys Glu Gly Met Glu Ile 725 730 735 Pro Asn Leu Arg Asp Ser LeuVal Lys Ile Leu Gln Asp Tyr Asn Leu 740 745 750 Gln Ile Leu Leu Arg GluGly Cys Lys Lys Ile Leu Val Ala Asp Ser 755 760 765 Leu Ser Leu Leu LysLys Met His Arg Thr Gln Met Lys Gly Val Leu 770 775 780 Val Asp Glu GluAsn Ile Cys Glu Ser Cys Leu Ser Pro Ile Leu Pro 785 790 795 800 Ser AspAla Ala Lys Pro Phe Ser Val Val Val Phe His Cys Arg His 805 810 815 MetPhe His Lys Glu Cys Leu Pro Met Pro Ser Met Asn Ser Ala Ala 820 825 830Gln Phe Cys Asn Ile Cys Ser Ala Lys Asn Arg Gly Pro Gly Ser Ala 835 840845 Ile Leu Glu Met Lys Lys 850 10 770 PRT Rattus norvegicus Git 1 10Met Ser Arg Lys Gly Pro Arg Ala Glu Val Cys Ala Asp Cys Ser Ala 1 5 1015 Pro Asp Pro Gly Trp Ala Ser Ile Ser Arg Gly Val Leu Val Cys Asp 20 2530 Glu Cys Cys Ser Val His Arg Ser Leu Gly Arg His Ile Ser Ile Val 35 4045 Lys His Leu Arg His Ser Ala Trp Pro Pro Thr Leu Leu Gln Met Val 50 5560 His Thr Leu Ala Ser Asn Gly Ala Asn Ser Ile Trp Glu His Ser Leu 65 7075 80 Leu Asp Pro Ala Gln Val Gln Ser Gly Arg Arg Lys Ala Asn Pro Gln 8590 95 Asp Lys Val His Pro Ile Lys Ser Glu Phe Ile Arg Ala Lys Tyr Gln100 105 110 Met Leu Ala Phe Val His Lys Leu Pro Cys Arg Asp Asp Asp GlyVal 115 120 125 Thr Ala Lys Asp Leu Ser Lys Gln Leu His Ser Ser Val ArgThr Gly 130 135 140 Asn Leu Glu Thr Cys Leu Arg Leu Leu Ser Leu Gly AlaGln Ala Asn 145 150 155 160 Phe Phe His Pro Glu Lys Gly Thr Thr Pro LeuHis Val Ala Ala Lys 165 170 175 Ala Gly Gln Thr Leu Gln Ala Glu Leu LeuVal Val Tyr Gly Ala Asp 180 185 190 Pro Gly Ser Pro Asp Val Asn Gly ArgThr Pro Ile Asp Tyr Ala Arg 195 200 205 Gln Ala Gly His His Glu Leu AlaGlu Arg Leu Val Glu Cys Gln Tyr 210 215 220 Glu Leu Thr Asp Arg Leu AlaPhe Tyr Leu Cys Gly Arg Lys Pro Asp 225 230 235 240 His Lys Asn Gly HisTyr Ile Ile Pro Gln Met Ala Asp Arg Ser Arg 245 250 255 Gln Lys Cys MetSer Gln Ser Leu Asp Leu Ser Glu Leu Ala Lys Ala 260 265 270 Ala Lys LysLys Leu Gln Ala Leu Ser Asn Arg Leu Phe Glu Glu Leu 275 280 285 Ala MetAsp Val Tyr Asp Glu Val Asp Arg Arg Glu Asn Asp Ala Val 290 295 300 TrpLeu Ala Thr Gln Asn His Ser Thr Leu Val Thr Glu Arg Ser Ala 305 310 315320 Val Pro Phe Leu Pro Val Asn Pro Glu Tyr Ser Ala Thr Arg Asn Gln 325330 335 Gly Arg Gln Lys Leu Ala Arg Phe Asn Ala Arg Glu Phe Ala Thr Leu340 345 350 Ile Ile Asp Ile Leu Ser Glu Ala Lys Arg Arg Gln Gln Gly LysSer 355 360 365 Leu Ser Ser Pro Thr Asp Asn Leu Glu Leu Ser Ala Arg AsnGln Ser 370 375 380 Asp Leu Asp Asp Gln His Asp Tyr Asp Ser Val Ala SerAsp Glu Asp 385 390 395 400 Thr Asp Gln Glu Pro Leu Pro Ser Ala Gly AlaThr Arg Asn Asn Arg 405 410 415 Ala Arg Ser Met Asp Ser Ser Asp Leu SerAsp Gly Ala Val Thr Leu 420 425 430 Gln Glu Tyr Leu Glu Leu Lys Lys AlaLeu Ala Thr Ser Glu Ala Lys 435 440 445 Val Gln Gln Leu Met Lys Val AsnSer Ser Leu Ser Asp Glu Leu Arg 450 455 460 Lys Leu Gln Arg Glu Ile HisLys Leu Gln Ala Glu Asn Leu Gln Leu 465 470 475 480 Arg Gln Pro Pro GlyPro Val Pro Val Pro Ser Leu Pro Ser Glu Arg 485 490 495 Ala Glu His ThrLeu Met Gly Pro Gly Gly Ser Thr His Arg Arg Asp 500 505 510 Arg Gln AlaPhe Ser Met Tyr Glu Pro Gly Ser Ala Leu Lys Pro Phe 515 520 525 Gly GlyAla Pro Gly Asp Glu Leu Ala Thr Arg Leu Gln Pro Phe His 530 535 540 SerThr Glu Leu Glu Asp Asp Ala Ile Tyr Ser Val His Val Pro Ala 545 550 555560 Gly Leu Tyr Arg Ile Arg Lys Gly Val Ser Ala Ser Ser Val Thr Phe 565570 575 Thr Pro Ser Ser Pro Leu Leu Ser Ser Ser Gln Glu Gly Ser Arg His580 585 590 Ala Ser Lys Leu Ser Arg His Gly Ser Gly Ala Glu Ser Asp TyrGlu 595 600 605 Asn Thr Gln Ser Gly Glu Pro Leu Leu Gly Leu Glu Gly LysArg Phe 610 615 620 Leu Glu Leu Ser Lys Glu Asp Glu Leu His Ala Glu LeuGlu Ser Leu 625 630 635 640 Asp Gly Asp Pro Asp Pro Gly Leu Pro Ser ThrGlu Asp Val Ile Leu 645 650 655 Lys Thr Glu Gln Val Thr Lys Asn Ile GlnGlu Leu Leu Arg Ala Ala 660 665 670 Gln Glu Phe Lys His Asp Ser Phe ValPro Cys Ser Glu Lys Ile His 675 680 685 Leu Ala Val Thr Glu Met Ala SerLeu Phe Pro Lys Arg Pro Ala Leu 690 695 700 Glu Pro Val Arg Ser Ser LeuArg Leu Leu Asn Ala Ser Ala Tyr Arg 705 710 715 720 Leu Gln Ser Glu CysArg Lys Thr Val Pro Pro Glu Pro Gly Ala Pro 725 730 735 Val Asp Phe GlnLeu Leu Thr Gln Gln Val Ile Gln Cys Ala Tyr Asp 740 745 750 Ile Ala LysAla Ala Lys Gln Leu Val Thr Ile Thr Thr Arg Glu Lys 755 760 765 Lys Gln770 11 3236 DNA Rattus norvegicus Git 1 11 gcccgcgccc cccacgctggcccagagggc tgcggccgct tcgccgctga ggatgtcccg 60 gaaggggccg cgagcggaggtgtgtgcgga ctgcagcgcc cctgaccctg gctgggcatc 120 tatcagcaga ggtgtgctggtttgtgacga gtgctgcagt gtgcaccgga gcctgggacg 180 acacatctcc attgtcaaacaccttcgcca cagcgcctgg cctcctacgc tgctacagat 240 ggtgcacacg cttgccagcaacggggccaa ctctatctgg gagcattccc tgctggaccc 300 tgcacaagtg caaagtggccggcgcaaagc caacccccaa gacaaagtcc accccatcaa 360 gtcagagttc atcagggcaaagtaccagat gctggcgttt gtgcacaagc ttccctgccg 420 tgatgatgat ggggtcaccgccaaagacct cagcaagcaa ctgcactcga gtgtgcgaac 480 aggcaacctg gagacctgtctgcgcctgct ttccctgggt gcccaggcca acttcttcca 540 cccagaaaag ggcactacacctctacacgt ggctgccaag gcagggcaga cactgcaggc 600 tgagctgcta gtagtgtatggggctgaccc aggctcccct gatgtcaatg gccgcacacc 660 cattgactat gccaggcaggcggggcacca tgaactggca gaaaggctag ttgagtgcca 720 gtatgagctc actgaccggttggccttcta cctctgtgga cggaagcctg atcacaagaa 780 tgggcattac atcataccacagatggctga cagatctcgg caaaagtgca tgtctcagag 840 tttggatctg tctgaattggccaaagctgc caagaagaag ctacaagcac tcagcaaccg 900 gctctttgag gagctcgccatggatgtgta tgacgaagtg gaccggagag aaaatgatgc 960 agtgtggcta gctacccagaatcatagcac cctggtgaca gaacgcagtg ctgtgccctt 1020 cctgccagtc aaccctgaatactcagccac tcggaatcag ggacgacaga aattggctcg 1080 ttttaatgcc cgagagtttgccaccttgat catcgacatc ctcagtgagg ccaaacggag 1140 gcagcagggc aagagtctgagcagccccac agacaacctc gagctgtctg cacggaacca 1200 aagtgacctg gacgaccagcacgactacga tagtgtggct tctgacgaag acacagacca 1260 ggagcccttg cccagtgctggcgccacgcg gaacaatcgt gccaggagca tggactcctc 1320 agatctgtcg gatggggctgtgacgctgca ggagtacctg gagctgaaga aggctctggc 1380 tacctccgag gccaaagtgcagcagctcat gaaagtcaac agcagtctga gtgacgagct 1440 tcggaagctg cagagggagatccacaaact gcaggcagag aacctgcagc tccggcagcc 1500 accaggacca gtgcctgtaccctcacttcc cagtgaacgg gcagaacaca cactcatggg 1560 ccctggtgga agtacccatcgcagggaccg ccaggccttc tctatgtatg agccaggctc 1620 cgccctgaag ccctttggaggtgcacctgg ggatgagctc gccacacggc tccagccttt 1680 ccacagcaca gagctggaagatgatgccat ctattcagta catgtccctg ctggccttta 1740 ccggatccgg aagggggtgtctgcctcttc tgtgaccttc actccctcct ccccactgct 1800 gtcaagctcc caagaaggaagtcgccatgc gagcaagctt tcacgccacg gcagtggcgc 1860 agagagtgac tatgagaacacacagagtgg agagcctctg ctgggacttg aagggaagcg 1920 attcctagag ctgagcaaggaggatgagct gcacgctgag ctggagagct tagatggaga 1980 cccagacccc gggctccccagcacagaaga tgtcatccta aagacagagc aggtcaccaa 2040 gaacattcag gagctattgcgggcagccca ggagttcaaa catgacagct ttgtgccctg 2100 ttcagaaaag atccatttggctgtgactga gatggcctct ctcttcccaa agaggccagc 2160 cctggagcct gtgcgcagttcactgcggct gctcaacgcc agcgcctacc ggctgcagag 2220 tgaatgccgg aagacagtgcctccagaacc tggcgcccct gtggacttcc agctactgac 2280 tcagcaggtg atccagtgcgcctatgacat cgccaaggct gccaagcagc tggtcaccat 2340 caccacccga gagaagaaacagtgaccact ttccctgccc ttacccacac cctgggacct 2400 cactggccat gggagctgggccactccaga cactaaccca cccccaacag agccactggc 2460 acaagtgccc ttagtgccaccattctcctc tggcatccag gtgccctggt gtccacaccc 2520 ccttaagccc caaggatgggaatgtgaggt gacaggacca tctgtccccc acattccacg 2580 cccctccctc tgtacatagcatccctgccc tgctgatacg caggagcctc aaggagtccc 2640 tcccagctct tcacctgccttccagggccc cctcagcaaa gggtgggaca gggacactcc 2700 aagtggggca gccccgcctactgcacccta tccccatgag ccagttcagc cctggggggt 2760 cgagcagagg caccccctcctttgtgcatt atctmtgggt gtytytgccc cgtagccagc 2820 agcccccccg ctgctctttcatgacaaatg gcccctgcca gagcacaggc cccaacttgt 2880 gtgccagggt ccccagcagcaaacactgga acgtctttct ctcccctacc ccttaatttt 2940 aactttgtgg taactgagtgtccctgcgtt cttacaactg agtgtgtgag cggcagtgcc 3000 gtgccagaga cctggtccacctggagctct gaggggcagg ggaaccagag tagtgggacc 3060 agcattggaa gtcagcttccctctacccaa cctgggacgg ggactgtcag ggtcaccagc 3120 tgtggtccag ttgctacccctacttgtatc cctatcctat aaacttaaaa ggaaaaccac 3180 ttccctccat ctctccccatcccctgtggg aagtgtgctg accaggcaga ggacag 3236 12 136 PRT Homo sapiensHistone H3.3 12 Met Ala Arg Thr Lys Gln Thr Ala Arg Lys Ser Thr Gly GlyLys Ala 1 5 10 15 Pro Arg Lys Gln Leu Ala Thr Lys Ala Ala Arg Lys SerAla Pro Ser 20 25 30 Thr Gly Gly Val Lys Lys Pro His Arg Tyr Arg Pro GlyThr Val Ala 35 40 45 Leu Arg Glu Ile Arg Arg Tyr Gln Lys Ser Thr Glu LeuLeu Ile Arg 50 55 60 Lys Leu Pro Phe Gln Arg Leu Val Arg Glu Ile Ala GlnAsp Phe Lys 65 70 75 80 Thr Asp Leu Arg Phe Gln Ser Ala Ala Ile Gly AlaLeu Gln Glu Ala 85 90 95 Ser Glu Ala Tyr Leu Val Gly Leu Phe Glu Asp ThrAsn Leu Cys Ala 100 105 110 Ile His Ala Lys Arg Val Thr Ile Met Pro LysAsp Ile Gln Leu Ala 115 120 125 Arg Arg Ile Arg Gly Glu Arg Ala 130 13513 1039 DNA Homo sapiens Histone H3.3 13 tgttcgcagc cgccgccgcgccgccgtcgc tctccaacgc cagcgccgcc tctcgctcgc 60 gtaagtaagg aggtctctgcgagctccagc cgaagagaag ggggtaccat ggctcgtaca 120 aagcagactg cccgcaaatcgaccggtggt aaagcaccca ggaagcaact ggctacaaaa 180 gccgctcgca agagtgcgccctctactgga ggggtgaaga aacctcatcg ttacaggcct 240 ggtactgtgg cgctccgtgaaattagacgt tatcagaagt ccactgaact tctgattcgc 300 aaacttccct tccagcgtctggtgcgagaa attgctcagg actttaaaac agatctgcgc 360 ttccagagcg cagctatcggtgctttgcag gaggcaagtg aggcctatct ggttggcctt 420 tttgaagaca ccaacctgtgtgctatccat gccaaacgtg taacaattat gccaaaagac 480 atccagctag cacgccgcatacgtggagaa cgtgcttaag aatccactat gatgggaaac 540 atttcattct caaaaaaaaaaaaaaaattt ctcttcttcc tgttattggt agttctgaac 600 gttagatatt ttttttccatggggtcaaag gtacctaagt atatgattgc gagtggaaaa 660 ataggggaca gaaatcaggtattggcagtt tttccatttt catttgtgtg tgaattttta 720 atataaatgc ggagacgtaaagcattaatg caagttaaaa tgtttcagtg aacaagtttc 780 agcggttcaa ctttataataattataaata aacctgttaa atttttctgg acaatgccag 840 catttggatt tctttaaaacaagtaaattt cttattgatg gcaactaaat ggtgtttgta 900 gcatttttat catacagtagattccatcca ttcactatac ttttctaact gagttgtcct 960 acatgcaagt acatgtttttaatgttgtct gtcttctgtg ctgttcctgt aagtttgcta 1020 ttaaaataca ttaaactat1039 14 520 PRT Rattus norvegicus Cytosolic 3-hydroxy 3-methylglutarylcoenzyme A synthase 14 Met Pro Gly Ser Leu Pro Leu Asn Ala Glu Ala CysTrp Pro Lys Asp 1 5 10 15 Val Gly Ile Val Ala Leu Glu Ile Tyr Phe ProSer Gln Tyr Val Asp 20 25 30 Gln Ala Glu Leu Glu Lys Tyr Asp Gly Val AspAla Gly Lys Tyr Thr 35 40 45 Ile Gly Leu Gly Gln Ala Arg Met Gly Phe CysThr Asp Arg Glu Asp 50 55 60 Ile Asn Ser Leu Cys Leu Thr Val Val Gln LysLeu Met Glu Arg Asn 65 70 75 80 Ser Leu Ser Tyr Asp Cys Ile Gly Arg LeuGlu Val Gly Thr Glu Thr 85 90 95 Ile Ile Asp Lys Ser Lys Ser Val Lys SerAsn Leu Met Gln Leu Phe 100 105 110 Glu Glu Ser Gly Asn Thr Asp Ile GluGly Ile Asp Thr Thr Asn Ala 115 120 125 Cys Tyr Gly Gly Thr Ala Ala ValPhe Asn Ala Val Asn Trp Ile Glu 130 135 140 Ser Ser Ser Trp Asp Gly ArgTyr Ala Leu Val Val Ala Gly Asp Ile 145 150 155 160 Ala Ile Tyr Ala SerGly Asn Ala Arg Pro Thr Gly Gly Val Gly Ala 165 170 175 Val Ala Leu LeuIle Gly Pro Asn Ala Pro Val Ile Phe Asp Arg Gly 180 185 190 Leu Arg GlyThr His Met Gln His Ala Tyr Asp Phe Tyr Lys Pro Asp 195 200 205 Met LeuSer Glu Tyr Pro Val Val Asp Gly Lys Leu Ser Ile Gln Cys 210 215 220 TyrLeu Ser Ala Leu Asp Arg Cys Tyr Ser Val Tyr Arg Lys Lys Ile 225 230 235240 Arg Ala Gln Trp Gln Lys Glu Gly Lys Asp Lys Asp Phe Thr Leu Asn 245250 255 Asp Phe Gly Phe Met Ile Phe His Ser Pro Tyr Cys Lys Leu Val Gln260 265 270 Lys Ser Leu Ala Arg Met Phe Leu Asn Asp Phe Leu Asn Asp GlnAsn 275 280 285 Arg Asp Lys Asn Ser Ile Tyr Ser Gly Leu Glu Ala Phe GlyAsp Val 290 295 300 Lys Leu Glu Asp Thr Tyr Phe Asp Arg Asp Val Glu LysAla Phe Met 305 310 315 320 Lys Ala Ser Ala Glu Leu Phe Asn Gln Lys ThrLys Ala Ser Leu Leu 325 330 335 Val Ser Asn Gln Asn Gly Asn Met Tyr ThrSer Ser Val Tyr Gly Ser 340 345 350 Leu Ala Ser Val Leu Ala Gln Tyr SerPro Gln Gln Leu Ala Gly Lys 355 360 365 Arg Ile Gly Val Phe Ser Tyr GlySer Gly Leu Ala Ala Thr Leu Tyr 370 375 380 Ser Leu Lys Val Thr Gln AspAla Thr Pro Gly Ser Ala Leu Asp Lys 385 390 395 400 Ile Thr Ala Ser LeuCys Asp Leu Lys Ser Arg Leu Asp Ser Arg Thr 405 410 415 Cys Val Ala ProAsp Val Phe Ala Glu Asn Met Lys Leu Arg Glu Asp 420 425 430 Thr His HisLeu Ala Asn Tyr Ile Pro Gln Cys Ser Ile Asp Ser Leu 435 440 445 Phe GluGly Thr Trp Tyr Leu Val Arg Val Asp Glu Lys His Arg Arg 450 455 460 ThrTyr Ala Arg Arg Pro Ser Thr Asn Asp His Ser Leu Asp Glu Gly 465 470 475480 Val Gly Leu Val His Ser Asn Thr Ala Thr Glu His Ile Pro Ser Pro 485490 495 Ala Lys Lys Val Pro Arg Leu Pro Ala Thr Ser Gly Glu Pro Glu Ser500 505 510 Ala Val Ile Ser Asn Gly Glu His 515 520 15 3275 DNA Rattusnorvegicus Cytosolic 3-hydroxy 3-methylglutaryl coenzyme A synthase 15cccagggtcc gatcgcgttt ggtgcctgaa ggaggaaccg gtgacagacc tggagactac 60agttctctgt ccttcacaca gctctttcac catgcctggg tcacttcctt tgaatgcaga 120ggcttgctgg ccaaaagatg tgggaatcgt tgcccttgaa atctactttc cttctcagta 180tgttgatcaa gctgagttgg aaaaatacga tggtgtagat gctggaaagt ataccattgg 240cctgggccag gccaggatgg gcttctgcac ggatcgcgaa gacatcaact ctctctgcct 300gactgtggtt cagaaactga tggagagaaa tagcctttcc tatgactgca ttgggcggct 360ggaagtcggg acagagacaa tcatcgacaa atcaaaatcc gtgaagtcta atttgatgca 420gctgtttgag gagtctggga atacagatat agaaggaata gatacaacta atgcatgcta 480tgggggcaca gccgcagtct tcaatgctgt gaactggatc gaatccagct cttgggatgg 540acgatacgct ttggtagttg caggagacat cgctatatat gcctcaggaa acgccaggcc 600tacaggtgga gttggagctg tggctctgct aattgggcca aatgctcctg taatttttga 660ccgagggctt cgtgggacac acatgcagca tgcctacgac ttttacaagc ctgacatgct 720ctctgaatac cctgtggtag atggaaaact ctccatacag tgctacctca gcgcattgga 780ccgctgctat tctgtctacc gcaaaaagat ccgggcccag tggcagaaag agggaaagga 840taaagatttt accctgaatg attttggctt catgatcttt cactcgccat actgtaaact 900ggtgcagaaa tctctagcta ggatgttcct gaatgacttt cttaacgatc aaaacagaga 960caaaaacagt atttacagtg ggctggaagc ctttggggat gtgaaattag aagatactta 1020cttcgacaga gatgtggaaa aggcatttat gaaggctagt gctgagctat tcaaccagaa 1080aacaaaggca tctttgcttg tatcgaatca aaatggaaac atgtacacat cctctgtata 1140cggttccctt gcttctgttc tggcacagta ctcacctcaa cagttggccg ggaagaggat 1200tggagtgttc tcttacggtt ctggcttggc tgccacactc tactccctta aagtcacaca 1260agatgccaca ccaggatctg ctcttgacaa aataacagca agtttatgtg accttaagtc 1320aaggcttgac tcaagaacgt gtgtggcacc agacgtcttt gctgaaaaca tgaagctcag 1380agaggacaca catcacttag ccaactatat tccccagtgt tcaatagatt cactcttcga 1440aggaacatgg tatctagtca gagtggatga aaagcacaga agaacttacg cccggcgtcc 1500ctccacaaat gaccacagtt tggatgaagg agtgggactt gtgcattcaa acacagctac 1560agagcatatt ccaagcccgg ctaagaaagt gccaagactt cctgcgacct cgggcgaacc 1620tgagtcggct gtcatcagta acggggagca ctgagagccg tggccttcac agaggctcgg 1680ggctggatgg ggtatgggaa acgttagagg aatggatgtc ttgggacaat tttacagatt 1740acgtgttgct taaaaatgta atgtaactga cacagagccc agaaaactgt tgtgtttttg 1800gagaagtctc gctgagctcc taacacactt cctgctgtgg gctggccaat ggtgaatgta 1860ctgcgatggt gttaagggct ctgcagaacg tcatacctcg ctgcatgttt acacgcatgc 1920gggttaggct tcaaactcgg tctgaactga gtgcttctga ctgcaaaggc agaggtactg 1980ctgtccagtt taaaaaattg tttttttttt ttttaatgtg taagaatttt tatacttaaa 2040taaaaaaaag tacctgtagc ttttggggga aaaaaaaaaa cctttttcta ggttggggat 2100tgtggaattt aaatgttaca cataaactct gcttaatggc aaggcaaaca tttatctttt 2160tcgaagattt ataaatcctg aagagaaaaa aagagggtat ggttctagga tctggatgaa 2220ccatcagtga gaaaggttag tcataatcaa gtgagcagaa ggatgctggc gttgagcagg 2280cctctgtcac agcaaccagg gctctgtggg cacagctgag ggaactttct ggccaggtgc 2340ccgtgactgc tgctcagctg cactgagatg cagtggagct gctgcacgga agcttgctgt 2400ggtgctgaac gccttacctg cggataaagt gtaaagtagg agggatgggc agggcactat 2460taggttacag tgttacagac ccaggttata gacttgacag ctcaaactca ccagacacct 2520ttttcccttt gtggtttgtg tattttttgt gttttgtttg ttttttttta tattgttcaa 2580tttaaaaaat ttagaaaatt ttaaccttac gttttcacat agtgtgatta gccaaaagga 2640atttcacttc aagatctaga aatagaattc ataacatttt ttcctaaact ttgactttta 2700aaacaacgaa aattaccaca tgagatgaac aagaaaattc attagaaagt tctctgggtg 2760atttttggtg ctgaactgac atgagcctca tagactgtaa aacagaggta gttgaaacta 2820atgtacagaa ctacattttt taattttatt tgcatttaat tctgtgaagt ttcagttatc 2880taaaataaac acataaacgt gtaatgtttc agattgcaag gtgagatgta atgtagcatt 2940tgtaagatat tcttgtcaat attaactggt aggattttga tttgtacagt tttaattggt 3000taaaatgatc tcattttaac atccactgct atagatgaat gatgtaagct cagatttaat 3060gaatggtggg gaaatggtgc atgtaatttt ttcgcaagta tcgagagttc tgtatgtttt 3120gaaaagaata atttaacgtt tgggttgcca ggaagggggc tttcccagag tccattgcca 3180ggcgttgggc aagcctcgca atgttggcac ggagcgttaa ccacacctta ctaatagcaa 3240ggggaataac tttgaaataa agttttagac aaata 3275 16 353 PRT Rattus norvegicusfarnesyl pyrophosphate synthetase 16 Met Asn Gly Asp Gln Lys Leu Asp ValHis Asn Gln Glu Lys Gln Asn 1 5 10 15 Phe Ile Gln His Phe Ser Gln IleVal Lys Val Leu Thr Glu Asp Glu 20 25 30 Leu Gly His Pro Glu Lys Gly AspAla Ile Thr Arg Ile Lys Glu Val 35 40 45 Leu Glu Tyr Asn Thr Val Gly GlyLys Tyr Asn Arg Gly Leu Thr Val 50 55 60 Val Gln Thr Phe Gln Glu Leu ValGlu Pro Arg Lys Gln Asp Ala Glu 65 70 75 80 Ser Leu Gln Arg Ala Leu ThrVal Gly Trp Cys Val Glu Leu Leu Gln 85 90 95 Ala Phe Phe Leu Val Leu AspAsp Ile Met Asp Ser Ser His Thr Arg 100 105 110 Arg Gly Gln Ile Cys TrpTyr Gln Lys Pro Gly Ile Gly Leu Asp Ala 115 120 125 Ile Asn Asp Ala LeuLeu Leu Glu Ala Ala Ile Tyr Arg Leu Leu Lys 130 135 140 Phe Tyr Cys ArgGlu Gln Pro Tyr Tyr Leu Asn Leu Leu Glu Leu Phe 145 150 155 160 Leu GlnSer Ser Tyr Gln Thr Glu Ile Gly Gln Thr Leu Asp Leu Ile 165 170 175 ThrAla Pro Gln Gly Gln Val Asp Leu Gly Arg Tyr Thr Glu Lys Arg 180 185 190Tyr Lys Ser Ile Val Lys Tyr Lys Thr Ala Phe Tyr Ser Phe Tyr Leu 195 200205 Pro Ile Ala Ala Ala Met Tyr Met Ala Gly Ile Asp Gly Glu Lys Glu 210215 220 His Ala Asn Ala Leu Lys Ile Leu Leu Glu Met Gly Glu Phe Phe Gln225 230 235 240 Ile Gln Asp Asp Tyr Leu Asp Leu Phe Gly Asp Pro Ser ValThr Gly 245 250 255 Lys Val Gly Thr Asp Ile Gln Asp Asn Lys Cys Ser TrpLeu Val Val 260 265 270 Gln Cys Leu Leu Arg Ala Thr Pro Gln Gln Arg GlnIle Leu Glu Glu 275 280 285 Asn Tyr Gly Gln Lys Asp Pro Glu Lys Val AlaArg Val Lys Ala Leu 290 295 300 Tyr Glu Glu Leu Asp Leu Arg Ser Val PhePhe Lys Tyr Glu Glu Asp 305 310 315 320 Ser Tyr Asn Arg Leu Lys Ser LeuIle Glu Gln Cys Ser Ala Pro Leu 325 330 335 Pro Pro Ser Ile Phe Leu GluLeu Ala Asn Lys Ile Tyr Lys Arg Arg 340 345 350 Lys 17 1271 DNA Rattusnorvegicus farnesyl pyrophosphate synthetase 17 ttatatttgg gttctgcctactgagccggg agtctgggaa ctacaactcc cagagtgctg 60 agcggatgca cgctctgcttttaggtgtaa gccgcaaaca tcttggaccc cgggagaatc 120 cgcgttgaag cacagagcatttagctcctc tgtcagaatg aatggggacc agaaactgga 180 tgttcataac caagaaaagcagaatttcat ccagcacttc tcccagattg tcaaggtgct 240 gactgaggat gaactgggacacccagagaa gggagatgct attacccgga tcaaagaggt 300 cctggagtac aacactgtaggaggcaagta caatcggggt ctgacggtgg tacagacctt 360 ccaggaactg gtggaaccaaggaaacagga tgctgagagc ctacagcggg ccctgacggt 420 gggctggtgt gtagaactgctccaggcttt cttcctcgtg ttagatgaca tcatggactc 480 ttcccacact cgccgggggcagatctgctg gtatcagaag ccgggcatag gcttggatgc 540 catcaacgat gctctgcttctggaagccgc tatctaccgc ctgcttaagt tctactgcag 600 ggagcagccc tactacctcaacctgctgga gctctttcta cagagttcct atcagactga 660 gatcgggcag actctcgacctcatcacagc accccagggc caagtggatc ttggtagata 720 cactgaaaag aggtacaaatctatcgtcaa gtacaagaca gctttctact ctttctacct 780 gcctatcgcg gctgccatgtacatggctgg aattgatggg gagaaggaac acgctaatgc 840 cctgaagatc ctgctggagatgggcgagtt cttccagatc caggacgact accttgatct 900 ctttggagac cccagtgtgaccggaaaggt cggcactgac atccaggaca acaaatgcag 960 ctggctggtg gttcagtgtctgctacgagc cactcctcag cagcgccaga tcttagagga 1020 gaattatggg cagaaggacccagaaaaagt ggcgcgggtg aaagcactgt acgaggagct 1080 ggatctgcgg agtgtgttcttcaagtacga ggaagacagt tacaaccgcc tcaagagtct 1140 catagagcag tgctccgcgcccctgccccc atccatcttc ctggaactag caaacaagat 1200 ctacaagcgg agaaagtaacctcgaattgt agaggctgcg agggaggggt ctcaataaat 1260 tattgttcaa c 1271 18152 PRT Rattus norvegicus Bendless protein 18 Met Ala Gly Leu Pro ArgArg Ile Ile Lys Glu Thr Gln Arg Leu Leu 1 5 10 15 Ala Glu Pro Val ProGly Ile Lys Ala Glu Pro Asp Glu Ser Asn Ala 20 25 30 Arg Tyr Phe His ValVal Ile Ala Gly Pro Gln Asp Ser Pro Phe Glu 35 40 45 Gly Gly Thr Phe LysLeu Glu Leu Phe Leu Pro Glu Glu Tyr Pro Met 50 55 60 Ala Ala Pro Lys ValArg Phe Met Thr Lys Ile Tyr His Pro Asn Val 65 70 75 80 Asp Lys Leu GlyArg Ile Cys Leu Asp Ile Leu Lys Asp Lys Trp Ser 85 90 95 Pro Ala Leu GlnIle Arg Thr Val Leu Leu Ser Ile Gln Ala Leu Leu 100 105 110 Ser Ala ProAsn Pro Asp Asp Pro Leu Ala Asn Asp Val Ala Glu Gln 115 120 125 Trp LysSer Asn Glu Ala Gln Ala Ile Glu Thr Ala Arg Ala Trp Thr 130 135 140 ArgLeu Tyr Ala Met Asn Asn Ile 145 150 19 459 DNA Rattus norvegicusBendless protein 19 atggccgggc tgccccgcag gatcatcaag gaaacccagcgtttgctggc agaaccagtt 60 cctggcatta aagcagaacc agatgagagc aacgcccgttattttcatgt ggtcattgct 120 ggtccccagg attccccctt tgagggaggg acttttaaacttgaactatt ccttccagaa 180 gaatacccaa tggcagcacc taaagtacgt ttcatgaccaaaatttatca tcctaatgta 240 gacaagttgg gaagaatatg tttagatatt ttgaaagataagtggtcccc agcactgcag 300 atccgaacag ttctgctatc aatccaggct ttgctaagtgctcctaatcc agatgatcca 360 ttagcaaatg atgtagctga gcagtggaag agcaacgaagcccaagccat agaaacagca 420 agagcatgga ctaggctata tgccatgaat aatatttaa 45920 2505 PRT Rattus norvegicus Fatty acid synthase 20 Met Glu Glu Val ValIle Ala Gly Met Ser Gly Lys Leu Pro Glu Ser 1 5 10 15 Glu Asn Leu GlnGlu Phe Trp Ala Asn Leu Ile Gly Gly Val Asp Met 20 25 30 Val Thr Asp AspAsp Arg Arg Trp Lys Ala Gly Leu Tyr Gly Leu Pro 35 40 45 Lys Arg Ser GlyLys Leu Lys Asp Leu Ser Lys Phe Asp Ala Ser Phe 50 55 60 Phe Gly Val HisPro Lys Gln Ala His Thr Met Asp Pro Gln Leu Arg 65 70 75 80 Leu Leu LeuGlu Val Ser Tyr Glu Ala Ile Val Asp Gly Gly Ile Asn 85 90 95 Pro Ala SerLeu Arg Gly Thr Asn Thr Gly Val Trp Val Gly Val Ser 100 105 110 Gly SerGlu Ala Ser Glu Ala Leu Ser Arg Asp Pro Glu Thr Leu Leu 115 120 125 GlyTyr Ser Met Val Gly Cys Gln Arg Ala Met Met Ala Asn Arg Leu 130 135 140Ser Phe Phe Phe Asp Phe Lys Gly Pro Ser Ile Ala Leu Asp Thr Ala 145 150155 160 Cys Ser Ser Ser Leu Leu Ala Leu Gln Asn Ala Tyr Gln Ala Ile Arg165 170 175 Ser Gly Glu Cys Pro Ala Ala Ile Val Gly Gly Ile Asn Leu LeuLeu 180 185 190 Lys Pro Asn Thr Ser Val Gln Phe Met Lys Leu Gly Met LeuSer Pro 195 200 205 Asp Gly Thr Cys Arg Ser Phe Asp Asp Ser Gly Asn GlyTyr Cys Arg 210 215 220 Ala Glu Ala Val Val Ala Val Leu Leu Thr Lys LysSer Leu Ala Arg 225 230 235 240 Arg Val Tyr Ala Thr Ile Leu Asn Ala GlyThr Asn Thr Asp Gly Cys 245 250 255 Lys Glu Gln Gly Val Thr Phe Pro SerGly Glu Ala Gln Glu Gln Leu 260 265 270 Ile Arg Ser Leu Tyr Gln Pro GlyGly Val Ala Pro Glu Ser Leu Glu 275 280 285 Tyr Ile Glu Ala His Gly ThrGly Thr Lys Val Gly Asp Pro Gln Glu 290 295 300 Leu Asn Gly Ile Thr ArgSer Leu Cys Ala Phe Arg Gln Ser Pro Leu 305 310 315 320 Leu Ile Gly SerThr Lys Ser Asn Met Gly His Pro Glu Pro Ala Ser 325 330 335 Gly Leu AlaAla Leu Thr Lys Val Leu Leu Ser Leu Glu Asn Gly Val 340 345 350 Trp AlaPro Asn Leu His Phe His Asn Pro Asn Pro Glu Ile Pro Ala 355 360 365 LeuLeu Asp Gly Arg Leu Gln Val Val Asp Arg Pro Leu Pro Val Arg 370 375 380Gly Gly Ile Val Gly Ile Asn Ser Phe Gly Phe Gly Gly Ala Asn Val 385 390395 400 His Val Ile Leu Gln Pro Asn Thr Gln Gln Ala Pro Ala Pro Ala Pro405 410 415 His Ala Ala Leu Pro His Leu Leu His Ala Ser Gly Arg Thr MetGlu 420 425 430 Ala Val Gln Gly Leu Leu Glu Gln Gly Arg Gln His Ser GlnAsp Leu 435 440 445 Ala Phe Val Ser Met Leu Asn Asp Ile Ala Ala Thr ProThr Ala Ala 450 455 460 Met Pro Phe Arg Gly Tyr Thr Val Leu Gly Val GluGly His Val Gln 465 470 475 480 Glu Val Gln Gln Val Pro Ala Ser Gln ArgPro Leu Trp Phe Ile Cys 485 490 495 Ser Gly Met Gly Thr Gln Trp Arg GlyMet Gly Leu Ser Leu Met Arg 500 505 510 Leu Asp Ser Phe Arg Glu Ser IleLeu Arg Ser Asp Glu Ala Leu Lys 515 520 525 Pro Leu Gly Val Lys Val SerAsp Leu Leu Leu Ser Thr Asp Glu His 530 535 540 Thr Phe Asp Asp Ile ValHis Ser Phe Val Ser Leu Thr Ala Ile Gln 545 550 555 560 Ile Ala Leu IleAsp Leu Leu Thr Ser Met Gly Leu Lys Pro Asp Gly 565 570 575 Ile Ile GlyHis Ser Leu Gly Glu Val Ala Cys Gly Tyr Ala Asp Gly 580 585 590 Cys LeuSer Gln Arg Glu Ala Val Leu Ala Ala Tyr Trp Arg Gly Gln 595 600 605 CysIle Lys Asp Ala Asn Leu Pro Ala Gly Ser Met Ala Ala Val Gly 610 615 620Leu Ser Trp Glu Glu Cys Lys Gln Arg Cys Pro Pro Gly Val Val Pro 625 630635 640 Ala Cys His Asn Ser Glu Asp Thr Val Thr Ile Ser Gly Pro Gln Ala645 650 655 Ala Val Asn Glu Phe Val Glu Gln Leu Lys Gln Glu Gly Val PheAla 660 665 670 Lys Glu Val Arg Thr Gly Gly Leu Ala Phe His Ser Tyr PheMet Glu 675 680 685 Gly Ile Ala Pro Thr Leu Leu Gln Ala Leu Lys Lys ValIle Arg Glu 690 695 700 Pro Arg Pro Arg Ser Ala Arg Trp Leu Ser Thr SerIle Pro Glu Ala 705 710 715 720 Gln Trp Gln Ser Ser Leu Ala Arg Thr SerSer Ala Glu Tyr Asn Val 725 730 735 Asn Asn Leu Val Ser Pro Val Leu PheGln Glu Ala Leu Trp His Val 740 745 750 Pro Glu His Ala Val Val Leu GluIle Ala Pro His Ala Leu Leu Gln 755 760 765 Ala Val Leu Lys Arg Gly ValLys Pro Ser Cys Thr Ile Ile Pro Leu 770 775 780 Met Lys Arg Asp His LysAsp Asn Leu Glu Phe Phe Leu Thr Asn Leu 785 790 795 800 Gly Lys Val HisLeu Thr Gly Ile Asp Ile Asn Pro Asn Ala Leu Phe 805 810 815 Pro Pro ValGlu Phe Pro Val Pro Arg Gly Thr Pro Leu Ile Ser Pro 820 825 830 His IleLys Trp Asp His Ser Gln Thr Trp Asp Ile Pro Val Ala Glu 835 840 845 AspPhe Pro Asn Gly Ser Ser Ser Ser Ser Ala Thr Val Tyr Asn Ile 850 855 860Asp Ala Ser Ser Glu Ser Ser Asp His Tyr Leu Val Asp His Cys Ile 865 870875 880 Asp Gly Arg Val Leu Phe Pro Gly Thr Gly Tyr Leu Tyr Leu Val Trp885 890 895 Lys Thr Leu Ala Arg Ser Leu Ser Leu Ser Leu Glu Glu Thr ProVal 900 905 910 Val Phe Glu Asn Val Thr Phe His Gln Ala Thr Ile Leu ProArg Thr 915 920 925 Gly Thr Val Pro Leu Glu Val Arg Leu Leu Glu Ala SerHis Ala Phe 930 935 940 Glu Val Ser Asp Ser Gly Asn Leu Ile Val Ser GlyLys Val Tyr Gln 945 950 955 960 Trp Glu Asp Pro Asp Ser Lys Leu Phe AspHis Pro Glu Val Pro Ile 965 970 975 Pro Ala Glu Ser Glu Ser Val Ser ArgLeu Thr Gln Gly Glu Val Tyr 980 985 990 Lys Glu Leu Arg Leu Arg Gly TyrAsp Tyr Gly Pro His Phe Gln Gly 995 1000 1005 Val Tyr Glu Ala Thr LeuGlu Gly Glu Gln Gly Lys Leu Leu Trp Lys 1010 1015 1020 Asp Asn Trp ValThr Phe Met Asp Thr Met Leu Gln Ile Ser Ile Leu 1025 1030 1035 1040 GlyPhe Ser Lys Gln Ser Leu Gln Leu Pro Thr Arg Val Thr Ala Ile 1045 10501055 Tyr Ile Asp Pro Ala Thr His Leu Gln Lys Val Tyr Met Leu Glu Gly1060 1065 1070 Asp Thr Gln Val Ala Asp Val Thr Thr Ser Arg Cys Leu GlyVal Thr 1075 1080 1085 Val Ser Gly Gly Val Tyr Ile Ser Arg Leu Gln ThrThr Ala Thr Ser 1090 1095 1100 Arg Arg Gln Gln Glu Gln Leu Val Pro ThrLeu Glu Lys Phe Val Phe 1105 1110 1115 1120 Thr Pro His Val Glu Pro GluCys Leu Ser Glu Ser Ala Ile Leu Gln 1125 1130 1135 Lys Glu Leu Gln LeuCys Lys Gly Leu Ala Lys Ala Leu Gln Thr Lys 1140 1145 1150 Ala Thr GlnGln Gly Leu Lys Met Thr Val Pro Gly Leu Glu Asp Leu 1155 1160 1165 ProGln His Gly Leu Pro Arg Leu Leu Ala Ala Ala Cys Gln Leu Gln 1170 11751180 Leu Asn Gly Asn Leu Gln Leu Glu Leu Gly Glu Val Leu Ala Arg Glu1185 1190 1195 1200 Arg Leu Leu Leu Pro Glu Asp Pro Leu Ile Ser Gly LeuLeu Asn Ser 1205 1210 1215 Gln Ala Leu Lys Ala Cys Ile Asp Thr Ala LeuGlu Asn Leu Ser Thr 1220 1225 1230 Leu Lys Met Lys Val Val Glu Val LeuAla Gly Glu Gly His Leu Tyr 1235 1240 1245 Ser His Ile Ser Ala Leu LeuAsn Thr Gln Pro Met Leu Gln Leu Glu 1250 1255 1260 Tyr Thr Ala Thr AspArg His Pro Gln Ala Leu Lys Asp Val Gln Thr 1265 1270 1275 1280 Lys LeuGln Gln His Asp Val Ala Gln Gly Gln Trp Asp Pro Ser Gly 1285 1290 1295Pro Ala Pro Thr Asn Leu Gly Ala Leu Asp Leu Val Val Cys Asn Cys 13001305 1310 Ala Leu Ala Thr Leu Gly Asp Pro Ala Leu Ala Leu Asp Asn MetVal 1315 1320 1325 Ala Ala Leu Lys Asp Gly Gly Phe Leu Leu Met His ThrVal Leu Lys 1330 1335 1340 Gly His Ala Leu Gly Glu Thr Leu Ala Cys LeuPro Ser Glu Val Gln 1345 1350 1355 1360 Pro Gly Pro Ser Phe Leu Ser GlnGlu Glu Trp Glu Ser Leu Phe Ser 1365 1370 1375 Arg Lys Ala Leu His LeuVal Gly Leu Lys Lys Ser Phe Tyr Gly Thr 1380 1385 1390 Ala Leu Phe LeuCys Arg Arg Leu Ser Pro Gln Asp Lys Pro Ile Phe 1395 1400 1405 Leu ProVal Glu Asp Thr Ser Phe Gln Trp Val Asp Ser Leu Lys Ser 1410 1415 1420Ile Leu Ala Thr Ser Ser Ser Gln Pro Val Trp Leu Thr Ala Met Asn 14251430 1435 1440 Cys Pro Thr Ser Gly Val Val Gly Leu Val Asn Cys Leu ArgLys Glu 1445 1450 1455 Pro Gly Gly His Arg Ile Arg Cys Ile Leu Leu SerAsn Leu Ser Ser 1460 1465 1470 Thr Ser His Val Pro Lys Leu Asp Pro GlySer Ser Glu Leu Gln Lys 1475 1480 1485 Val Leu Glu Ser Asp Leu Val MetAsn Val Tyr Arg Asp Gly Ala Trp 1490 1495 1500 Gly Ala Phe Arg His PheGln Leu Glu Gln Asp Lys Pro Glu Glu Gln 1505 1510 1515 1520 Thr Ala HisAla Phe Val Asn Val Leu Thr Arg Gly Asp Leu Ala Ser 1525 1530 1535 IleArg Trp Val Ser Ser Pro Leu Lys His Met Gln Pro Pro Ser Ser 1540 15451550 Ser Gly Ala Gln Leu Cys Thr Val Tyr Tyr Ala Ser Leu Asn Phe Arg1555 1560 1565 Asp Ile Met Leu Ala Thr Gly Lys Leu Ser Pro Asp Ala IlePro Gly 1570 1575 1580 Lys Trp Ala Ser Arg Asp Cys Met Leu Gly Met GluPhe Ser Gly Arg 1585 1590 1595 1600 Asp Lys Cys Gly Arg Arg Val Met GlyLeu Val Pro Ala Glu Gly Leu 1605 1610 1615 Ala Thr Ser Val Leu Leu SerPro Asp Phe Leu Trp Asp Val Pro Ser 1620 1625 1630 Ser Trp Thr Leu GluGlu Ala Ala Ser Val Pro Val Val Tyr Thr Thr 1635 1640 1645 Ala Tyr TyrSer Leu Val Val Arg Gly Arg Ile Gln His Gly Glu Thr 1650 1655 1660 ValLeu Ile His Ser Gly Ser Gly Gly Val Gly Gln Ala Ala Ile Ser 1665 16701675 1680 Ile Ala Leu Ser Leu Gly Cys Arg Val Phe Thr Thr Val Gly SerAla 1685 1690 1695 Glu Lys Arg Ala Tyr Leu Gln Ala Arg Phe Pro Gln LeuAsp Asp Thr 1700 1705 1710 Ser Phe Ala Asn Ser Arg Asp Thr Ser Phe GluGln His Val Leu Leu 1715 1720 1725 His Thr Gly Gly Lys Gly Val Asp LeuVal Leu Asn Ser Leu Ala Glu 1730 1735 1740 Glu Lys Leu Gln Ala Ser ValArg Cys Leu Ala Gln His Gly Arg Phe 1745 1750 1755 1760 Leu Glu Ile GlyLys Phe Asp Leu Ser Asn Asn His Pro Leu Gly Met 1765 1770 1775 Ala IlePhe Leu Lys Asn Val Thr Phe His Gly Ile Leu Leu Asp Ala 1780 1785 1790Leu Phe Glu Gly Ala Asn Asp Ser Trp Arg Glu Val Ala Glu Leu Leu 17951800 1805 Lys Ala Gly Ile Arg Asp Gly Val Val Lys Pro Leu Lys Cys ThrVal 1810 1815 1820 Phe Pro Lys Ala Gln Val Glu Asp Ala Phe Arg Tyr MetAla Gln Gly 1825 1830 1835 1840 Lys His Ile Gly Lys Val Leu Val Gln ValArg Glu Glu Glu Pro Glu 1845 1850 1855 Ala Met Leu Pro Gly Ala Gln ProThr Leu Ile Ser Ala Ile Ser Lys 1860 1865 1870 Thr Phe Cys Pro Glu HisLys Ser Tyr Ile Ile Thr Gly Gly Leu Gly 1875 1880 1885 Gly Phe Gly LeuGlu Leu Ala Arg Trp Leu Val Leu Arg Gly Ala Gln 1890 1895 1900 Arg LeuVal Leu Thr Ser Arg Ser Gly Ile Arg Thr Gly Tyr Gln Ala 1905 1910 19151920 Lys His Val Arg Glu Trp Arg Arg Gln Gly Ile His Val Leu Val Ser1925 1930 1935 Thr Ser Asn Val Ser Ser Leu Glu Gly Ala Arg Ala Leu IleAla Glu 1940 1945 1950 Ala Thr Lys Leu Gly Pro Val Gly Gly Val Phe AsnLeu Ala Met Val 1955 1960 1965 Leu Arg Asp Ala Met Leu Glu Asn Gln ThrPro Glu Leu Phe Gln Asp 1970 1975 1980 Val Asn Lys Pro Lys Tyr Asn GlyThr Leu Asn Leu Asp Arg Ala Thr 1985 1990 1995 2000 Arg Glu Ala Cys ProGlu Leu Asp Tyr Phe Val Ala Phe Ser Ser Val 2005 2010 2015 Ser Cys GlyArg Gly Asn Ala Gly Gln Ser Asn Tyr Gly Phe Ala Asn 2020 2025 2030 SerThr Met Glu Arg Ile Cys Glu Gln Arg Arg His Asp Gly Leu Pro 2035 20402045 Gly Leu Ala Val Gln Trp Gly Ala Ile Gly Asp Val Gly Ile Ile Leu2050 2055 2060 Glu Ala Met Gly Thr Asn Asp Thr Val Val Gly Gly Thr LeuPro Gln 2065 2070 2075 2080 Arg Ile Ser Ser Cys Met Glu Val Leu Asp LeuPhe Leu Asn Gln Pro 2085 2090 2095 His Ala Val Leu Ser Ser Phe Val LeuAla Glu Lys Lys Ala Val Ala 2100 2105 2110 His Gly Asp Gly Glu Ala GlnArg Asp Leu Val Lys Ala Val Ala His 2115 2120 2125 Ile Leu Gly Ile ArgAsp Leu Ala Gly Ile Asn Leu Asp Ser Ser Leu 2130 2135 2140 Ala Asp LeuGly Leu Asp Ser Leu Met Gly Val Glu Val Arg Gln Ile 2145 2150 2155 2160Leu Glu Arg Glu His Asp Leu Val Leu Pro Ile Arg Glu Val Arg Gln 21652170 2175 Leu Thr Leu Arg Lys Leu Gln Glu Met Ser Ser Lys Ala Gly SerAsp 2180 2185 2190 Thr Glu Leu Ala Ala Pro Lys Ser Lys Asn Asp Thr SerLeu Lys Gln 2195 2200 2205 Ala Gln Leu Asn Leu Ser Ile Leu Leu Val AsnPro Glu Gly Pro Thr 2210 2215 2220 Leu Thr Arg Leu Asn Ser Val Gln SerSer Glu Arg Pro Leu Phe Leu 2225 2230 2235 2240 Val His Pro Ile Glu GlySer Ile Thr Val Phe His Ser Leu Ala Ala 2245 2250 2255 Lys Leu Ser ValPro Thr Tyr Gly Leu Gln Cys Thr Gln Ala Ala Pro 2260 2265 2270 Leu AspSer Ile Pro Asn Leu Ala Ala Tyr Tyr Ile Asp Cys Ile Lys 2275 2280 2285Gln Val Gln Pro Glu Gly Pro Tyr Arg Val Ala Gly Tyr Ser Phe Gly 22902295 2300 Ala Cys Val Ala Phe Glu Met Cys Ser Gln Leu Gln Ala Gln GlnGly 2305 2310 2315 2320 Pro Ala Pro Ala His Asn Asn Leu Phe Leu Phe AspGly Ser His Thr 2325 2330 2335 Tyr Val Leu Ala Tyr Thr Gln Ser Tyr ArgAla Lys Leu Thr Pro Gly 2340 2345 2350 Cys Glu Ala Glu Ala Glu Ala GluAla Ile Cys Phe Phe Ile Lys Gln 2355 2360 2365 Phe Val Asp Ala Glu HisSer Lys Val Leu Glu Ala Leu Leu Pro Leu 2370 2375 2380 Lys Ser Leu GluAsp Arg Val Ala Ala Ala Val Asp Leu Ile Thr Arg 2385 2390 2395 2400 SerHis Gln Ser Leu Asp Arg Arg Asp Leu Ser Phe Ala Ala Val Ser 2405 24102415 Phe Tyr Tyr Lys Leu Arg Ala Ala Asp Gln Tyr Lys Pro Lys Ala Lys2420 2425 2430 Tyr His Gly Asn Val Ile Leu Leu Arg Ala Lys Thr Gly GlyThr Tyr 2435 2440 2445 Gly Glu Asp Leu Gly Ala Asp Tyr Asn Leu Ser GlnVal Cys Asp Gly 2450 2455 2460 Lys Val Ser Val His Ile Ile Glu Gly AspHis Arg Thr Leu Leu Glu 2465 2470 2475 2480 Gly Arg Gly Leu Glu Ser IleIle Asn Ile Ile His Ser Ser Leu Ala 2485 2490 2495 Glu Pro Arg Val SerVal Arg Glu Gly 2500 2505 21 9143 DNA Rattus norvegicus Fatty acidsynthase 21 agagagacgg cagcagcgtc ccgtccagtt cgcctgccgc gctcctcgcttgtcgtctgc 60 ctccagatcc cagacagaga agagccatgg aggaggtggt gatagccggtatgtccggga 120 aattgcccga gtcagagaac ctgcaggagt tctgggccaa cctcattggcggtgtggaca 180 tggtcacaga cgatgacagg aggtggaagg ctgggctcta tgggttgcctaagcggtctg 240 gaaagctgaa ggatctgtcc aagttcgacg cctccttttt tggggtccaccccaagcagg 300 cacacacaat ggacccgcag ctccggctgc tgctggaagt cagctatgaagctattgtgg 360 acggaggtat caacccggcc tcactccgag gaacaaacac tggtgtctgggtgggtgtga 420 gtggttccga ggcgtcggag gccctgagca gagatcctga gactcttctgggctacagca 480 tggtgggctg ccagagagca atgatggcca accggctctc tttcttcttcgacttcaaag 540 gacccagcat tgccctggac acagcctgct cctctagcct actggcactacagaatgcct 600 atcaggctat ccgcagtggg gagtgccctg ctgccattgt gggcgggatcaacctgctgc 660 taaagcctaa cacctctgtg cagttcatga agctaggcat gctcagccccgatggcacct 720 gcagatcctt tgatgattca gggaacgggt attgccgtgc tgaggctgtcgtggcagttc 780 tgctgactaa gaagtccttg gctcggcgag tctatgccac tattctgaatgccgggacga 840 acacagatgg ctgcaaggag caaggcgtga cattcccctc tggagaagcccaggaacaac 900 tcatccgttc tctgtatcag ccgggcggtg tggcccccga gtctcttgaatatattgaag 960 cccatggcac gggcaccaag gtgggggacc cccaggaact gaacggcattactcggtccc 1020 tgtgtgcttt ccgccagagc cctttgttaa ttggctccac caaatccaacatgggacacc 1080 ctgagcctgc ctcggggctt gcagccctga ccaaggtgct gttatccctagaaaatgggg 1140 tttgggcccc caacctgcat ttccacaacc ccaaccctga aatcccagcacttcttgatg 1200 ggcggctgca ggtggtcgat aggcccctgc ctgttcgtgg tggcatcgtgggcatcaact 1260 cgtttggctt cggaggtgcc aatgttcacg tcatcctcca gcccaacacacagcaggccc 1320 cagcacctgc cccacatgct gccctaccgc atttgctgca tgccagtggacggaccatgg 1380 aggcagtgca gggcctgctg gaacagggcc gccagcacag tcaggacttggcctttgtga 1440 gcatgctcaa tgacattgca gcaaccccta cagcagccat gcccttcagaggttacactg 1500 tgttaggtgt tgagggccat gtccaggaag tgcagcaagt gcctgccagccagcgcccac 1560 tctggttcat ctgctcaggg atgggcacac agtggcgtgg aatggggctgagccttatgc 1620 gcctggacag tttccgtgag tccatcctgc gctctgatga ggctctgaagcccttgggag 1680 tcaaagtgtc agacctgctg ctgagcactg atgagcacac ctttgatgacatcgtgcatt 1740 cctttgtgag cctcaccgcc atccagattg ccctcatcga cctgctgacgtctatggggc 1800 tgaaacctga tggcatcatt gggcactcct tgggagaggt tgcctgtggctatgcagatg 1860 gctgtctctc ccagagagag gctgtgcttg cagcctactg gagaggccagtgcattaagg 1920 atgccaacct tccggctgga tccatggcag ctgttggttt gtcctgggaagaatgtaaac 1980 aacgctgccc tcctggtgtg gtgcctgcct gccacaactc tgaggacactgtgaccatct 2040 ctggacctca ggctgcagtg aatgaatttg tggagcagct aaagcaagagggcgtgtttg 2100 ccaaggaggt gcgaacaggt ggcctggcct tccactccta cttcatggaaggaattgccc 2160 ccacgctgct gcaggctctc aagaaggtga tccgggagcc acggccacgctcagcacgct 2220 ggctcagcac ctctatccct gaggcccagt ggcagagcag cctggcccgcacatcttctg 2280 ctgagtacaa cgtcaacaac ctggtgagcc ctgtgctctt ccaggaagcactgtggcacg 2340 tccccgagca cgccgtggtg ctggagattg caccccatgc actgttgcaggctgtcctga 2400 agcgaggcgt gaagcctagc tgcaccatca tccccttgat gaagagggaccataaagata 2460 acttggagtt cttcctcacc aacctcggca aggtgcacct cacaggcatcgacatcaacc 2520 ctaatgcctt gttcccacct gtggaattcc cggttccccg agggactcctctcatctccc 2580 ctcacatcaa gtgggaccac agtcagactt gggatatccc agttgctgaagacttcccca 2640 acggttccag ctcctcctca gctacagtct acaacattga cgccagttccgagtcatctg 2700 accactacct ggtcgaccac tgcattgacg gccgtgtcct cttccctggcactggctacc 2760 tgtacctggt gtggaagaca ctggctcgaa gcctgagctt gtccctagaagagacccctg 2820 tggtgtttga gaacgtgaca tttcatcagg ccaccatcct gcccaggacaggaaccgtgc 2880 ctctggaggt gcggctgcta gaggcctcac atgcatttga ggtgtctgacagtggcaacc 2940 tgatagtgag cgggaaagtg taccagtggg aagaccctga ctccaagttattcgaccacc 3000 cagaagtccc gatccccgcc gagtccgagt ctgtctcccg cttgacgcagggagaagtat 3060 acaaggagct gcggctacgt ggctatgact atggccctca tttccagggcgtctatgagg 3120 ccaccctcga aggtgagcaa ggcaagctgc tctggaaaga caactgggtgaccttcatgg 3180 acacaatgct gcagatatcc atcctgggct tcagcaagca gagtctgcagctacccaccc 3240 gtgtgactgc catctatatt gaccctgcaa cccacctgca gaaggtgtacatgctggagg 3300 gagacactca agtggctgac gtgaccacga gccgctgtct gggcgtgaccgtctctggtg 3360 gtgtctacat ttcgagacta cagacaacag caacctcacg gcggcagcaggaacagctgg 3420 tccccaccct ggagaagttt gtcttcacac cccatgtgga gcctgagtgcctgtctgaga 3480 gtgctatcct gcagaaagag ctgcagctgt gcaagggtct ggcaaaggctctgcagacca 3540 aggccaccca gcaagggctg aagatgacag tgcctgggct agaggaccttccccagcatg 3600 gactgcctcg actcttggct gctgcctgcc agctgcagct caacgggaacctgcaactgg 3660 agttaggtga ggtactggct cgagagaggc tcctgctgcc agaagaccctctgatcagtg 3720 gcctccttaa ctcccaggcc ctcaaggcct gcatagacac agccctggagaacttgtcta 3780 ctctcaagat gaaggtggtg gaggtgctgg ctggagaagg ccacttgtattcccacatct 3840 cagcactgct caacacccag cctatgctgc aactggagta tacagccaccgaccggcacc 3900 cccaggccct gaaggatgtt cagaccaagc tgcagcagca tgatgtagcacagggccagt 3960 gggacccttc tggtcctgct cctaccaacc tgggtgctct tgaccttgtggtgtgcaact 4020 gtgcgttagc caccctgggg gatccagccc tggccctgga caacatggtagctgccctca 4080 aggatggtgg tttcctgcta atgcacacag tgctcaaagg acatgcccttggggagaccc 4140 tggcctgcct cccttctgag gtgcagcctg ggcccagctt cttaagccaggaagagtggg 4200 agagcctgtt ctcaaggaag gcactgcacc tggtgggcct taaaaagtcattctacggta 4260 ctgcgctgtt cctgtgccgc cgtctcagcc cacaggacaa gcccatcttcctgcctgtgg 4320 aggatactag tttccagtgg gtggactctc tgaagagcat tctggccacatcctcctccc 4380 agcctgtgtg gctaacagcc atgaactgcc ccacctcagg tgtggtaggcttggtgaact 4440 gtctccgaaa agagccgggt ggacaccgga ttcggtgtat cctgctgtccaacctcagca 4500 gcacatctca cgtccccaag ctggaccctg gctcttcaga gctacagaaggtgctagaga 4560 gtgatctggt gatgaacgtg tacagggacg gtgcctgggg tgccttccgtcacttccagt 4620 tagagcagga caagcccgag gagcagacag cacatgcctt tgtaaacgtccttacccgag 4680 gggaccttgc ctccatccgc tgggtctctt ctcccctgaa acacatgcagccgccctcga 4740 gctcaggagc acagctctgc actgtctact atgcctcact gaacttccgagatatcatgc 4800 tggccacggg caagctgtcc cctgatgcca ttccaggtaa atgggccagccgggactgca 4860 tgcttggcat ggagttctca ggccgtgata agtgcggccg gcgtgtgatggggctggtac 4920 ccgcagaagg cctggccacc tcagtcctgt tatcacccga cttcctctgggatgtaccct 4980 ctagctggac cctggaggag gcggcttctg tgcctgttgt ctacaccaccgcctactact 5040 ccttagtagt gcgtggtcgt attcagcacg gggaaactgt gctcattcactcgggctccg 5100 gtggtgtggg ccaagcggcc atttccattg cccttagcct gggctgccgagtcttcacca 5160 ctgtgggctc cgctgagaag cgagcttacc tccaggccag attccctcagctggatgaca 5220 ccagctttgc taactctcga gacacatcgt ttgagcagca tgtgttactgcacacaggtg 5280 gcaaaggggt ggacctggtc ctcaactccc tggcagaaga gaagctgcaggccagtgtgc 5340 ggtgcttggc tcagcatggc cgcttcctag agatcggcaa atttgatctttctaacaacc 5400 accctctggg catggccatc ttcttgaaga acgtcacttt ccatgggatcctgctggatg 5460 cactttttga gggggccaac gacagctggc gggaggtggc agagctgctgaaggccggca 5520 tccgtgatgg ggttgtgaag cctctcaagt gtacagtgtt tcccaaggcccaggtggagg 5580 acgccttccg atacatggct caaggaaaac atattggcaa agtccttgtccaggtacggg 5640 aggaggagcc cgaggctatg ctgccagggg ctcagcccac cctgatttccgccatctcca 5700 agaccttctg cccagagcat aagagttaca tcatcactgg tggcctaggtggctttggcc 5760 tggaactggc ccggtggctt gtgcttcgtg gggcccaaag gcttgtactaacttcccgat 5820 ctggaatccg cacaggctac caagccaagc acgttcggga gtggaggcgccagggcatcc 5880 atgtgctagt gtcgacaagc aatgtcagtt cactggaggg ggcccgtgctctcatcgctg 5940 aagccacaaa gcttgggccc gttggaggtg tcttcaacct ggccatggttttaagggatg 6000 ccatgctgga gaaccagact ccagaactct tccaggatgt caacaagcccaagtacaatg 6060 gcaccctgaa ccttgacagg gcgacccggg aagcctgtcc tgagctggactactttgtgg 6120 ccttctcctc tgtaagctgc gggcgtggta atgctggcca atccaactatggcttcgcca 6180 actctaccat ggagcgtatt tgcgaacagc gccggcacga tggcctcccaggtcttgccg 6240 tgcaatgggg tgccattggt gacgtgggca ttatcttgga agcgatgggtaccaatgaca 6300 cagtcgttgg cggcacactg ccacagcgca tctcctcctg catggaggtgctggacctct 6360 tcctgaatca gccccacgca gtcctgagca gttttgtgct ggctgagaagaaagctgtgg 6420 cccatggtga tggtgaagcc cagagggatc tggtgaaagc agtggcacacatcctaggca 6480 tccgcgacct cgcagggatt aacctggaca gctcgctggc agacctcggcctggactcgc 6540 tcatgggtgt ggaagtgcgc cagatcctgg aacgtgaaca tgatctggtgctacccattc 6600 gtgaagtacg gcaactcaca ctgcggaagc ttcaggaaat gtcctccaaggctggctcag 6660 acactgagtt ggcagccccc aagtccaaga atgatacatc cctgaagcaggcccagctga 6720 atctgagtat cctgctggtg aaccctgagg gccctacctt aacacgactcaactcagtgc 6780 agagctctga gcggcctctg ttcctggtgc accccattga aggttccatcactgtgttcc 6840 acagcctggc tgccaagctc agtgtgccca cctacggtct gcagtgcacccaagcggccc 6900 ccctggacag cattccaaac ctggctgcct actacattga ttgcatcaagcaggtgcagc 6960 ctgaggggcc ctaccgagtg gctgggtatt cttttggagc ttgtgtagccttcgagatgt 7020 gctcccagct gcaggcccag cagggcccag cccccgccca caacaacctcttcttgtttg 7080 atggctcaca cacctacgta ttggcgtaca cccagagcta ccgggcaaagctgaccccag 7140 gctgtgaggc tgaggctgaa gctgaagcca tatgcttctt cattaagcagtttgttgatg 7200 cagagcatag caaggtgcta gaggccctgc taccactgaa gagcctggaggaccgggttg 7260 ctgctgctgt ggacctcatc actagaagcc accagagcct ggaccgccgtgacctgagct 7320 ttgctgccgt gtccttctac tacaagcttc gagccgccga ccagtataaacccaaggcca 7380 agtaccacgg caatgtgatc ctgctgcggg ccaagacagg tggcacctacggcgaggact 7440 tgggtgccga ttacaacctg tcccaggtgt gtgatgggaa ggtgtctgtgcacatcattg 7500 agggtgacca ccgtacgctg ctggagggca ggggcctgga gtctatcatcaacatcatcc 7560 acagctccct ggctgagcct cgagtgagtg tacgggaggg ctagacctgcctaccatgaa 7620 gccacgaccc acaccggcca ccagagatgc tccgatcccc accacaccctgagtgcaggg 7680 actggggagg gtcctgctgg tgggaccccc tcaccccagt ggcccagcaccaccccctcc 7740 cctggtggct gctacaaaca ggaccatcac atgtgtccca gccacttagtggggttccca 7800 gagccactga cttggaggca ccctggtctg tgaagagtca gtggaggccagcaagagcca 7860 aactgagcct tttctgccaa gtgacatttg tcacactggt tgtttctccattaaattctc 7920 atatttattg cattgctggg aaagaccgcc caccccaggg ttaactcattccagaacccc 7980 taaagtggga aaagccatgt ggggaaggct gctggctgga gcccctttttgtcttagccc 8040 tgtacccgct cactgcaggg cagggtatgg agagggctgg ttcgcggggaacgaggaccc 8100 cagcagacac tgtagcccat ggcccttggt ccccagcact cccggctgcacccatgatgc 8160 agggcctacc agactctgcg gaccgcaccg ggcactcact gtatttgttttccaagattc 8220 aaattgctgc ttgggttttg aatttactgc agctgtcagt gtaaagaaacatgtctgaac 8280 tgtgtccttt ttacaccaac ctggtaaaaa tgctcttgat gctgtcccgttgccacaatt 8340 aaactgcacg tgagctctgg cttccgttca gtctctttcc agtcccagacctgagtcccc 8400 agagcctcca cagctcttac agtgagaatc aaattggccc actccttggaaggcgtggca 8460 ttctgtcaga gtaaaaggaa agtagagtgt gctgattcac gttcagcgtgtggggctggc 8520 tagagacctt ggcactgtag tgaacagaat gtgtccacct ttaagtcaccctgaaggcat 8580 caccatagct acagcctcac ccaggggtag agaatagtac tgtctacttgttgactacct 8640 ggcagttggt gccagcccct atagaggaaa acagcagtgt gtggccactgtgagaagcat 8700 atccctggaa acaggtgacc agagcagagg gctaacgcct acctgagtcacacaaaactg 8760 accaggcttg agtgtccaga agagtctatc agaaggccac agcattcagtcctatccaca 8820 gagagcagca gactaagttg tctccttgcc agcttagaaa actgcagtgctggggtacag 8880 gtagggtgtt caggaggtcc gggccccagt gattagtcta agactgaagcatctggttgg 8940 ctgtggtccc acctagaaaa ttcttaaagc tcttgtcatg tacttcctgggaaggaccta 9000 ccctgtctca ataatgtctc tagctcgttg gagtctactg actcaaacatttataaagtg 9060 tcctagaaag gcctgactcc cctacaaggc tgtgtgatcc ttcaaactcacatatgtgag 9120 ccaataaaac cttgagactc tag 9143 22 373 PRT Rattusnorvegicus Glutamine synthetase 22 Met Ala Thr Ser Ala Ser Ser His LeuAsn Lys Gly Ile Lys Gln Met 1 5 10 15 Tyr Met Asn Leu Pro Gln Gly GluLys Ile Gln Leu Met Tyr Ile Trp 20 25 30 Val Asp Gly Thr Gly Glu Gly LeuArg Cys Lys Thr Arg Thr Leu Asp 35 40 45 Cys Asp Pro Lys Cys Val Glu GluLeu Pro Glu Trp Asn Phe Asp Gly 50 55 60 Ser Ser Thr Phe Gln Ser Glu GlySer Asn Ser Asp Met Tyr Leu His 65 70 75 80 Pro Val Ala Met Phe Arg AspPro Phe Arg Arg Asp Pro Asn Lys Leu 85 90 95 Val Phe Cys Glu Val Phe LysTyr Asn Arg Lys Pro Ala Glu Thr Asn 100 105 110 Leu Arg His Ser Cys LysArg Ile Met Asp Met Val Ser Ser Gln Arg 115 120 125 Pro Trp Phe Gly MetGlu Gln Glu Tyr Thr Leu Met Gly Thr Asp Gly 130 135 140 His Pro Phe GlyTrp Pro Ser Asn Gly Phe Pro Gly Pro Gln Gly Pro 145 150 155 160 Tyr TyrCys Gly Val Gly Ala Asp Lys Ala Tyr Gly Arg Asp Ile Val 165 170 175 GluAla His Tyr Arg Ala Cys Leu Tyr Ala Gly Ile Lys Ile Thr Gly 180 185 190Thr Asn Ala Glu Val Met Pro Ala Gln Trp Glu Phe Gln Ile Gly Pro 195 200205 Cys Glu Gly Ile Arg Met Gly Asp His Leu Trp Val Ala Arg Phe Ile 210215 220 Leu His Arg Val Cys Glu Asp Phe Gly Val Ile Ala Thr Phe Asp Pro225 230 235 240 Lys Pro Ile Pro Gly Asn Trp Asn Gly Ala Gly Cys His ThrAsn Phe 245 250 255 Ser Thr Lys Ala Met Arg Glu Glu Asn Gly Leu Arg CysIle Glu Glu 260 265 270 Ala Ile Asp Lys Leu Ser Lys Arg His Gln Tyr HisIle Arg Ala Tyr 275 280 285 Asp Pro Lys Gly Gly Leu Asp Asn Ala Arg ArgLeu Thr Gly Phe His 290 295 300 Glu Thr Ser Asn Ile Asn Asp Phe Ser AlaGly Val Ala Asn Arg Ser 305 310 315 320 Ala Ser Ile Arg Ile Pro Arg IleVal Gly Gln Glu Lys Lys Gly Tyr 325 330 335 Phe Glu Asp Arg Arg Pro SerAla Asn Cys Asp Pro Tyr Ala Val Thr 340 345 350 Glu Ala Ile Val Arg ThrCys Leu Leu Asn Glu Thr Gly Asp Glu Pro 355 360 365 Phe Gln Tyr Lys Asn370 23 2793 DNA Rattus norvegicus Glutamine synthetase 23 acagccgagaatgggagtag ggcggagtgt ttgagcagca cacccatttc ctctccgctc 60 ttcgtctcgttctcgtggcc tgtccaccca tccatcatct gccggccacc gctctgaaca 120 ccttccaccatggccacctc agcaagttcc cacttgaaca aaggcatcaa gcagatgtac 180 atgaacctgccccagggcga gaagatccaa ctcatgtata tctgggttga tggtaccggg 240 gaagggctacgctgcaagac ccgtactctg gactgtgacc ccaagtgtgt agaagagtta 300 cccgagtggaactttgatgg ttctagtacg tttcagtctg aaggctccaa cagcgacatg 360 tacctccatcctgtggccat gtttcgagac cccttccgca gagaccccaa caagctggtg 420 ttctgcgaagtattcaagta taaccggaag cccgcagaga ccaacctgag gcacagctgt 480 aagcgtataatggacatggt gagcagccag cgcccctggt ttggaatgga acaggagtat 540 actctcatgggaacagacgg ccaccctttc ggctggcctt ctaatggctt ccctggaccc 600 caaggaccctattactgcgg tgtgggagct gacaaggctt atggccgaga tatcgtggag 660 gctcactaccgggcctgctt gtatgctgga atcaagatca cagggacaaa tgccgaggtt 720 atgcctgcccagtgggaatt ccagatagga ccctgcgaag ggatccgcat gggagatcat 780 ctctgggtagcccgttttat cttgcatcgg gtatgcgaag actttggggt gatagcaacc 840 tttgaccccaagcccattcc agggaactgg aatggggcag gctgccacac caactttagc 900 accaaggccatgcgggagga gaatggtctg aggtgcattg aggaggccat tgataaactg 960 agcaagaggcaccagtacca catccgtgcc tacgacccca aggggggcct ggacaacgcc 1020 cgccgtctgactggattcca cgaaacctcc aacatcaacg acttttccgc tggcgttgcc 1080 aaccgcagcgccagtatccg cattccccgg attgtcggcc aggagaagaa gggttacttt 1140 gaagaccgtcggccttctgc caattgcgac ccctatgcgg tgacggaagc catcgtccgc 1200 acgtgtctcctcaacgaaac tggcgacgag cccttccaat acaagaacta agcggactcg 1260 acttccagtgatcttgagcc cttcctagtt caccccactc ccaactgttc cctctcccac 1320 tggtccccactgtaactcaa aaggatggaa taccaaggtc tttttattcc ttgcgcccag 1380 ttaatttttgcctttattgg tcagaataga ggggtcaggt tcttaatctc tacacaccca 1440 accccttctttcctagctag ctttccagtg ggggaacggg agggggtggg gaagggtaac 1500 ccaccgcttcatctcagcgg gaatgcatgt cctgtaggca tagctgtcac aaatcgggtg 1560 tacttgtggtgagggaggac tggttttttt tttccttcag gataattgaa agggcaggcc 1620 caacagcttagattaacatt ttctctgtca gtagagagct gttatttctt ccggtgaaac 1680 cagctttctattgaagtctg gtgaggagtt ggaggttggt ctcttggctt ccttagctta 1740 gggaaggggagttcaccctc ccttcatgaa acacagttca cctgacaaat ggccctactg 1800 taaaggaagaaaaaagtttc ttggtcctcc atttataact caaagcagag tagtattttt 1860 atatttaaatgttaaaaaca aaaaagttat atatatgggt gtgtggatat atatgtcttt 1920 tctaattgagaaaaccatcc tattccctgg gtgccaagtt tgagtgagga gctcggtgta 1980 gaagtgaggcactcttgagg taggggtggg gatgcagtac tgggaaagtt ggttatcttg 2040 ggggttcagcttcattacta cttagggttt ccctgcccac tctgcaggag cagatgttgg 2100 acaggtagccagtgggatgc cactgcttgc cgccactgtc cctgggctta gtttaagggg 2160 acgtgtatacctaatccaca cacgagttag aagtatgagt tggctggtca acttgaacat 2220 tgttacaggcgggtgggtgt tagtgggggg ttattttttg gtgggactag catgtcacta 2280 aagcgggccttttgatatat taaatttttt aaagcaaaac aagtttagat tttaatcaag 2340 ttcgtagggtttctaacttt acagaattgc ctgtttgttt caatgactcc ttccacttgg 2400 ctcttaggggaactgaggac aggcctggag ttaatacact tgtcattctg tgtcctagtg 2460 tcctcttcctccggcagact gtccccttcc ttctgaaaaa gccgatagag tcttgtttta 2520 tttttcttttataataaaca caccccacct ccatcccagc ttgttgcctt gcagttttct 2580 ggatgtttgtgtcggcagca ggcagctgtg gtttttttct cttgccacga tgactctaat 2640 taccatgtatagtatgttca gttagataac tcactgtaaa cagactgtaa ctgagagcag 2700 agcttgtaaatcaacctaac gtttataaga tttcctctga cttgtttctt tgtggttcca 2760 aaaaaaaaaaaaaaaaaaaa aacctcaaaa act 2793 24 514 PRT Homo sapiens Seryl t-RNAsynthetase 24 Met Val Leu Asp Leu Asp Leu Phe Arg Val Asp Lys Gly GlyAsp Pro 1 5 10 15 Ala Leu Ile Arg Glu Thr Gln Glu Lys Arg Phe Lys AspPro Gly Leu 20 25 30 Val Asp Gln Leu Val Lys Ala Asp Ser Glu Trp Arg ArgCys Arg Phe 35 40 45 Arg Ala Asp Asn Leu Ser Lys Leu Lys Asn Leu Cys SerLys Thr Ile 50 55 60 Gly Glu Lys Met Lys Lys Lys Glu Pro Val Gly Asp AspGlu Ser Val 65 70 75 80 Pro Glu Asn Val Leu Ser Phe Asp Asp Leu Thr AlaAsp Ala Leu Ala 85 90 95 Asn Leu Lys Val Ser Gln Ile Lys Lys Val Arg LeuLeu Ile Asp Glu 100 105 110 Ala Ile Leu Lys Cys Asp Ala Glu Arg Ile LysLeu Glu Ala Glu Arg 115 120 125 Phe Glu Asn Leu Arg Glu Ile Gly Asn LeuLeu His Pro Ser Val Pro 130 135 140 Ile Ser Asn Asp Glu Asp Val Asp AsnLys Val Glu Arg Ile Trp Gly 145 150 155 160 Asp Cys Thr Val Arg Lys LysTyr Ser His Val Asp Leu Val Val Met 165 170 175 Val Asp Gly Phe Glu GlyGlu Lys Gly Ala Val Val Ala Gly Ser Arg 180 185 190 Gly Tyr Phe Leu LysGly Val Leu Val Phe Leu Glu Gln Ala Leu Ile 195 200 205 Gln Tyr Ala LeuArg Thr Leu Gly Ser Arg Gly Tyr Ile Pro Ile Tyr 210 215 220 Thr Pro PhePhe Met Arg Lys Glu Val Met Gln Glu Val Ala Gln Leu 225 230 235 240 SerGln Phe Asp Glu Glu Leu Tyr Lys Val Ile Gly Lys Gly Ser Glu 245 250 255Lys Ser Asp Asp Asn Ser Tyr Asp Glu Lys Tyr Leu Ile Ala Thr Ser 260 265270 Glu Gln Pro Ile Ala Ala Leu His Arg Asp Glu Trp Leu Arg Pro Glu 275280 285 Asp Leu Pro Ile Lys Tyr Ala Gly Leu Ser Thr Cys Phe Arg Gln Glu290 295 300 Val Gly Ser His Gly Arg Asp Thr Arg Gly Ile Phe Arg Val HisGln 305 310 315 320 Phe Glu Lys Ile Glu Gln Phe Val Tyr Ser Ser Pro HisAsp Asn Lys 325 330 335 Ser Trp Glu Met Phe Glu Glu Met Ile Thr Thr AlaGlu Glu Phe Tyr 340 345 350 Gln Ser Leu Gly Ile Pro Tyr His Ile Val AsnIle Val Ser Gly Ser 355 360 365 Leu Asn His Ala Ala Ser Lys Lys Leu AspLeu Glu Ala Trp Phe Pro 370 375 380 Gly Ser Gly Ala Phe Arg Glu Leu ValSer Cys Ser Asn Cys Thr Asp 385 390 395 400 Tyr Gln Ala Arg Arg Leu ArgIle Arg Tyr Gly Gln Thr Lys Lys Met 405 410 415 Met Asp Lys Val Glu PheVal His Met Leu Asn Ala Thr Met Cys Ala 420 425 430 Thr Thr Arg Thr IleCys Ala Ile Leu Glu Asn Tyr Gln Thr Glu Lys 435 440 445 Gly Ile Thr ValPro Glu Lys Leu Lys Glu Phe Met Pro Pro Gly Leu 450 455 460 Gln Glu LeuIle Pro Phe Val Lys Pro Ala Pro Ile Glu Gln Glu Pro 465 470 475 480 SerLys Lys Gln Lys Lys Gln His Glu Gly Ser Lys Lys Lys Ala Ala 485 490 495Ala Arg Asp Val Thr Leu Glu Asn Arg Leu Gln Asn Met Glu Val Thr 500 505510 Asp Ala 25 1846 DNA Homo sapiens Seryl t-RNA synthetase 25gcagtgcggc ggtcacaggc tgagtgctgc ggcgcgatcc ttgcttccct gagcgttggc 60ccgggaggaa agaagatggt gctggatctg gatttgtttc gggtggataa aggaggggac 120ccagccctca tccgagagac gcaggagaag cgcttcaagg acccgggact agtggaccag 180ctggtgaagg cagacagcga gtggcgacga tgtagatttc gggcagacaa cttgagcaag 240ctgaagaacc tatgcagcaa gacaatcgga gagaaaatga agaaaaaaga gccagtggga 300gatgatgagt ctgtcccaga gaatgtgctg agtttcgatg accttactgc agacgcttta 360gctaacctga aagtctcaca aatcaaaaaa gtccgactcc tcattgatga agccatcctg 420aagtgtgacg cggagcggat aaagttggaa gcagagcggt ttgagaacct ccgagagatt 480gggaaccttc tgcacccttc tgtacccatc agtaacgatg aggatgtgga caacaaagta 540gagaggattt ggggcgattg tacagtcagg aagaagtact ctcatgtgga cctggtggtg 600atggtagatg gctttgaagg cgaaaagggg gccgtggtgg ctgggagtcg agggtacttc 660ttgaaggggg tcctggtgtt cctggaacag gctctcatcc agtatgccct tcgcaccttg 720ggaagtcggg gctacattcc catttatacc ccctttttca tgaggaagga ggtcatgcag 780gaggtggcac agctcagcca gtttgatgaa gaactttata aggtgattgg caaaggcagt 840gaaaagtctg atgacaactc ctatgatgag aagtacctga ttgccacctc agagcagccc 900attgctgccc tgcaccggga tgagtggctc cggccggagg acctgcccat caagtatgct 960ggcctgtcta cctgcttccg tcaggaggtg ggctcccatg gccgtgacac ccgtggcatc 1020ttccgagtcc atcagtttga gaagattgaa cagtttgtgt actcatcacc ccatgacaac 1080aagtcatggg agatgtttga agagatgatt accaccgcag aggagttcta ccagtccctg 1140gggattcctt accacattgt gaatattgtc tcaggttctt tgaatcatgc tgccagtaag 1200aagcttgacc tggaggcctg gtttccgggc tcaggagcct tccgtgagtt ggtctcctgt 1260tctaattgca cggattacca ggctcgccgg cttcgaatcc gatatgggca aaccaagaag 1320atgatggaca aggtggagtt tgtccatatg ctcaatgcta ccatgtgcgc cactacccgt 1380accatctgcg ccatcctgga gaactaccag acagagaagg gcatcactgt gcctgagaaa 1440ttgaaggagt tcatgccgcc aggactgcaa gaactgatcc cctttgtgaa gcctgcgccc 1500attgagcagg agccatcaaa gaagcagaag aagcaacatg agggcagcaa aaagaaagca 1560gcagcaagag acgtcaccct agaaaacagg ctgcagaaca tggaggtcac cgatgcttga 1620acattcctgc ctccctattt gccaggcttt catttctgtc tgctgagatc tcagagcctg 1680cccaacagca gggaagccaa gcacccattc atccccctgc ccccatctga ctgcgtagct 1740gagaggggaa cagtgccatg taccacacag atgttcctgt ctcctcgcat gggcataggg 1800acccatcatt gatgactgat gaaaccatgt aataaagcat ctctgg 1846 26 434 PRT Homosapiens Alpha-enolase 26 Met Ser Ile Leu Lys Ile His Ala Arg Glu Ile PheAsp Ser Arg Gly 1 5 10 15 Asn Pro Thr Val Glu Val Asp Leu Phe Thr SerLys Gly Leu Phe Arg 20 25 30 Ala Ala Val Pro Ser Gly Ala Ser Thr Gly IleTyr Glu Ala Leu Glu 35 40 45 Leu Arg Asp Asn Asp Lys Thr Arg Tyr Met GlyLys Gly Val Ser Lys 50 55 60 Ala Val Glu His Ile Asn Lys Thr Ile Ala ProAla Leu Val Ser Lys 65 70 75 80 Lys Leu Asn Val Thr Glu Gln Glu Lys IleAsp Lys Leu Met Ile Glu 85 90 95 Met Asp Gly Thr Glu Asn Lys Ser Lys PheGly Ala Asn Ala Ile Leu 100 105 110 Gly Val Ser Leu Ala Val Cys Lys AlaGly Ala Val Glu Lys Gly Val 115 120 125 Pro Leu Tyr Arg His Ile Ala AspLeu Ala Gly Asn Ser Glu Val Ile 130 135 140 Leu Pro Val Pro Ala Phe AsnVal Ile Asn Gly Gly Ser His Ala Gly 145 150 155 160 Asn Lys Leu Ala MetGln Glu Phe Met Ile Leu Pro Val Gly Ala Ala 165 170 175 Asn Phe Arg GluAla Met Arg Ile Gly Ala Glu Val Tyr His Asn Leu 180 185 190 Lys Asn ValIle Lys Glu Lys Tyr Gly Lys Asp Ala Thr Asn Val Gly 195 200 205 Asp GluGly Gly Phe Ala Pro Asn Ile Leu Glu Asn Lys Glu Gly Leu 210 215 220 GluLeu Leu Lys Thr Ala Ile Gly Lys Ala Gly Tyr Thr Asp Lys Val 225 230 235240 Val Ile Gly Met Asp Val Ala Ala Ser Glu Phe Phe Arg Ser Gly Lys 245250 255 Tyr Asp Leu Asp Phe Lys Ser Pro Asp Asp Pro Ser Arg Tyr Ile Ser260 265 270 Pro Asp Gln Leu Ala Asp Leu Tyr Lys Ser Phe Ile Lys Asp TyrPro 275 280 285 Val Val Ser Ile Glu Asp Pro Phe Asp Gln Asp Asp Trp GlyAla Trp 290 295 300 Gln Lys Phe Thr Ala Ser Ala Gly Ile Gln Val Val GlyAsp Asp Leu 305 310 315 320 Thr Val Thr Asn Pro Lys Arg Ile Ala Lys AlaVal Asn Glu Lys Ser 325 330 335 Cys Asn Cys Leu Leu Leu Lys Val Asn GlnIle Gly Ser Val Thr Glu 340 345 350 Ser Leu Gln Ala Cys Lys Leu Ala GlnAla Asn Gly Trp Gly Val Met 355 360 365 Val Ser His Arg Ser Gly Glu ThrGlu Asp Thr Phe Ile Ala Asp Leu 370 375 380 Val Val Gly Leu Cys Thr GlyGln Ile Lys Thr Gly Ala Pro Cys Arg 385 390 395 400 Ser Glu Arg Leu AlaLys Tyr Asn Gln Leu Leu Arg Ile Glu Glu Glu 405 410 415 Leu Gly Ser LysAla Lys Phe Ala Gly Arg Asn Phe Arg Asn Pro Leu 420 425 430 Ala Lys 271755 DNA Homo sapiens Alpha-enolase 27 acggagatct cgccggcttt acgttcacctcggtgtctgc agcaccctcc gcttcctctc 60 ctaggcgacg agacccagtg gctagaagttcaccatgtct attctcaaga tccatgccag 120 ggagatcttt gactctcgcg ggaatcccactgttgaggtt gatctcttca cctcaaaagg 180 tctcttcaga gctgctgtgc ccagtggtgcttcaactggt atctatgagg ccctagagct 240 ccgggacaat gataagactc gctatatggggaagggtgtc tcaaaggctg ttgagcacat 300 caataaaact attgcgcctg ccctggttagcaagaaactg aacgtcacag aacaagagaa 360 gattgacaaa ctgatgatcg agatggatggaacagaaaat aaatctaagt ttggtgcgaa 420 cgccattctg ggggtgtccc ttgccgtctgcaaagctggt gccgttgaga agggggtccc 480 cctgtaccgc cacatcgctg acttggctggcaactctgaa gtcatcctgc cagtcccggc 540 gttcaatgtc atcaatggcg gttctcatgctggcaacaag ctggccatgc aggagttcat 600 gatcctccca gtcggtgcag caaacttcagggaagccatg cgcattggag cagaggttta 660 ccacaacctg aagaatgtca tcaaggagaaatatgggaaa gatgccacca atgtggggga 720 tgaaggcggg tttgctccca acatcctggagaataaagaa ggcctggagc tgctgaagac 780 tgctattggg aaagctggct acactgataaggtggtcatc ggcatggacg tagcggcctc 840 cgagttcttc aggtctggga agtatgacctggacttcaag tctcccgatg accccagcag 900 gtacatctcg cctgaccagc tggctgacctgtacaagtcc ttcatcaagg actacccagt 960 ggtgtctatc gaagatccct ttgaccaggatgactgggga gcttggcaga agttcacagc 1020 cagtgcagga atccaggtag tgggggatgatctcacagtg accaacccaa agaggatcgc 1080 caaggccgtg aacgagaagt cctgcaactgcctcctgctc aaagtcaacc agattggctc 1140 cgtgaccgag tctcttcagg cgtgcaagctggcccaggcc aatggttggg gcgtcatggt 1200 gtctcatcgt tcgggggaga ctgaagataccttcatcgct gacctggttg tggggctgtg 1260 cactgggcag atcaagactg gtgccccttgccgatctgag cgcttggcca agtacaacca 1320 gctcctcaga attgaagagg agctgggcagcaaggctaag tttgccggca ggaacttcag 1380 aaaccccttg gccaagtaag ctgtgggcaggcaagccttc ggtcacctgt tggctacaca 1440 gacccctccc ctcgtgtcag ctcaggcagctcgaggcccc cgaccaacac ttgcaggggt 1500 ccctgctagt tagcgcccca ccgccgtggagttcgtaccg cttccttaga acttctacag 1560 aagccaagct ccctggagcc ctgttggcagctctagcttt tgcagtcgtg taatgggccc 1620 aagtcattgt ttttctcgcc tcactttccaccaagtgtct agagtcatgt gagcctcgtg 1680 tcatctccgg ggtggccaca ggctagatccccggtggttt tgtgctcaaa ataaaaagcc 1740 tcagtgaccc atgag 1755 28 316 PRTRattus norvegicus Aldose reductase 28 Met Ala Ser His Leu Glu Leu AsnAsn Gly Thr Lys Met Pro Thr Leu 1 5 10 15 Gly Leu Gly Thr Trp Lys SerPro Pro Gly Gln Val Thr Glu Ala Val 20 25 30 Lys Val Ala Ile Asp Met GlyTyr Arg His Ile Asp Cys Ala Gln Val 35 40 45 Tyr Gln Asn Glu Lys Glu ValGly Val Ala Leu Gln Glu Lys Leu Lys 50 55 60 Glu Gln Val Val Lys Arg GlnAsp Leu Phe Ile Val Ser Lys Leu Trp 65 70 75 80 Cys Thr Phe His Asp GlnSer Met Val Lys Gly Ala Cys Gln Lys Thr 85 90 95 Leu Ser Asp Leu Gln LeuAsp Tyr Leu Asp Leu Tyr Leu Ile His Trp 100 105 110 Pro Thr Gly Phe LysPro Gly Pro Asp Tyr Phe Pro Leu Asp Ala Ser 115 120 125 Gly Asn Val IlePro Ser Asp Thr Asp Phe Val Asp Thr Trp Thr Ala 130 135 140 Met Glu GlnLeu Val Asp Glu Gly Leu Val Lys Ala Ile Gly Val Ser 145 150 155 160 AsnPhe Asn Pro Leu Gln Ile Glu Arg Ile Leu Asn Lys Pro Gly Leu 165 170 175Lys Tyr Lys Pro Ala Val Asn Gln Ile Glu Cys His Pro Tyr Leu Thr 180 185190 Gln Glu Lys Leu Ile Glu Tyr Cys His Cys Lys Gly Ile Val Val Thr 195200 205 Ala Tyr Ser Pro Leu Gly Ser Pro Asp Arg Pro Trp Ala Lys Pro Glu210 215 220 Asp Pro Ser Leu Leu Glu Asp Pro Arg Ile Lys Glu Ile Ala AlaLys 225 230 235 240 Tyr Asn Lys Thr Thr Ala Gln Val Leu Ile Arg Phe ProIle Gln Arg 245 250 255 Asn Leu Val Val Ile Pro Lys Ser Val Thr Pro AlaArg Ile Ala Glu 260 265 270 Asn Phe Lys Val Phe Asp Phe Glu Leu Ser AsnGlu Asp Met Ala Thr 275 280 285 Leu Leu Ser Tyr Asn Arg Asn Trp Arg ValCys Ala Leu Met Ser Cys 290 295 300 Ala Lys His Lys Asp Tyr Pro Phe HisAla Glu Val 305 310 315 29 1337 DNA Rattus norvegicus Aldose reductase29 ctcttgcggg tcgttgtgcg taacttgcag caatcatggc tagccatctg gaactcaaca 60acggcaccaa gatgcccacc ctgggtctgg gcacctggaa gtctcctcct ggccaggtga 120ccgaggctgt gaaggttgct atcgacatgg ggtatcgcca cattgactgc gcccaggtgt 180accagaatga gaaggaggtg ggggtggccc tccaggagaa gctcaaggag caggtggtga 240agcgccagga tctcttcatt gtcagcaagc tgtggtgcac gttccacgac cagagcatgg 300tgaaaggggc ctgccagaag acgctgagcg acctgcagct ggactacctg gacctctacc 360ttattcactg gccaactggc ttcaagcctg ggcctgacta tttccccctg gatgcatcgg 420gaaacgtgat tcctagtgac accgattttg tggacacttg gacggctatg gagcaactag 480tggatgaagg tttggtaaaa gcaatcggag tctccaactt caaccctctt cagattgaga 540ggatcttgaa caaacctggc ttaaagtata agcctgctgt taaccagatc gagtgccacc 600catacctaac tcaggagaag ctgattgagt actgccattg caaaggcatc gtggtgactg 660catacagtcc ccttggttct cctgacaggc cctgggccaa gcctgaggac ccctctctcc 720tggaggatcc caggatcaag gaaattgcag ccaagtacaa taaaactaca gcccaggtgc 780tgatccggtt ccccatccaa aggaacctgg tcgtgatccc caagtctgtg acaccagcac 840gtattgctga gaactttaag gtctttgact ttgagctgag caatgaggac atggccactc 900tactcagcta caacaggaac tggagggtgt gcgccttgat gagctgtgcc aaacacaagg 960attacccctt ccacgcagaa gtctgaagct gtggtggacg aatcctgctc ctccccaagc 1020gacttaacac atgttctttc tgcctcatct gcccttgcaa gtgtccctct gcactgggtg 1080gcaccttgca gaccagatgg tgagagtttg ttagtttgac gtagaatgtg gagggcagta 1140ccagtagctg aggagtttct tcggcctttc ttggtcttct tcccacctgg aggactttaa 1200cacgagtacc ttttccaacc aaagagaaag caagatttat agcccaagtc atgccactaa 1260cacttaaatt tgagtgctta gaactccagt cctatggggg tcagactttt tgcctcaaat 1320aaaaactgct tttgtcg 1337 30 299 PRT Rattus norvegicus Cytochrome Coxidase, subunit 1 30 Glu Phe Pro Phe Phe Asp Pro Ala Gly Gly Gly AspPro Ile Leu Tyr 1 5 10 15 Gln His Leu Phe Trp Phe Phe Gly His Pro GluVal Tyr Ile Leu Ile 20 25 30 Leu Pro Gly Phe Gly Ile Ile Ser His Val ValThr Tyr Tyr Ser Gly 35 40 45 Lys Lys Glu Pro Phe Gly Tyr Met Gly Met ValTrp Ala Met Met Ser 50 55 60 Ile Gly Phe Leu Gly Phe Ile Val Trp Ala HisHis Met Phe Thr Val 65 70 75 80 Gly Leu Asp Val Asp Thr Arg Ala Tyr PheThr Ser Ala Thr Met Ile 85 90 95 Ile Ala Ile Pro Thr Gly Val Lys Val PheSer Trp Leu Ala Thr Leu 100 105 110 His Gly Gly Asn Ile Lys Trp Ser ProAla Met Leu Trp Ala Leu Gly 115 120 125 Phe Ile Phe Leu Phe Thr Val GlyGly Leu Thr Gly Ile Val Leu Ser 130 135 140 Asn Ser Ser Leu Asp Ile ValLeu His Asp Thr Tyr Tyr Val Val Ala 145 150 155 160 His Phe His Tyr ValLeu Ser Met Gly Ala Val Phe Ala Ile Met Ala 165 170 175 Cys Phe Val HisTrp Phe Pro Leu Phe Ser Gly Tyr Thr Leu Asn Asp 180 185 190 Thr Trp AlaLys Ala His Phe Ala Ile Met Phe Val Gly Val Asn Met 195 200 205 Thr PhePhe Pro Gln His Phe Leu Gly Leu Ala Gly Met Pro Arg Arg 210 215 220 TyrSer Asp Tyr Pro Asp Ala Tyr Thr Thr Trp Asn Thr Val Ser Ser 225 230 235240 Met Gly Ser Phe Ile Ser Leu Thr Ala Val Leu Val Met Ile Phe Met 245250 255 Ile Trp Glu Ala Phe Ala Ser Lys Arg Glu Val Leu Ser Ile Ser Tyr260 265 270 Ser Ser Thr Asn Leu Glu Trp Leu His Gly Cys Pro Pro Pro TyrHis 275 280 285 Thr Phe Glu Glu Pro Ser Tyr Val Lys Val Lys 290 295 31987 DNA Rattus norvegicus Cytochrome C oxidase, subunit 1 31 gaattccctttcttcgaccc cgctggaggt ggagacccaa ttctttatca acacctattc 60 tgattcttcggccacccaga agtgtacatc ttaattcttc cagggtttgg aattatttca 120 catgtagttacctattactc tggaaaaaaa gaacccttcg gatatatagg tatggtatga 180 gccataatatctattggctt cctaggattt attgtatgag cacatcacat attcacagta 240 ggcctagatgtagacacccg agcctacttt acatctgcca ctataattat cgcaattcct 300 acaggcgtaaaagtattcag ctgactcgct acactacatg gaggaaatat caaatgatcc 360 cccgccatattatgagcctt agggtttatc ttcttattca cagtaggggg cctaacaggg 420 atcgtactatctaactcatc ccttgacatt gtacttcatg atacatacta cgtagtagct 480 cacttccactatgtcttatc tataggagca gtattcgcca tcatagcttg cttcgtccac 540 tgattcccactattctcagg ctatacccta aatgacacat gagcaaaagc ccactttgcc 600 attatatttgtaggtgtaaa cataacattt tttcctcaac acttcctagg attagcaggg 660 atacctcgtcgttactctga ttatccagat gcttatacca catgaaatac agtctcctct 720 ataggctcattcatctcact tacggccgtc cttgtaatga tcttcatgat ttgagaagcc 780 ttcgcatcaaaacgagaagt gctctcaatt tcctactctt caactaacct agaatgactg 840 catggatgccccccacctta ccacacattc gaagaacctt cctatgtaaa agttaaataa 900 gaaaggaaggattcgaaccc cctacaactg gtttcaagcc aatttcataa ccattatgtc 960 tttctcaataaaaaaaaaaa ggaattc 987 32 334 PRT Rattus norvegicus LDH-B 32 Met Ala ThrLeu Lys Glu Lys Leu Ile Ala Pro Val Ala Asp Asp Glu 1 5 10 15 Thr AlaVal Pro Asn Asn Lys Ile Thr Val Val Gly Val Gly Gln Val 20 25 30 Gly MetAla Cys Ala Ile Ser Ile Leu Gly Lys Ser Leu Ala Asp Glu 35 40 45 Leu AlaLeu Val Asp Val Leu Glu Asp Lys Leu Lys Gly Glu Met Met 50 55 60 Asp LeuGln His Gly Ser Leu Phe Leu Gln Thr Pro Lys Ile Val Ala 65 70 75 80 AspLys Asp Tyr Ser Val Thr Ala Asn Ser Lys Ile Val Val Val Thr 85 90 95 AlaGly Val Arg Gln Gln Glu Gly Glu Ser Arg Leu Asn Leu Val Gln 100 105 110Arg Asn Val Asn Val Phe Lys Phe Ile Ile Pro Gln Ile Val Lys Tyr 115 120125 Ser Pro Asp Cys Thr Ile Ile Val Val Ser Asn Pro Val Asp Ile Leu 130135 140 Thr Tyr Val Thr Trp Lys Leu Ser Gly Leu Pro Lys His Arg Val Ile145 150 155 160 Gly Ser Gly Cys Asn Leu Asp Ser Ala Arg Phe Arg Tyr LeuMet Ala 165 170 175 Glu Lys Leu Gly Ile His Pro Ser Ser Cys His Gly TrpIle Leu Gly 180 185 190 Glu His Gly Asp Ser Ser Val Ala Val Trp Ser GlyVal Asn Val Ala 195 200 205 Gly Val Ser Leu Gln Glu Leu Asn Pro Glu MetGly Thr Asp Asn Asp 210 215 220 Ser Glu Asn Trp Lys Glu Val His Lys MetVal Val Asp Ser Ala Tyr 225 230 235 240 Glu Val Ile Lys Leu Lys Gly TyrThr Asn Trp Ala Ile Gly Leu Ser 245 250 255 Val Ala Asp Leu Ile Glu SerMet Leu Lys Asn Leu Ser Arg Ile His 260 265 270 Pro Val Ser Thr Met ValLys Gly Met Tyr Gly Ile Glu Asn Glu Val 275 280 285 Phe Leu Ser Leu ProCys Ile Leu Asn Ala Arg Gly Leu Thr Ser Val 290 295 300 Ile Asn Gln LysLeu Lys Asp Asp Glu Val Ala Gln Leu Arg Lys Ser 305 310 315 320 Ala AspThr Leu Trp Asp Ile Gln Lys Asp Leu Lys Asp Leu 325 330 33 1217 DNARattus norvegicus LDH-B 33 cagagcctcc tcttgtctgg acaagatggc aacccttaaggaaaagctca ttgcgccagt 60 cgcagacgac gagactgccg tcccgaacaa caagattactgtagtaggcg ttggacaagt 120 tggaatggct tgtgctatca gcattctggg gaagtctctggctgatgagc ttgccctggt 180 ggatgtcttg gaagacaagc tcaaaggaga aatgatggatctgcagcacg ggagcttatt 240 tctccagact ccgaaaatcg tggctgataa agattactccgtgacagcca attctaagat 300 tgtggtggtg accgcgggag tccgccagca ggagggggagagtcggctca acctggtgca 360 gagaaacgtc aatgtattca agttcattat tcctcagatcgtcaagtaca gccccgactg 420 caccatcatc gtggtttcca acccagtgga tattcttacctatgtcacct ggaagctgag 480 cgggctacct aagcaccgcg tgattggaag tggatgcaatctggattctg ctcggtttcg 540 ttacctcatg gccgaaaagc ttggtattca tcccagcagctgccacggct ggatcctggg 600 cgagcacggg gactccagtg tggcagtgtg gagcggggtgaatgtggcag gagtctccct 660 ccaggaactg aacccagaga tgggaacgga caatgacagcgagaactgga aggaggtgca 720 taagatggtg gtggacagtg cctatgaagt catcaagctaaaaggctaca ccaactgggc 780 catcggccta agtgtggctg acctcatcga atccatgctgaaaaacctct ctcggattca 840 ccccgtgtct acaatggtga agggaatgta cggcatcgagaacgaagtct tcctcagtct 900 cccgtgcatc cttaatgctc ggggactgac cagcgtcatcaaccagaagc tgaaggacga 960 tgaggtcgct cagctcagga agagtgcgga caccctgtgggatatccaga aagacctcaa 1020 ggacctgtga ctgccaggcg ccaggctgta gaaatccaaacctccaatgt gactaagtga 1080 acctttagtc ttcggccttg tacgtaggtc acagtttgcttcttccctaa catgtgataa 1140 tgagctcaca gatcaaaacc aggagtgttt gatgtttgcactaggagctc ctgaacaaat 1200 aaagtttagc aattgca 1217 34 86 PRT Homosapiens Cytochrome C oxidase subunit VIb 34 Met Ala Glu Asp Met Glu ThrLys Ile Lys Asn Tyr Lys Thr Ala Pro 1 5 10 15 Phe Asp Ser Arg Phe ProAsn Gln Asn Gln Thr Arg Asn Cys Trp Gln 20 25 30 Asn Tyr Leu Asp Phe HisArg Cys Gln Lys Ala Met Thr Ala Lys Gly 35 40 45 Gly Asp Ile Ser Val CysGlu Trp Tyr Gln Arg Val Tyr Gln Ser Leu 50 55 60 Cys Pro Thr Ser Trp ValThr Asp Trp Asp Glu Gln Arg Ala Glu Gly 65 70 75 80 Thr Phe Pro Gly LysIle 85 35 439 DNA Homo sapiens Cytochrome C oxidase subunit VIb 35ttgagctgca ggttgaatcc ggggtgcctt taggattcag caccatggcg gaagacatgg 60agaccaaaat caagaactac aagaccgccc cttttgacag ccgcttcccc aaccagaacc 120agactagaaa ctgctggcag aactacctgg acttccaccg ctgtcagaag gcaatgaccg 180ctaaaggagg cgatatctct gtgtgcgaat ggtaccagcg tgtgtaccag tccctctgcc 240ccacatcctg ggtcacagac tgggatgagc aacgggctga aggcacgttt cccgggaaga 300tctgaactgg ctgcatctcc ctttcctctg tcctccatcc ttctcccagg atggtgaagg 360gggacctggt acccagtgat ccccacccca ggatcctaaa tcatgactta cctgctaata 420aaaactcatt ggaaaagtg 439 36 172 PRT Homo sapiens NADH ubiquinoneoxidoreductase PGIV subunit 36 Met Pro Gly Ile Val Glu Leu Pro Thr LeuGlu Glu Leu Lys Val Asp 1 5 10 15 Glu Val Lys Ile Ser Ser Ala Val LeuLys Ala Ala Ala His His Tyr 20 25 30 Gly Ala Gln Cys Asp Lys Pro Asn LysGlu Phe Met Leu Cys Arg Trp 35 40 45 Glu Glu Lys Asp Pro Arg Arg Cys LeuGlu Glu Gly Lys Leu Val Asn 50 55 60 Lys Cys Ala Leu Asp Phe Phe Arg GlnIle Lys Arg His Cys Ala Glu 65 70 75 80 Pro Phe Thr Glu Tyr Trp Thr CysIle Asp Tyr Thr Gly Gln Gln Leu 85 90 95 Phe Arg His Cys Arg Lys Gln GlnAla Lys Phe Asp Glu Cys Val Leu 100 105 110 Asp Lys Leu Gly Trp Val ArgPro Asp Leu Gly Glu Leu Ser Lys Val 115 120 125 Thr Lys Val Lys Thr AspArg Pro Leu Pro Glu Asn Pro Tyr His Ser 130 135 140 Arg Pro Arg Pro AspPro Ser Pro Glu Ile Glu Gly Asp Leu Gln Pro 145 150 155 160 Ala Thr HisGly Ser Arg Phe Tyr Phe Trp Thr Lys 165 170 37 700 DNA Homo sapiens NADHubiquinone oxidoreductase PGIV subunit 37 ggggagttca aggagacgggggcgacgcgg ctgagggctt ctcgtcgggg tcggggctgc 60 agccgtcatg ccggggatagtggagctgcc cactctagag gagctgaaag tagatgaggt 120 gaaaattagt tctgctgtgcttaaagctgc ggcccatcac tatggagctc aatgtgataa 180 gcccaacaag gaatttatgctctgccgctg ggaagagaaa gatccgaggc ggtgtttaga 240 ggaaggcaaa ctggtcaacaagtgtgcttt ggacttcttt aggcagataa aacgtcactg 300 tgcagagcct tttacagaatattggacttg cattgattat actggccagc agttatttcg 360 tcactgtcgc aaacagcaggcaaagtttga cgagtgtgtg ctggacaaac tgggctgggt 420 gcggcctgac ctgggagaactgtcaaaggt caccaaagtg aaaacagatc gacctttacc 480 ggagaatccc tatcactcaagaccaagacc ggatcccagc cctgagatcg agggagatct 540 gcagcctgcc acacatggcagccgctttta tttctggacc aagtaaagat gggtccgtgg 600 cccacactcg gtcatgtgctcagacaacga ctgatgaaaa cgcccatgcg gtttgcatcg 660 actgatagtg tgttctttccgggatcacaa acattaacaa 700 38 532 PRT Mus musculus succinatedehydrogenase Fp subunit 38 Leu Arg Ala Ala Phe Gly Leu Ser Glu Ala GlyPhe Asn Thr Ala Cys 1 5 10 15 Leu Thr Lys Leu Phe Pro Thr Arg Ser HisThr Val Ala Ala Gln Gly 20 25 30 Gly Ile Asn Ala Ala Leu Gly Asn Met GluGlu Asp Asn Trp Arg Trp 35 40 45 His Phe Tyr Asp Thr Val Lys Gly Ser AspTrp Leu Gly Asp Gln Asp 50 55 60 Ala Ile His Tyr Met Thr Glu Gln Ala ProAla Ser Val Val Glu Leu 65 70 75 80 Glu Asn Tyr Gly Met Pro Phe Ser ArgThr Glu Asp Gly Lys Ile Tyr 85 90 95 Gln Arg Ala Phe Gly Gly Gln Ser LeuLys Phe Gly Lys Gly Gly Gln 100 105 110 Ala His Arg Cys Cys Cys Val AlaAsp Arg Thr Gly His Ser Leu Leu 115 120 125 His Thr Leu Tyr Gly Arg SerLeu Arg Tyr Asp Thr Ser Tyr Phe Val 130 135 140 Glu Tyr Phe Ala Leu AspLeu Leu Met Glu Asn Gly Glu Cys Arg Gly 145 150 155 160 Val Ile Ala LeuCys Ile Glu Asp Gly Ser Ile His Arg Ile Arg Ala 165 170 175 Lys Asn ThrVal Ile Ala Thr Gly Gly Tyr Gly Arg Thr Tyr Phe Ser 180 185 190 Cys ThrSer Ala His Thr Ser Thr Gly Asp Gly Thr Ala Met Val Thr 195 200 205 ArgAla Gly Leu Pro Cys Gln Asp Leu Glu Phe Val Gln Phe His Pro 210 215 220Thr Gly Ile Tyr Gly Ala Gly Cys Leu Ile Thr Glu Gly Cys Arg Gly 225 230235 240 Glu Gly Gly Ile Leu Ile Asn Ser Gln Gly Glu Arg Phe Met Glu Arg245 250 255 Tyr Ala Pro Val Ala Lys Asp Leu Ala Ser Arg Asp Val Val SerArg 260 265 270 Ser Met Thr Leu Glu Ile Arg Glu Gly Arg Gly Cys Gly ProGlu Lys 275 280 285 Asp His Val Tyr Leu Gln Leu His His Leu Pro Pro GluGln Leu Ala 290 295 300 Thr Arg Leu Pro Gly Ile Ser Glu Thr Ala Met IlePhe Ala Gly Val 305 310 315 320 Asp Val Thr Lys Glu Pro Ile Pro Val LeuPro Thr Val His Tyr Asn 325 330 335 Met Gly Gly Ile Pro Thr Asn Tyr LysGly Gln Val Leu Lys His Val 340 345 350 Asn Gly Gln Asp Gln Ile Val ProGly Leu Tyr Ala Cys Gly Glu Ala 355 360 365 Ala Cys Ala Ser Val His GlyAla Asn Arg Leu Gly Ala Asn Ser Leu 370 375 380 Leu Asp Leu Val Val PheGly Arg Ala Cys Ala Leu Ser Ile Ala Glu 385 390 395 400 Ser Cys Arg ProGly Asp Lys Val Pro Ser Ile Lys Ala Asn Ala Gly 405 410 415 Glu Glu SerVal Met Asn Leu Asp Lys Leu Arg Phe Ala Asp Gly Ser 420 425 430 Ile ArgThr Ser Glu Leu Arg Leu Asn Met Gln Lys Ser Met Gln Asn 435 440 445 HisAla Ala Val Phe Arg Val Gly Ser Val Leu Gln Glu Gly Cys Glu 450 455 460Lys Ile Ser Gln Leu Tyr Gly Asp Leu Lys His Leu Lys Thr Phe Asp 465 470475 480 Arg Gly Met Val Trp Asn Thr Asp Leu Val Glu Thr Leu Glu Leu Gln485 490 495 Asn Leu Met Leu Cys Ala Leu Gln Thr Ile Tyr Gly Ala Glu AlaArg 500 505 510 Lys Glu Ser Arg Gly Ala His Ala Arg Glu Asp Tyr Lys ValArg Val 515 520 525 Asp Glu Tyr Asp 530 39 1596 DNA Mus musculussuccinate dehydrogenase Fp subunit 39 ttgcgagctg catttggcct ttctgaggcagggtttaata ctgcatgcct tacaaagctc 60 tttcctaccc gatcacatac tgttgcagcacagggaggta tcaatgctgc tctggggaac 120 atggaagagg acaactggag atggcatttctatgacactg tgaaaggctc cgactggctg 180 ggggatcagg atgccatcca ttacatgacagagcaagctc ctgcctctgt ggttgagcta 240 gaaaattatg gtatgccatt tagcagaactgaagatggga agatttatca gcgtgcattt 300 ggtggacaga gcctcaagtt tgggaaaggcgggcaggctc atcggtgttg ctgtgtggct 360 gatcggacag gccactcact cttacacaccttgtatggaa gatctctgcg atatgacacc 420 agttattttg tggaatattt tgcactggatcttctgatgg aaaatgggga gtgccgtggt 480 gtcattgcac tgtgcataga agatgggtccatacaccgaa taagagcaaa aaacactgtt 540 attgctactg ggggctacgg gcgaacctacttcagctgta catctgccca taccagcaca 600 ggggacggca cagccatggt cactagggctggtttgcctt gccaggactt agaatttgtt 660 cagttccacc ccacaggtat atacggtgctggctgcctca tcacagaagg gtgtcgtgga 720 gaggggggga ttctcatcaa cagtcaaggtgaaaggttca tggagagata cgcccctgtt 780 gccaaggacc tggcatcaag agatgttgtgtctcgatcca tgactcttga gatccgtgaa 840 ggaagaggct gtggccctga gaaagatcacgtctacctgc agttgcatca tctgcctcct 900 gagcaactgg ccacacgtct gcccggaatttcagagacag ccatgatctt cgctggtgtg 960 gatgtcacta aggagcccat tccagtcctccccactgtgc attacaacat gggtgggatt 1020 cccactaact acaagggaca ggtgctgaagcatgtgaatg gccaggatca gattgtgcct 1080 ggactgtatg cctgtgggga ggctgcctgcgcctcagtgc atggtgccaa ccggcttgga 1140 gcaaattctc tcttggacct tgtagtctttggcagagcct gtgccctgag cattgcagaa 1200 tcttgcaggc ctggagataa agttccttcaattaaggcaa atgctggaga agaatcggtt 1260 atgaatcttg acaagttgag atttgccgatggaagtataa gaacatcaga actacgccta 1320 aacatgcaga agtcgatgca gaaccatgctgcagtgttcc gtgtggggag tgtattgcaa 1380 gaaggctgtg aaaaaatcag tcagctctatggagacctaa agcatctaaa gacattcgac 1440 aggggaatgg tttggaacac agacctggtggagaccctgg agctgcagaa tctgatgctg 1500 tgcgcactgc agaccatata tggtgcagaagctcggaagg agtcccgggg agcccatgcc 1560 agggaagatt acaaagtgcg ggtcgatgagtatgat 1596 40 453 PRT Homo sapiens Core protein II of humanmitochondrial cytochrome bc-1 complex 40 Met Lys Leu Leu Thr Arg Ala GlySer Phe Ser Arg Phe Tyr Ser Leu 1 5 10 15 Lys Val Ala Pro Lys Val LysAla Thr Ala Ala Pro Ala Gly Ala Pro 20 25 30 Pro Gln Pro Gln Asp Leu GluPhe Thr Lys Leu Pro Asn Gly Leu Val 35 40 45 Ile Ala Ser Leu Glu Asn TyrSer Pro Val Ser Arg Ile Gly Leu Phe 50 55 60 Ile Lys Ala Gly Ser Arg TyrGlu Asp Phe Ser Asn Leu Gly Thr Thr 65 70 75 80 His Leu Leu Arg Leu ThrSer Ser Leu Thr Thr Lys Gly Ala Ser Ser 85 90 95 Phe Lys Ile Thr Arg GlyIle Glu Ala Val Gly Gly Lys Leu Ser Val 100 105 110 Thr Ala Thr Arg GluAsn Met Ala Tyr Thr Val Glu Cys Leu Arg Gly 115 120 125 Asp Val Asp IleLeu Met Glu Phe Leu Leu Asn Val Thr Thr Ala Pro 130 135 140 Glu Phe ArgArg Trp Glu Val Ala Asp Leu Gln Pro Gln Leu Lys Ile 145 150 155 160 AspLys Ala Val Ala Phe Gln Asn Pro Gln Thr His Val Ile Glu Asn 165 170 175Leu His Ala Ala Ala Tyr Gln Asn Ala Leu Ala Asn Pro Leu Tyr Cys 180 185190 Pro Asp Tyr Arg Ile Gly Lys Val Thr Ser Glu Glu Leu His Tyr Phe 195200 205 Val Gln Asn His Phe Thr Ser Ala Arg Met Ala Leu Ile Gly Leu Gly210 215 220 Val Ser His Pro Val Leu Lys Gln Val Ala Glu Gln Phe Leu AsnMet 225 230 235 240 Arg Gly Gly Leu Gly Leu Ser Gly Ala Lys Ala Asn TyrArg Gly Gly 245 250 255 Glu Ile Arg Glu Gln Asn Gly Asp Ser Leu Val HisAla Ala Phe Val 260 265 270 Ala Glu Ser Ala Val Ala Gly Ser Ala Glu AlaAsn Ala Phe Ser Val 275 280 285 Leu Gln His Val Leu Gly Ala Gly Pro HisVal Lys Arg Gly Ser Asn 290 295 300 Thr Thr Ser His Leu His Gln Ala ValAla Lys Ala Thr Gln Gln Pro 305 310 315 320 Phe Asp Val Ser Ala Phe AsnAla Ser Tyr Ser Asp Ser Gly Leu Phe 325 330 335 Gly Ile Tyr Thr Ile SerGln Ala Thr Ala Ala Gly Asp Val Ile Lys 340 345 350 Ala Ala Tyr Asn GlnVal Lys Arg Ile Ala Gln Gly Asn Leu Ser Asn 355 360 365 Thr Asp Val GlnAla Ala Lys Asn Lys Leu Lys Ala Gly Tyr Leu Met 370 375 380 Ser Val GluSer Ser Glu Cys Phe Leu Glu Glu Val Gly Ser Gln Ala 385 390 395 400 LeuVal Ala Gly Ser Tyr Met Pro Pro Ser Thr Val Leu Gln Gln Ile 405 410 415Asp Ser Val Ala Asn Ala Asp Ile Ile Asn Ala Ala Lys Lys Phe Val 420 425430 Ser Gly Gln Lys Ser Met Ala Ala Ser Gly Asn Leu Gly His Thr Pro 435440 445 Phe Val Asp Glu Leu 450 41 1588 DNA Homo sapiens Core protein IIof human mitochondrial cytochrome bc-1 complex 41 atcttgcttt cctttaatccggcagtgacc gtgtgtcaga acaatcttga atcatgaagc 60 tactaaccag agccggctctttctcgagat tttattccct caaagttgcc cccaaagtta 120 aagccacagc tgcgcctgcaggagcaccgc cacaacctca ggaccttgag tttaccaagt 180 taccaaatgg cttggtgattgcttctttgg aaaactattc tcctgtatca agaattggtt 240 tgttcattaa agcaggcagtagatatgagg acttcagcaa tttaggaacc acccatttgc 300 tgcgtcttac atccagtctgacgacaaaag gagcttcatc tttcaagata acccgtggaa 360 ttgaagcagt tggtggcaaattaagtgtga ccgcaacaag ggaaaacatg gcttatactg 420 tggaatgcct gcggggtgatgttgatattc taatggagtt cctgctcaat gtcaccacag 480 caccagaatt tcgtcgttgggaagtagctg accttcagcc tcagctaaag attgacaaag 540 ctgtggcctt tcagaatccgcagactcatg tcattgaaaa tttgcatgca gcagcttacc 600 agaatgcctt ggctaatcccttgtattgtc ctgactatag gattggaaaa gtgacatcag 660 aggagttaca ttacttcgttcagaaccatt tcacaagtgc aagaatggct ttgattggac 720 ttggtgtgag tcatcctgttctaaagcaag ttgctgaaca gtttctcaac atgaggggtg 780 ggcttggttt atctggtgcaaaggccaact accgtggagg tgaaatccga gaacagaatg 840 gagacagtct tgtccatgctgcttttgtag cagaaagtgc tgtcgcggga agtgcagagg 900 caaatgcatt tagtgttcttcagcatgtcc tcggtgctgg gccacatgtc aagaggggca 960 gcaacaccac cagccatctgcaccaggctg ttgccaaggc aactcagcag ccatttgatg 1020 tttctgcatt taatgccagttactcagatt ctggactctt tgggatttat actatctccc 1080 aggccacagc tgctggagatgttatcaagg ctgcctataa tcaagtaaaa agaatagctc 1140 aaggaaacct ttccaacacagatgtccaag ctgccaagaa caagctgaaa gctggatacc 1200 taatgtcagt ggagtcttctgagtgtttcc tggaagaagt cgggtcccag gctctagttg 1260 ctggttctta catgccaccatccacagtcc ttcagcagat tgattcagtg gctaatgctg 1320 atatcataaa tgcggcaaagaagtttgttt ctggccagaa gtcaatggca gcaagtggaa 1380 atttgggaca tacaccttttgttgatgagt tgtaatactg atgcacacat tacaggagag 1440 agctgaacgt tctctcacccagagcagcaa acacatgaaa gtcagaagtc tctaatatat 1500 catttgtctt ttttccagtgaggtaaaata aggcataaat gcaggtaatt attcccagct 1560 gacctaaagt caataaaacattctgttt 1588 42 358 PRT Rattus norvegicus Stearoyl-CoA desaturase-2 42Met Pro Ala His Ile Leu Gln Glu Ile Ser Gly Ser Tyr Ser Ala Thr 1 5 1015 Thr Thr Ile Thr Ala Pro Pro Ser Gly Gly Gln Gln Asn Gly Gly Glu 20 2530 Lys Phe Glu Lys Asn Pro His His Trp Gly Ala Asp Val Arg Pro Glu 35 4045 Ile Lys Asp Asp Leu Tyr Asp Pro Ser Tyr Gln Asp Glu Glu Gly Pro 50 5560 Pro Pro Lys Leu Glu Tyr Val Trp Arg Asn Ile Ile Leu Met Ala Leu 65 7075 80 Leu His Ile Gly Ala Leu Tyr Gly Ile Thr Leu Val Pro Ser Cys Lys 8590 95 Val Tyr Thr Cys Leu Phe Ala Tyr Leu Tyr Tyr Val Ile Ser Ala Leu100 105 110 Gly Ile Thr Ala Gly Ala His Arg Leu Trp Ser His Arg Thr TyrLys 115 120 125 Ala Arg Leu Pro Leu Arg Leu Phe Leu Ile Ile Ala Asn ThrMet Ala 130 135 140 Phe Gln Asn Asp Val Tyr Glu Trp Ala Arg Asp His ArgAla His His 145 150 155 160 Lys Phe Ser Glu Thr His Ala Asp Pro His AsnSer Arg Arg Gly Phe 165 170 175 Phe Phe Ser His Val Gly Trp Leu Leu ValArg Lys His Pro Ala Val 180 185 190 Lys Glu Lys Gly Gly Lys Leu Asp MetSer Asp Leu Lys Ala Glu Lys 195 200 205 Leu Val Met Phe Gln Arg Arg TyrTyr Lys Pro Gly Leu Leu Leu Met 210 215 220 Cys Phe Ile Leu Pro Thr LeuVal Pro Trp Tyr Cys Trp Gly Glu Thr 225 230 235 240 Phe Val Asn Ser LeuCys Val Ser Thr Phe Leu Arg Tyr Ala Val Val 245 250 255 Leu Asn Ala ThrTrp Leu Val Asn Ser Ala Ala His Leu Tyr Gly Tyr 260 265 270 Arg Pro TyrAsp Lys Asn Ile Ser Ser Arg Glu Asn Ile Leu Val Ser 275 280 285 Met GlyAla Val Gly Glu Gly Phe His Asn Tyr His His Ala Phe Pro 290 295 300 TyrAsp Tyr Ser Ala Ser Glu Tyr Arg Trp His Ile Asn Phe Thr Thr 305 310 315320 Phe Phe Ile Asp Cys Met Ala Leu Leu Gly Leu Ala Tyr Asp Arg Lys 325330 335 Arg Val Ser Lys Ala Ala Val Leu Ala Arg Ile Lys Arg Thr Gly Glu340 345 350 Glu Ser Cys Lys Ser Gly 355 43 5055 DNA Rattus norvegicusStearoyl-CoA desaturase-2 43 ctgggctagg gatgggcaca atgcggtgtt ggtgtctgcagtattctact cctggacacc 60 ggtggcctgc aagtgcgctt ttggcgtcct ctcatttttctatccttatc tccgtccgcg 120 gctgccttgg ccagccagtt tttgattttt tatctgttccttgatcgata caaagaacct 180 aaggacatac aggacattaa tacccaactg ccagctctggcccagggctt gtactgtgca 240 gcgggctggc tgcagaaact taagtcataa cactctttgtcgctgaggtc tgaagctctc 300 tgcacgttct catccctggg aacgtgaccc cagcatccgacgccaagatg ccggctcaca 360 tactgcaaga gatctctggc tcttactcgg ccaccaccacaatcacagcg ccaccttctg 420 ggggacagca gaatggagga gagaagtttg aaaagaatcctcaccactgg ggagcagatg 480 ttcgccctga aattaaagat gacctatacg accccagctaccaggatgag gaggggcccc 540 cgcccaagct ggagtacgtc tggaggaaca tcatcctcatggccctgctg cacattggag 600 ccctgtacgg gatcacactg gtcccctcct gcaaggtctacacctgcctc tttgcatatt 660 tgtactacgt aatcagcgcc ctgggcatca cagccggggctcatcgcctg tggagccaca 720 ggacttacaa ggctcggctg cccctgaggc tctttctcatcatcgccaac accatggcat 780 tccagaacga tgtgtacgaa tgggcccggg atcaccgcgcccaccacaag ttctcagaga 840 cacacgccga ccctcacaac tcccgccgtg gctttttcttctctcacgtg ggttggctgc 900 ttgtgcgcaa acacccggct gtcaaagaga agggcggaaagctggacatg tctgacctga 960 aagctgagaa gctggtgatg ttccagagga ggtattacaagcccggcctc ctgctcatgt 1020 gcttcatcct gcccacgctg gtgccctggt actgctggggtgagacgttt gtaaacagcc 1080 tgtgcgtcag cactttctta cggtacgctg tggtgctcaacgccacctgg ctggtgaaca 1140 gcgccgccca cctctatgga tatcgcccct acgacaagaacatcagctct cgggagaaca 1200 tcctggtgtc gatgggagct gtgggcgagg gcttccacaactaccatcac gccttcccct 1260 atgactactc tgccagtgag taccgctggc acatcaacttcaccacgttc ttcatcgact 1320 gcatggccct cctgggcctg gcttatgacc ggaagagggtgtccaaggcc gctgtcttag 1380 ccaggattaa gagaactggg gaggagagct gcaagagtggctgagttcgg ggtcttccag 1440 ttcctgttcc aagagccggc ttggcagagg cttactgttctgttaattaa ctactgagta 1500 ttgctaccca gatgctaaaa tgatgaagtt aacccattctggtacagtat tgtaaaacgg 1560 acaaatatcg gaagtcaaca gctctacctt ttgatgctaagctgttctcc ccttctatct 1620 tcttttcccg ttgtcttttt cttcgctttg tccccaatgacctcacccct catcgcctcc 1680 cctaagccag cagccactcc gctgtagttc agagttcaccttccagagcg taccagcttt 1740 tcaagggtct acagtaaagg ttcacgcccc agccccaacttaacgttgct ccaagttaca 1800 gatagaagaa aaatcttgag aaatttctcc tgttattttagtccagactt tgccagatgg 1860 aatgggagaa aaaatagttt tatttggcag agagcttgcgaagtgggtaa atcttaaggg 1920 gccaaattca acactcgggc tgtgattcat caaaattgtgggagaggggg atctcctgtg 1980 atcaggctcc gctccgtctg gccgaggtgc tggcgtctcttgacacagcg tgcctcctct 2040 cctaattctg attgggtgac agccgtggaa ctttgtgacactaaaatatg gcaacacatc 2100 ggtatctcag tgtactttag gctaaagatt taaaaaaatagagaagtgaa aaacaatttg 2160 taggaaaagt ggtctttcct aaaataagat tttcttttcctttcataaca aggacctggg 2220 gccaggcgtc gtggcacccg cctcaaaaca gttgagaggagaggggcagg tgggtctctg 2280 agtttgaagc cagccagggc tagatagtga ggccttgtctcaaatagaaa agccaacaat 2340 tagcaggaca cacggagccg ggggtcctct tgcgaggcgtgtctggaatc catgagggcg 2400 gtcgctcgtg gtttttgagc ttcctgtgag ctgcacccccagacacttgt aattcctgtt 2460 cttccgtctt tcggatgcag gttgctccca caaattcaggatagctatgg cgtttccatg 2520 catcccacaa tggaaggagt gtaagacttg agcttgagtcacagctaaaa cccagaagac 2580 acaaagtcag tctgtcctgg atcctacaaa cactcaacgagtgcctccct ctggaaaaga 2640 tggtgaacac cttccttcca ctgttgggca gtggacagggaagttggagg tcgctgtagg 2700 cagctccaag gtttctaggg gcaccttgcc aggggacctcacaactttgg cggtctctag 2760 tggagcaggt taggttttag ctccctttgg ttcccgctgtgctttgtggt aaggcttttc 2820 cctgggctgt tgcctcattc ctcactagct ctgtgaccaggtaggactct gggatacatg 2880 gctgaaagct cttttgctct accacatctg cctctctttccctacccatt tttttgtgtt 2940 tcggggtggt atttttgtgg tggttgtttt gttttatttcttctttcctc tttgacgttt 3000 agtcttgctc taggcagaaa cgtcctggat atttacggtcagtaaagatg tccggtccca 3060 gcagagatac aacctctatc tgcagtaggt agagaaggctgtccctttcc ggtggctttc 3120 agattctcca cctctatccc tcccaggcct ctgatattctttaaacttta aaggaaaagt 3180 ttggccgact ttctagaaac atccctggct cagagaggctctctgtctcc tcggttgttc 3240 taaaacaaat ggtccgggtg gtgtgggtgc tggcccaagactcgattcct ctgagttagg 3300 gcagagcatg ctccctgcac tcagcaaagc ctctcgggctgatcagtgct cctgggtgat 3360 gctgtggtgc ttttaggagg aagacagtgt tggctggctggaaagcatga cagttaaggt 3420 tgtgttctta agtgcctcag tttgaaagct ttggctagggtaggagatct gagatgccgt 3480 ggactttgca gaagagattt tgtgcagcaa cgcagaagcctggctgtgtt aagattggct 3540 gtacaggata gcgaatgctg tggttggagg gcacagtcttcccgtgttag ttaaatagag 3600 aggctttagg acgtctctgc gcccacttga aggtagccttatcctttagc tgttcgctaa 3660 aatagaatcc ttgagagatt gacaatatat ttgctggcctgtcgccctat tccttggaga 3720 ggccttttga atggttttaa ttccactggt tatccggtgcccacttaatc aaatgtgaaa 3780 aataaaaagc agctctcttc ttagcctgcc cccgtggatctgaggaccct gttagataca 3840 gctgtcggta agggtgtacc agctcactaa gaaacactactgtcaagatt ttaaagctca 3900 gaccaagtga cacgttaata agaaactaag atctgattgaattttctaac agtccttgcc 3960 tcgtggacac gctaaaattc gacttgggtg ccttacctcttttctaacta gtgttactca 4020 tgggcctgcg ctcccttcct ctgcggattc cttagaaccggagagcctgt aagtgtgaca 4080 ggctcaaaac tggcctggct ggagggttag gtggactttgatctgcagtg ttccctctgg 4140 ggtcttcgtt tggggaatat ttcttggggc tgttttgctgaagtgcccgt agctgatgga 4200 tggtggaagt aatttgaacg tacgcgattt gtaggttttgttatttgttt taagatttaa 4260 aggctggttg tagcatttaa aagtgaaagc tttttcttcctggtttgcta gtgttctgcg 4320 tgtcttcttt agttgaaata ggttagtgga gaagttaagaaagcggtggc cgtgttacgc 4380 tggtggtgaa ggccagggcc gcctacaaac tctcagttttgttttgtttt gtttttttat 4440 aacagtccca ctctgacaat aatgagttca ctccttggataaggttgtta atgagggaag 4500 agcccttcag acctaatcag ctcttactgg acccagctgtcaacactgtt gcattgggga 4560 ttaagtttcc aaccccgtga atcatgggac agactcaaaccgtagcactg ggcatgtcgc 4620 ctaacaatat aatgtgactc agtattctgt gagacatgggataatattcc tgacctacct 4680 ccacgggtag gtgcgagcat gagcaaatgc tctttataaaacatttgggg atcctcagca 4740 gaggagtatt taagtcctgc acatttttat agtaaccgtccaccatggac ttgacacgtg 4800 cctatgtccc agacgacatt gttaatctac atcattcttctcagaggttg aatggatgca 4860 ttagataatg tatactcatt gctatggaaa cctgcaggactccttttcct tccttgggtg 4920 ggattttttt ttcctttatt atgtgcaaca gtagctatttgtaatctaat aattttcatt 4980 aataccaact ctgaaagtat ttctactcat ctgaggttgtgtttgttcat aataaaatga 5040 aatggatctg actgc 5055 44 605 PRT Rattusnorvegicus Ribophorin 1 44 Met Glu Ala Pro Ile Val Leu Leu Leu Leu LeuTrp Leu Ala Leu Ala 1 5 10 15 Pro Thr Pro Gly Ser Ala Ser Ser Glu AlaPro Pro Leu Val Asn Glu 20 25 30 Asp Val Lys Arg Thr Val Asp Leu Ser SerHis Leu Ala Lys Val Thr 35 40 45 Ala Glu Val Val Leu Ala His Pro Gly GlyGly Ser Thr Ala Arg Ala 50 55 60 Ser Ser Phe Val Leu Ala Leu Glu Pro GluLeu Glu Ser Arg Leu Ala 65 70 75 80 His Leu Gly Val Gln Val Lys Gly GluAsp Glu Glu Asp Asn Asn Leu 85 90 95 Glu Val Arg Glu Thr Lys Met Lys GlyLys Ser Gly Arg Phe Phe Thr 100 105 110 Val Lys Leu Pro Val Ala Leu AspPro Gly Ser Lys Ile Ser Ile Val 115 120 125 Val Glu Thr Val Tyr Thr HisVal Leu His Pro Tyr Pro Thr Gln Ile 130 135 140 Thr Gln Ser Glu Lys GlnPhe Val Val Phe Glu Gly Asn His Tyr Phe 145 150 155 160 Tyr Ser Pro TyrPro Thr Lys Thr Gln Thr Met Arg Val Arg Leu Ala 165 170 175 Ser Arg AsnVal Glu Ser His Thr Lys Leu Gly Asn Pro Ser Arg Ser 180 185 190 Glu AspIle Leu Asp Tyr Gly Pro Phe Lys Asp Ile Pro Ala Tyr Ser 195 200 205 GlnAsp Thr Phe Lys Val His Tyr Glu Asn Asn Ser Pro Phe Leu Thr 210 215 220Ile Thr Ser Met Thr Arg Val Ile Glu Val Ser His Trp Gly Asn Ile 225 230235 240 Ala Val Glu Glu Asn Val Asp Leu Lys His Thr Gly Ala Val Leu Lys245 250 255 Gly Pro Phe Ser Arg Tyr Asp Tyr Gln Arg Gln Pro Asp Ser GlyIle 260 265 270 Ser Ser Ile Arg Ser Phe Lys Thr Ile Leu Pro Ala Ala AlaGln Asp 275 280 285 Val Tyr Tyr Arg Asp Glu Ile Gly Asn Val Ser Thr SerHis Leu Leu 290 295 300 Ile Leu Asp Asp Ser Val Glu Met Glu Ile Arg ProArg Phe Gly Leu 305 310 315 320 Phe Gly Gly Trp Lys Thr His Tyr Ile ValGly Tyr Asn Leu Pro Ser 325 330 335 Tyr Glu Tyr Leu Tyr Asn Leu Gly AspGln Tyr Ala Leu Lys Met Arg 340 345 350 Phe Val Asp His Val Phe Asp GluGln Val Ile Asp Ser Leu Thr Val 355 360 365 Lys Ile Ile Leu Pro Glu GlyAla Lys Asn Ile Gln Val Asp Ser Pro 370 375 380 Tyr Asp Ile Ser Arg AlaPro Asp Glu Leu His Tyr Thr Tyr Leu Asp 385 390 395 400 Thr Phe Gly ArgPro Val Ile Val Ala Tyr Lys Lys Asn Leu Val Glu 405 410 415 Gln His IleGln Asp Ile Val Val His Tyr Thr Phe Asn Lys Val Leu 420 425 430 Met LeuGln Glu Pro Leu Leu Val Val Ala Ala Phe Tyr Ile Leu Phe 435 440 445 PheThr Val Ile Ile Tyr Val Arg Leu Asp Phe Ser Ile Thr Lys Asp 450 455 460Pro Ala Ala Glu Ala Arg Met Lys Val Ala Cys Ile Thr Glu Gln Val 465 470475 480 Leu Thr Leu Val Asn Lys Arg Leu Gly Leu Tyr Arg His Phe Asp Glu485 490 495 Thr Val Asn Arg Tyr Lys Gln Ser Arg Asp Ile Ser Thr Leu AsnSer 500 505 510 Gly Lys Lys Ser Leu Glu Thr Glu His Lys Ala Val Thr SerGlu Ile 515 520 525 Ala Val Leu Gln Ser Arg Leu Lys Thr Glu Gly Ser AspLeu Cys Asp 530 535 540 Arg Val Ser Glu Met Gln Lys Leu Asp Ala Gln ValLys Glu Leu Val 545 550 555 560 Leu Lys Ser Ala Val Glu Ala Glu Arg LeuVal Ala Gly Lys Leu Lys 565 570 575 Lys Asp Thr Tyr Ile Glu Asn Glu LysLeu Ser Ser Gly Lys Arg Gln 580 585 590 Glu Leu Val Thr Lys Ile Asp HisIle Leu Asp Ala Leu 595 600 605 45 2214 DNA Rattus norvegicus Ribophorin1 45 tcgcggtcat ggaggcgccg atcgtcttgc tgctgctgct atggctcgcc ttggccccga60 cgcctggcag cgcctcttcg gaggctccgc cgctggtcaa cgaggacgtg aagcgcacgg 120tggacctgag cagccaccta gccaaggtga cggctgaggt ggtcctggcg cacccgggcg 180gcggctctac agcacgagcc agctctttcg ttctggccct ggagcccgaa ctggagtcgc 240gacttgcaca cctaggcgtg caggtaaagg gagaagatga ggaagacaac aacctagaag 300tacgagaaac caaaatgaag gggaaaagtg ggaggttttt caccgtcaag ctcccagttg 360ctcttgatcc tgggtccaag atctcaatcg ttgtggaaac tgtctacacc catgtgcttc 420atccataccc gactcagata actcagtcag agaaacagtt tgtggtgttt gagggcaacc 480attacttcta ctctccctat ccaacaaaga cccagaccat gcgagtgaga cttgcttccc 540gaaatgtgga gagccatacc aagctgggga acccctcaag gtctgaggac atcctggact 600atgggccttt taaagacatc cctgcctaca gtcaggatac tttcaaagta cattatgaga 660acaatagccc tttcctgacc atcaccagta tgacccgggt cattgaggtt tctcactggg 720gcaatattgc tgtggaagag aacgtggact tgaagcatac aggtgcggtg ctgaagggac 780ctttctcccg ctacgattac cagaggcagc ctgacagtgg gatctcctcc attcgttctt 840ttaagaccat ccttcctgct gctgcccagg atgtgtatta ccgggatgaa atcggtaatg 900tttccaccag ccacctcctt attttggatg actccgtgga aatggaaatc cggcctcgat 960ttggtctctt tggagggtgg aagacacact acatcgttgg ttacaacctc ccaagctatg 1020agtacctcta taatctgggt gaccagtatg cactgaagat gcggtttgta gaccacgtgt 1080ttgatgagca agtgatagat tctctgacag tgaagatcat ccttcctgag ggagccaaga 1140acatccaggt agacagtccc tacgatatta gccgggcccc agatgagctg cattacacct 1200acctagacac attcggccgc ccggtgattg ttgcttacaa gaagaatcta gttgaacagc 1260acattcagga cattgtggtg cactacacat tcaacaaggt gctcatgctg caggagcctc 1320tgctggttgt ggccgccttc tacatcctgt tcttcaccgt catcatctac gtccgtctag 1380acttttccat caccaaggac ccagcagcag aagccaggat gaaagtggcc tgtatcacag 1440agcaggtctt aaccctggtc aacaagaggt taggcctcta ccgtcacttt gatgagactg 1500tcaatagata caagcagtcc cgggacatct ctaccctcaa tagtggcaag aagagcctag 1560agacagagca caaagctgtg accagtgaga ttgctgtgct gcagtctagg ctgaagacgg 1620agggctctga cctgtgtgac agggtgagcg aaatgcagaa gctggacgcg caggtcaagg 1680agctggtact gaagtctgcg gtggaggcag agaggctggt ggctggcaag ctcaagaagg 1740acacatacat cgagaatgaa aagctcagct caggaaaacg ccaggagctg gtcaccaaga 1800tcgaccacat cctagacgct ctgtagctct tgtctccaga gcaggcctgg gtggcctaca 1860cttctggatg gaataggtag agggaggaag ttgtgattag cctaagacct caagaaaaaa 1920gctgaggccc atccatggct gtgtaagaag cttgtccctt atttctgaat ggttctcttt 1980tttacaaaaa caaaaaccta aacaaaaatc ctgcccaaaa aacaaacttg gttgtgtttt 2040gagcaaaagc attttgagta gttgattgct gttttgtttt gatacctggg tattttttct 2100gtctgtaagc cctgttttat ggccttctga ttcctaatgt ttggttcttt tgtgagcgta 2160tgcatggttt tttaaaagtg tgtatgacca aataaaaata aaggagggac tgtg 2214 46 284PRT Rattus norvegicus Sulfotransferase-like protein 46 Met Ala Glu SerGlu Ala Glu Thr Pro Gly Thr Pro Gly Glu Phe Glu 1 5 10 15 Ser Lys TyrPhe Glu Phe His Gly Val Arg Leu Pro Pro Phe Cys Arg 20 25 30 Gly Lys MetGlu Asp Ile Ala Asp Phe Pro Val Arg Pro Ser Asp Val 35 40 45 Trp Ile ValThr Tyr Pro Lys Ser Gly Thr Ser Leu Leu Gln Glu Val 50 55 60 Val Tyr LeuVal Ser Gln Gly Ala Asp Pro Asp Glu Ile Gly Leu Met 65 70 75 80 Asn IleAsp Glu Gln Leu Pro Val Leu Glu Tyr Pro Gln Pro Gly Leu 85 90 95 Asp IleIle Lys Glu Leu Thr Ser Pro Arg Leu Ile Lys Ser His Leu 100 105 110 ProTyr Arg Phe Leu Pro Ser Asp Leu His Asn Gly Asp Ser Lys Val 115 120 125Ile Tyr Met Ala Arg Asn Pro Lys Asp Leu Val Val Ser Tyr Tyr Gln 130 135140 Phe His Arg Ser Leu Arg Thr Met Ser Tyr Arg Gly Thr Phe Gln Glu 145150 155 160 Phe Cys Arg Arg Phe Met Asn Asp Lys Leu Gly Tyr Gly Ser TrpPhe 165 170 175 Glu His Val Gln Glu Phe Trp Glu His Arg Met Asp Ala AsnVal Leu 180 185 190 Phe Leu Lys Tyr Glu Asp Met His Arg Asp Leu Val ThrMet Val Glu 195 200 205 Gln Leu Ala Arg Phe Leu Gly Val Ser Cys Asp LysAla Gln Leu Glu 210 215 220 Ser Leu Ile Glu His Cys His Gln Leu Val AspGln Cys Cys Asn Ala 225 230 235 240 Glu Ala Leu Pro Val Gly Arg Gly ArgVal Gly Leu Trp Lys Asp Ile 245 250 255 Phe Thr Val Ser Met Asn Glu LysPhe Asp Leu Val Tyr Lys Gln Lys 260 265 270 Met Gly Lys Cys Asp Leu ThrPhe Asp Phe Tyr Leu 275 280 47 2239 DNA Rattus norvegicusSulfotransferase-like protein 47 ggcacgagcc ctggagctgc gggccgggccgggcggcagc atggcggaga gcgaagcgga 60 gaccccgggc accccgggcg agttcgagagcaagtacttc gagttccatg gcgtgcggct 120 gcctcccttc tgccgcggga agatggaggacatcgccgac ttcccagtgc ggcccagcga 180 tgtgtggatc gtcacctacc ccaagtcaggtacgagcttg ctgcaagagg tggtctactt 240 ggtgagccag ggggctgacc ctgatgagattggcctcatg aacattgacg agcagctgcc 300 agtactggag tacccacagc ccgggctggacatcatcaag gaactgacgt ctccccgcct 360 catcaagagc caccttccct accgcttcctgccctctgac ctccacaacg gggactccaa 420 ggtcatctac atggctcgca acccaaaggacctggtggta tcttactatc agttccaccg 480 ctcgctgcgg accatgagtt accgaggaaccttccaggag ttctgtcgga ggttcatgaa 540 cgacaaattg ggctatggct cctggtttgagcacgttcag gaattctggg aacatcgaat 600 ggacgcaaat gtgcttttcc tcaagtatgaagatatgcac cgggacctgg tgaccatggt 660 ggagcagctg gccagattcc tgggtgtgtcctgtgataag gcccagctgg agtccttgat 720 cgagcactgc caccagctgg tggaccagtgctgcaatgct gaggccctgc ctgtgggccg 780 gggaagagtc gggctgtgga aggacatcttcactgtctcc atgaacgaga agtttgatct 840 agtgtataaa cagaagatgg ggaagtgtgacctcacgttt gacttttatt tataatacca 900 gaatccgcaa acttgcatgc tcacggtccccagacgctct actagctaaa agtcctgttt 960 gcgttcattt attccttgct ggacactccggaagttgctt ggaaacagca gggggcgagc 1020 ctggtggaga gagccgagga gtgagatgatgctgtctaca gcagccgcct ggccgtagcg 1080 tttgagtctc cacgtgctgg ccataccacacgcacgggca ggcaaatgtc tgcccctgta 1140 ccccatgtta ttcactgtat tttatagcgcttttcactga aaatcgaaat aaatatgtca 1200 accaaatgca agtccatttc caagggggagcaggagcgag ctgtgccggg atgctaggaa 1260 gctctcaggg gtcactgtaa tttttatagtcttctctcta ggcacagcca tcccaccttg 1320 tcccgagatc aaaggacagg ttggggggagctggagaggt gggtgtagcc tgtggagttc 1380 agttttctgg aaccatcctg acaccaagccaggtcctgtc ccaggtctct gagcccagta 1440 cagaatgttc tggaagggaa gctggcctgaggctgcggat ggcatctgaa ggcatcagtg 1500 gtgtgtgaag cgtacccaca gacaccagactgcaccaaga actagtatat ctcatagcct 1560 tcacttgctc cccaaggccg tgactggcacactcccaatg gcaagagaca tgacatacca 1620 tggttctgaa gtccctagtc atcgtcacgtctgtgccacc tgtgccaagg ctctcgctcg 1680 ggagacccag tctgcaggtt gactgtcctccatgcagata ggattggcaa gcccacctgt 1740 ctgagggggc acttagccct caaggatgtctgagggagca atggccccat gtactgtcca 1800 ccctgacacc taccggctct gtcttccagctccaatttgg ctcaagtgat gaccagatga 1860 tgggtggacc aggagattgc tgcttgtggggaccaactta ttctgttcct taccaggatg 1920 ggaagagtct taacgtggtg gggggaggctctggctgtgg aaggtgcctg ctgtgcgcca 1980 tctctgaagc aggtttggtt gtagcagcgttacctctgtc tagaggaaag cttgtgtggg 2040 tctggtgcat ggttctctga ggtgggcatggcagtgcctt tcctgtgtgt ctggggagga 2100 aggctgcttt ctgtgaaatt ttctttctatttttctattt ttagtactgt acggatgttc 2160 tggaaccaca cacggtactc tgtgcttgcttgcatcttta ataaaagaca cctcccctgt 2220 caaaaaaaaa aaaaaaaaa 2239 48 235PRT Rattus norvegicus F1-ATPase alpha subunit 48 Val Ala Asp Arg Gln MetSer Leu Leu Leu Arg Arg Pro Pro Gly Arg 1 5 10 15 Glu Ala Tyr Pro GlyAsp Val Phe Tyr Leu His Ser Arg Leu Leu Glu 20 25 30 Arg Ala Ala Lys MetAsn Asp Ser Phe Gly Gly Gly Ser Leu Thr Ala 35 40 45 Leu Pro Val Ile GluThr Gln Ala Gly Asp Val Ser Ala Tyr Ile Pro 50 55 60 Thr Asn Val Ile SerIle Thr Asp Gly Gln Ile Phe Leu Glu Thr Glu 65 70 75 80 Leu Phe Tyr LysGly Ile Arg Pro Ala Ile Asn Val Gly Leu Ser Val 85 90 95 Ser Arg Val GlySer Ala Ala Gln Thr Arg Ala Met Lys Gln Val Ala 100 105 110 Gly Thr MetLys Leu Glu Leu Ala Gln Tyr Arg Glu Val Ala Ala Phe 115 120 125 Ala GlnPhe Gly Ser Asp Leu Asp Ala Ala Thr Gln Gln Leu Leu Ser 130 135 140 ArgGly Val Arg Leu Thr Glu Leu Leu Lys Gln Gly Gln Tyr Ser Pro 145 150 155160 Met Ala Ile Glu Glu Gln Val Ala Val Ile Tyr Ala Gly Val Arg Gly 165170 175 Tyr Leu Asp Lys Leu Glu Pro Ser Lys Ile Thr Lys Phe Glu Ser Ala180 185 190 Phe Leu Ser His Val Val Ser Gln His Gln Ser Leu Leu Gly AsnIle 195 200 205 Arg Ser Asp Gly Lys Ile Ser Glu Gln Ser Asp Ala Lys LeuLys Glu 210 215 220 Ile Val Thr Asn Phe Leu Ala Gly Phe Glu Pro 225 230235 49 1066 DNA Rattus norvegicus F1-ATPase alpha subunit 49 gtggctgaccgccagatgtc tctgctgctc cgccgacccc cgggtcgaga ggcctatccc 60 ggtgatgtgttttacctaca ctctcgcctg ctggagagag cagccaagat gaacgattcc 120 tttggtggtggctctttgac tgccttacca gtcattgaaa cacaggctgg tgatgtgtcc 180 gcctacattccaacaaacgt tatttccatc accgatggac agatcttctt ggaaacagaa 240 ttgttctataaaggcatccg ccctgccatt aatgtgggct tgtctgtgtc ccgtgtcgga 300 tctgccgcccagaccagagc catgaagcag gtggcaggca ccatgaagct ggagttggcc 360 cagtaccgggaggtcgctgc ttttgcccag tttggttctg atctggatgc tgccactcag 420 cagctcttgagccgtggcgt gcgcctgacc gagctgctaa agcaaggaca gtactctccc 480 atggctattgaagaacaggt ggctgtcatc tatgcaggcg tccggggtta tcttgataaa 540 ctggagcccagtaagatcac aaagttcgag agtgctttct tgtctcatgt tgtgagccag 600 caccagagcctcttgggcaa tatcaggtct gatgggaaaa tctcagaaca gtcggatgca 660 aagctgaaggaaatcgtaac aaacttcttg gctgggtttg aaccttaaag ccctgccact 720 gtcaccagacactgctttgg ttttgtcatt tattgtggta aaatcagcac catttgtaaa 780 ggtttactcttgtactccct gatgtacaga aatcacatga ataaaagttc catattgagt 840 gctagtttgtgtgggacggg ctcagatgta tcctgggact tgtggaggtc agagagcagc 900 ctgtaggagtcagtgccctc ctttccctgg gaatttaact caggttatta tttaaagctc 960 gacttcaggggttggggatt tagctcagtg gtagagcgct tgcctaggaa gcgcaaggcc 1020 ctgggttcggtccccagctc cggaaaaaaa agaaccaacc cgaatt 1066 50 168 PRT Rattusnorvegicus F1F0 ATPase delta subunit 50 Met Leu Pro Ala Ala Leu Leu ArgHis Pro Gly Leu Arg Arg Leu Val 1 5 10 15 Leu Gln Ala Arg Thr Tyr AlaGln Ala Ala Ala Ser Pro Ala Pro Ala 20 25 30 Ala Gly Pro Gly Gln Met SerPhe Thr Phe Ala Ser Pro Thr Gln Val 35 40 45 Phe Phe Asp Gly Ala Asn ValArg Gln Val Asp Val Pro Thr Leu Thr 50 55 60 Gly Ala Phe Gly Ile Leu AlaSer His Val Pro Thr Leu Gln Val Leu 65 70 75 80 Arg Pro Gly Leu Val MetVal His Ala Glu Asp Gly Thr Thr Thr Lys 85 90 95 Tyr Phe Val Ser Ser GlySer Val Thr Val Asn Ala Asp Ser Ser Val 100 105 110 Gln Leu Leu Ala GluGlu Val Val Thr Leu Asp Met Leu Asp Leu Gly 115 120 125 Ala Ala Arg AlaAsn Leu Glu Lys Ala Gln Ser Glu Leu Ser Gly Ala 130 135 140 Ala Asp GluAla Ala Arg Ala Glu Ile Gln Ile Arg Ile Glu Ala Asn 145 150 155 160 GluAla Leu Val Lys Ala Leu Glu 165 51 811 DNA Rattus norvegicus F1F0 ATPasedelta subunit 51 ggaattccgc tgctgtcgct aagctaaagt cactgacgtt tccgccaccatgctgcccgc 60 cgcattgctt cgtcacccag gtctgcgccg tctggtgctc caggcgcgtacgtacgccca 120 ggccgccgcc tcacctgccc ccgccgctgg gcctggacag atgtccttcaccttcgcctc 180 cccgacgcag gtgttctttg atggtgccaa tgtccggcaa gtggatgtgcctacgctgac 240 tggagccttt ggcatcctgg catcccatgt ccccacacta caggtcctacggcctgggct 300 ggtaatggtt catgcggaag atggcaccac aactaagtat tttgtgagcagcggctccgt 360 cactgtgaat gcggactcct ctgtgcaatt actagctgaa gaagtcgtgacactggacat 420 gctggacctc ggggcagccc gggcaaacct ggagaaggcg cagtcagagctgtcaggggc 480 agcagatgag gcagcacggg ctgagatcca aatccggatc gaggccaatgaagccctggt 540 gaaggccctg gagtaggcgt actttgtctg tcacccacag ggtaacagaggcaggtcctg 600 gggggctgaa gtcgccacca gggggcagca gtgctccggt tactggcttaaagcttccct 660 ggtgctgcct gccaggtcat ggagggcttc cccaggcttc cagagtctgggatccccagg 720 atgcctctgg agagctggcc ttgattgccc ctcaaagcca cccggacagtcagctggccc 780 agcctatctg cattaaatac cacggaattc c 811 52 572 PRT Homosapiens Dihydropyrimidinase-related protein 52 Met Ser Tyr Gln Gly LysLys Asn Ile Pro Arg Ile Thr Ser Asp Arg 1 5 10 15 Leu Leu Ile Lys GlyGly Lys Ile Val Asn Asp Asp Gln Ser Phe Tyr 20 25 30 Ala Asp Ile Tyr MetGlu Asp Gly Leu Ile Lys Gln Ile Gly Glu Asn 35 40 45 Leu Ile Val Pro GlyGly Val Lys Thr Ile Glu Ala His Ser Arg Met 50 55 60 Val Ile Pro Gly GlyIle Asp Val His Thr Arg Phe Gln Met Pro Asp 65 70 75 80 Gln Gly Met ThrSer Ala Asp Asp Phe Phe Gln Gly Thr Lys Ala Ala 85 90 95 Leu Ala Gly GlyThr Thr Met Ile Ile Asp His Val Val Pro Glu Pro 100 105 110 Gly Thr SerLeu Leu Ala Ala Phe Asp Gln Trp Arg Glu Trp Ala Asp 115 120 125 Ser LysSer Cys Cys Asp Tyr Ser Leu His Val Asp Ile Ser Glu Trp 130 135 140 HisLys Gly Ile Gln Glu Glu Met Glu Ala Leu Val Lys Asp His Gly 145 150 155160 Val Asn Ser Phe Leu Val Tyr Met Ala Phe Lys Asp Arg Phe Gln Leu 165170 175 Thr Asp Cys Gln Ile Tyr Glu Val Leu Ser Val Ile Arg Asp Ile Gly180 185 190 Ala Ile Ala Gln Val His Ala Glu Asn Gly Asp Ile Ile Ala GluGlu 195 200 205 Gln Gln Arg Ile Leu Asp Leu Gly Ile Thr Gly Pro Glu GlyHis Val 210 215 220 Leu Ser Arg Pro Glu Glu Val Glu Ala Glu Ala Val AsnArg Ala Ile 225 230 235 240 Thr Ile Ala Asn Gln Thr Asn Cys Pro Leu TyrIle Thr Lys Val Met 245 250 255 Ser Lys Ser Ser Ala Glu Val Ile Ala GlnAla Arg Lys Lys Gly Thr 260 265 270 Val Val Tyr Gly Glu Pro Ile Thr AlaSer Leu Gly Thr Asp Gly Ser 275 280 285 His Tyr Trp Ser Lys Asn Trp AlaLys Ala Ala Ala Phe Val Thr Ser 290 295 300 Pro Pro Leu Ser Pro Asp ProThr Thr Pro Asp Phe Leu Asn Ser Leu 305 310 315 320 Leu Ser Cys Gly AspLeu Gln Val Thr Gly Ser Ala His Cys Thr Phe 325 330 335 Asn Thr Ala GlnLys Ala Val Gly Lys Asp Asn Phe Thr Leu Ile Pro 340 345 350 Glu Gly ThrAsn Gly Thr Glu Glu Arg Met Ser Val Ile Trp Asp Lys 355 360 365 Ala ValVal Thr Gly Lys Met Asp Glu Asn Gln Phe Val Ala Val Thr 370 375 380 SerThr Asn Ala Ala Lys Val Phe Asn Leu Tyr Pro Arg Lys Gly Arg 385 390 395400 Ile Ala Val Gly Ser Asp Ala Asp Leu Val Ile Trp Asp Pro Asp Ser 405410 415 Val Lys Thr Ile Ser Ala Lys Thr His Asn Ser Ser Leu Glu Tyr Asn420 425 430 Ile Phe Glu Gly Met Glu Cys Arg Gly Ser Pro Leu Val Val IleSer 435 440 445 Gln Gly Lys Ile Val Leu Glu Asp Gly Thr Leu His Val ThrGlu Gly 450 455 460 Ser Gly Arg Tyr Ile Pro Arg Lys Pro Phe Pro Asp PheVal Tyr Lys 465 470 475 480 Arg Ile Lys Ala Arg Ser Arg Leu Ala Glu LeuArg Gly Val Pro Arg 485 490 495 Gly Leu Tyr Asp Gly Pro Val Cys Glu ValSer Val Thr Pro Lys Thr 500 505 510 Val Thr Pro Ala Ser Ser Ala Lys ThrSer Pro Ala Lys Gln Gln Ala 515 520 525 Pro Pro Val Arg Asn Leu His GlnSer Gly Phe Ser Leu Ser Gly Ala 530 535 540 Gln Ile Asp Asp Asn Ile ProArg Arg Thr Thr Gln Arg Ile Val Ala 545 550 555 560 Pro Pro Gly Gly ArgAla Asn Ile Thr Ser Leu Gly 565 570 53 4459 DNA Homo sapiensDihydropyrimidinase-related protein 53 gtttctctct ctccttctct ctctctctctctctctcttt tttttccgcc ctagctgggg 60 ctgtgttgga ggagaggaag aaagagagacagaggattgc attcatccgt tacgttcttg 120 aaatttccta atagcaagac cagcgaagcggttgcaccct tttcaatctt gcaaaggaaa 180 aaaacaaaac aaaacaaaaa aaacccaagtccccttcccg gcagtttttg ccttaaagct 240 gccctcttga aattaatttt ttcccaggagagagatgtct tatcagggga agaaaaatat 300 tccacgcatc acgagcgatc gtcttctgatcaaaggaggt aaaattgtta atgatgacca 360 gtcgttctat gcagacatat acatggaagatgggttgatc aagcaaatag gagaaaatct 420 gattgtgcca ggaggagtga agaccatcgaggcccactcc cggatggtga tccccggagg 480 aattgacgtc cacactcgtt tccagatgcctgatcaggga atgacgtctg ctgatgattt 540 cttccaagga accaaggcgg ccctggctgggggaaccact atgatcattg accacgttgt 600 tcctgagcct gggacaagcc tgctcgctgcctttgaccag tggagggaat gggccgacag 660 caagtcctgc tgtgactact ctctgcatgtggacatcagc gagtggcata agggcatcca 720 ggaggagatg gaagcgcttg tgaaggatcacggggtaaat tccttcctcg tgtacatggc 780 tttcaaagat cgcttccagc taacggattgccagatttat gaagtactga gtgtgatccg 840 ggatattggc gccatagccc aagtccacgcagaaaatggc gacatcattg cagaggagca 900 gcagaggatc ctggatctgg gcatcacgggccccgaggga catgtgctga gccgacctga 960 ggaggtcgag gccgaagccg tgaatcgtgccatcaccatc gccaaccaga ccaactgccc 1020 gctgtatatc accaaggtga tgagcaaaagctctgctgag gtcatcgccc aggcacggaa 1080 gaagggaact gtggtgtatg gcgagcccatcactgccagc ttgggaacgg acggctccca 1140 ttactggagc aagaactggg ccaaggctgctgcctttgtc acctccccac ccttgagccc 1200 tgatccaacc actccagact ttctcaactccttgctgtcc tgtggagacc tccaggtcac 1260 gggcagtgcc cattgcacgt ttaacactgcccagaaggct gtaggaaagg acaacttcac 1320 cctgattccg gagggcacca atggcactgaggagcggatg tccgtcatct gggacaaggc 1380 tgtggtcact gggaagatgg atgagaaccagtttgtggct gtgaccagca ccaatgcagc 1440 caaagtcttc aacctttacc cccggaaaggccgcattgct gtgggatccg atgccgacct 1500 ggtcatctgg gaccccgaca gcgttaaaaccatctctgcc aagacacaca acagctctct 1560 cgagtacaac atctttgaag gcatggagtgccgcggctcc ccactggtgg tcatcagcca 1620 ggggaagatt gtcctggagg acggcaccctgcatgtcacc gaaggctctg gacgctacat 1680 tccccggaag cccttccctg attttgtttacaagcgtatc aaggcaagga gcaggctggc 1740 tgagctgaga ggggttcctc gtggcctgtatgacggacct gtgtgtgaag tgtctgtgac 1800 gcccaagaca gtcactccag cctcctcggccaagacgtct cctgccaagc agcaggcccc 1860 acctgtccgg aacctgcacc agtctggattcagtttgtct ggtgctcaga ttgatgacaa 1920 cattccccgc cgcaccaccc agcgtatcgtggcgcccccc ggtggccgtg ccaacatcac 1980 cagcctgggc tagagctcct gggctgtgcgtccactgggg actggggatg ggacacctga 2040 ggacattctg agacttcttt cttccttccttttttttttt ttgttttttt ttttaagagc 2100 ctgtgatagt tactgtggag cagccagttcatggggtccc ccttggggcc ccacaccccg 2160 tctctcacca agagttactg attttgctcatccacttccc tacacatcta tgggtatcac 2220 acccaagact acccaccaag ctcatacagggaaccacacc caacacttag acatgcgaac 2280 aagcagcccc cagcgagggt ctccttcgccttcaacctcc tagtgtctgt tagcatcttc 2340 cttttcatgg ggggagggaa gataaagtgaattgcccaga gctgcctttt tcttttcttt 2400 ttaaaaattt taagaagttt tccttgtggggctggggagg ggccggggtc agggagagtc 2460 tttttttttt tttttttaaa tactaaattggaacatttaa ttccatatta atacaagggg 2520 tttgaactgg acatcctaat gatgcaattacgtcatcacc cagctgattc cgggtggttg 2580 gcaaactcat cgtgtctgtc ctgagaggctccacaatgcc cacccgcatc gccattctgt 2640 agtcttcagg gtcagctgtt gataaaggggcaggcttgcg ttattggcct agattttgct 2700 gcagattaaa tcctttgagg attctcttctcttttaccat ttttctgcgt gctctcactc 2760 tctctttctc tctctagctt tttaattcatgaatattttc gtgtctgtct ctctctctct 2820 ctgtgtttcc tccagccctt gtctcggagacggtgttttc ctcccttgcc ccattatctt 2880 ttcacctccc aggtctacca tttcatggtggtcgttgggt ccgcctaaag gatttgagcg 2940 tttgccattg caagcatagt gctgtgtcatcctggtccat gtaggactgg tgctaaccac 3000 ctgccatcat gaggatgtgt gctagagtgtgggaccctgg ccaagtgcag gaatgggcca 3060 tgccgtctca cccacagtat cacacgtggaaccgcagaca gggcccagaa gctttagagg 3120 tatgaggctg cagaaccgga gagattttcctctgtgcagt gctctctggc taaagtcacg 3180 gtcaaaccta aacaccgagc ctcattaacccaagtgaacc aaccaaagtc accagttcag 3240 aagtgctaag ctaataggag tctgacccgagggcctgctg cttcctggtt aagtatcttt 3300 tgagattcta gaacacatgg gagctttttattttcgggga aaaaccgtat ttttttcttg 3360 tccaattatt tctaaagaca cactacatagaaagaggccc tataaactca aaaagtcatt 3420 gggaaactta aagtctattc tactttgcaagaggagaaat gtgttttatg aacgatagat 3480 cacatcagaa ctcctgtggg gaggaaaccttataaattaa acacatggcc cccttagaga 3540 ccacaggtga tgtctgtctc catccttccctctccttttc tgtcaccttt ccccctagct 3600 ggctcctttg gacctacccc tgtccttgctgacttgtgtt gcattgtatt ccaaacgtgt 3660 ttacaggttc tcttaagcaa tgttgtatttgcaggctttt ctgaatacca aatctgcttt 3720 ttgtaaagcg taaaaacatc acaaagtaggtcattccatc accacccttg tctctctaca 3780 cattttgcct ttggggatct ggttggggttttgggttttt tgttgttgtt gtttatttgt 3840 tattttaaag gtaaattgca cttttaaaaaaataattggt tgacttaata tatttgcttt 3900 ttttctcacc tgcacttaga ggaaatttgaacaagttgga aaaaaacaat ttttgtttca 3960 attctaagaa acacttgcag ctctagtattcacttgagtc ttcctgtttt tcctgtaccg 4020 ggtcatggta atttttggtt gttttggttgttttcttaaa aaacaagtta aaacctgacg 4080 atttctgcag gctgtgtaag catgtttacctgttggcttg ctttgtgtgt ctgttaaatg 4140 aatgtcatat gtaaatgcta aaataaatcgacagtgtctc agaactgaat aactgcagtg 4200 acttgatgct ctaaaacagt gtaggatttaagaatagatg gtttttaatc ctggaaattg 4260 tgattgtgac ccatgagtgg aggaactttcagttctaaag ctgataaagt gtgtagccag 4320 aagagtactt ttttttttgt aaccactgtcttgatggcaa aataattatg gtaaaaaaca 4380 agtctcgtgt ttattattcc ttaagaactctgtgttatat taccatggaa cgcctaataa 4440 agcaaaatgt ggttgtttc 4459 54 1410DNA Rattus norvegicus Myelin basic protein S 54 ttcggcttcc gaaggcctgatgtgatggca tcacagaaga gaccctcaca gcgacacgga 60 tccaagtact tggccacagcaagtaccatg gaccatgccc ggcatggctt cctcccaagg 120 cacagagaca cgggcatccttgactccatc gggcgcttct ttagcggtga caggggtgcg 180 cccaagcggg gctctggcaaggactcacac acaagaacta cccactacgg ctccctgccc 240 cagaagtcgc agaggacccaagatgaaaac ccagtagtcc acttcttcaa gaacattgtg 300 acacctcgta caccccctccatcccaagga aaggggagag gcctgtccct cagcagattt 360 agctggggag gaagagacagccgctctgga tctcccatag caagacgctg agagcctccc 420 tgctcagcct tcccgaatcctgccctcggc ttcttaatat aactgcctta aacgtttaat 480 tctacttgca ccaaatagctagttagagca gaccctctct taatcccgtg gggctgtgaa 540 cgcggcgggc cagcccacggcaccctgact ggctaaaact gtttgtccct ttttatttga 600 agattgagtt tcctcggggtcttctctgcc ccgacttgct ccccgtgtac cttggtcgac 660 tccggaggtt caggtgcacggacacccttt caagttcacc cctactccat cctcagactt 720 tcttttcacg gcgaggcgcacccctccagc ttccgtgggc actgcggata gacaggcaca 780 ccgccaagga gccagagagcatggcgcagg ggactatgtg gtccaggctt cctttgtttt 840 ctttccccta aagagctttgtttttcctga caggatcaga cagtcttgga gtggcttaca 900 caacgggggc ttgtggtatgtgagcacagg tcgggcagct gtgagagtcc agagtggggt 960 ggccctgggg acgcttccaggccagcggtt ccctgcaccc caccagctga tttcgagcgt 1020 ggcagaggga aggaaaggggcgagcgggct gggcaatgga cccgacagga aacggggact 1080 taggggaaca cgctggagatgccatgtgtg gctgccgaag gtcaccatct ctcctcagtg 1140 ctccccagag caggtgcttttaagaaccct gtttcctctc agagcctagg gagagtccaa 1200 ggacatggcg catcaggaagtgggactgca ggagttctct ggtggcctcg tgctgtccct 1260 ctggccactt ctcactttagggtggtcacg gcagctcgcc atggcagtgc cattggtgca 1320 cactaacctc atggaaaagtaaccattccc tgcctcttag aaagaactca ttcttagttt 1380 taggagggtt cctgtcgctgaatcaagtcg 1410 55 698 PRT Rattus norvegicus Transferrin 55 Met Arg PheAla Val Gly Ala Leu Leu Ala Cys Ala Ala Leu Gly Leu 1 5 10 15 Cys LeuAla Val Pro Asp Lys Thr Val Lys Trp Cys Ala Val Ser Glu 20 25 30 His GluAsn Thr Lys Cys Ile Ser Phe Arg Asp His Met Lys Thr Val 35 40 45 Leu ProAla Asp Gly Pro Arg Leu Ala Cys Val Lys Lys Thr Ser Tyr 50 55 60 Gln AspCys Ile Lys Ala Ile Ser Gly Gly Glu Ala Asp Ala Ile Thr 65 70 75 80 LeuAsp Gly Gly Trp Val Tyr Asp Ala Gly Leu Thr Pro Asn Asn Leu 85 90 95 LysPro Val Ala Ala Glu Phe Tyr Gly Ser Leu Glu His Pro Gln Thr 100 105 110His Tyr Leu Ala Val Ala Val Val Lys Lys Gly Thr Asp Phe Gln Leu 115 120125 Asn Gln Leu Gln Gly Lys Lys Ser Cys His Thr Gly Leu Gly Arg Ser 130135 140 Ala Gly Trp Ile Ile Pro Ile Gly Leu Leu Phe Cys Asn Leu Pro Glu145 150 155 160 Pro Arg Lys Pro Leu Glu Lys Ala Val Ala Ser Phe Phe SerGly Ser 165 170 175 Cys Val Pro Cys Ala Asp Pro Val Ala Phe Pro Gln LeuCys Gln Leu 180 185 190 Cys Pro Gly Cys Gly Cys Ser Pro Thr Gln Pro PhePhe Gly Tyr Val 195 200 205 Gly Ala Phe Lys Cys Leu Arg Asp Gly Gly GlyAsp Val Ala Phe Val 210 215 220 Lys His Thr Thr Ile Phe Glu Val Leu ProGln Lys Ala Asp Arg Asp 225 230 235 240 Gln Tyr Glu Leu Leu Cys Leu AspAsn Thr Arg Lys Pro Val Asp Gln 245 250 255 Tyr Glu Asp Cys Tyr Leu AlaArg Ile Pro Ser His Ala Val Val Ala 260 265 270 Arg Asn Gly Asp Gly LysGlu Asp Leu Ile Trp Glu Ile Leu Lys Val 275 280 285 Ala Gln Glu His PheGly Lys Gly Lys Ser Lys Asp Phe Gln Leu Phe 290 295 300 Gly Ser Pro LeuGly Lys Asp Leu Leu Phe Lys Asp Ser Ala Phe Gly 305 310 315 320 Cys TyrGly Val Pro Pro Arg Met Asp Tyr Arg Leu Tyr Leu Gly His 325 330 335 SerTyr Val Thr Ala Ile Arg Asn Gln Arg Glu Gly Val Cys Pro Glu 340 345 350Ala Ser Ile Asp Ser Ala Pro Val Lys Trp Cys Ala Leu Ser His Gln 355 360365 Glu Arg Ala Lys Cys Asp Glu Trp Ser Val Thr Ser Asn Gly Gln Ile 370375 380 Glu Cys Glu Ser Ala Glu Ser Thr Glu Asp Cys Ile Asp Lys Ile Val385 390 395 400 Asn Gly Glu Ala Asp Ala Met Ser Leu Asp Gly Gly His AlaTyr Ile 405 410 415 Ala Gly Gln Cys Gly Leu Val Pro Val Met Ala Glu AsnTyr Asp Ile 420 425 430 Ser Ser Cys Thr Asn Pro Gln Ser Asp Val Phe ProLys Gly Tyr Tyr 435 440 445 Ala Val Ala Val Val Lys Ala Ser Asp Ser SerIle Asn Trp Asn Asn 450 455 460 Leu Lys Gly Lys Lys Ser Cys His Thr GlyVal Asp Arg Thr Ala Gly 465 470 475 480 Trp Asn Ile Pro Met Gly Leu LeuPhe Ser Arg Ile Asn His Cys Lys 485 490 495 Phe Asp Glu Phe Phe Ser GlnGly Cys Ala Pro Gly Tyr Lys Lys Asn 500 505 510 Ser Thr Leu Cys Asp LeuCys Ile Gly Pro Ala Lys Cys Ala Pro Asn 515 520 525 Asn Arg Glu Gly TyrAsn Gly Tyr Thr Gly Ala Phe Gln Cys Leu Val 530 535 540 Glu Lys Gly AspVal Ala Phe Val Lys His Gln Thr Val Leu Glu Asn 545 550 555 560 Thr AsnGly Lys Asn Thr Ala Ala Trp Ala Lys Asp Leu Lys Gln Glu 565 570 575 AspPhe Gln Leu Leu Cys Pro Asp Gly Thr Lys Lys Pro Val Thr Glu 580 585 590Phe Ala Thr Cys His Leu Ala Gln Ala Pro Asn His Val Val Val Ser 595 600605 Arg Lys Glu Lys Ala Ala Arg Val Ser Thr Val Leu Thr Ala Gln Lys 610615 620 Asp Leu Phe Trp Lys Gly Asp Lys Asp Cys Thr Gly Asn Phe Cys Leu625 630 635 640 Phe Arg Ser Ser Thr Lys Asp Leu Leu Phe Arg Asp Asp ThrLys Cys 645 650 655 Leu Thr Lys Leu Pro Glu Gly Thr Thr Tyr Glu Glu TyrLeu Gly Ala 660 665 670 Glu Tyr Leu Gln Ala Val Gly Asn Ile Arg Lys CysSer Thr Ser Arg 675 680 685 Leu Leu Glu Ala Cys Thr Phe His Lys Ser 690695 56 2293 DNA Rattus norvegicus Transferrin 56 cgtccgctcc tcgccacacacaccgagagg atgaggttcg ctgtgggtgc cctgctggct 60 tgtgccgccc tgggactgtgtctggctgtc cctgacaaaa cggtcaaatg gtgcgcagtg 120 tctgagcatg agaacaccaagtgtatcagt ttccgtgacc acatgaaaac cgtccttcca 180 gctgatggcc cccggcttgcctgtgtgaag aaaacctcct atcaagattg catcaaggcc 240 atttctggag gtgaagctgatgccattacc ttggatgggg gttgggtgta cgatgcaggc 300 ctgactccca acaacctgaagcctgtggca gcagagtttt atggatcact tgaacatcca 360 cagacccact acttggctgtggccgtggtg aagaagggaa cagacttcca gctgaaccag 420 ctccagggca agaagtcctgccacactggc ctgggcaggt ctgcaggctg gattatcccc 480 attggcttac ttttctgtaacttgccagag ccccgcaagc ctcttgagaa agctgtggcc 540 agtttcttct cgggcagttgtgtcccctgt gcagatccag tggccttccc ccagctgtgt 600 caactgtgtc caggctgtggctgctccccg actcaaccgt tctttggcta cgtaggcgcc 660 ttcaagtgtc tgagagatggaggtggagat gtggcctttg tcaagcatac aaccatattt 720 gaggtcttgc cacagaaggctgacagggat caatatgagc tgctctgcct tgacaatacc 780 cgcaagccag tggatcagtatgaggactgc tacctagccc ggatcccttc tcatgctgtt 840 gtggctcgaa atggagatggcaaagaggac ttgatctggg agatcctcaa agtggctcag 900 gaacactttg gcaaaggcaaatcaaaagac ttccaactgt tcggctctcc tcttgggaaa 960 gacctgctgt ttaaggattctgcctttggc tgttacgggg tgcccccaag gatggactac 1020 aggctgtacc tcggccacagctatgtcact gccattcgaa atcagcggga aggcgtgtgc 1080 ccggaggcct ccatcgacagcgcgccagtg aaatggtgtg cactgagtca ccaagagaga 1140 gccaagtgtg atgagtggagcgtcacgagc aatgggcaga tagagtgtga gtcagcagag 1200 agcactgagg actgcattgacaagattgtg aatggagaag cagatgccat gagcttggat 1260 ggaggtcatg cctacatagcaggccagtgt ggactagtgc ccgtcatggc agagaactat 1320 gatatctctt cgtgtacaaacccacaatca gatgtctttc ctaaagggta ttatgccgtg 1380 gctgtggtga aggcatcagactccagcatc aactggaaca acctgaaagg caagaagtcc 1440 tgccatactg gagtagacagaaccgccggc tggaacatcc ctatgggcct gctgttcagc 1500 aggatcaacc actgcaagttcgatgaattt ttcagtcaag gctgtgctcc tggctataag 1560 aagaattcca ccctctgtgacctgtgtatt ggcccagcaa aatgtgctcc gaacaacaga 1620 gagggatata atggttatacaggggctttc cagtgcctcg ttgagaaggg agacgtagcc 1680 tttgtgaagc accagactgtcctggaaaac acgaacggaa agaacactgc tgcatgggct 1740 aaggatctga agcaggaagacttccagctg ctgtgccctg atggtaccaa gaagcctgta 1800 accgagttcg ccacctgccacctggcccaa gctccaaacc atgttgtggt ctcacgaaaa 1860 gagaaggcag cccgggttagcactgtgctg actgcccaga aggatttatt ttggaaaggt 1920 gacaaggact gcactggcaatttctgtttg ttccggtctt ccaccaagga ccttctgttc 1980 agagatgaca ccaagtgtttgactaaactt ccagaaggta ccacatatga agagtactta 2040 ggagcagagt acttgcaagctgttggaaac ataaggaagt gttcaacctc acgactccta 2100 gaagcctgca ctttccacaaaagttaaaat ccaagaggtg ggtgccactg tggtggagga 2160 ggatgccccc gtgatccatgggcttctcct ggcctccata gccctgagcg gctggggcta 2220 actgtgtccg tcttactgcttggtgttgtt accacataca cagagcacaa aataaaaaat 2280 gactgttgac ttt 2293 57542 PRT Rattus norvegicus NF-L 57 Met Ser Ser Phe Ser Tyr Glu Pro TyrPhe Ser Thr Ser Tyr Lys Arg 1 5 10 15 Arg Tyr Val Glu Thr Pro Arg ValHis Ile Ser Ser Val Arg Ser Gly 20 25 30 Tyr Ser Thr Ala Arg Ser Ala TyrSer Ser Tyr Ser Ala Pro Val Ser 35 40 45 Ser Ser Leu Ser Val Arg Arg SerTyr Ser Ser Ser Ser Gly Ser Leu 50 55 60 Met Pro Ser Leu Glu Asn Leu AspLeu Ser Gln Val Ala Ala Ile Ser 65 70 75 80 Asn Asp Leu Lys Ser Ile ArgThr Gln Glu Lys Ala Gln Leu Gln Asp 85 90 95 Leu Asn Asp Arg Phe Ala SerPhe Ile Glu Arg Val His Glu Leu Glu 100 105 110 Gln Gln Asn Lys Val LeuGlu Ala Glu Leu Leu Val Leu Arg Gln Lys 115 120 125 His Ser Glu Pro SerArg Phe Arg Ala Leu Tyr Glu Gln Glu Ile Arg 130 135 140 Asp Leu Arg LeuAla Ala Glu Asp Ala Thr Asn Glu Lys Gln Ala Leu 145 150 155 160 Gln GlyGlu Arg Glu Gly Leu Glu Glu Thr Leu Arg Asn Leu Gln Ala 165 170 175 ArgTyr Glu Glu Glu Val Leu Ser Arg Glu Asp Ala Glu Gly Arg Leu 180 185 190Met Glu Ala Arg Lys Gly Ala Asp Glu Ala Ala Leu Ala Arg Ala Glu 195 200205 Leu Glu Lys Arg Ile Asp Ser Leu Met Asp Glu Ile Ala Phe Leu Lys 210215 220 Lys Val His Glu Glu Glu Ile Ala Glu Leu Gln Ala Gln Ile Gln Tyr225 230 235 240 Ala Gln Ile Ser Val Glu Met Asp Val Ser Ser Lys Pro AspLeu Ser 245 250 255 Ala Ala Leu Lys Asp Ile Arg Ala Gln Tyr Glu Lys LeuAla Ala Lys 260 265 270 Asn Met Gln Asn Ala Glu Glu Trp Phe Lys Ser ArgPhe Thr Val Leu 275 280 285 Thr Glu Ser Ala Ala Lys Asn Thr Asp Ala ValArg Ala Ala Lys Asp 290 295 300 Glu Val Ser Glu Ser Arg Arg Leu Leu LysAla Lys Thr Leu Glu Ile 305 310 315 320 Glu Ala Cys Arg Gly Met Asn GluAla Leu Glu Lys Gln Leu Gln Glu 325 330 335 Leu Glu Asp Lys Gln Asn AlaAsp Ile Ser Ala Met Gln Asp Thr Ile 340 345 350 Asn Lys Leu Glu Asn GluLeu Arg Ser Thr Lys Ser Glu Met Ala Arg 355 360 365 Tyr Leu Lys Glu TyrGln Asp Leu Leu Asn Val Lys Met Ala Leu Asp 370 375 380 Ile Glu Ile AlaAla Tyr Arg Lys Leu Leu Glu Gly Glu Glu Thr Arg 385 390 395 400 Leu SerPhe Thr Ser Val Gly Ser Ile Thr Ser Gly Tyr Ser Gln Ser 405 410 415 SerGln Val Phe Gly Arg Ser Ala Tyr Ser Gly Leu Gln Ser Ser Ser 420 425 430Tyr Leu Met Ser Ala Arg Ala Phe Pro Ala Tyr Tyr Thr Ser His Val 435 440445 Gln Glu Glu Gln Ser Glu Val Glu Glu Thr Ile Glu Ala Thr Lys Ala 450455 460 Glu Glu Ala Lys Asp Glu Pro Pro Ser Glu Gly Glu Ala Glu Glu Glu465 470 475 480 Glu Lys Glu Lys Glu Glu Gly Glu Glu Glu Glu Gly Ala GluGlu Glu 485 490 495 Glu Ala Ala Lys Asp Glu Ser Glu Asp Ala Lys Glu GluGlu Gly Gly 500 505 510 Glu Gly Glu Glu Glu Asp Thr Lys Glu Ser Glu GluGlu Glu Lys Lys 515 520 525 Glu Glu Ser Ala Gly Glu Glu Gln Ala Ala LysLys Lys Asp 530 535 540 58 2032 DNA Rattus norvegicus NF-L 58 gaattcccccggttcttctc tctaggtccc ctatctccgc cccgggcctg aggggcgcac 60 cgaccgccaccatgagttcg ttcagctacg agccgtactt ttcgacctcc tacaagcggc 120 gctacgtggagacgccccgg gtgcacatct ccagcgtgcg cagcggctac agcacggcgc 180 gctctgcgtactccagctac tccgcgcccg tctcctcctc tctgtcggtg cgccgcagct 240 actcatccagctccggctct ttgatgccca gcctggagaa cctcgatctg agccaggtag 300 ccgccatcagcaacgacctc aagtctatcc gcacacagga gaaggcacag ctgcaggacc 360 tcaacgatcgcttcgccagc ttcatcgagc gcgtgcacga gctggagcag cagaacaagg 420 tcctggaagccgagctgttg gtgctgcgcc agaagcactc agagccttcc cgcttccgcg 480 ccctgtatgagcaggagatc cgtgatctgc gactggcggc cgaagacgcc actaacgaga 540 agcaggcgctgcagggcgag cgcgaggggc tggaggagac tctgcgcaac ctgcaggctc 600 gctacgaggaggaggtgctg agccgcgagg acgccgaggg ccggctgatg gaagcccgca 660 aaggcgcggatgaggctgcg ctcgcccgcg ccgagctgga gaagcgcatc gacagcctga 720 tggacgagatagccttcctg aaaaaggtgc acgaggaaga gatcgccgag ctgcaggctc 780 agatccagtatgctcagatc tccgtggaga tggacgtgtc ctccaagccc gacctctccg 840 ccgctctcaaggacatccgc gctcagtacg agaagctggc cgccaagaat atgcagaatg 900 ccgaagagtggttcaagagc cgcttcacgg tgctaaccga gagcgccgcc aagaacaccg 960 acgcagtgcgcgctgccaag gacgaggtgt cggaaagccg ccgcctactt aaggctaaga 1020 ccctggagatcgaagcctgc cggggtatga acgaagctct agagaagcag ctgcaggagc 1080 tggaggacaagcagaatgca gacatcagcg ccatgcagga cacaatcaac aaactggaga 1140 atgagctgcgaagcacgaag agcgagatgg ccaggtacct gaaggagtac caggacctcc 1200 tcaatgtcaagatggcattg gacattgaga ttgcagctta caggaaactc ttggaaggcg 1260 aagaaaccaggctcagtttc accagcgtgg gtagcataac cagcggctac tctcagagct 1320 cgcaggtctttggccgttct gcttacagtg gcttgcagag cagctcctac ttgatgtctg 1380 ctcgagcattcccagcctac tataccagcc acgtccagga agagcagtca gaggtggagg 1440 agaccattgaggctacgaaa gctgaggagg ccaaggatga gcccccctct gaaggagaag 1500 cagaagaggaggagaaggag aaagaggagg gggaggaaga ggaaggtgct gaggaggaag 1560 aagctgccaaggatgagtct gaagatgcca aagaagaaga aggtggtgag ggtgaagagg 1620 aagacaccaaagagtcagaa gaggaagaga agaaagagga gagtgctggg gaggagcaag 1680 ctgctaagaagaaagattga gccccattcc caactatccc aggaaaaaag tctccccaaa 1740 tcaggtcaacctcatcacca aaccaaccag ttgagttcca gatcctatac agattaagaa 1800 gtcaatatatgtataattct gagatgactt aggttggaca ttcaatgttg tgctatgact 1860 ttcctccttatgcagagtat ctgtttgctt gcagagtggc tttctggctt gctgccaacc 1920 tgtgcatggtccatgcttat gagttcagga tctacggcaa tgtgaatcac acagatgttt 1980 acaataataataataaaaaa accacacata cacaacatga ataaatgaat tc 2032 59 626 PRT Rattusnorvegicus MAG 59 Met Ile Phe Leu Thr Thr Leu Pro Leu Phe Trp Ile MetIle Ser Ala 1 5 10 15 Ser Arg Gly Gly His Trp Gly Ala Trp Met Pro SerSer Ile Ser Ala 20 25 30 Phe Glu Gly Thr Cys Val Ser Ile Pro Cys Arg PheAsp Phe Pro Asp 35 40 45 Glu Leu Arg Pro Ala Val Val His Gly Val Trp TyrPhe Asn Ser Pro 50 55 60 Tyr Pro Lys Asn Tyr Pro Pro Val Val Phe Lys SerArg Thr Gln Val 65 70 75 80 Val His Glu Ser Phe Gln Gly Arg Ser Arg LeuLeu Gly Asp Leu Gly 85 90 95 Leu Arg Asn Cys Thr Leu Leu Leu Ser Thr LeuSer Pro Glu Leu Gly 100 105 110 Gly Lys Tyr Tyr Phe Arg Gly Asp Leu GlyGly Tyr Asn Gln Tyr Thr 115 120 125 Phe Ser Glu His Ser Val Leu Asp IleIle Asn Thr Pro Asn Ile Val 130 135 140 Val Pro Pro Glu Val Val Ala GlyThr Glu Val Glu Val Ser Cys Met 145 150 155 160 Val Pro Asp Asn Cys ProGlu Leu Arg Pro Glu Leu Ser Trp Leu Gly 165 170 175 His Glu Gly Leu GlyGlu Pro Thr Val Leu Gly Arg Leu Arg Glu Asp 180 185 190 Glu Gly Thr TrpVal Gln Val Ser Leu Leu His Phe Val Pro Thr Arg 195 200 205 Glu Ala AsnGly His Arg Leu Gly Cys Gln Ala Ala Phe Pro Asn Thr 210 215 220 Thr LeuGln Phe Glu Gly Tyr Ala Ser Leu Asp Val Lys Tyr Pro Pro 225 230 235 240Val Ile Val Glu Met Asn Ser Ser Val Glu Ala Ile Glu Gly Ser His 245 250255 Val Ser Leu Leu Cys Gly Ala Asp Ser Asn Pro Pro Pro Leu Leu Thr 260265 270 Trp Met Arg Asp Gly Met Val Leu Arg Glu Ala Val Ala Glu Ser Leu275 280 285 Tyr Leu Asp Leu Glu Glu Val Thr Pro Ala Glu Asp Gly Ile TyrAla 290 295 300 Cys Leu Ala Glu Asn Ala Tyr Gly Gln Asp Asn Arg Thr ValGlu Leu 305 310 315 320 Ser Val Met Tyr Ala Pro Trp Lys Pro Thr Val AsnGly Thr Val Val 325 330 335 Ala Val Glu Gly Glu Thr Val Ser Ile Leu CysSer Thr Gln Ser Asn 340 345 350 Pro Asp Pro Ile Leu Thr Ile Phe Lys GluLys Gln Ile Leu Ala Thr 355 360 365 Val Ile Tyr Glu Ser Gln Leu Gln LeuGlu Leu Pro Ala Val Thr Pro 370 375 380 Glu Asp Asp Gly Glu Tyr Trp CysVal Ala Glu Asn Gln Tyr Gly Gln 385 390 395 400 Arg Ala Thr Ala Phe AsnLeu Ser Val Glu Phe Ala Pro Ile Ile Leu 405 410 415 Leu Glu Ser His CysAla Ala Ala Arg Asp Thr Val Gln Cys Leu Cys 420 425 430 Val Val Lys SerAsn Pro Glu Pro Ser Val Ala Phe Glu Leu Pro Ser 435 440 445 Arg Asn ValThr Val Asn Glu Thr Glu Arg Glu Phe Val Tyr Ser Glu 450 455 460 Arg SerGly Leu Leu Leu Thr Ser Ile Leu Thr Leu Arg Gly Gln Ala 465 470 475 480Gln Ala Pro Pro Arg Val Ile Cys Thr Ser Arg Asn Leu Tyr Gly Thr 485 490495 Gln Ser Leu Glu Leu Pro Phe Gln Gly Ala His Arg Leu Met Trp Ala 500505 510 Lys Ile Gly Pro Val Gly Ala Val Val Ala Phe Ala Ile Leu Ile Ala515 520 525 Ile Val Cys Tyr Ile Thr Gln Thr Arg Arg Lys Lys Asn Val ThrGlu 530 535 540 Ser Pro Ser Phe Ser Ala Gly Asp Asn Pro His Val Leu TyrSer Pro 545 550 555 560 Glu Phe Arg Ile Ser Gly Ala Pro Asp Lys Tyr GluSer Glu Lys Arg 565 570 575 Leu Gly Ser Glu Arg Arg Leu Leu Gly Leu ArgGly Glu Pro Pro Glu 580 585 590 Leu Asp Leu Ser Tyr Ser His Ser Asp LeuGly Lys Arg Pro Thr Lys 595 600 605 Asp Ser Tyr Thr Leu Thr Glu Glu LeuAla Glu Tyr Ala Glu Ile Arg 610 615 620 Val Lys 625 60 2475 DNA Rattusnorvegicus MAG 60 cagaagccag accatccaac cttctgtatc agtgctcctc gtcgcctcactgtacttcac 60 ggaagagact tggttgactg gccacttgga gcggaatcag gagacattcccaactcaggg 120 agactgaggt gagggcccta gctcgcccac ttgctggaca agatgatattccttaccacc 180 ctgcctctgt tttggataat gatttcagct tctcgagggg ggcactggggtgcctggatg 240 ccctcgtcca tctcagcctt cgagggcacg tgtgtctcca tcccctgccgtttcgacttc 300 ccggatgagc tcagaccggc tgtggtacat ggcgtctggt atttcaacagtccctacccc 360 aagaactacc cgccagtggt cttcaagtcc cgcacacaag tggtccacgagagcttccag 420 ggccgtagcc gcctgttggg agacctgggc ctacgaaact gcaccctgcttctcagcacg 480 ctgagccctg agctgggagg gaaatactat ttccgaggtg acctgggcggctacaaccag 540 tacaccttct cggagcacag cgtcctggac atcatcaaca cccccaacatcgtggtgccc 600 ccagaagtgg tggcaggaac ggaagtagag gtcagctgca tggtgccggacaactgccca 660 gagctgcgcc ctgagctgag ctggctgggc cacgaggggc taggggagcccactgttctg 720 ggtcggctgc gggaggatga aggcacctgg gtgcaggtgt cactgctacacttcgtgcct 780 actagagagg ccaacggcca ccgtctgggc tgtcaggctg ccttccccaacaccaccttg 840 cagttcgagg gttacgccag tctggacgtc aagtaccccc cggtgattgtggagatgaat 900 tcctctgtgg aggccattga gggctcccat gtcagcctgc tctgtggggctgacagcaac 960 ccgccaccgc tgctgacttg gatgcgggat gggatggtgt tgagggaggcagttgctgag 1020 agcctgtacc tggatctgga ggaggtgacc ccagcagagg acggcatctatgcttgcctg 1080 gcagagaatg cctatggcca ggacaaccgc acggtggagc tgagcgtcatgtatgcacct 1140 tggaagccca cagtgaatgg gacggtggtg gcggtagagg gggagacagtctccatcctg 1200 tgttccacac agagcaaccc ggaccctatt ctcaccatct tcaaggagaagcagatcctg 1260 gccacggtca tctatgagag tcagctgcag ctggaactcc ctgcagtgacgcccgaggac 1320 gatggggagt actggtgtgt agctgagaac cagtatggcc agagagccaccgccttcaac 1380 ctgtctgtgg agtttgctcc cataatcctt ctggaatcgc actgtgcagcggccagagac 1440 accgtgcagt gcctgtgtgt ggtaaaatcc aacccggaac cctccgtggcctttgagctg 1500 ccttcccgca acgtgactgt gaacgagaca gagagggagt ttgtgtactcagagcgcagc 1560 ggcctcctgc tcaccagcat cctcacgctc cggggtcagg cccaagccccaccccgcgtc 1620 atttgtacct ccaggaacct ctacggcacc cagagcctcg agctgcctttccagggagca 1680 caccgactga tgtgggccaa aatcggccct gtgggtgctg tggtcgcctttgccatcctg 1740 attgccattg tctgctacat cacccagaca agaagaaaaa agaacgtcacagagagcccc 1800 agcttctcag cgggagacaa ccctcatgtc ctgtacagcc ccgaattccgaatctctgga 1860 gcacctgata agtatgagag tgagaagcgc ctggggtccg agaggaggctgctgggcctt 1920 aggggggaac ccccagaact ggacctcagt tattcccact cagacctggggaaacgaccc 1980 accaaggaca gctacaccct gacagaggag ctggctgagt acgcagaaatccgagtcaag 2040 tgaggaagct gggggctggc cctgtggctc accccccatc aggaccctcgcttggccccc 2100 actggccgtg ggctcccttt ctcttgagag tggtaggggt gggggcgggaaggggcgggg 2160 caggaaacag tgaggtctta ggggcccggc ctcccctcct tcccggctgctcctctctgc 2220 caacatcctg cacctatgtt acagctccct ctcccctcct tttaacctcagctgttgaga 2280 ggggtgctct gtctgtccat gttatttatt gttatcctgg tctcctgtccccttacccgg 2340 ccccaggacc tgtacaaaag ggacatgaaa taaatgtcct aatgacaagtgccagtctag 2400 acccatcctt tggaggaaag gggcatatta gtaatacttt tctcgttgctgtaacaaaat 2460 actggacaaa aacac 2475 61 845 PRT Rattus norvegicus NF-Mprotein 61 Met Ser Tyr Thr Leu Asp Ser Leu Gly Asn Pro Ser Ala Tyr ArgArg 1 5 10 15 Val Pro Thr Glu Thr Arg Ser Ser Phe Ser Arg Val Ser GlySer Pro 20 25 30 Ser Ser Gly Phe Arg Ser Gln Ser Trp Ser Arg Gly Ser ProSer Thr 35 40 45 Val Ser Ser Ser Tyr Lys Arg Ser Ala Leu Ala Pro Arg LeuAla Tyr 50 55 60 Ser Ser Ala Met Leu Ser Ser Ala Glu Ser Ser Leu Asp PheSer Gln 65 70 75 80 Ser Ser Ser Leu Leu Asn Gly Gly Ser Gly Gly Asp TyrLys Leu Ser 85 90 95 Arg Ser Asn Glu Lys Glu Gln Leu Gln Gly Leu Asn AspArg Phe Ala 100 105 110 Gly Tyr Ile Glu Lys Val His Tyr Leu Glu Gln GlnAsn Lys Glu Ile 115 120 125 Glu Ala Glu Ile His Ala Leu Arg Gln Lys GlnAla Ser His Ala Gln 130 135 140 Leu Gly Asp Ala Tyr Asp Gln Glu Ile ArgGlu Leu Arg Ala Thr Leu 145 150 155 160 Glu Met Val Asn His Glu Lys AlaGln Val Gln Leu Asp Ser Asp His 165 170 175 Leu Glu Glu Asp Ile His ArgLeu Lys Glu Arg Phe Glu Glu Glu Ala 180 185 190 Arg Leu Arg Asp Asp ThrGlu Ala Ala Ile Arg Ala Val Arg Lys Asp 195 200 205 Ile Glu Glu Ser SerMet Val Lys Val Glu Leu Asp Lys Lys Val Gln 210 215 220 Ser Leu Gln AspGlu Val Ala Phe Leu Arg Ser Asn His Glu Glu Glu 225 230 235 240 Val AlaAsp Leu Leu Ala Gln Ile Gln Ala Ser His Ile Thr Val Glu 245 250 255 ArgLys Asp Tyr Leu Lys Thr Asp Ile Ser Thr Ala Leu Lys Glu Ile 260 265 270Arg Ser Gln Leu Glu Cys His Ser Asp Gln Asn Met His Gln Ala Glu 275 280285 Glu Trp Phe Lys Cys Arg Tyr Ala Lys Leu Thr Glu Ala Ala Glu Gln 290295 300 Asn Lys Glu Ala Ile Arg Ser Ala Lys Glu Glu Ile Ala Glu Tyr Arg305 310 315 320 Arg Gln Leu Gln Ser Lys Ser Ile Glu Leu Glu Ser Val ArgGly Thr 325 330 335 Lys Glu Ser Leu Glu Arg Gln Leu Ser Asp Ile Glu GluArg His Asn 340 345 350 His Asp Leu Ser Ser Tyr Gln Asp Thr Ile Gln GlnLeu Glu Asn Glu 355 360 365 Leu Arg Gly Thr Lys Trp Glu Met Ala Arg HisLeu Arg Glu Tyr Gln 370 375 380 Asp Leu Leu Asn Val Lys Met Ala Leu AspIle Glu Ile Ala Ala Tyr 385 390 395 400 Arg Lys Leu Leu Glu Gly Glu GluThr Arg Phe Ser Thr Phe Ser Gly 405 410 415 Ser Ile Thr Gly Pro Leu TyrThr His Arg Gln Pro Ser Val Thr Ile 420 425 430 Ser Ser Lys Ile Gln LysThr Lys Val Glu Ala Pro Lys Leu Lys Val 435 440 445 Gln His Lys Phe ValGlu Glu Ile Ile Glu Glu Thr Lys Val Glu Asp 450 455 460 Glu Lys Ser GluMet Glu Asp Ala Leu Thr Val Ile Ala Glu Glu Leu 465 470 475 480 Ala AlaSer Ala Lys Glu Glu Lys Glu Glu Ala Glu Glu Lys Glu Glu 485 490 495 GluPro Glu Val Lys Ser Pro Val Lys Ser Pro Glu Ala Lys Glu Glu 500 505 510Glu Glu Gly Glu Lys Glu Glu Glu Glu Glu Gly Gln Glu Glu Glu Glu 515 520525 Glu Glu Asp Glu Gly Val Lys Ser Asp Gln Ala Glu Glu Gly Gly Ser 530535 540 Glu Lys Glu Gly Ser Ser Glu Lys Asp Glu Gly Glu Gln Glu Glu Glu545 550 555 560 Gly Glu Thr Glu Ala Glu Gly Glu Gly Glu Glu Ala Glu AlaLys Glu 565 570 575 Glu Lys Lys Thr Glu Gly Lys Val Glu Glu Met Ala IleLys Glu Glu 580 585 590 Ile Lys Val Glu Lys Pro Glu Lys Ala Lys Ser ProVal Pro Lys Ser 595 600 605 Pro Val Glu Glu Val Lys Pro Lys Pro Glu AlaLys Ala Gly Lys Asp 610 615 620 Glu Gln Lys Glu Glu Glu Lys Val Glu GluLys Lys Glu Val Ala Lys 625 630 635 640 Glu Ser Pro Lys Glu Glu Lys ValGlu Lys Lys Glu Glu Lys Pro Lys 645 650 655 Asp Val Pro Asp Lys Lys LysAla Glu Ser Pro Val Lys Glu Lys Ala 660 665 670 Val Glu Glu Met Ile ThrIle Thr Lys Ser Val Lys Val Ser Leu Glu 675 680 685 Lys Asp Thr Lys GluGlu Lys Pro Gln Gln Gln Glu Lys Val Lys Glu 690 695 700 Lys Ala Glu GluGlu Gly Gly Ser Glu Glu Glu Val Gly Asp Lys Ser 705 710 715 720 Pro GlnGlu Ser Lys Lys Glu Asp Ile Ala Ile Asn Gly Glu Val Glu 725 730 735 GlyLys Glu Glu Glu Glu Gln Glu Thr Gln Glu Lys Gly Ser Gly Gln 740 745 750Glu Glu Glu Lys Gly Val Val Thr Asn Gly Leu Asp Val Ser Pro Ala 755 760765 Glu Glu Lys Lys Gly Glu Asp Arg Ser Asp Asp Lys Val Val Val Thr 770775 780 Lys Lys Val Glu Lys Ile Thr Ser Glu Gly Gly Asp Gly Ala Thr Lys785 790 795 800 Tyr Ile Thr Lys Ser Val Thr Val Thr Gln Lys Val Glu GluHis Glu 805 810 815 Glu Thr Phe Glu Glu Lys Leu Val Ser Thr Lys Lys ValGlu Lys Val 820 825 830 Thr Ser His Ala Ile Val Lys Glu Val Thr Gln GlyAsp 835 840 845 62 2717 DNA Rattus norvegicus NF-M protein 62 ggcctccaagatgagctaca cgctggactc gctgggcaac ccgtccgcct accggcgcgt 60 tccaaccgagacccggtcca gcttcagtcg tgtgagcggt tccccgtcca gcggcttccg 120 ctcgcagtcctggtcccgcg gctcgcccag caccgtgtcc tcctcctaca agcgcagcgc 180 gctcgccccgcgcctcgcct acagctcggc tatgctcagc tcggccgaga gcagcctcga 240 cttcagccagtcctcttcgc tgcttaacgg cggctccggc ggcgactaca agctgtcccg 300 ctcaaacgagaaagagcagc tgcaggggct gaacgaccgt ttcgccggct acatcgagaa 360 agtgcactacttggaacaac agaacaagga gatcgaggca gagatccacg cgctgcggca 420 gaagcaggcctcgcacgccc agctgggtga cgcttacgac caggagatcc gagagctgcg 480 cgccaccctggagatggtga atcacgagaa ggctcaagtg cagctggact ctgatcactt 540 ggaggaagacatccaccggc tcaaggagcg cttcgaggag gaggcgcggc tgcgggacga 600 cacggaggctgccatccggg cggtgcgcaa agacatagag gagtcgtcga tggttaaggt 660 ggagctggacaagaaggttc agtcgctgca ggatgaggtg gccttcctgc ggagcaatca 720 cgaagaggaggtggccgacc tgctggccca gatccaggcg tcgcacatca ccgtagagcg 780 caaagactacctgaagacag acatctccac ggcgctgaaa gagatccgct cccagctcga 840 gtgtcactccgaccagaaca tgcaccaggc cgaagagtgg ttcaaatgcc gctacgccaa 900 gctcaccgaggcggccgagc agaacaagga ggccatccgc tccgctaaag aagagatcgc 960 cgagtaccggcgccagctgc agtccaagag cattgagctc gagtcggtgc gaggcactaa 1020 ggagtccctggaacggcagc tcagcgacat cgaggagcgc cacaaccacg acctcagcag 1080 ctaccaggacaccatccagc agctggaaaa tgagcttcgg ggaacaaagt gggaaatggc 1140 tcgtcatttgcgagaatacc aggatctcct taacgtcaag atggctctgg acatcgagat 1200 cgccgcatataggaaactac tggagggtga agagaccaga tttagcacat tttcaggaag 1260 catcactgggcctctgtaca cacaccgaca gccctcagtc acaatatcca gtaagattca 1320 gaagaccaaagtcgaggccc ccaagctcaa ggtccaacac aaatttgtgg aggagatcat 1380 tgaggagactaaagtggaag atgagaagtc agaaatggaa gacgccctca cagtcattgc 1440 agaggaattggcagcctctg ccaaagagga gaaagaagag gcagaagaaa aggaagagga 1500 accggaagtgaagtctcccg tgaagtctcc tgaggctaag gaagaggagg aaggggaaaa 1560 ggaggaagaagaggaaggcc aagaggaaga agaggaggaa gatgaaggtg tcaagtcaga 1620 ccaggcagaagagggaggat ctgagaagga aggctcgagt gaaaaggatg aaggtgagca 1680 agaagaagaaggggaaactg aggcagaagg tgaaggagag gaagcagaag ctaaggagga 1740 aaagaaaacagagggaaagg tcgaggaaat ggctatcaag gaggaaatca aggtcgagaa 1800 gcccgagaaagccaagtccc ctgtgccaaa atcaccggtg gaagaagtaa agccaaaacc 1860 agaagccaaagccggaaagg atgagcagaa ggaggaagag aaagttgagg agaagaagga 1920 ggtagccaaggaatcaccca aggaagagaa ggtggagaaa aaggaggaga agccaaaaga 1980 tgtcccagataaaaagaagg ctgagtcccc agtgaaagaa aaggccgtag aggaaatgat 2040 caccattactaagtcggtaa aggtgagcct ggagaaagac accaaagagg agaagcctca 2100 gcagcaggagaaggtgaagg agaaggcaga ggaggagggg ggtagtgagg aggaagtggg 2160 tgacaaaagcccgcaagaat ccaagaagga agacatagct atcaatgggg aggtggaagg 2220 aaaagaggaggaggagcagg aaactcagga gaagggcagt gggcaagagg aggagaaagg 2280 ggtggtcactaatggcttag atgtgagccc tgcggaggaa aagaaagggg aggatagaag 2340 tgatgacaaagtggtggtga ccaagaaggt agaaaaaatc accagcgagg gaggcgatgg 2400 tgctaccaaatacatcacca aatctgttac tgtcactcaa aaggttgaag agcatgagga 2460 gacctttgaggagaagctgg tgtcaactaa aaaggtagaa aaggtcactt cacatgccat 2520 agtcaaggaagtcacccagg gtgactaaga tcggagtcca ttgcaaaagg ttaagccata 2580 caacaatttcaaaatgcatg tgattgacag cttcaaaaca gaatgggttc tcccatgagg 2640 gctccagacattgtattttc ctttgtgcaa tatgagggaa ctgcatgcaa gctcagggtg 2700 cctcctcagtccttggg 2717 63 707 PRT Rattus norvegicus Neurodegeneration associatedprotein 1 63 Met Ser Gln Tyr Thr Glu Lys Glu Pro Ser Val Met Asp Gln AspSer 1 5 10 15 Ser Lys Ala Ala Trp Pro Arg Ala Ala Gly Gly Tyr Gln ThrIle Thr 20 25 30 Gly Arg Arg Tyr Gly Arg Arg His Ala Tyr Val Ser Phe LysPro Cys 35 40 45 Met Thr Arg His Glu Arg Ser Leu Gly Arg Ala Gly Asp AspTyr Glu 50 55 60 Val Leu Glu Leu Asp Asp Val Ala Lys Glu Asn Thr Ala GlySer Ser 65 70 75 80 Ser Leu Asp Gln Val His Pro Ser Leu Pro Ser Glu ThrThr Val Glu 85 90 95 Lys Ser Glu Thr Glu Ile Pro Thr Cys Gly Pro Ala LeuAsn Gln Ser 100 105 110 Thr Glu Ser Asn Pro Ser Val Ala Thr Val Cys HisSer Glu Glu Val 115 120 125 Arg Glu Thr Leu Asp Ser Ser Thr Asn Leu GlnAsn His Ala Glu Arg 130 135 140 Glu Cys Thr Pro Ala Val Cys Asn Ala SerSer Val Gln Asn Gly Ile 145 150 155 160 Val Leu Val His Thr Asp Ser TyrAsp Pro Asp Ser Lys His Asp Glu 165 170 175 Asn Asp Ser Leu Gln Leu CysAla Gln Ala Val Glu Gly Gly Arg Arg 180 185 190 Gln Lys Val Leu Gly AsnAla Val Phe Glu Leu Glu Asn Gly Glu Val 195 200 205 Glu Arg Tyr Ala AspLeu Cys Pro Ser Val Pro Ser Leu Ser Gly Glu 210 215 220 Ile Arg Glu GluSer Glu Glu Leu Gly Ser Ala Leu Leu Glu Lys Asn 225 230 235 240 Ser AlaGly Asp Ala Glu Ala Val His Gln Asp Gly Gln Glu Phe Gln 245 250 255 ArgSer Ser Glu Asp Gly Ile Val Arg Lys Arg Arg Gln Asp Asp Thr 260 265 270Asp Gln Gly Arg Gln Thr Glu Asn Ser Thr Glu Asp Ala Asp Cys Val 275 280285 Pro Gly His Val Glu Gln Asn Thr Ser Glu Arg Ala Asn His His Gly 290295 300 Ser Ser Pro Glu Gln Val Val Arg Pro Lys Val Arg Lys Val Ile Ser305 310 315 320 Ser Ser Gln Val Asp Gln Glu Ser Gly Phe Asn Arg His GluAla Lys 325 330 335 Gln Arg Ser Val Gln Arg Trp Arg Glu Ala Leu Glu ValGlu Glu Cys 340 345 350 Ser Ser Asp Asp Pro Ile Ile Lys Cys Asp Asp TyrAsp Gly Asp His 355 360 365 Asp Cys Met Phe Leu Thr Pro Ser Tyr Ser ArgVal Thr Pro Arg Glu 370 375 380 Ala Glu Arg His Arg Ala Thr Ala Glu AsnGly Ala Thr Ala Ser Gly 385 390 395 400 Arg Gln Glu Ala Arg Glu Asn AlaPhe Trp Asn Ala Cys Gly Glu Tyr 405 410 415 Tyr Gln Leu Phe Asp Lys AspGlu Asp Ser Ser Glu Cys Ser Asp Gly 420 425 430 Glu Trp Ser Ala Ser LeuPro His Arg Phe Ser Gly Thr Glu Lys Asp 435 440 445 Gln Ser Ser Ser AspGlu Ser Trp Glu Thr Leu Pro Gly Lys Asp Glu 450 455 460 Asn Glu Pro GluLeu Gln Ser Asp Ser Ser Gly Pro Glu Glu Glu Asn 465 470 475 480 Gln GluLeu Ser Leu Gln Glu Gly Glu Gln Thr Ser Leu Glu Glu Gly 485 490 495 GluIle Pro Trp Leu Gln Tyr Asn Glu Val Asn Glu Ser Ser Ser Asp 500 505 510Glu Gly Asn Glu Pro Ala Asn Glu Phe Ala Gln Pro Glu Ala Phe Met 515 520525 Leu Asp Gly Asn Asn Asn Leu Glu Asp Asp Ser Ser Val Ser Glu Asp 530535 540 Leu Asp Val Asp Trp Ser Leu Phe Asp Gly Phe Ala Asp Gly Leu Gly545 550 555 560 Val Ala Glu Ala Ile Ser Tyr Val Asp Pro Gln Phe Leu ThrTyr Met 565 570 575 Ala Leu Glu Glu Arg Leu Ala Gln Ala Met Glu Thr AlaLeu Ala His 580 585 590 Leu Glu Ser Leu Ala Val Asp Val Glu Val Ala AsnPro Pro Ala Ser 595 600 605 Lys Glu Ser Ile Asp Gly Leu Pro Glu Thr LeuVal Leu Glu Asp His 610 615 620 Thr Ala Ile Gly Gln Glu Gln Cys Cys ProIle Cys Cys Ser Glu Tyr 625 630 635 640 Ile Lys Asp Asp Ile Ala Thr GluLeu Pro Cys His His Phe Phe His 645 650 655 Lys Pro Cys Val Ser Ile TrpLeu Gln Lys Ser Gly Thr Cys Pro Val 660 665 670 Cys Arg Arg His Phe ProPro Ala Val Ile Asp Ala Ser Ala Ala Ala 675 680 685 Ser Ser Glu Pro AspLeu Asp Ala Ser Pro Ala Asn Asp Asn Ala Glu 690 695 700 Glu Ala Pro 70564 4758 DNA Rattus norvegicus Neurodegeneration associated protein 1 64tggcgggtgg aggatcgagc gctgttctcg ctctggagcc gctgcacatc tcggaatcat 60ttgcagctta ttcactgtgt aaggaaaaac caaattacat ccataattct attgaactgg 120aagcatagac gctgccatat atgtcacagt atacagaaaa agaaccatca gtaatggatc 180aagactccag caaggctgcg tggcctagag cagcaggagg ataccagacg attacaggca 240ggaggtacgg aaggagacat gcgtatgtca gttttaagcc atgtatgacc aggcatgaac 300ggagcttggg tcgggctggc gatgactatg aagttctgga actggatgac gttgccaagg 360aaaacaccgc aggttccagt tcattggatc aagtccatcc ttctttaccc agtgaaacta 420cagttgaaaa aagtgaaaca gaaattccta cttgtggtcc agcactgaat caaagcacgg 480agagcaaccc atccgttgcc acagtgtgtc atagtgagga agtcagggag accttagaca 540gcagtacgaa tcttcagaat cacgctgaga gagagtgtac gccagcagtt tgtaatgcct 600caagtgtcca gaatggaatt gtgttggttc atactgactc ttatgatcca gacagcaagc 660atgatgagaa tgactctctt caactttgtg cccaagctgt ggaaggtggt agacgtcaga 720aggtattagg caatgcagtc tttgagctgg aaaatggaga ggtagagaga tatgctgatc 780tgtgtccctc agttccctct ctcagtggtg aaataaggga ggagtctgaa gagctaggtt 840cagcactctt agagaaaaat tctgctggcg atgcggaggc tgtccatcag gatgggcagg 900aatttcagag gtcttctgaa gacggcattg ttagaaagag gcgacaagat gataccgatc 960agggaagaca gacagaaaat tcaactgaag atgcagactg tgttccaggc catgttgaac 1020aaaatactag tgagagagcc aatcaccatg gaagttctcc tgaacaggta gtgaggccca 1080aagttagaaa agtgatcagt tcaagccagg tggaccaaga gagcggtttt aataggcacg 1140aggctaagca aagaagtgtt cagaggtgga gagaggctct ggaagttgag gaatgcagtt 1200cagatgaccc tataatcaag tgtgacgatt atgatggaga ccatgactgc atgttcctaa 1260ccccatccta ctcaagagtt acgccaaggg aagcagaacg tcaccgtgcg acagcagaaa 1320atggagccac agcttcagga aggcaagagg ctcgggaaaa tgccttttgg aatgcctgtg 1380gagagtatta ccagctcttt gacaaagacg aagacagttc agagtgcagt gatggggaat 1440ggtctgcttc tctgcctcac cgattttctg gcacagaaaa agaccagtcc tcaagcgatg 1500aaagctggga aactctgcca ggaaaagatg agaatgaacc tgagctacag agtgatagta 1560gtggccctga ggaagaaaac caagaattgt ctcttcagga gggagaacag acgtccttgg 1620aggaggggga gattccctgg ttacagtaca atgaggtcaa tgagagcagc agcgatgaag 1680ggaacgagcc tgccaatgag tttgcacaac cagaagcttt catgttggat gggaacaaca 1740acctggagga cgactcgagc gtgagtgaag acctggatgt ggactggagc ctatttgatg 1800gttttgcgga tggacttggt gttgctgaag ctatttccta cgtggatcct cagttcctca 1860cctacatggc actagaagaa cgcttagccc aggctatgga gactgcactg gcacacttag 1920agtctcttgc tgtagacgtt gaggtggcta acccacctgc cagtaaggaa agcatcgacg 1980gacttccaga aacccttgtc ctagaagatc acaccgctat tggtcaggag cagtgctgtc 2040ccatctgctg cagtgagtac attaaggatg acatcgcaac agagctgccc tgccatcact 2100tctttcataa gccttgtgtc tccatttggc tacagaagtc cggaacgtgc cctgtgtgcc 2160gccgccactt cccacctgca gtgattgacg catctgcagc tgcttcctct gaaccagacc 2220ttgatgcctc gcctgcaaac gacaatgctg aggaagcccc gtaagccgtc acggggaaat 2280gagatcgaaa ttctatcaca gtaaatctgc aaattccttc taaatctgat gtgcaaataa 2340ttatatataa atatatttaa aatgctctat atagtatatg ccgtagttta gaaagagaat 2400attaaccttt ctaaactgaa tttaggtttg cagaagatac taaacatttt caagctgaat 2460gttgaagcag tgcatccatt ttctttagtt gaacatggtg tccattgtaa cgtcagtagt 2520catctctgtg gcatacgttt ctgttttgtc tgaagtgctg ccactaagag atcagaatta 2580agctctcctt tccacatcag atgtgaaagg agagcttaat tctgatctct gtggcatacg 2640tttctgtttt gtctgaagtg ctgccactaa gagatcagaa ttaagctctc ctttccacat 2700cagatgtgaa aactgtgatc acaacagtag tacagtttgg tttcattgaa aataaactga 2760attctaaagc atgctttttc actggtccct ttgcttttgc tacttcgaga cctcttggtt 2820tatataacac tgaggttaag attaaagctt ttcagaatgc caggcaaaga ctagagtttt 2880gacccgaaca cacacacaaa ataaaaaaat aaaaaataaa ttacatctta gatgtaaact 2940tcctgaaact gtcatgtaat aaactattca gtgaacgttg gatgtttaaa ggctagtaaa 3000gcatgtgaat tatctgatgt gcttcatgct cggcacagtc tcatgagctt ggcctatgaa 3060acactgtcta agccctcatg cagtctgctt ctacttgtta aacactgaag gtccagacca 3120gtcttggcat ctgattaaga aattggatgt agttagctaa ggtagtttgg gttctacttt 3180aggagatctg aagatttgca catggccatg tctgttacag catcacccca gattagaaag 3240gcacagcttt taggtggtca cacgagtagg caagtctgtg cagacgctgg tgtgataggt 3300agcttacagg tgagccctga catcagcaca gggacaacgg ctcagcaggc ccagcactgg 3360agcccatccg cgtgcacatc tacctgccct gctcatccag ttcgggaata tttctatagc 3420ttcccaaatc agcttcatct gatttgtgta atatatctga gtatttatag tctttctttt 3480ttctccagtc ttaaaaataa atgaattttc actgttggca catttgaggc ttaaatataa 3540gaaacctaac acttgcattc tgatttttgc atatattgta aatgtggctg gtatttacag 3600cgaatcctgt gtgtcctttt atgggtaaaa caaaagtgaa cattgcatgc aagtaatgtg 3660gtgaatttgt aatttagggg ttttctgggg cttctgtgac tgtggatgct taaattcagg 3720ccttaatgtt gctctagcta gcatgtttcc ttctaatgta catagtcagc ctgtatccac 3780taatctctgc ttgtcttctc ctccgtggtc ttcacaccgt atccactgat ggtcagttag 3840tgtcagaggt cacccaggtt agcatcagtt cccatgtgtg ccgtgcggat tgtgagccgt 3900gtggttgctt ggttggtttt cttttatgta ctttgattag ctgtggcact tactgagtaa 3960acttacgttg ctgcagactg ctgtgtaaca agccacgttt tgtgtttagt cagtattaca 4020cacgggacct tgttttattc ttctagatag cagctgtccc aaagaaaata tttcttcttt 4080gcctattaag atttagctat tatctgccag ttgttaaggg gtttgattcc aaactcaacc 4140agagttgaac gtaagaaagc tgctgtactc ttgctgtcca tctgttacgt cctccgttgt 4200tggctcctga ctacgtaccc acggctgctg aaggagactt gttttaattt gttttaaaaa 4260ggaaaatgaa gttttaggac tttgatggtg gggaagctga caagttcaga ccccagtctg 4320tcatttgctc ttagcttgga tccttttgaa aagttaagaa tatatgaagg taaattagaa 4380aaagtaaaaa tattgataaa atgtcattta tcttatgtct attttatgtt gttatttggc 4440ctattaattt taattttttg aacattttct tatttcttgt aatatgaatg ccgatattta 4500aagtttgact cacttgggtt tcttttgtgt ttcttagttg ttatctctaa gcctaactaa 4560cttttaagat tatttgtgat atggatttat ataagctgtt aaatatatat gagctgttaa 4620aatggaatgc aaggttttcc aaagaccagg tctaactgta atgattggtt tattgttcta 4680caatcccagc ccggcatttt ccgtgtaaat cataaacaat aaacaggata tactcagtgt 4740tcatttctaa aaaaaaaa 4758 65 1488 DNA Rattus norvegicus Brain S-100protein beta-subunit 65 aagtccacac ccagtcctct ctggaggaag aaaagggagcttctctgtct accctcctag 60 tccttggaca ccgaagccag agaggactcc ggcggcaaaaggtgaccagg agcctccggg 120 atgtctgagc tggagaaggc catggttgcc ctcattgatgtcttccatca gtattcaggg 180 agagagggtg acaagcacaa gctgaagaag tcagaactgaaggagctcat caacaacgag 240 ctctctcact tcctggagga aatcaaagag caggaagtggtggacaaagt gatggagacg 300 ctggacgaag atggggatgg ggagtgtgac ttccaggagtttatggcctt cgtctccatg 360 gtgaccacag cctgtcatga gttctttgaa catgagtgagacaaaaaaaa aaaaaaaaaa 420 gtggctgagc cgtttccccg tggcagacat gagggccacgagaggaggca cggcagaagg 480 ctcgtgggct ggaaggagct gcgctctcta gacgcatataactaattagg aagcttgatt 540 tgcttcaggg atgaaactct gaccccgttc ccaagggctgctttaagtta gcacttcgtt 600 tctgctacac taggtattcc tgtgagctga atggtcccgggaactattga taagagtcac 660 tgagggacga aatcaacact ctgtgggtat agcactggttgtagaccacc atgctcctgt 720 ggaagggtca cctgtaagaa tcaaggcaga ctaccaatagcacctccgtt ggacagcttt 780 cttaggtgta atgtatgctg tccatgcatc tacagacccacagctggatc cactgccacc 840 cgaagaggtt ggctcgccct tacaactgct tgtcctctgtgcaaacgatg ccccggaaag 900 ttagacctat cacccacacc ctcccaccac ccccaggccaaaaggacagc ccacccaagt 960 cccctccccc acagcgaatc gcggtttgtt accaagtacgtatttgacat caacagttcc 1020 aactggtgga acgattagat ctcgcacact aagtattagcaccctaactc acgaccgaga 1080 atcaaaattc tgctcagtag acgtctcctt tcaggatgacaccattgtcc ccataggaca 1140 cggacagagg agggcactgg agagagtgtc aggtctttttctagctgtat cttcctctct 1200 ccctctgctg cccataatgt gagtgaccct ctagggtgagacttgcaggg tgagctgctg 1260 aggaatgaag ggccactgag atgtgtcctt tagctgctgggtgtcatgtc tgacctgctg 1320 gtgcctaggg cctgcttaac actcggcaag gctgcgagccgaggactgtg ggaagccgga 1380 cttgatgctt tctaacctgc atatttgaat gccgaaggtcaaacaatcca agttacagat 1440 aaataaaaac cgcattgcaa gtattaaaaa gccattctaggaaaattc 1488 66 2364 PRT Rattus norvegicus Microtubule associatedprotein IB 66 Gly Gln Lys Ile Leu His His Arg Ser Asp Val Leu Glu ThrVal Val 1 5 10 15 Leu Ile Asn Pro Ser Asp Glu Ala Val Ser Thr Glu ValArg Leu Met 20 25 30 Ile Thr Asp Ala Ala Arg His Lys Leu Leu Val Leu ThrGly Gln Cys 35 40 45 Phe Glu Asn Thr Gly Glu Leu Ile Leu Gln Ser Gly SerPhe Ser Phe 50 55 60 Gln Asn Phe Ile Glu Ile Phe Thr Asp Gln Glu Ile GlyGlu Leu Leu 65 70 75 80 Ser Thr Thr His Pro Ala Asn Lys Ala Ser Leu ThrLeu Phe Cys Pro 85 90 95 Glu Glu Gly Asp Trp Lys Asn Ser Asn Leu Asp ArgHis Asn Leu Gln 100 105 110 Asp Phe Ile Asn Ile Lys Leu Asn Ser Ala SerIle Leu Pro Glu Met 115 120 125 Glu Gly Leu Ser Glu Phe Thr Glu Tyr LeuSer Glu Ser Val Glu Val 130 135 140 Pro Ser Pro Phe Asp Ile Leu Glu ProPro Thr Ser Gly Gly Phe Leu 145 150 155 160 Lys Leu Ser Lys Pro Cys CysTyr Ile Phe Pro Gly Gly Arg Gly Asp 165 170 175 Ser Ala Leu Phe Ala ValAsn Gly Phe Asn Met Leu Ile Asn Gly Gly 180 185 190 Ser Glu Arg Lys SerCys Phe Trp Lys Leu Ile Arg His Leu Asp Arg 195 200 205 Val Asp Ser IleLeu Leu Thr His Ile Gly Asp Asp Asn Leu Pro Gly 210 215 220 Ile Asn SerMet Leu Gln Arg Lys Ile Ala Glu Leu Glu Glu Glu Ser 225 230 235 240 GlnGly Ser Thr Ser Asn Ser Asp Trp Met Lys Asn Leu Ile Ser Pro 245 250 255Asp Leu Gly Val Val Phe Leu Asn Val Pro Glu Asn Leu Lys Asn Pro 260 265270 Glu Pro Asn Ile Lys Met Lys Arg Ser Thr Glu Glu Ala Cys Phe Thr 275280 285 Leu Gln Tyr Leu Asn Lys Leu Ser Met Lys Pro Glu Pro Leu Phe Arg290 295 300 Ser Val Gly Asn Ala Ile Glu Pro Val Ile Leu Phe Gln Lys MetGly 305 310 315 320 Val Gly Lys Leu Lys Met Tyr Val Leu Asn Pro Val LysSer Ser Lys 325 330 335 Glu Met Gln Tyr Phe Met Gln Gln Trp Thr Gly ThrAsn Lys Asp Lys 340 345 350 Ala Glu Leu Ile Leu Pro Asn Gly Gln Glu ValAsp Ile Pro Ile Ser 355 360 365 Tyr Leu Ala Ser Val Ser Ser Leu Ile ValTrp His Pro Ala Asn Pro 370 375 380 Ala Glu Lys Ile Ile Arg Val Leu PhePro Gly Asn Ser Thr Gln Tyr 385 390 395 400 Asn Ile Leu Glu Gly Leu GluLys Leu Lys His Leu Asp Phe Leu Lys 405 410 415 Gln Pro Leu Ala Thr GlnLys Asp Leu Thr Gly Gln Val Ser Thr Pro 420 425 430 Pro Val Lys Gln ValLys Leu Lys Gln Arg Ala Asp Ser Arg Glu Ser 435 440 445 Leu Lys Pro AlaThr Lys Pro Leu Ser Ser Lys Ser Val Arg Lys Glu 450 455 460 Ser Lys GluGlu Ala Pro Glu Ala Thr Lys Ala Ser Gln Val Glu Lys 465 470 475 480 ThrPro Lys Val Glu Ser Lys Glu Lys Val Ile Val Lys Lys Asp Lys 485 490 495Pro Gly Lys Val Glu Ser Lys Pro Ser Val Thr Glu Lys Glu Val Pro 500 505510 Ser Lys Glu Glu Gln Ser Pro Val Lys Ala Glu Val Ala Glu Lys Ala 515520 525 Ala Thr Glu Ser Lys Pro Lys Val Thr Lys Asp Lys Val Val Lys Lys530 535 540 Glu Ile Lys Thr Lys Pro Glu Glu Lys Lys Glu Glu Lys Pro LysLys 545 550 555 560 Glu Val Ala Lys Lys Glu Asp Lys Thr Pro Leu Lys LysAsp Glu Lys 565 570 575 Pro Lys Lys Glu Glu Ala Lys Lys Glu Ile Lys LysGlu Ile Lys Lys 580 585 590 Glu Glu Lys Lys Glu Leu Lys Lys Glu Val LysLys Glu Thr Pro Leu 595 600 605 Lys Asp Ala Lys Lys Glu Val Lys Lys AspGlu Lys Lys Glu Val Lys 610 615 620 Lys Glu Glu Lys Glu Pro Lys Lys GluIle Lys Lys Ile Ser Lys Asp 625 630 635 640 Ile Lys Lys Ser Thr Pro LeuSer Asp Thr Lys Lys Pro Ala Ala Leu 645 650 655 Lys Pro Lys Val Ala LysLys Glu Glu Pro Thr Lys Lys Glu Pro Ile 660 665 670 Ala Ala Gly Lys LeuLys Asp Lys Gly Lys Val Lys Val Ile Lys Lys 675 680 685 Glu Gly Lys ThrThr Glu Ala Ala Ala Thr Ala Val Gly Thr Ala Ala 690 695 700 Val Ala AlaAla Ala Gly Val Ala Ala Ser Gly Pro Ala Lys Glu Leu 705 710 715 720 GluAla Glu Arg Ser Leu Met Ser Ser Pro Glu Asp Leu Thr Lys Asp 725 730 735Phe Glu Glu Leu Lys Ala Glu Glu Ile Asp Val Ala Lys Asp Ile Lys 740 745750 Pro Gln Leu Glu Leu Ile Glu Asp Glu Glu Lys Leu Lys Glu Thr Glu 755760 765 Pro Gly Glu Ala Tyr Val Ile Gln Lys Glu Thr Glu Val Ser Lys Gly770 775 780 Ser Ala Glu Ser Pro Asp Glu Gly Ile Thr Thr Thr Glu Gly GluGly 785 790 795 800 Glu Cys Glu Gln Thr Pro Glu Glu Leu Glu Pro Val GluLys Gln Gly 805 810 815 Val Asp Asp Ile Glu Lys Phe Glu Asp Glu Gly AlaGly Phe Glu Glu 820 825 830 Ser Ser Glu Ala Gly Asp Tyr Glu Glu Lys AlaGlu Thr Glu Glu Ala 835 840 845 Glu Glu Pro Glu Glu Asp Gly Glu Asp AsnVal Ser Gly Ser Ala Ser 850 855 860 Lys His Ser Pro Thr Glu Asp Glu GluIle Ala Lys Ala Glu Ala Asp 865 870 875 880 Val His Ile Lys Glu Lys ArgGlu Ser Val Ala Ser Gly Asp Asp Arg 885 890 895 Ala Glu Glu Asp Met AspGlu Ala Leu Glu Lys Gly Glu Ala Glu Gln 900 905 910 Ser Glu Glu Glu GlyGlu Glu Glu Glu Asp Lys Ala Glu Asp Ala Arg 915 920 925 Glu Glu Asp HisGlu Pro Asp Lys Thr Glu Ala Glu Asp Tyr Val Met 930 935 940 Ala Val ValAsp Lys Ala Ala Glu Ala Gly Val Thr Glu Asp Gln Tyr 945 950 955 960 AspPhe Leu Gly Thr Pro Ala Lys Gln Pro Gly Val Gln Ser Pro Ser 965 970 975Arg Glu Pro Ala Ser Ser Ile His Asp Glu Thr Leu Pro Gly Gly Ser 980 985990 Glu Ser Glu Ala Thr Ala Ser Asp Glu Glu Asn Arg Glu Asp Gln Pro 9951000 1005 Glu Glu Phe Thr Ala Thr Ser Gly Tyr Thr Gln Ser Thr Ile GluIle 1010 1015 1020 Ser Ser Glu Pro Thr Pro Met Asp Glu Met Ser Thr ProArg Asp Val 1025 1030 1035 1040 Met Thr Asp Glu Thr Asn Asn Glu Glu ThrGlu Ser Pro Ser Gln Glu 1045 1050 1055 Phe Val Asn Ile Thr Lys Tyr GluSer Ser Leu Tyr Ser Gln Glu Tyr 1060 1065 1070 Ser Lys Pro Val Val AlaSer Phe Asn Gly Leu Ser Asp Gly Ser Lys 1075 1080 1085 Thr Asp Ala ThrAsp Gly Arg Asp Tyr Asn Ala Ser Ala Ser Thr Ile 1090 1095 1100 Ser ProPro Ser Ser Met Glu Glu Asp Lys Phe Ser Lys Ser Ala Leu 1105 1110 11151120 Arg Asp Ala Tyr Arg Pro Glu Glu Thr Asp Val Lys Thr Gly Ala Glu1125 1130 1135 Leu Asp Ile Lys Asp Val Ser Asp Glu Arg Leu Ser Pro AlaLys Ser 1140 1145 1150 Pro Ser Leu Ser Pro Ser Pro Pro Ser Pro Ile GluLys Thr Pro Leu 1155 1160 1165 Gly Glu Arg Ser Val Asn Phe Ser Leu ThrPro Asn Glu Ile Lys Ala 1170 1175 1180 Ser Ala Glu Gly Glu Ala Thr AlaVal Val Ser Pro Gly Val Thr Gln 1185 1190 1195 1200 Ala Val Val Glu GluHis Cys Ala Ser Pro Glu Glu Lys Thr Leu Glu 1205 1210 1215 Val Val SerPro Ser Gln Ser Val Thr Gly Ser Ala Gly His Thr Pro 1220 1225 1230 TyrTyr Gln Ser Pro Thr Asp Glu Lys Ser Ser His Leu Pro Thr Glu 1235 12401245 Val Thr Glu Asn Ala Gln Ala Val Pro Val Ser Phe Glu Phe Thr Glu1250 1255 1260 Ala Lys Asp Glu Asn Glu Arg Ser Ser Ile Ser Pro Met AspGlu Pro 1265 1270 1275 1280 Val Pro Asp Ser Glu Ser Pro Ile Glu Lys ValLeu Ser Pro Leu Arg 1285 1290 1295 Ser Pro Pro Leu Ile Gly Ser Glu SerAla Tyr Glu Asp Phe Leu Ser 1300 1305 1310 Ala Asp Asp Lys Ala Leu GlyArg Arg Ser Glu Ser Pro Phe Glu Gly 1315 1320 1325 Lys Asn Gly Lys GlnGly Phe Ser Asp Lys Glu Ser Pro Val Ser Asp 1330 1335 1340 Leu Thr SerAsp Leu Tyr Gln Asp Lys Gln Glu Glu Lys Arg Ala Gly 1345 1350 1355 1360Phe Ile Pro Ile Lys Glu Asp Phe Ser Pro Glu Lys Lys Ala Ser Asp 13651370 1375 Ala Glu Ile Met Ser Ser Gln Ser Ala Leu Ala Leu Asp Glu ArgLys 1380 1385 1390 Leu Gly Gly Asp Gly Ser Pro Thr Gln Val Asp Val SerGln Phe Gly 1395 1400 1405 Ser Phe Lys Glu Asp Thr Lys Met Ser Ile SerGlu Gly Thr Val Ser 1410 1415 1420 Asp Lys Ser Ala Thr Pro Val Asp GluGly Ala Glu Asp Thr Tyr Ser 1425 1430 1435 1440 His Met Glu Gly Val AlaSer Val Ser Thr Ala Ser Val Ala Thr Ser 1445 1450 1455 Ser Phe Pro GluPro Thr Thr Asp Asp Val Ser Pro Ser Leu His Ala 1460 1465 1470 Glu ValGly Ser Pro His Ser Thr Glu Val Asp Asp Ser Leu Ser Val 1475 1480 1485Ser Val Val Gln Thr Pro Thr Thr Phe Gln Glu Thr Glu Met Ser Pro 14901495 1500 Ser Lys Glu Glu Cys Pro Arg Pro Met Ser Ile Ser Pro Pro AspPhe 1505 1510 1515 1520 Ser Pro Lys Thr Ala Lys Ser Arg Thr Pro Val GlnAsp His Arg Ser 1525 1530 1535 Glu Gln Ser Ser Met Ser Ile Glu Phe GlyGln Glu Ser Pro Glu His 1540 1545 1550 Ser Leu Ala Met Asp Phe Ser ArgGln Ser Pro Asp His Pro Thr Val 1555 1560 1565 Gly Ala Gly Met Leu HisIle Thr Glu Asn Gly Pro Thr Glu Val Asp 1570 1575 1580 Tyr Ser Pro SerAsp Ile Gln Asp Ser Ser Leu Ser His Lys Ile Pro 1585 1590 1595 1600 ProThr Glu Glu Pro Ser Tyr Thr Gln Asp Asn Asp Leu Ser Glu Leu 1605 16101615 Ile Ser Val Ser Gln Val Glu Ala Ser Pro Ser Thr Ser Ser Ala His1620 1625 1630 Thr Pro Ser Gln Ile Ala Ser Pro Leu Gln Glu Asp Thr LeuSer Asp 1635 1640 1645 Val Val Pro Pro Arg Asp Met Ser Leu Tyr Ala SerLeu Ala Ser Glu 1650 1655 1660 Lys Val Gln Ser Leu Glu Gly Glu Lys LeuSer Pro Lys Ser Asp Ile 1665 1670 1675 1680 Ser Pro Leu Thr Pro Arg GluSer Ser Pro Thr Tyr Ser Pro Gly Phe 1685 1690 1695 Ser Asp Ser Thr SerGly Ala Lys Glu Ser Thr Ala Ala Tyr Gln Thr 1700 1705 1710 Ser Ser SerPro Pro Ile Asp Ala Ala Ala Ala Glu Pro Tyr Gly Phe 1715 1720 1725 ArgSer Ser Met Leu Phe Asp Thr Met Gln His His Leu Ala Leu Ser 1730 17351740 Arg Asp Leu Thr Thr Ser Ser Val Glu Lys Asp Asn Gly Gly Lys Thr1745 1750 1755 1760 Pro Gly Asp Phe Asn Tyr Ala Tyr Gln Lys Pro Glu SerThr Thr Glu 1765 1770 1775 Ser Pro Asp Glu Glu Asp Tyr Asp Tyr Glu SerHis Glu Lys Thr Ile 1780 1785 1790 Gln Ala His Asp Val Gly Gly Tyr TyrTyr Glu Lys Thr Glu Arg Thr 1795 1800 1805 Ile Lys Ser Pro Cys Asp SerGly Tyr Ser Tyr Glu Thr Ile Glu Lys 1810 1815 1820 Thr Thr Lys Thr ProGlu Asp Gly Gly Tyr Ser Cys Glu Ile Thr Glu 1825 1830 1835 1840 Lys ThrThr Arg Thr Pro Glu Glu Gly Gly Tyr Ser Tyr Glu Ile Ser 1845 1850 1855Glu Lys Thr Thr Arg Thr Pro Glu Val Ser Gly Tyr Thr Tyr Glu Lys 18601865 1870 Thr Glu Arg Ser Arg Arg Leu Leu Asp Asp Ile Ser Asn Gly TyrAsp 1875 1880 1885 Asp Thr Glu Asp Gly Gly His Thr Leu Gly Asp Cys SerTyr Ser Tyr 1890 1895 1900 Glu Thr Thr Glu Lys Ile Thr Ser Phe Pro GluSer Glu Ser Tyr Ser 1905 1910 1915 1920 Tyr Glu Thr Thr Thr Lys Thr ThrArg Ser Pro Asp Thr Ser Ala Tyr 1925 1930 1935 Cys Tyr Glu Thr Met GluLys Ile Thr Lys Thr Pro Gln Ala Ser Thr 1940 1945 1950 Tyr Ser Tyr GluThr Ser Asp Arg Cys Tyr Thr Pro Glu Arg Lys Ser 1955 1960 1965 Pro SerGlu Ala Arg Gln Asp Val Asp Leu Cys Leu Val Ser Ser Cys 1970 1975 1980Glu Phe Lys His Pro Lys Thr Glu Leu Ser Pro Ser Phe Ile Asn Pro 19851990 1995 2000 Asn Pro Leu Glu Trp Phe Ala Gly Glu Glu Pro Thr Glu GluSer Glu 2005 2010 2015 Arg Pro Leu Thr Gln Ser Gly Gly Ala Pro Pro ProSer Gly Gly Lys 2020 2025 2030 Gln Gln Gly Arg Gln Cys Asp Glu Thr ProPro Thr Ser Val Ser Glu 2035 2040 2045 Ser Ala Pro Ser Gln Thr Asp SerAsp Val Pro Pro Glu Thr Glu Glu 2050 2055 2060 Cys Pro Ser Ile Thr AlaAsp Ala Asn Leu Asp Ser Glu Asp Glu Ser 2065 2070 2075 2080 Glu Thr IlePro Thr Asp Lys Thr Val Thr Tyr Lys His Met Asp Pro 2085 2090 2095 ProPro Ala Pro Met Gln Asp Arg Ser Pro Ser Pro Arg His Pro Asp 2100 21052110 Val Ser Met Val Asp Pro Glu Ala Leu Ala Ile Glu Gln Asn Leu Gly2115 2120 2125 Lys Ala Leu Lys Lys Asp Leu Lys Glu Lys Ala Lys Thr LysLys Pro 2130 2135 2140 Gly Thr Lys Thr Lys Ser Ser Ser Pro Val Lys LysGly Asp Gly Lys 2145 2150 2155 2160 Ser Lys Pro Ser Ala Ala Ser Pro LysPro Gly Ala Leu Lys Glu Ser 2165 2170 2175 Ser Asp Lys Val Ser Arg ValAla Ser Pro Lys Lys Lys Glu Ser Val 2180 2185 2190 Glu Lys Ala Met LysThr Thr Thr Thr Pro Glu Val Lys Ala Thr Arg 2195 2200 2205 Gly Glu GluLys Asp Lys Glu Thr Lys Asn Ala Ala Asn Ala Ser Ala 2210 2215 2220 SerLys Ser Val Lys Thr Ala Thr Ala Gly Pro Gly Thr Thr Lys Thr 2225 22302235 2240 Ala Lys Ser Ser Thr Val Pro Pro Gly Leu Pro Val Tyr Leu AspLeu 2245 2250 2255 Cys Tyr Ile Pro Asn His Ser Asn Ser Lys Asn Val AspVal Glu Phe 2260 2265 2270 Phe Lys Arg Val Arg Ser Ser Tyr Tyr Val ValSer Gly Asn Asp Pro 2275 2280 2285 Ala Ala Glu Glu Pro Ser Arg Ala ValLeu Asp Ala Leu Leu Glu Gly 2290 2295 2300 Lys Ala Gln Trp Gly Ser AsnMet Gln Val Thr Leu Ile Pro Thr His 2305 2310 2315 2320 Asp Ser Glu ValMet Arg Glu Trp Tyr Gln Glu Thr His Glu Lys Gln 2325 2330 2335 Gln AspLeu Asn Ile Met Val Leu Ala Ser Ser Ser Thr Val Val Met 2340 2345 2350Gln Asp Glu Ser Phe Pro Ala Cys Lys Ile Glu Leu 2355 2360 67 7095 DNARattus norvegicus Microtubule associated protein IB 67 ggacaaaagatcctccatca ccgaagtgac gtcttagaaa ctgtagttct gatcaaccct 60 tcggatgaagcagtcagcac cgaggtgcgt ttgatgatca ctgacgccgc ccgccataaa 120 ctgctggtgctcaccggaca gtgctttgag aacactggag agctcatcct ccagtcaggc 180 tctttctccttccagaactt catagagatt ttcaccgacc aagagattgg ggagctccta 240 agcaccacccatcctgccaa caaagccagc ctcaccctct tctgccctga ggaaggagac 300 tggaagaactccaaccttga cagacacaat ctccaagact tcatcaacat caagctcaac 360 tcagcttctatcttgccaga aatggaggga ctttctgagt tcaccgagta cctctcggag 420 tctgtcgaagtcccctcccc ctttgacatc ctggagcccc cgacctcggg cggatttctg 480 aagctctccaagccttgttg ttacattttt ccggggggcc gcggggactc tgccctgttc 540 gcagtgaacggattcaacat gctcattaac ggaggatcag aaagaaagtc ctgcttctgg 600 aagctcattcggcacttgga ccgggtggac tccatcctgc tcacccacat tggggatgac 660 aacttgcccgggatcaacag catgttgcaa cgcaagattg cagagctgga agaggagtcc 720 cagggctccaccagcaacag tgactggatg aaaaacctca tctcccctga cttgggggtt 780 gtgtttctcaatgtacctga aaatctgaaa aacccagaac ccaacatcaa gatgaagaga 840 agtacagaagaagcatgctt caccctccag tacctaaaca aactgtccat gaaaccagag 900 cctttatttagaagtgtagg caatgccatt gagcctgtca tcctgttcca aaaaatggga 960 gtgggtaaactgaagatgta tgtgcttaac ccagtcaaaa gcagcaagga aatgcagtat 1020 ttcatgcagcagtggactgg aaccaacaaa gacaaggctg aacttatcct gcccaatggt 1080 caagaagtagacatcccgat ttcctaccta gcttccgtct cgtctttgat tgtgtggcac 1140 ccagccaaccctgctgagaa aatcatccgg gttctgtttc ctggaaacag cacccagtac 1200 aacatcctagaagggctgga aaaactcaaa catctagact tcctaaagca gccactggcc 1260 acccaaaaagatctcactgg ccaggtgtcc acccccccag tgaaacaggt caagttgaaa 1320 cagcgggctgacagccgaga gagtctgaag ccagccacaa aaccactttc cagtaaatca 1380 gtgaggaaggagtccaaaga ggaggcccct gaagccacaa aagccagcca agtggaaaaa 1440 acacccaaagttgaaagcaa agagaaagtg atagtgaaaa aagacaagcc aggaaaggta 1500 gaaagtaagccatcggtgac ggaaaaggag gtgcccagca aagaggagca gtcgcccgtc 1560 aaagctgaggtggctgagaa ggcggccacg gagagcaaac ccaaagtcac caaagacaaa 1620 gtggtaaaaaaggaaataaa gacaaaaccc gaagaaaaga aagaggagaa gcccaagaag 1680 gaagtggctaaaaaggaaga caaaactccc ctcaagaaag acgagaagcc caaaaaggaa 1740 gaggcgaagaaggagatcaa gaaagaaatc aaaaaggaag agaaaaagga gctgaagaaa 1800 gaggtgaagaaggaaacgcc cctgaaggac gccaagaagg aggtgaagaa agacgagaag 1860 aaagaagttaaaaaggaaga gaaggaaccc aaaaaggaga ttaagaagat ctccaaggac 1920 ataaagaaatccactcctct gtcagacaca aagaaaccgg ctgcattgaa accaaaagta 1980 gcaaagaaagaagagcccac caagaaggag cctattgctg ctgggaaact caaggacaag 2040 gggaaggtcaaagtcattaa aaaggaaggc aagaccacag aggccgctgc cacagctgtt 2100 ggcactgctgccgtggctgc agcagccgga gtagcggcca gcggtcctgc caaagaactt 2160 gaagctgagcggtccctcat gtcgtcccct gaggatctaa ccaaggactt tgaggagcta 2220 aaggctgaggagatcgatgt agcgaaggac atcaagcctc agctggagct cattgaagat 2280 gaagagaaactgaaggaaac cgagccggga gaagcctacg tcattcagaa agagacggaa 2340 gtcagcaaaggttctgctga gtcacctgat gaagggatca ccaccactga gggggaaggg 2400 gagtgcgagcaaacccccga ggagctggag ccagttgaga agcagggcgt ggatgacatc 2460 gagaagttcgaggatgaagg cgctggtttt gaagaatcct cagaggccgg agactacgaa 2520 gagaaggcagaaactgagga ggccgaggag ccggaagaag acggggaaga caatgtgagc 2580 gggagcgcctcgaagcacag ccccacagaa gacgaagaaa tcgctaaggc tgaggcggac 2640 gtacacatcaaggagaagag ggagtctgtg gccagcggcg atgaccgggc cgaagaagac 2700 atggatgaagcgcttgagaa aggagaagct gaacagtctg aggaggaggg tgaggaggag 2760 gaggacaaagcagaggacgc cagagaggaa gaccatgagc ccgacaaaac tgaggctgaa 2820 gattatgtgatggctgtggt tgacaaggcc gcggaggccg gagtcaccga ggatcagtat 2880 gatttcctggggacaccggc caagcaacct ggagtccagt ctcctagccg agaacccgcg 2940 tcttcaattcatgatgagac cctacccgga ggctccgaga gcgaggccac tgcttcagat 3000 gaggagaatcgagaagacca gcctgaggaa ttcactgcta cctccggata tactcagtcc 3060 accatcgagatatctagtga gccgactcca atggatgaga tgtccactcc tcgagatgtg 3120 atgaccgacgagaccaacaa tgaggagaca gagtccccgt ctcaggagtt cgtgaacatt 3180 accaaatacgagtcttcgct gtactctcag gagtactcca aacctgtggt tgcatcattc 3240 aatggattgtcagacgggtc aaagacagac gccactgacg gtagggatta caacgcttcc 3300 gcctccaccatatcaccacc ttcgtccatg gaagaagaca aattcagcaa gtctgctctt 3360 cgtgacgcttaccgcccaga agagacggac gtgaaaaccg gtgccgagtt ggacatcaaa 3420 gatgtttcggatgagagact tagcccagcc aagagtccat ccctgagtcc ttctccacca 3480 tcacccatagagaagactcc cctgggtgaa cgtagcgtga atttctctct gacacccaac 3540 gagatcaaagcctctgcaga gggagaggca acagcagtag tgtcccccgg agtgacccaa 3600 gcagtagttgaagaacactg tgccagtcct gaggagaaga ccttggaggt agtgtcaccg 3660 tctcagtctgtgacaggcag tgcgggccac acaccttact accaatctcc caccgacgaa 3720 aagtccagtcacctacctac agaagtcact gagaacgcgc aggcagtccc ggtgagcttt 3780 gaattcactgaggccaaaga tgagaacgag aggtcgtcca tcagccccat ggatgaacct 3840 gtgcctgactcagagtctcc tatcgagaaa gttctgtctc cgttacgcag ccctcccctt 3900 attggatccgagtccgcata tgaagacttc ctgagtgcgg atgacaaggc tcttggcaga 3960 cgttcagaaagcccctttga agggaagaat ggaaagcaag gcttctcaga caaagaaagc 4020 ccagtttctgacctgacttc cgatctttac caagacaagc aggaagagaa aagggcgggc 4080 ttcataccgataaaggaaga ctttagtcca gaaaagaaag ccagcgatgc tgaaatcatg 4140 agttctcaatcagctctggc tttggatgaa aggaaactgg gaggagatgg atctccaacg 4200 caagtagatgtcagtcagtt tggctctttc aaagaagaca ccaagatgtc catttcggaa 4260 ggcaccgtttcagacaagtc cgccacgcct gtggatgagg gcgcggaaga cacctattca 4320 cacatggaaggtgtggcctc agtgtcaacg gcctctgtgg ctaccagctc gtttccagag 4380 ccaaccacagatgacgtgtc tccttctctc cacgctgaag tgggctctcc acattccaca 4440 gaggtggatgactccctgtc ggtgtcggtg gtgcaaacac caactacttt ccaggaaaca 4500 gaaatgtctccgtctaaaga agagtgccca agaccaatgt cgatttctcc tcctgacttc 4560 tcccctaagacagccaaatc caggacacca gttcaagatc accgatccga acagtcttca 4620 atgtctattgaattcggtca ggaatccccc gagcattctc ttgctatgga ctttagtcgg 4680 cagtctccagaccaccctac tgtgggtgct ggtatgcttc acatcaccga aaatgggcca 4740 actgaggtggactacagtcc ctccgatatc caggactcta gtttgtcaca taagattcct 4800 cctacagaagagccatccta cacccaggat aatgatctgt ccgagctcat ctctgtgtct 4860 caggtggaggcttccccatc cacctcttct gctcacactc cttctcagat agcctctcct 4920 cttcaggaagacactctctc tgatgtcgtt cctcccagag atatgtcctt atatgcctcg 4980 cttgcgtctgagaaagtgca gagcctggaa ggagagaaac tctctccaaa atccgatatt 5040 tctccgctcacccctcgaga gtcctcacct acatattcac ctggcttttc agattctacc 5100 tctggagctaaagagagtac agcggcttac caaacctcct cttccccacc aatagatgca 5160 gcagccgcagagccctacgg cttccgctcc tcaatgttat ttgatacaat gcagcatcac 5220 ctggccttgagtagagattt gaccacatct agtgtggaga aggacaatgg agggaagaca 5280 cccggtgactttaactatgc ctatcaaaag cccgagagca ccaccgaatc cccagatgaa 5340 gaagattatgactatgaatc tcacgagaaa accatccagg cccacgatgt gggtggttac 5400 tactatgagaagacagagag aaccataaaa tccccatgtg acagtggata ctcctatgag 5460 accattgagaagaccaccaa gaccccagaa gatggtggct actcctgtga aattaccgag 5520 aaaaccactcggacccctga agagggcggg tactcgtatg agatcagcga gaagacaaca 5580 cgaacccctgaagtaagtgg ctacacctat gagaagaccg agaggtccag aaggctcctc 5640 gatgacattagcaatggcta cgatgacact gaggatggtg gccacacact tggcgactgt 5700 agctattcctacgaaaccac tgagaaaatt accagctttc ctgaatctga aagctattcc 5760 tatgagacaactacaaaaac aacacggagt ccagacacct ctgcatactg ttacgagacc 5820 atggagaagatcaccaagac cccacaggca tccacatact cctatgagac ctcagaccga 5880 tgctacactccagaaaggaa gtccccctcg gaggcacgcc aggatgttga cttgtgtctg 5940 gtgtcctcctgtgaattcaa gcatcccaag accgagctct caccttcctt cattaatcca 6000 aaccctctcgagtggtttgc tggggaagag cccactgaag aatctgagag gcctctcact 6060 cagtctggaggagccccccc accttcagga ggaaaacaac agggcagaca atgcgatgaa 6120 actccacccacctcagtcag tgagtcagct ccatcccaga cggactctga tgttccccca 6180 gagacagaagagtgcccctc catcacagct gatgccaacc ttgactctga agatgagtca 6240 gaaaccatccccacagacaa aacggttacg tacaaacaca tggacccgcc tccagccccc 6300 atgcaagaccgaagcccttc tcctcgccac cctgatgtgt ccatggtgga tccagaggcc 6360 ttggctattgagcagaacct aggcaaggct ctgaaaaagg atctgaagga gaaggccaag 6420 accaagaaaccaggcacaaa gaccaagtcc tcttcacctg tcaaaaaggg tgatgggaag 6480 tccaagccttcagcagcttc ccccaaacca ggagccttga aggaatcctc tgacaaggtg 6540 tccagagtggcttctcccaa gaagaaagag tctgtggaga aagctatgaa gaccaccacc 6600 actcctgaggtcaaagccac acgaggggaa gagaaggaca aggaaactaa gaatgcagcc 6660 aatgcttctgcatccaagtc agtgaagact gcaacagcag gaccaggaac cactaagacg 6720 gccaagtcgtccaccgtgcc tcccggcctc cctgtgtatt tggacctctg ctatattccc 6780 aaccacagcaacagtaagaa tgtcgatgtt gagtttttca agagagtgag gtcatcttac 6840 tacgtggtgagtgggaacga ccctgccgcg gaggagccca gccgggctgt cctggatgcc 6900 ttgttggaagggaaggctca gtggggaagc aacatgcagg tgactctgat cccaacacat 6960 gactctgaggtgatgaggga gtggtaccag gagacccacg agaagcagca agacctcaac 7020 atcatggtcctagcaagcag tagtacagtg gtcatgcaag acgagtcctt ccctgcatgc 7080 aagatagaactgtag 7095 68 379 PRT Rattus norvegicus cdc 37 homolog 68 Met Val AspTyr Ser Val Trp Asp His Ile Glu Val Ser Asp Asp Glu 1 5 10 15 Asp GluThr His Pro Asn Ile Asp Thr Ala Ser Leu Phe Arg Trp Arg 20 25 30 His GlnAla Arg Val Glu Arg Met Glu Gln Phe Gln Lys Glu Lys Glu 35 40 45 Glu LeuAsp Arg Gly Cys Arg Glu Cys Lys Arg Lys Val Ala Glu Phe 50 55 60 Gln ArgLys Leu Lys Glu Leu Glu Val Ala Glu Gly Gly Gly Gln Val 65 70 75 80 GluLeu Glu Arg Leu Arg Ala Glu Ala Gln Gln Leu Arg Lys Glu Glu 85 90 95 ArgThr Gly Ser Arg Ser Trp Arg Thr Cys Gly Lys Lys Glu Lys Asn 100 105 110Met Pro Trp Asn Val Asp Thr Leu Ser Lys Asp Gly Phe Ser Lys Ser 115 120125 Met Val Asn Thr Lys Pro Glu Lys Ala Glu Glu Asp Ser Glu Glu Ala 130135 140 Arg Glu Gln Lys His Lys Thr Phe Val Glu Lys Tyr Glu Lys Gln Ile145 150 155 160 Lys His Phe Gly Met Leu His Arg Trp Asp Asp Ser Gln LysTyr Leu 165 170 175 Ser Asp Asn Val His Leu Val Cys Glu Glu Thr Ala AsnTyr Leu Val 180 185 190 Ile Trp Cys Ile Asp Leu Glu Val Glu Glu Lys CysAla Leu Met Glu 195 200 205 Gln Val Ala His Gln Thr Met Val Met Gln PheIle Leu Glu Leu Ala 210 215 220 Lys Ser Leu Lys Val Asp Pro Arg Ala CysPhe Arg Gln Phe Phe Thr 225 230 235 240 Lys Ile Lys Thr Ala Asp Gln GlnTyr Met Glu Gly Phe Lys Tyr Glu 245 250 255 Leu Glu Ala Phe Lys Glu ArgVal Arg Gly Arg Ala Lys Leu Arg Ile 260 265 270 Glu Lys Ala Met Lys GluTyr Glu Glu Glu Glu Arg Lys Lys Arg Leu 275 280 285 Gly Pro Gly Gly LeuAsp Pro Val Glu Val Tyr Glu Ser Leu Pro Glu 290 295 300 Glu Leu Gln LysCys Phe Asp Val Lys Asp Val Gln Met Leu Gln Asp 305 310 315 320 Ala IleSer Lys Met Asp Pro Thr Asp Ala Lys Tyr His Met Gln Arg 325 330 335 CysIle Asp Ser Gly Leu Trp Val Pro Asn Ser Lys Ser Gly Glu Ala 340 345 350Lys Glu Gly Glu Glu Ala Gly Pro Gly Asp Pro Leu Leu Glu Ala Val 355 360365 Pro Lys Ala Gly Phe Glu Lys Asp Ile Ser Ala 370 375 69 1604 DNARattus norvegicus cdc 37 homolog 69 cgagcggact ctctagccct tgtgggtccctgttcagcgc ccggccgtgt ggagacccgg 60 gagccagtcc gggcctccga ggcgaagatggtggactaca gcgtttggga tcacatcgag 120 gtgtcggacg atgaggacga gacgcaccccaacatagaca cggccagcct gttccgctgg 180 cggcaccagg cccgggtgga acgcatggaacagtttcaga aggagaaaga agaactggac 240 cggggctgcc gggaatgcaa gcgcaaggtagccgagttcc agcgcaagct gaaggaactg 300 gaagtggctg agggtggtgg ccaggtggagcttgagcggc ttcgagcgga ggcacagcag 360 ctgcgcaagg aggagcgaac tgggagcagaagctggagga catgcggcaa aaaggagaag 420 aacatgccct ggaatgtgga cacactcagcaaagatggct tcagcaagag catggtcaat 480 accaagcctg agaaggcaga ggaagactcagaggaggcaa gggagcagaa acacaagacc 540 ttcgttgaaa aatacgagaa acagatcaaacatttcggca tgctccaccg ctgggatgac 600 agccagaagt acctgtcaga caatgtccacctggtgtgcg aggagacagc caactacctg 660 gttatctggt gcattgacct ggaagtcgaggagaagtgtg ccctgatgga gcaggtggct 720 caccagacca tggtcatgca gttcatcctggagttggcca agagtctgaa ggtcgacccc 780 cgggcctgct tccggcagtt tttcaccaagatcaagactg ctgaccagca gtacatggag 840 ggcttcaagt atgaactgga ggcctttaaggagcgtgtgc ggggccgcgc caagctgcga 900 atagagaagg ccatgaagga gtacgaggaggaggagcgca agaagaggct aggccctggt 960 ggcctggacc ctgtggaggt ctacgaatctctgcctgagg agctgcagaa gtgctttgat 1020 gtgaaggatg tacagatgct gcaagacgccatcagcaaga tggatcctac tgatgccaag 1080 tatcacatgc agcgttgcat cgattctggcctctgggtcc ccaactccaa gtctggtgag 1140 gccaaggagg gggaggaggc gggccccggggacccattgc tggaagccgt ccccaaagcg 1200 ggcttcgaga aagacatcag tgcgtgacccttctccagaa aaggaaaccc agccaccatg 1260 tccctagctc ccgacttctt cacttctgctgctcctcacg tcctggccca tggcctgccc 1320 catgtcccct ctcctccggc ctgtccccagcactcatcag attctctgct tcgggttgag 1380 gggctctgct gtctgcagcc cccccacccccatcagcgtt atgccaaagt cctgggggtc 1440 tggggagggg cagcagctgc caggttggtccaccgggttg gggatacaca tgtccccatc 1500 tgggcagggt ctgtccctat gggtctatggtcactgtttc tcttctgtct agctttctgt 1560 cctgcaactg gcaacaatgt ccaataaacagttccaggtg ctgg 1604 70 216 PRT Homo sapiens Rab5c-like protein 70 MetAla Gly Arg Gly Gly Ala Arg Arg Pro Asn Gly Pro Ala Ala Gly 1 5 10 15Asn Lys Ile Cys Gln Phe Lys Leu Val Leu Leu Gly Glu Ser Ala Val 20 25 30Gly Lys Ser Ser Leu Val Leu Arg Phe Val Lys Gly Gln Phe His Glu 35 40 45Tyr Gln Glu Ser Thr Ile Gly Ala Ala Phe Leu Thr Gln Thr Val Cys 50 55 60Leu Asp Asp Thr Thr Val Lys Phe Glu Ile Trp Asp Thr Ala Gly Gln 65 70 7580 Glu Arg Tyr His Ser Leu Ala Pro Met Tyr Tyr Arg Gly Ala Gln Ala 85 9095 Ala Ile Val Val Tyr Asp Ile Thr Asn Thr Asp Thr Phe Ala Arg Ala 100105 110 Lys Asn Trp Val Lys Glu Leu Gln Arg Gln Ala Ser Pro Asn Ile Val115 120 125 Ile Ala Leu Ala Gly Asn Lys Ala Asp Leu Ala Ser Lys Arg AlaVal 130 135 140 Glu Phe Gln Glu Ala Gln Ala Tyr Ala Asp Asp Asn Ser LeuLeu Phe 145 150 155 160 Met Glu Thr Ser Ala Lys Thr Ala Met Asn Val AsnGlu Ile Phe Met 165 170 175 Ala Ile Ala Lys Lys Leu Pro Lys Asn Glu ProGln Asn Ala Thr Gly 180 185 190 Ala Pro Gly Arg Asn Arg Gly Val Asp LeuGln Glu Asn Asn Pro Ala 195 200 205 Ser Arg Ser Gln Cys Cys Ser Asn 210215 71 1529 DNA Homo sapiens Rab5c-like protein 71 cttacactaa gtgcctctttgcatagcacc agtccccacc cgcacgctct ctggaccact 60 acagctggac gggcaatggcgggtcgggga ggcgcacgac gacccaatgg accagctgct 120 gggaacaaga tctgtcaatttaagctggtt ctgctggggg agtctgcggt aggcaaatcc 180 agcctcgtcc tccgctttgtcaagggacag tttcacgagt accaggagag cacaattgga 240 gcggccttcc tcacacagactgtctgcctg gatgacacaa cagtcaagtt tgagatctgg 300 gacacagctg gacaggagcggtatcacagc ctggccccca tgtactatcg gggggcccag 360 gctgccatcg tggtctatgacatcaccaac acagatacat ttgcacgggc caagaactgg 420 gtgaaggagc tacagaggcaggccagcccc aacatcgtca ttgcactcgc gggtaacaag 480 gcagacctgg ccagcaagagagccgtggaa ttccaggaag cacaagccta tgcagacgac 540 aacagtttgc tgttcatggagacatcagca aagactgcaa tgaacgtgaa cgaaatcttc 600 atggcaatag ctaagaagcttcccaagaac gagccccaga atgcaactgg tgctccaggc 660 cgaaaccgag gtgtggacctccaggagaac aacccagcca gccggagcca gtgctgcagc 720 aactgagccc cccttgcctgcccgctgccc ccgcctcctc cgcctgaatg acccgactgg 780 aatccactct aaccaatcgcacttaacgac tcgggccacc actggggggg cagggggagg 840 ggtccaccat gatttctccatataattttg atcataggcc ggagtgagtc attccacctg 900 cacctttctg tacaaatactaattcaattt taagtcttaa gtcacttttt taatatatat 960 gatcttctgc tcttcccacttcctcccctt tctactgctc tcccattttc ccttgctggg 1020 agtagccaca tgctcttgccccccaaccct tgtatatggg gacagtgggg tcagtgcagc 1080 taccctttct ttccctctgcggaacagcgg acccagcaag agcatccaca tcctcacttt 1140 gttcggagtg gtctttggtttgggcggtgg ggcagacctt gggaaggggc ttaggaaggg 1200 agaggcagct cttccttcagctggctctca tcaggctgca gccccctccc cgctcccacc 1260 tccctgctgg gaaaccacagcattatcaca gcattattgt gacagccacg aacccattgc 1320 ccacaacccc tccaccctcggtcaccccaa cctctggctc tgagccctgt tctgaccaaa 1380 tcatgatgat gagtatttgggggtgggtgg gtaagggggg gagtgggagg ggacggaacc 1440 aactttttct gtattttgtattgtatgttt tcttcaacat gtaaccaatc agtatcttgt 1500 caatatagtc agccgatcgatcgacctca 1529 72 731 PRT Mus musculus Gelsolin 72 Met Val Val Glu HisPro Glu Phe Leu Lys Ala Gly Lys Glu Pro Gly 1 5 10 15 Leu Gln Ile TrpArg Val Glu Lys Phe Asp Leu Val Pro Val Pro Pro 20 25 30 Asn Leu Tyr GlyAsp Phe Phe Thr Gly Asp Ala Tyr Val Ile Leu Lys 35 40 45 Thr Val Gln LeuArg Asn Gly Asn Leu Gln Tyr Asp Leu His Tyr Trp 50 55 60 Leu Gly Asn GluCys Ser Gln Asp Glu Ser Gly Ala Ala Ala Ile Phe 65 70 75 80 Thr Val GlnLeu Asp Asp Tyr Leu Asn Gly Arg Ala Val Gln His Arg 85 90 95 Glu Val GlnGly Phe Glu Ser Ser Thr Phe Ser Gly Tyr Phe Lys Ser 100 105 110 Gly LeuLys Tyr Lys Lys Gly Gly Val Ala Ser Gly Phe Lys His Val 115 120 125 ValPro Asn Glu Val Val Val Gln Arg Leu Phe Gln Val Lys Gly Arg 130 135 140Arg Val Val Arg Ala Thr Glu Val Pro Val Ser Trp Asp Ser Phe Asn 145 150155 160 Asn Gly Asp Cys Phe Ile Leu Asp Leu Gly Asn Asn Ile Tyr Gln Trp165 170 175 Cys Gly Ser Gly Ser Asn Lys Phe Glu Arg Leu Lys Ala Thr GlnVal 180 185 190 Ser Lys Gly Ile Arg Asp Asn Glu Arg Ser Gly Arg Ala GlnVal His 195 200 205 Val Ser Glu Glu Glu Thr Glu Pro Glu Ala Met Leu GlnVal Leu Gly 210 215 220 Pro Lys Pro Ala Leu Pro Glu Gly Thr Glu Asp ThrAla Lys Glu Asp 225 230 235 240 Ala Ala Asn Arg Lys Leu Ala Lys Leu TyrLys Val Ser Asn Gly Ala 245 250 255 Gly Ser Met Ser Val Ser Leu Val AlaAsp Glu Asn Pro Phe Ala Gln 260 265 270 Gly Pro Leu Arg Ser Glu Asp CysPhe Ile Leu Asp His Gly Arg Asp 275 280 285 Gly Lys Ile Phe Val Trp LysGly Lys Gln Ala Asn Met Glu Glu Arg 290 295 300 Lys Ala Ala Leu Lys ThrAla Ser Asp Phe Ile Ser Lys Met Gln Tyr 305 310 315 320 Pro Arg Gln ThrGln Val Ser Val Leu Pro Glu Gly Gly Glu Thr Pro 325 330 335 Leu Phe LysGln Phe Phe Lys Asn Trp Arg Asp Pro Asp Gln Thr Asp 340 345 350 Gly ProGly Leu Gly Tyr Leu Ser Ser His Ile Ala Asn Val Glu Arg 355 360 365 ValPro Phe Asp Ala Gly Thr Leu His Thr Ser Thr Ala Met Ala Ala 370 375 380Gln His Gly Met Asp Asp Asp Gly Thr Gly Gln Lys Gln Ile Trp Arg 385 390395 400 Ile Glu Gly Ser Asn Lys Val Pro Val Asp Pro Ala Thr Tyr Gly Gln405 410 415 Phe Tyr Gly Gly Asp Ser Tyr Ile Ile Leu Tyr Asn Tyr Arg HisGly 420 425 430 Gly Arg Gln Gly Gln Ile Ile Tyr Asn Trp Gln Gly Ala GlnSer Thr 435 440 445 Gln Asp Glu Val Ala Ala Ser Ala Ile Leu Thr Ala GlnLeu Asp Glu 450 455 460 Glu Leu Gly Gly Thr Pro Val Gln Ser Arg Val ValGln Gly Lys Glu 465 470 475 480 Pro Ala His Leu Met Ser Leu Phe Gly GlyLys Pro Met Ile Ile Tyr 485 490 495 Lys Gly Gly Thr Ser Arg Asp Gly GlyGln Thr Ala Pro Ala Ser Ile 500 505 510 Arg Leu Phe Gln Val Arg Ala SerSer Ser Gly Ala Thr Arg Ala Val 515 520 525 Glu Val Met Pro Lys Ser GlyAla Leu Asn Ser Asn Asp Ala Phe Val 530 535 540 Leu Lys Thr Pro Ser AlaAla Tyr Leu Trp Val Gly Ala Gly Ala Ser 545 550 555 560 Glu Ala Glu LysThr Ala Ala Gln Glu Leu Leu Lys Val Leu Arg Ser 565 570 575 Gln His ValGln Val Glu Glu Gly Ser Glu Pro Asp Gly Phe Trp Glu 580 585 590 Ala LeuGly Gly Lys Thr Ser Tyr Arg Thr Ser Pro Arg Leu Lys Asp 595 600 605 LysLys Met Asp Ala His Pro Pro Arg Leu Phe Ala Cys Ser Asn Arg 610 615 620Ile Gly Arg Phe Val Ile Glu Glu Val Pro Gly Glu Leu Met Gln Glu 625 630635 640 Asp Leu Ala Thr Asp Asp Val Met Leu Leu Asp Thr Trp Asp Gln Val645 650 655 Phe Val Trp Val Gly Lys Asp Ser Gln Glu Glu Glu Lys Thr GluAla 660 665 670 Leu Thr Ser Ala Lys Arg Tyr Ile Glu Thr Asp Pro Ala AsnArg Asp 675 680 685 Arg Arg Thr Pro Ile Thr Val Val Arg Gln Gly Phe GluPro Pro Ser 690 695 700 Phe Val Gly Trp Phe Leu Gly Trp Asp Asn Asn TyrTrp Ser Val Asp 705 710 715 720 Pro Leu Asp Arg Ala Leu Ala Glu Leu AlaAla 725 730 73 2447 DNA Mus musculus Gelsolin 73 tgagcgcggc ccagcactatggtggtggag caccccgaat tcctgaaggc agggaaggag 60 cctggcctgc agatctggcgtgtggagaag tttgacctgg tgcctgtgcc ccccaacctc 120 tatggagact tcttcacgggtgatgcctat gtcatcctga agactgtgca gctgaggaat 180 gggaatctgc agtatgacctccactattgg ctgggcaatg aatgcagcca ggatgagagc 240 ggggctgctg ccatctttactgtgcaactg gatgactacc tgaacggccg ggctgtgcag 300 caccgtgagg ttcagggctttgagtcgtcc accttctccg gctacttcaa gtctggactt 360 aagtacaaga aaggaggtgtggcatctgga ttcaaacacg tggtacccaa tgaggtggtg 420 gtccagaggc tcttccaggtcaaaggacgc cgtgtagtcc gtgctactga ggtacctgtg 480 tcctgggaca gtttcaacaatggcgactgc ttcattctgg acctgggaaa caatatctat 540 cagtggtgtg gctctggcagcaacaaattt gaaaggctga aggccacaca ggtgtccaag 600 ggcatccggg acaacgagaggagtggccgt gctcaagtac acgtgtctga agaggagact 660 gagcccgagg cgatgctgcaggtgctgggc cccaagccgg ctctgcctga aggtaccgag 720 gacacagcca aggaagatgcagccaaccgc aagctggcca agctctacaa ggtctccaac 780 ggtgcaggta gcatgtcagtctccctagtg gctgatgaga accccttcgc ccaggggccc 840 ctgagatctg aggactgcttcatcctggac catggcagag atgggaaaat ctttgtttgg 900 aaaggcaagc aggccaacatggaggagcgg aaggctgccc tcaaaacagc ctctgacttc 960 atctccaaga tgcagtaccccaggcagacc caggtttcag ttctcccaga gggcggtgag 1020 acccctctct ttaagcagttcttcaagaac tggcgggacc cagaccagac agatggcccc 1080 ggcctgggct acctctccagccacattgcc aacgtggagc gcgtaccttt cgatgccggc 1140 acgctgcaca cctccaccgccatggccgct cagcacggca tggatgatga tggaactggc 1200 cagaaacaga tctggagaattgaaggttcc aacaaggtgc cagtggaccc tgccacatac 1260 ggacagttct atggaggcgacagctacatc attctgtaca actaccgcca cggtggccgc 1320 cagggacaga tcatctacaactggcagggt gctcagtcta cccaggatga ggttgctgct 1380 tctgccatcc tgactgcccagctggatgag gagctgggag gaactcctgt ccagagccga 1440 gtggtccaag gcaaagagcctgcacacctc atgagcttgt ttggcgggaa gcccatgatc 1500 atctacaagg gtggcacctcccgtgatggt gggcagacag ctcctgccag tatccgcctc 1560 ttccaagtgc gtgccagcagctctggagcc accagggctg tggaggtgat gcctaagtct 1620 ggtgctctga actccaacgatgcctttgtg ctgaaaaccc cctccgctgc ctacctgtgg 1680 gtgggcgcag gagccagtgaggcagagaag acggcggccc aggagcttct gaaggtcctt 1740 cggtcccagc atgtgcaggtggaagaaggc agtgagccag atggcttctg ggaggctctg 1800 ggcgggaaga cgtcctaccgcacatccccc aggcttaagg acaagaagat ggatgcccat 1860 cctcctcgac tctttgcctgctccaacagg atcggacgct ttgtgatcga agaggttcct 1920 ggcgagctta tgcaggaagacctggctact gatgacgtca tgctcctgga cacctgggac 1980 caggtctttg tctgggttggaaaagactcc caggaagaag aaaagacgga agccttgact 2040 tctgctaagc ggtacatcgagacagatcca gcaaatcggg acaggcggac ccccatcaca 2100 gtcgttaggc agggctttgagcctccttcc ttcgtgggct ggttcctcgg ctgggacaac 2160 aactactggt cggtggatcctttggaccgg gccttggctg agctggctgc ctgagtaagg 2220 accaagccat caatgtcaccaatcagtgcc tttgagggtt gtccatctcc caaagacatc 2280 atatggcaag caggaaaactatgatgtgtg cgcgcgtgtt tttgtttttg ttttttacgg 2340 tagccaaaac aagcccttgtggaaactcag ggtctttaca gaattgcttc aaatgtctgt 2400 actttggaaa tgaaagccaataaaagcttt ttgaagtgaa aaaaaaa 2447 74 236 PRT Rattus norvegicus Cd81antigen 74 Met Gly Val Glu Gly Cys Thr Lys Cys Ile Lys Tyr Leu Leu PheVal 1 5 10 15 Phe Asn Phe Val Phe Trp Leu Ala Gly Gly Val Ile Leu GlyVal Ala 20 25 30 Leu Trp Leu Arg His Asp Pro Gln Thr Thr Thr Leu Leu TyrLeu Glu 35 40 45 Leu Gly Asp Lys Pro Ala Pro Ser Thr Phe Tyr Val Gly IleTyr Ile 50 55 60 Leu Ile Ala Val Gly Ala Val Met Met Phe Val Gly Phe LeuGly Cys 65 70 75 80 Tyr Gly Ala Ile Gln Glu Ser Gln Cys Leu Leu Gly ThrPhe Phe Thr 85 90 95 Cys Leu Val Ile Leu Phe Ala Cys Glu Val Ala Ala GlyIle Trp Gly 100 105 110 Phe Val Asn Lys Asp Gln Ile Ala Lys Asp Val LysGln Phe Tyr Asp 115 120 125 Gln Ala Leu Gln Gln Ala Val Met Asp Asp AspAla Asn Asn Ala Lys 130 135 140 Ala Val Val Lys Thr Phe His Glu Thr LeuAsn Cys Cys Gly Ser Asn 145 150 155 160 Thr Leu Thr Thr Leu Thr Thr AlaVal Leu Arg Asn Ser Leu Cys Pro 165 170 175 Ser Ser Ser Asn Ser Phe ThrGln Leu Leu Lys Glu Asp Cys His Gln 180 185 190 Lys Ile Asp Glu Leu PheSer Gly Lys Leu Tyr Leu Ile Gly Ile Ala 195 200 205 Ala Ile Val Val AlaVal Ile Met Ile Phe Glu Met Ile Leu Ser Met 210 215 220 Val Leu Cys CysGly Ile Arg Asn Ser Ser Val Tyr 225 230 235 75 1303 DNA Rattusnorvegicus Cd81 antigen 75 gtggcggtct gagcgagcgc gcccttgctt caaagcggtagcgactctag cgcctccggc 60 taggctccaa cccttctcta ccctacgtct caatagccacaccgcagtct ccggcctcaa 120 gcgacccagg ctatcttcca gtcccgggac tccgggtactgcggtcccca gaaccgcccg 180 tccagggacc aacccaagtt ccgcaggccg cggcggccgccaatgggggt ggagggctgc 240 accaaatgca tcaaatacct gctcttcgtc ttcaatttcgtcttctggct ggctggaggt 300 gtgatcctag gtgtagctct gtggttgcgc catgatccacagaccaccac cttgctctac 360 ctggaactgg gagacaaacc agcacctagc accttctatgtgggcatcta cattctcatt 420 gctgtgggag ctgtgatgat gtttgtaggc ttcctggggtgctatggggc catccaggag 480 tcccagtgcc tgctggggac gttcttcact tgccttgtgatcctgtttgc ctgtgaggta 540 gctgcgggca tctggggctt tgtaaacaaa gaccagatcgccaaggatgt gaagcagttc 600 tacgaccagg cccttcagca ggctgtgatg gatgatgatgccaacaatgc caaggcagtg 660 gtgaagacct tccatgagac gctcaactgt tgtggctccaatacgctgac cacactgacc 720 accgccgtgc tgaggaacag cctgtgtccc tcaagcagcaactcattcac tcagcttctg 780 aaggaagact gccatcagaa aatcgatgag ctcttttctgggaagctgta cctcattgga 840 attgcagcca ttgtggtagc tgtcattatg atcttcgagatgattctgag catggtgctg 900 tgctgtggca tccggaacag ctccgtgtac tgaggcccttcgcttcgcac cagagggtcc 960 ctggaatgac cagagggcac cttggggggc atggcctgtgtatatactat ttctgtatta 1020 ctctgctaca cttagccttt ttacttttga gtcttttttgttctgaactt ttcctgtcac 1080 cttttggagc cgacaccaca cataggtggc gtatgtggggacgtagaggt ggagctggcc 1140 ctagctggca gggccctgta cttctgggac ccctggagagttctgcctgc tgagccaaac 1200 ctcctccata gctacttgtc cagaggccac atagccaagctagaaggcca tgtccaccca 1260 ctctgcccac tgtgggtcac atcgctccca tctttttaatccg 1303 76 81 PRT Rattus norvegicus MOBP-81 protein 76 Met Ser Gln LysVal Ala Lys Glu Gly Pro Arg Leu Ser Lys Asn Gln 1 5 10 15 Lys Phe SerGlu His Phe Ser Ile His Cys Cys Pro Pro Phe Thr Phe 20 25 30 Leu Asn SerLys Arg Glu Ile Val Asp Arg Lys Tyr Ser Ile Cys Lys 35 40 45 Ser Gly CysPhe Tyr Gln Lys Lys Glu Glu Asp Trp Ile Cys Cys Ala 50 55 60 Cys Gln LysThr Arg Leu Arg Arg Arg Ser Arg Ser Thr Pro Arg Lys 65 70 75 80 Lys 772405 DNA Rattus norvegicus MOBP-81 protein 77 ctttcacagc agccaatacctgcagggcaa caaagaatca aatgagagcg agacaagctg 60 ggaatgaagt cctggttgccagatgggagc ttgaaaacac agtaagatga gtcaaaaagt 120 ggccaaggag ggccccaggctctccaagaa ccagaagttc tcagagcact tcagcatcca 180 ctgctgccca cccttcaccttcctcaactc caagcgtgag atcgtggacc gcaagtacag 240 catctgcaag agcggttgcttttaccagaa gaaggaggag gactggatct gctgtgcctg 300 ccagaagacc agattgagaaggaggagcag gtcaacccca agaaaaaagt gaccgagaag 360 ggacttctcg tgaatggacagcctctgctt gtggatttac tgcttcaccg caacccatct 420 gccctcagac ttatctggcctcgagtatga cgcaggggtg gacacctgct gatgtaacaa 480 ctacagatgc cctcagtccccatggtgtgg gagccttagg gcagcctgcc tggagcactt 540 ggacgatgcc actccatcacctccctctat aaccgccgaa gaggcatctc tgtccacctt 600 ctgaccctcc atggttgttcagtctgaaga ttcctgtgtc ttcagggatg tgcaaaacaa 660 ggtcatggtg tgcgaccatccaaatggcaa atataaaaaa gaaattgtca agtgttggcg 720 gtgacgcaag aaaaatcctagaagccacag atggggattt atacactgtc gcaacaccgt 780 tgagatgtac tctggggacactatcaaggc aaagtatctt aaaacctcta atcaagcgac 840 tgtaatttta ggcatccatcctggggaaaa ctcacaccct tttagagaaa ggagaaatgc 900 ccaaatttgt ttaaaagatcgtggtttgta gttaagagag gtagaatgag attaaccaaa 960 atgtcggtcc acgggagagcaagagagtca atcttggtat gtccacaaag tcctaagagc 1020 agttaaaagc caatctgtcagacagaccca tgccgagcag gtaaatctag aaagcaaacc 1080 aaggactata aaagtcaagcaccaggcagt aaatccagaa taagagccta tgcatacata 1140 ttgacagctc acaatcctgtactggttatt gcttacataa ggagaagcca cagatctcat 1200 tctttcttct agaatgcaatagaatttaaa aatgacagca gaacaaaagg acactagaaa 1260 ttatcctctc agaaataaaagtataagaaa taatttctgg cttgaggaaa taacccaggc 1320 taaaagctac acaggcctggcttaaccata ccagcagggc atacctttgt taggagaggg 1380 tcaattcgaa gtttgtgaacagcctgggct gtttccgagg ctgcataaat cttaaacacc 1440 aaaaatgtgt gttttgaattaactttgggt ctggtctaca gagtccggct tgtgatggct 1500 cctgacacgg gagaaaaggggataagttaa tataaggctc tgggattttc ctttgaggaa 1560 caggcaggta ggaaaggacactagaatgtt ggggaatgtg gagggatgta aaacagacag 1620 tattatgatg gggggaactcaggaaggaac ggtaaggaca ccagaatggc ctcgtgctgg 1680 gagccagggt ggccaagcccaggcagatgc acaaagaccc caaaggccac cattgcctca 1740 caaccagacc cagcaaagttcagatcaagt gcataaatgg gggtcacgtc agtggaaact 1800 gtccggagca tggagcagggggtgaggaag acagtcagaa ggaagcaaga gatggcttag 1860 agtgttgcat ggcagcaagaagaagcagtc caaatatcat cccaaggcag ccatggcttc 1920 ctctcccgag gacactgtggccctgtggaa gtgccaaggc agaggcagag gaatttaacc 1980 gcacacaaac tacatgcagaaactaaggtg ctaaatgaac atctacgtga caacctttct 2040 gaggaccagg ttccagtgagcggcccaggg ccgttatctt cgcgatggta actgagctga 2100 gaagaatggg aaccggataggagcacacag tagcccactg gtgagacaaa tccacgggcg 2160 agcctcaacc aacccactaaggaaacttca cgccttttca tctagttttc attttggcaa 2220 agcaaagcca tcctgagtgcttgcttgtct ccgccctcca cgccaccccc gagacacaga 2280 gcatgcgcat taacccagagcgcgctaaca catgcacagc gttggacatt agcctattaa 2340 tgggaataac cagaaactactaaattttgt ttgttgtaga aataaaacct ggggatggat 2400 caaaa 2405 78 590 PRTHomo sapiens Syntaxin binding protein 2 78 Met Ala Pro Ser Gly Leu LysAla Val Val Gly Glu Lys Ile Leu Ser 1 5 10 15 Gly Val Ile Arg Ser ValLys Lys Asp Gly Glu Trp Lys Val Leu Ile 20 25 30 Met Asp His Pro Ser MetArg Ile Leu Ser Ser Cys Cys Lys Met Ser 35 40 45 Asp Ile Leu Ala Glu GlyIle Thr Ile Val Glu Asp Ile Asn Lys Arg 50 55 60 Arg Glu Pro Ile Pro SerLeu Glu Ala Ile Tyr Leu Leu Ser Pro Thr 65 70 75 80 Glu Lys Ala Leu IleLys Asp Phe Gln Gly Thr Pro Thr Phe Thr Tyr 85 90 95 Lys Ala Ala His IlePhe Phe Thr Asp Thr Cys Pro Glu Pro Leu Phe 100 105 110 Ser Glu Leu GlyArg Ser Arg Leu Ala Lys Val Val Lys Thr Leu Lys 115 120 125 Glu Ile HisLeu Ala Phe Leu Pro Tyr Glu Ala Gln Val Phe Ser Leu 130 135 140 Asp AlaPro His Ser Thr Tyr Asn Leu Tyr Cys Pro Phe Arg Ala Glu 145 150 155 160Glu Arg Thr Arg Gln Leu Glu Val Leu Ala Gln Gln Ile Ala Thr Leu 165 170175 Cys Ala Thr Leu Gln Glu Tyr Pro Ala Ile Arg Tyr Arg Lys Gly Pro 180185 190 Glu Asp Thr Ala Gln Leu Ala His Ala Val Leu Ala Lys Leu Asn Ala195 200 205 Phe Lys Ala Asp Thr Pro Ser Leu Gly Glu Gly Pro Glu Lys ThrArg 210 215 220 Ser Gln Leu Leu Ile Met Asp Arg Ala Ala Asp Pro Val SerPro Leu 225 230 235 240 Leu His Glu Leu Thr Phe Gln Ala Met Ala Tyr AspLeu Leu Asp Ile 245 250 255 Glu Gln Asp Thr Tyr Arg Tyr Glu Thr Thr GlyLeu Ser Glu Ala Arg 260 265 270 Glu Lys Ala Val Leu Leu Asp Glu Asp AspAsp Leu Trp Val Glu Leu 275 280 285 Arg His Met His Ile Ala Asp Val SerLys Lys Val Thr Glu Leu Leu 290 295 300 Arg Thr Phe Cys Glu Ser Lys ArgLeu Thr Thr Asp Lys Ala Asn Ile 305 310 315 320 Lys Asp Leu Ser Gln IleLeu Lys Lys Met Pro Gln Tyr Gln Lys Glu 325 330 335 Leu Asn Lys Tyr SerThr His Leu His Leu Ala Asp Asp Cys Met Lys 340 345 350 His Phe Lys GlySer Val Glu Lys Leu Cys Ser Val Glu Gln Asp Leu 355 360 365 Ala Met GlySer Asp Ala Glu Gly Glu Lys Ile Lys Asp Ser Met Lys 370 375 380 Leu IleVal Pro Val Leu Leu Asp Ala Ala Val Pro Ala Tyr Asp Lys 385 390 395 400Ile Arg Val Leu Leu Leu Tyr Ile Leu Leu Arg Asn Gly Val Ser Glu 405 410415 Glu Asn Leu Ala Lys Leu Ile Gln His Ala Asn Val Gln Ala His Ser 420425 430 Ser Leu Ile Arg Asn Leu Glu Gln Leu Gly Gly Thr Val Thr Asn Pro435 440 445 Gly Gly Ser Gly Thr Ser Ser Arg Leu Glu Pro Arg Glu Arg MetGlu 450 455 460 Pro Thr Tyr Gln Leu Ser Arg Trp Thr Pro Val Ile Lys AspVal Met 465 470 475 480 Glu Asp Ala Val Glu Asp Arg Leu Asp Arg Asn LeuTrp Pro Phe Val 485 490 495 Ser Asp Pro Ala Pro Thr Ala Ser Ser Gln AlaAla Val Ser Ala Arg 500 505 510 Phe Gly His Trp His Lys Asn Lys Ala GlyIle Glu Ala Arg Ala Gly 515 520 525 Pro Arg Leu Ile Val Tyr Val Met GlyGly Val Ala Met Ser Glu Met 530 535 540 Arg Ala Ala Tyr Glu Val Thr ArgAla Thr Glu Gly Lys Trp Glu Val 545 550 555 560 Leu Ile Gly Ser Ser HisIle Leu Thr Pro Thr Arg Phe Leu Asp Asp 565 570 575 Leu Lys Ala Leu AspLys Lys Leu Glu Asp Ile Ala Leu Pro 580 585 590 79 1888 DNA Homo sapiensSyntaxin binding protein 2 79 ggcacgaggg gcggcggcgc ccctcggggaagatggcgcc ctcggggctg aaggcggtgg 60 tgggggaaaa aattctgagc ggagttattcggagtgtcaa gaaggatggg gagtggaagg 120 tgcttatcat ggatcaccca agcatgcgcatcttgtcttc ctgctgcaaa atgtcagata 180 tcctggctga gggcatcacc attgttgaagacatcaacaa acggcgggaa cccattccca 240 gtctggaggc catttatttg ctgagccccacggagaaggc cctgatcaaa gacttccagg 300 ggaccccgac tttcacctac aaagcggcccatatcttctt caccgacacc tgccccgagc 360 ccctgttcag tgagctaggc cgctctcgtctggcaaaggt ggtgaagacg ttgaaggaga 420 ttcaccttgc cttcctcccc tacgaggcccaggtgttctc cctcgatgct ccccacagca 480 cctacaacct ctactgcccc ttccgggcagaggagcgcac gcggcagctc gaggtgctgg 540 cccagcagat tgccacgctg tgcgccaccctgcaggagta cccggccatc cgctaccgca 600 agggcccaga ggacacagcc cagttggcccacgccgtcct ggccaagctg aacgccttca 660 aggcagacac tcccagtctg ggcgagggcccagagaaaac ccgctcccag ctgctgataa 720 tggaccgggc agctgacccc gtgtccccactactgcatga gctcacgttc caggccatgg 780 cgtatgatct gctggacata gagcaggacacatacaggta tgagaccacc gggctgagcg 840 aggcgcggga gaaggccgtc ttgctggacgaggacgatga cttgtgggtg gagcttcgcc 900 acatgcatat cgcagatgtg tccaagaaggtcacggagct cctgaggacc ttctgtgaga 960 gcaagaggct gaccacggac aaggcgaacatcaaagacct atcccagatc ctgaaaaaga 1020 tgccgcagta ccagaaggag ctgaataagtattctacgca cctgcatcta gcagatgatt 1080 gtatgaagca cttcaagggc tcggtggagaagctgtgtag tgtggagcag gacctggcca 1140 tgggctccga cgcagagggg gagaagatcaaggactccat gaagctgatc gttccggtgc 1200 tgctggacgc ggcggtgccc gcctacgacaagatccgggt cctgctgctc tacatcctcc 1260 ttcggaatgg tgtgagtgag gagaacctggccaagctgat ccagcatgcc aatgtacagg 1320 cgcacagcag cctcatccgt aacctggagcagctgggagg cactgtcacc aaccccgggg 1380 gctcggggac ctccagccgg ctggagccgagagaacgcat ggagcccacc tatcagctgt 1440 cccgctggac cccggtcatc aaggatgtaatggaggacgc cgtggaggac cggctggaca 1500 ggaacctgtg gcccttcgta tccgaccccgcccccacggc cagctcccag gccgctgtca 1560 gtgcccgctt cggtcactgg cacaagaacaaggctggcat agaagcccgg gcgggccccc 1620 ggctcatcgt gtatgtcatg ggcggtgtggccatgtcaga gatgagggcc gcctacgagg 1680 tgaccagggc caccgagggc aagtgggaggtgctcattgg ctcctcacac atcctcaccc 1740 cgacccgctt cctggatgac ctgaaggcactggacaagaa gctggaggac attgccctgc 1800 cctgacccct ggccccgccc cctacccctccctttccaga gaaataaact cttcccgtcg 1860 ctctgccaaa aaaaaaaaaa aaaaaaaa1888 80 505 PRT Rattus norvegicus Alpha-internexin 80 Met Ser Phe GlySer Glu His Tyr Leu Cys Ser Ala Ser Ser Tyr Arg 1 5 10 15 Lys Val PheGly Asp Gly Ser Arg Leu Ser Ala Arg Leu Ser Gly Pro 20 25 30 Gly Ala SerGly Ser Phe Arg Ser Gln Ser Leu Ser Arg Ser Asn Val 35 40 45 Ala Ser ThrAla Ala Cys Ser Ser Ala Ser Ser Leu Gly Leu Gly Leu 50 55 60 Ala Tyr ArgArg Leu Pro Ala Ser Asp Gly Leu Asp Leu Ser Gln Ala 65 70 75 80 Ala AlaArg Thr Asn Glu Tyr Lys Ile Ile Arg Thr Asn Glu Lys Glu 85 90 95 Gln LeuGln Gly Leu Asn Asp Arg Phe Ala Val Phe Ile Glu Lys Val 100 105 110 HisGln Leu Glu Thr Gln Asn Arg Ala Leu Glu Ala Glu Leu Ala Ala 115 120 125Leu Arg Gln Arg His Ala Glu Pro Ser Arg Val Gly Glu Leu Phe Gln 130 135140 Arg Glu Leu Arg Glu Leu Arg Ala Gln Leu Glu Glu Ala Ser Ser Ala 145150 155 160 Arg Ala Gln Ala Leu Leu Glu Arg Asp Gly Leu Ala Glu Glu ValGln 165 170 175 Arg Leu Arg Ala Arg Cys Glu Glu Glu Ser Arg Gly Arg GluGly Ala 180 185 190 Glu Arg Ala Leu Lys Ala Gln Gln Arg Asp Val Asp GlyAla Thr Leu 195 200 205 Ala Arg Leu Asp Leu Glu Lys Lys Val Glu Ser LeuLeu Asp Glu Leu 210 215 220 Ala Phe Val Arg Gln Val His Asp Glu Glu ValAla Glu Leu Leu Ala 225 230 235 240 Thr Leu Gln Ala Ser Ser Gln Ala AlaAla Glu Val Asp Val Ala Val 245 250 255 Ala Lys Pro Asp Leu Thr Ser AlaLeu Arg Glu Ile Arg Ala Gln Tyr 260 265 270 Glu Ser Leu Ala Ala Lys AsnLeu Gln Ser Ala Glu Glu Trp Tyr Lys 275 280 285 Ser Lys Phe Ala Asn LeuAsn Glu Gln Ala Ala Arg Ser Thr Glu Ala 290 295 300 Ile Arg Ala Ser ArgGlu Glu Ile His Glu Tyr Arg Arg Gln Leu Gln 305 310 315 320 Ala Arg ThrIle Glu Ile Glu Gly Leu Arg Gly Ala Asn Glu Ser Leu 325 330 335 Glu ArgGln Ile Leu Glu Leu Glu Glu Arg His Ser Ala Glu Val Ala 340 345 350 GlyTyr Gln Asp Ser Ile Gly Gln Leu Glu Ser Asp Leu Arg Asn Thr 355 360 365Lys Ser Glu Met Ala Arg His Leu Arg Glu Tyr Gln Asp Leu Leu Asn 370 375380 Val Lys Met Ala Leu Asp Ile Glu Ile Ala Ala Tyr Arg Lys Leu Leu 385390 395 400 Glu Gly Glu Glu Thr Arg Phe Ser Thr Ser Gly Leu Ser Ile SerGly 405 410 415 Leu Asn Pro Leu Pro Asn Pro Ser Tyr Leu Leu Pro Pro ArgIle Leu 420 425 430 Ser Ser Thr Thr Ser Lys Val Ser Ser Ala Gly Leu SerLeu Lys Lys 435 440 445 Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Gly AlaSer Lys Glu Val 450 455 460 Thr Lys Lys Thr Ser Lys Val Gly Glu Ser PheGlu Glu Thr Leu Glu 465 470 475 480 Glu Thr Val Val Ser Thr Lys Lys ThrGlu Lys Ser Thr Ile Glu Glu 485 490 495 Ile Thr Thr Ser Ser Ser Gln LysMet 500 505 81 4593 DNA Rattus norvegicus Alpha-internexin 81 gaattctgaactacaaagca aagcgcttca catttccctg acttgtgcca taggctggaa 60 ggtaaactcagacaactgag ccatccattg ttcaggctct actctgtcct taccgggtgt 120 ggcgagggtggccagttctg tcagccgtgc tcctgcttca ctcctggaat tgaatcaaag 180 actaatccccacagaaaatg gttctcccat ctaattcaaa atggaatgtc aaacgcatac 240 agggccagacagatgaaaag acgaggtgga aagggtagct ctggagtgct gggagctgtg 300 aggaacaccgcacttaaaca aaggcactca cttccgccct ctgcagtcat cctaaacctt 360 ctactttcaagagtggcagg ggctgggcat taaaaggtca aaatcaatcc agtctctttt 420 tagatgaaacctcctgacta aaaattcatt aaaaaaaaac attttgaaat ctattgtttt 480 gttggtttgggtctttttgt ttaaaccaaa gcctgcagac tcctttctac cagcctgcgg 540 tcctgggatgcgcggttgat tggaggtcta gagatgaacc ctgctaattc tcaacgcttt 600 gctccttttccaggcctagc ctctttccct ccccgtaacc cttcgcaccc ttggaaataa 660 gcctcttgttcttcaccaat gtccggagca tctcacgctc tctgcgaatc ctcccgttta 720 ctgtggctgcggagctgaga gatgctctct gaagtggacc cactttctcc gaaaaggacc 780 aggagacacctaagtccaga agataaagag gagggtgctt ctccccctga aaagggtggg 840 gacagggcctcacccttggg aactctggga tgcagatgag aacagagaca cctttcagaa 900 aaacctactctccttggggg ataagcacag aaaaggaagg acaggctcta aagccatcaa 960 agagctgagtgctctgggct gggaagccaa ggttgttggg gcactccctc ccctccggtc 1020 attccctaggcagattcttg gggcacaggc gcagcggcca gggacggccc aggtcggtgg 1080 cgcgcagctgcggtggcact gcgcttcccc gcccccgcag gccccccttc ctctccccgc 1140 tagatctgaagatgaagctc cacccctagc gcgtcgcccc agccccgcgc cttaaaagcc 1200 ccgcacaccgccccgccgca cccagccttg ccgcaccttc gcgtcctcgc caggtccgcc 1260 gcagccgcgcacccggcccc gaccccggca ccatgagctt cggatcagag cactacttgt 1320 gctccgcctcctcctaccgc aaggtgttcg gggatggctc gcgcctgtct gcgcgcctgt 1380 ccgggccgggagcgtccggc agctttcgct cgcagtcgct gtcccgcagc aatgtggcct 1440 ccacggccgcttgctcctcg gcctcgtctc tcggcctggg cctggcctac cgccgcctgc 1500 cggcctccgacgggctggac ctgagccaag cagcggcgcg caccaacgag tacaagatca 1560 tccgcactaacgagaaggag cagctgcagg gcctcaacga ccgcttcgcg gtgttcatcg 1620 aaaaggtgcaccagctggag acgcagaacc gcgcgctcga ggccgagctg gccgcgctgc 1680 gccagcgccacgccgagccg tcgcgcgtcg gcgagctctt ccagcgcgag ctgcgcgagc 1740 tgcgcgcgcagctggaggag gcgagctccg cgcgcgcgca ggccctgctg gagcgcgacg 1800 ggctggccgaggaggtgcag cgactgcggg cgcgctgcga ggaggagagc cgcgggcgcg 1860 aaggcgcggagcgcgccctg aaggcgcagc agcgcgacgt ggacggcgcc actctggccc 1920 gcctggatctggagaagaag gtggagtcgc tgctggacga gctggccttc gtgcgccagg 1980 tgcacgacgaggaggtggcc gagctcctgg ccacgctgca ggcgtcttcg caagccgccg 2040 ccgaggtggacgtggctgtg gctaaaccag acctgacttc ggcgctgagg gagatccgcg 2100 cccagtatgagtccttggcc gctaagaacc tgcagtcggc cgaggagtgg tacaagtcca 2160 agttcgccaacctgaacgag caggctgcgc gcagcacgga agccatccga gccagccgag 2220 aggagatccacgagtaccgg cgccagctcc aggctcgtac cattgagata gagggcctgc 2280 gcggagccaatgaatccctg gagaggcaga tcttggaact ggaggagcgg cacagcgctg 2340 aggtggccggctaccaggat agcattgggc agctggagag tgacctgagg aacaccaaga 2400 gcgagatggcccgccacctt cgggaatacc aggacttgct caatgtcaaa atggctcttg 2460 acattgagatagcagcttac aggaagctgc tggaaggcga agagacacga tttagcacca 2520 gcgggttaagcatctcaggg ctgaatccac ttcccaatcc cagttatttg ctccctccta 2580 gaatccttagttctacaacc tccaaagtct catccgctgg gctgtccctg aaaaaggagg 2640 aggaggaggaggaggaagag gaagaagggg cttctaagga agtcactaag aaaacatcca 2700 aggtaggggaaagtttcgaa gaaacactgg aggaaacggt ggtatctacg aagaaaaccg 2760 aaaagtcaactatagaagaa atcaccacca gttccagcca aaaaatgtaa tgctgttgct 2820 taaaaaaaaaaaacaaaaaa aggcgatgcc taagagggaa tgcacttggc ttattcacag 2880 cttactcctgaaccacacct accaccgcaa tagaatgtct tcaaggcttc aatgtcatag 2940 tctgtaccgaatcctcgctc tctccagtag gaaggccact gtagctggga gcaaactgca 3000 gtgctggaggaggagcagga ggagctctct aagatcacag ctttcctacc tacagctcag 3060 gcctcatagtgacaggtgac ccaggtgcag aggaggctca aacgaaggtg ctaaatctgg 3120 tcatcgctcgctgtgagctg aaatcgaaac ctgtttctcc accgtacccc gccagtaccc 3180 agccatagaacctcactgta gatgtctaaa aatatacagt cactgccgag gacaagctag 3240 tgctccagacaccattctcc catccccctc tgatagagct gagtcacaaa acgacggtcg 3300 tctctcatgggcaggtttcc ttcctctctc ttcaatcccg tattgccatt tctcccacct 3360 gagaggaaggaacaaagttt agtgtggtat ccccttctag acacgcatag aacttacgcc 3420 ctttagtcctcactccgtgg gtaaattaga tgcatgttga ccattcccgt gatttgtata 3480 acgcacccttgtccatttca tgacctttca tgtccgtagc ttaaaaaata gaaagtgatt 3540 aatgataatggcagtgattg gttttaaggg ttttcctaaa ataatactaa ttataataat 3600 aatcataaaagggatctcta gactttaggc tttcagctta tccagatcca cttaattaga 3660 gaggaaaaaaattaccttaa atgatgtagc atttcacctt aaatttagag gcacggtgat 3720 agttgccctactctgcgctt aagctgacct tagacctcgt atcgtaagta gagtgagtga 3780 gtgagtgctctgaaagggaa agaacaaggt ctcctctgtg ttggtttcta tgtagtgcta 3840 tagaattagctttctcatac ccttgcccta ggatctacat aattcccttc ccattagggt 3900 agtatgctatgtataggaca ttttatcacc agacacaacg ctactgccca aaagtgaaaa 3960 acacacacttctggtcaatc aacaccacca gcaaatattt aaaaaaaata aaaaaggaag 4020 gaagaaagaaagaaagaaag aaagaaagaa agagagagag agagagaaag agagaaagaa 4080 agagaaagattgattctcag agtcttttcc taacagggtt acattgcttc agttggtgga 4140 tgtccctcccctgaaggaaa gacagaaaca ttattcaggg tccaggaggg gtatgcacac 4200 ttctcccccacctcaagaag gcaggctgga aagtcagctc ttcgtgcact caactgtggc 4260 atccacatgacctgtctgcc tcgcgtgcca aacttgatgc agggtttgcc ctgtctctgc 4320 tcactcccaccaagctgtag aactgtctgt cctgtccccg cccaccccat cgcaagctct 4380 gtgctgataaggacatgatg cttctactga attcacattc tagcacgtgc ttcaatatag 4440 ctcaaggaaccggaaagaca cttcgttttt cctgacccat cccctcttga cccccccccc 4500 acactgtaagacattagtaa tattaagaaa ttaaaagtca ttaatcctat gtggttcttg 4560 tctgtggtctctatagctcc tgaggtgtgc att 4593 82 320 PRT Mus musculus Beta-sarcoglycan82 Met Ala Ala Ala Ala Ala Ala Ala Ala Ala Thr Glu Gln Gln Gly Ser 1 510 15 Asn Gly Pro Val Lys Lys Ser Met Arg Glu Lys Ala Val Glu Arg Arg 2025 30 Asn Val Asn Lys Glu His Asn Ser Asn Phe Lys Ala Gly Tyr Ile Pro 3540 45 Ile Asp Glu Asp Arg Leu His Lys Thr Gly Leu Arg Gly Arg Lys Gly 5055 60 Asn Leu Ala Ile Cys Val Ile Val Leu Leu Phe Ile Leu Ala Val Ile 6570 75 80 Asn Leu Leu Ile Thr Leu Val Ile Trp Ala Val Ile Arg Ile Gly Pro85 90 95 Asn Gly Cys Asp Ser Met Glu Phe His Glu Ser Gly Leu Leu Arg Phe100 105 110 Lys Gln Val Ser Asp Met Gly Val Ile His Pro Leu Tyr Lys SerThr 115 120 125 Val Gly Gly Arg Arg Asn Glu Asn Leu Val Ile Thr Gly AsnAsn Gln 130 135 140 Pro Ile Val Phe Gln Gln Gly Thr Thr Lys Leu Ser ValGlu Lys Asn 145 150 155 160 Lys Thr Ser Ile Thr Ser Asp Ile Gly Met GlnPhe Phe Asp Pro Arg 165 170 175 Thr His Asn Ile Leu Phe Ser Thr Asp TyrGlu Thr His Glu Phe His 180 185 190 Leu Pro Ser Gly Val Lys Ser Leu AsnVal Gln Lys Ala Ser Thr Glu 195 200 205 Arg Ile Thr Ser Asn Ala Thr SerAsp Leu Asn Ile Lys Val Asp Gly 210 215 220 Arg Ala Ile Val Arg Gly AsnGlu Gly Val Phe Ile Met Gly Lys Thr 225 230 235 240 Ile Glu Phe His MetGly Gly Asp Val Glu Leu Lys Ala Glu Asn Ser 245 250 255 Ile Ile Leu AsnGly Thr Val Met Val Ser Pro Thr Arg Leu Pro Ser 260 265 270 Ser Ser SerGly Asp Gln Ser Gly Ser Gly Asp Trp Val Arg Tyr Lys 275 280 285 Leu CysMet Cys Ala Asp Gly Thr Leu Phe Lys Val Gln Val Thr Gly 290 295 300 HisAsn Met Gly Cys Gln Val Ser Asp Asn Pro Cys Gly Asn Thr His 305 310 315320 83 1237 DNA Mus musculus Beta-sarcoglycan 83 gagagcgcaa gcgcgggctgcggcgggcca agatggcggc agcggcggcg gcggcggcag 60 cgaccgagca gcaagggtccaacgggccag tgaagaaatc catgcgggag aaggctgtgg 120 agcggaggaa tgttaataaagagcacaaca gcaatttcaa agctggatat atccccatcg 180 atgaggaccg gctccataagactgggctga gggggcgaaa gggcaactta gccatctgcg 240 tgatcgtcct cctgtttatcctggccgtca tcaatctact cattacactt gtcatctggg 300 cggtgatccg cattgggccaaatgggtgtg atagcatgga gttccacgag agcggtctgc 360 tgaggttcaa gcaagtgtctgacatgggcg tcatccaccc gctttataag agcacggtgg 420 gaggacggcg gaatgaaaacctggtcatca ctggcaacaa ccagccaatt gtcttccagc 480 aagggacgac caagctgagtgttgaaaaga acaagacctc catcaccagc gacatcggga 540 tgcagttttt tgacccaaggacacacaata tcctgttcag cacggactat gagacgcacg 600 agtttcatct gccaagtggggtgaaaagtt tgaacgttca gaaagcatcg actgaaagga 660 ttaccagcaa tgctaccagtgacttaaaca tcaaagtcga tgggcgtgca attgtgcgag 720 gcaacgaagg cgtcttcatcatgggcaaaa ccattgaatt tcacatggga ggagatgtgg 780 agttaaaagc tgaaaacagcatcatcctca acggcacggt gatggtcagc cctacgcgcc 840 tgcccagttc ctccagcggggaccagtcag ggagtggtga ctgggtgcgc tacaagctct 900 gcatgtgtgc agatggcacactcttcaaag tacaagtaac aggtcacaac atgggctgcc 960 aggtctcaga caacccttgtgggaacactc attagacggt gcatccgcag gctcagcgcg 1020 ctcagatctg acttgaccttccccttttta agaatgcatg tgaaccatgt ttgtacagcg 1080 tttgccataa cttgtaattattttacctgg ggagcaccac tgtgtctatt atgtactctc 1140 catatttaag ggatcttgagtgcctacatt ctaatacatt ttagtaagtt gcactgatac 1200 tcaacagttc tctaaaacaatgatgagaat taataaa 1237 84 251 PRT Homo sapiens CGI-78 protein 84 MetGly Ala Ala Val Phe Phe Gly Cys Thr Phe Val Ala Phe Gly Pro 1 5 10 15Ala Phe Ala Leu Phe Leu Ile Thr Val Ala Gly Asp Pro Leu Arg Val 20 25 30Ile Ile Leu Val Ala Gly Ala Phe Phe Trp Leu Val Ser Leu Leu Leu 35 40 45Ala Ser Val Val Trp Phe Ile Leu Val His Val Thr Asp Arg Ser Asp 50 55 60Ala Arg Leu Gln Tyr Gly Leu Leu Ile Phe Gly Ala Ala Val Ser Val 65 70 7580 Leu Leu Gln Glu Val Phe Arg Phe Ala Tyr Tyr Lys Leu Leu Lys Lys 85 9095 Ala Asp Glu Gly Leu Ala Ser Leu Ser Glu Asp Gly Arg Ser Pro Ile 100105 110 Ser Ile Arg Gln Met Ala Tyr Val Ser Gly Leu Ser Phe Gly Ile Ile115 120 125 Ser Gly Val Phe Ser Val Ile Asn Ile Leu Ala Asp Ala Leu GlyPro 130 135 140 Gly Val Val Gly Ile His Gly Asp Ser Pro Tyr Tyr Phe LeuThr Ser 145 150 155 160 Ala Phe Leu Thr Ala Ala Ile Ile Leu Leu His ThrPhe Trp Gly Val 165 170 175 Val Phe Phe Asp Ala Cys Glu Arg Arg Arg TyrTrp Ala Leu Gly Leu 180 185 190 Val Val Gly Ser His Leu Leu Thr Ser GlyLeu Thr Phe Leu Asn Pro 195 200 205 Trp Tyr Glu Ala Ser Leu Leu Pro IleTyr Ala Val Thr Val Ser Met 210 215 220 Gly Leu Trp Ala Phe Ile Thr AlaGly Gly Ser Leu Arg Ser Ile Gln 225 230 235 240 Arg Ser Ser Cys Val ArgThr Asp Tyr Leu Asp 245 250 85 1967 DNA Homo sapiens CGI-78 protein 85gtggggtcgc gttgccaccc cacgcggact ccccagctgg cgcgcccctc ccatttgcct 60gtcctggtca ggcccccacc ccccttccca cctgaccagc catgggggct gcggtgtttt 120tcggctgcac tttcgtcgcg ttcggcccgg ccttcgcgct tttcttgatc actgtggctg 180gggacccgct tcgcgttatc atcctggtcg caggggcatt tttctggctg gtctccctgc 240tcctggcctc tgtggtctgg ttcatcttgg tccatgtgac cgaccggtca gatgcccggc 300tccagtacgg cctcctgatt tttggtgctg ctgtctctgt ccttctacag gaggtgttcc 360gctttgccta ctacaagctg cttaagaagg cagatgaggg gttagcatcg ctgagtgagg 420acggaagatc acccatctcc atccgccaga tggcctatgt ttctggtctc tccttcggta 480tcatcagtgg tgtcttctct gttatcaata ttttggctga tgcacttggg ccaggtgtgg 540ttgggatcca tggagactca ccctattact tcctgacttc agcctttctg acagcagcca 600ttatcctgct ccataccttt tggggagttg tgttctttga tgcctgtgag aggagacggt 660actgggcttt gggcctggtg gttgggagtc acctactgac atcgggactg acattcctga 720acccctggta tgaggccagc ctgctgccca tctatgcagt cactgtttcc atggggctct 780gggccttcat cacagctgga gggtccctcc gaagtattca gcgcagctct tgtgtaagga 840ctgactacct ggactgatcg cctgacagat cccacctgcc tgtccactgc ccatgactga 900gcccagcccc agcccggggt ccattgccca cattctctgt ctccttctcg tcggtctacc 960ccactacctc cagggttttg ctttgtcctt ttgtgaccgt tagtctctaa gctttaccag 1020gagcagcctg ggttcagcca gtcagtgact ggtgggtttg aatctgcact tatccccacc 1080acctggggac ccccttgttg tgtccaggac tccccctgtg tcagtgctct gctctcaccc 1140tgcccaagac tcacctccct tcccctctgc aggccgacgg caggaggaca gtcgggtgat 1200ggtgtattct gccctgcgca tcccacccga ggactgaggg aacctagggg ggacccctgg 1260gcctggggtg ccctcctgat gtcctcgccc tgtatttctc catctccagt tctggacagt 1320gcaggttgcc aagaaaaggg acctagttta gccattgccc tggagatgaa attaatggag 1380gctcaaggat agatgagctc tgagtttctc agtactccct caagactgga catcttggtc 1440tttttctcag gcctgagggg gaaccatttt tggtgtgata aataccctaa actgcctttt 1500tttctttttt gaggtggggg gagggaggag gtatattgga actcttctaa cctccttggg 1560ctatattttc tctcctcgag ttgctcctca tggctgggct catttcggtc cctttctcct 1620tggtcccaga ccttggggga aaggaaggaa gtgcatgttt gggaactggc attactggaa 1680ctaatggttt taacctcctt aaccaccagc atccctcctc tccccaaggt gaagtggagg 1740gtgctgtggt gagctggcca ctccagagct gcagtgccac tggaggagtc agactaccat 1800gacatcgtag ggaaggaggg gagatttttt tgtagttttt aattggggtg tgggaggggc 1860ggggaggttt tctataaact gtatcatttt ctgctgaggg tggagtgtcc catcctttta 1920atcaaggtga ttgtgatttt gactaataaa aaagaatttg taaaaaa 1967 86 885 PRT Homosapiens KIAA0143 protein 86 Ala Gly Gly Gly Ser Cys Arg Pro Leu Gly CysVal Thr Ala Gly Ser 1 5 10 15 Ala Ser Ala Pro Ser Thr Leu Arg Pro SerPro Phe Ala Ser Ser Arg 20 25 30 Pro Pro Arg Pro Ser Asn Gly Arg His GlyAla Val Gly Ala Pro Cys 35 40 45 Ala Ala Pro Leu Ser Leu Gly Ala Ala AlaSer Ala Val Glu Ile Ala 50 55 60 Met Pro Thr Arg Val Cys Cys Cys Cys SerAla Leu Arg Pro Arg Tyr 65 70 75 80 Lys Arg Leu Val Asp Asn Ile Phe ProGlu Asp Pro Lys Asp Gly Leu 85 90 95 Val Lys Thr Asp Met Glu Lys Leu ThrPhe Tyr Ala Val Ser Ala Pro 100 105 110 Glu Lys Leu Asp Arg Ile Gly SerTyr Leu Ala Glu Arg Leu Ser Arg 115 120 125 Asp Val Val Arg His Arg SerGly Tyr Val Leu Ile Ala Met Glu Ala 130 135 140 Leu Asp Gln Leu Leu MetAla Cys His Ser Gln Ser Ile Lys Pro Phe 145 150 155 160 Val Glu Ser PheLeu His Met Val Ala Lys Leu Leu Glu Ser Gly Glu 165 170 175 Pro Lys LeuGln Val Leu Gly Thr Asn Ser Phe Val Lys Phe Ala Asn 180 185 190 Ile GluGlu Asp Thr Pro Ser Tyr His Arg Arg Tyr Asp Phe Phe Val 195 200 205 SerArg Phe Ser Ala Met Cys His Ser Cys His Ser Asp Pro Glu Ile 210 215 220Arg Thr Glu Ile Arg Ile Ala Gly Ile Arg Gly Ile Gln Gly Val Val 225 230235 240 Arg Lys Thr Val Asn Asp Glu Leu Arg Ala Thr Ile Trp Glu Pro Gln245 250 255 His Met Asp Lys Ile Val Pro Ser Leu Leu Phe Asn Met Gln LysIle 260 265 270 Glu Glu Val Asp Ser Arg Ile Gly Pro Pro Ser Ser Pro SerAla Thr 275 280 285 Asp Lys Glu Glu Asn Pro Ala Val Leu Ala Glu Asn CysPhe Arg Glu 290 295 300 Leu Leu Gly Arg Ala Thr Phe Gly Asn Met Asn AsnAla Val Arg Pro 305 310 315 320 Val Phe Ala His Leu Asp His His Lys LeuTrp Asp Pro Asn Glu Phe 325 330 335 Ala Val His Cys Phe Lys Ile Ile MetTyr Ser Ile Gln Ala Gln Tyr 340 345 350 Ser His His Val Ile Gln Glu IleLeu Gly His Leu Asp Ala Arg Lys 355 360 365 Lys Asp Ala Pro Arg Val ArgAla Gly Ile Ile Gln Val Leu Leu Glu 370 375 380 Ala Val Ala Ile Ala AlaLys Gly Ser Ile Gly Pro Thr Val Leu Glu 385 390 395 400 Val Phe Asn ThrLeu Leu Lys His Leu Arg Leu Ser Val Glu Phe Glu 405 410 415 Ala Asn AspLeu Gln Gly Gly Ser Val Gly Ser Val Asn Leu Asn Thr 420 425 430 Ser SerLys Asp Asn Asp Glu Lys Ile Val Gln Asn Ala Ile Ile Gln 435 440 445 ThrIle Gly Phe Phe Gly Ser Asn Leu Pro Asp Tyr Gln Arg Ser Glu 450 455 460Ile Met Met Phe Ile Met Gly Lys Val Pro Val Phe Gly Thr Ser Thr 465 470475 480 His Thr Leu Asp Ile Ser Gln Leu Gly Asp Leu Gly Thr Arg Arg Ile485 490 495 Gln Ile Met Leu Leu Arg Ser Leu Leu Met Val Thr Ser Gly TyrLys 500 505 510 Ala Lys Thr Ile Val Thr Ala Leu Pro Gly Ser Phe Leu AspPro Leu 515 520 525 Leu Ser Pro Ser Leu Met Glu Asp Tyr Glu Leu Arg GlnLeu Val Leu 530 535 540 Glu Val Met His Asn Leu Met Asp Arg His Asp AsnArg Ala Lys Leu 545 550 555 560 Arg Gly Ile Arg Ile Ile Pro Asp Val AlaAsp Leu Lys Ile Lys Arg 565 570 575 Glu Lys Ile Cys Arg Gln Asp Thr SerPhe Met Lys Lys Asn Gly Gln 580 585 590 Gln Leu Tyr Arg His Ile Tyr LeuGly Cys Lys Glu Glu Asp Asn Val 595 600 605 Gln Lys Asn Tyr Glu Leu LeuTyr Thr Ser Leu Ala Leu Ile Thr Ile 610 615 620 Glu Leu Ala Asn Glu GluVal Val Ile Asp Leu Ile Arg Leu Ala Ile 625 630 635 640 Ala Leu Gln AspSer Ala Ile Ile Asn Glu Asp Asn Leu Pro Met Phe 645 650 655 His Arg CysGly Ile Met Ala Leu Val Ala Ala Tyr Leu Asn Phe Val 660 665 670 Ser GlnMet Ile Ala Val Pro Ala Phe Cys Gln His Val Ser Lys Val 675 680 685 IleGlu Ile Arg Thr Met Glu Ala Pro Tyr Phe Leu Pro Glu His Ile 690 695 700Phe Arg Asp Lys Cys Met Leu Pro Lys Ser Leu Glu Lys His Glu Lys 705 710715 720 Asp Leu Tyr Phe Leu Thr Asn Lys Ile Ala Glu Ser Leu Gly Gly Ser725 730 735 Gly Tyr Ser Val Glu Arg Leu Ser Val Pro Tyr Val Pro Gln ValThr 740 745 750 Asp Glu Asp Arg Leu Ser Arg Arg Lys Ser Ile Val Asp ThrVal Ser 755 760 765 Ile Gln Val Asp Ile Leu Ser Asn Asn Val Pro Ser AspAsp Val Val 770 775 780 Ser Asn Thr Glu Glu Ile Thr Phe Glu Ala Leu LysLys Ala Ile Asp 785 790 795 800 Thr Ser Gly Met Glu Glu Gln Glu Lys GluLys Arg Arg Leu Val Ile 805 810 815 Glu Lys Phe Gln Lys Ala Pro Phe GluGlu Ile Ala Ala Gln Cys Glu 820 825 830 Ser Lys Ala Asn Leu Leu His AspArg Leu Ala Gln Ile Leu Glu Leu 835 840 845 Thr Ile Arg Pro Pro Pro SerPro Ser Gly Thr Leu Thr Ile Thr Ser 850 855 860 Gly His Ala Gln Tyr GlnSer Val Pro Val Tyr Glu Met Lys Phe Pro 865 870 875 880 Asp Leu Cys ValTyr 885 87 5286 DNA Homo sapiens KIAA0143 protein 87 ggctggcggcggtagctgtc gcccgcttgg ttgcgtgacc gcggggtccg cgtccgctcc 60 ctccacccttcgcccttcgc ccttcgcctc gtcccggcct ccgcggccca gcaacggccg 120 tcatggtgccgtcggcgctc cctgcgcggc cccgctgagc ctcggtgcgg cggcgagcgc 180 ggtcgagatcgccatgccta cccgagtatg ctgctgctgt tccgctttgc gtcctcgcta 240 caaacgcctggtggacaaca tattccctga agatccaaaa gatggccttg tgaaaactga 300 tatggagaaattgacatttt atgcagtatc tgctccagag aaactggatc gaattggttc 360 ttacctggcagaaaggttga gcagggatgt tgtcagacat cgttctgggt atgttttgat 420 tgctatggaggcactggacc aacttctcat ggcttgccat tctcaaagca ttaagccatt 480 tgtagaaagctttcttcata tggtggcaaa gctgctggaa tcgggggaac caaagcttca 540 agttcttggaacaaattctt ttgtcaaatt tgcaaatatt gaagaagaca caccatccta 600 tcacagacgttatgactttt ttgtgtctcg attcagtgcc atgtgccatt cctgtcatag 660 tgatccagaaatacgaacag agatacgaat tgctggaatt agaggtattc aaggtgtggt 720 tcgcaaaacagtcaacgatg aacttcgggc caccatttgg gaacctcagc atatggataa 780 gattgttccatccctcctgt ttaacatgca aaagatagaa gaagttgaca gtcgcatagg 840 ccctccttcttctccttctg caactgacaa agaagagaat cctgctgtgc tggctgaaaa 900 ctgtttcagagaactgctgg gtcgagcaac ttttgggaat atgaataatg ctgttagacc 960 agtttttgcgcatttagatc atcacaaact gtgggatccc aatgaatttg cagttcactg 1020 ctttaaaattataatgtatt ccattcaggc tcagtattct caccatgtga tccaggagat 1080 tctaggacaccttgatgctc gtaaaaaaga tgctccccgg gttcgagcag gtattattca 1140 ggttctgttagaggctgttg ccattgctgc taaaggttcc ataggtccga cagtgctgga 1200 agtcttcaatacccttttga aacatctgcg tctcagcgtt gaattcgaag caaatgattt 1260 acaggggggatctgtaggca gtgtcaactt aaatacaagt tccaaagaca atgatgagaa 1320 gattgtgcagaatgctatca tccaaacaat aggatttttt ggaagtaacc taccagatta 1380 tcagaggtcagaaatcatga tgttcattat ggggaaagta cctgtctttg gaacatctac 1440 ccatactttggatatcagtc aactagggga tttgggaacc aggagaattc agataatgtt 1500 gctgagatctttgcttatgg tgacctctgg atataaagcg aagacgattg ttactgcact 1560 gccagggtctttcctggatc ctttgttatc accatctctc atggaggact acgaactgag 1620 acagttggtcttggaagtaa tgcataatct catggatcgt catgacaata gggcaaagct 1680 tcgagggatcagaataatac cggatgtagc tgacctaaag ataaaaagag aaaaaatttg 1740 cagacaagacacaagtttca tgaaaaagaa tgggcaacag ctgtatcggc acatatattt 1800 gggttgtaaagaggaagaca acgttcagaa aaactatgaa ctactttata cttctcttgc 1860 tcttataactattgaactgg ctaatgaaga agtagttatt gatctcattc gactggccat 1920 tgctttacaggacagtgcaa ttatcaatga ggataatttg ccaatgttcc atcgttgtgg 1980 aatcatggcactggttgcag catacctcaa ctttgtaagt cagatgatag ctgtccctgc 2040 attttgccagcatgttagca aggttattga aattcgaact atggaagccc cttattttct 2100 accagagcatatcttcagag ataagtgcat gcttccaaaa tctttagaga agcatgaaaa 2160 agatttgtactttctgacca acaagattgc agagtcgcta ggtggaagtg gatatagtgt 2220 tgagagattgtcagttccgt atgtaccaca agtaacagat gaagatcgac tttctagaag 2280 aaaaagcattgtggacaccg tatccattca ggtggatatt ttatccaaca atgttccttc 2340 tgatgatgtggttagtaaca ctgaagaaat cacttttgaa gcattgaaga aagcaattga 2400 taccagtggaatggaagaac aggaaaagga aaagaggcgt cttgtgatag agaaatttca 2460 gaaagcaccttttgaagaaa tagcagcaca gtgtgaatcc aaagcaaatt tgcttcatga 2520 tagacttgcccaaatattgg aactcaccat acgtcctcct cccagtccat caggaacact 2580 gaccattacttctgggcatg cccaatacca atctgtccca gtctatgaga tgaagtttcc 2640 agatctgtgtgtgtactgat cggcgcatga agacctcagc atatgatttg taaagcctaa 2700 aaattaaggccaagctgagc tttcagggtt tacttaatgt gtattaacat acttcttgaa 2760 aataatgatggaacatatct ttaaccaaat gtttggcata ccatattaga agttttggag 2820 ctatatataatttcgagtac tttcaaagat agatttatgc catgttaatt tgctttgagg 2880 ttcctgttgcctttttaagt tgaacatgtt ttggtttcac tttattccac tgttaagtag 2940 tatgttttaaacttttcaca aatgtaatgt tttttaaaaa gtaagccttc agaggattga 3000 aactgtataaattgtttatc tcttaaacat ctacacagcc gcttagatgt agaatttttg 3060 ttgttgttttctgcaaaggc agatacattt aaatatattc ctagtcctgg ggctgcaaaa 3120 ctgttcggtggctttttgtc cccatgcttt agataagctg ggataggcac cttgctattc 3180 agttactataataatatgtg ataggcattc ctcatcttct tcacaataat aggacatctg 3240 ttgaatagcattcctcgaat ataaccctaa aaacgccata ctttaaattg tctggttctt 3300 gtaatattgtgtttcctgcc aagagtttga ccattctgct tgagaagtgt agagcttact 3360 cttggagtaccaaactgtgc aatattttta catcataaga tgtattagtt tacaggctgt 3420 gctttgaaattatagtagta ttttgctgtg gctccattaa ttaaatgaga tatatattta 3480 gtgcagaaaaaagacattta aaacagctat tagttcacct gtgaagagtc tgtacatttt 3540 gatttctattaagagcacat ttatttacat ttgtatatta ttttaaatcc tcatagtcaa 3600 aaaattctccagatggactt ttaatttgta agatttgaac tgtgacttgt atacatctgt 3660 ttagaatcaattatatttga aaagctgcct gtgttttaac agtcaagtgt gctaaagttt 3720 gtcaatttaagctgcttttg atttcagcta ccaagattac aggtgcactc tacacataac 3780 actgacagacccataacatt acatacatct tagtgaattc tatcacatgg taaaatgaac 3840 agctttctttgtaactcata aaattctctt aggacatttt tataaagtca cctgtttata 3900 gttctatcttttcagattcc atttcttttt acataaaaca gcatacatat caaaaactgt 3960 agcctagaatacagtttaat ttttggcttt tgtttttgtt taaaaaattg cagtgaagaa 4020 tgggatgtttgtgtttatgg ctatttgggc acctttagta gaaacagaca aaagtaagga 4080 aacgataataggacaagcat acttgaaaat ttctgaatac ttaaacaaaa gcgcaacatc 4140 ttgaaaaccagtctagtcat tgaaacctat gaaatgacac tgaaagatcc tgggctgctt 4200 cttaaataagtagatcagca agacttgttt cagagtgaca gtggagtcgt taccgctgga 4260 ggactaaagggccctgtggc agctgtcact ggaactttgc ctcttgatca ggaaaaatgc 4320 ttcaccagtccgtaaagcca agttgtattt tttttattgc ccttttttcc ttctgtattt 4380 ttaaagaaggatgttaattt ttgactatat attttaaaaa aatctaagca gggggacatg 4440 caaaaacaatcatcatccac ttggatgtca ttttatagat taacactgtg tgcttttgta 4500 tggaaaaaatatatataatt taatagtata aaaaataaaa tatatattca tttgcactta 4560 cgtgaaacacaaactttgct ctacaaaatt tcgtgtttct tagtgatttt aaaatgcatg 4620 tattgcatgtaaaggaaaac cattacaatt aatgtttatc acacctttat cttggtcttt 4680 gttgatttgggttttgttgg ggtttttgtt actgttttta aattacagta ggcttctata 4740 tatcctggatttctgaactg gtcttgttga caaggattcc caagaaatgg atcttttcac 4800 tggctgactctcccatatct gcaagagatt ctgcaggaac tgggtgtgca cacggtgttg 4860 tagccagttcaggtactgaa tatttaggat ttggtgaagt tcactgtatt gctatatttt 4920 tgtagataaataaaactcaa taaattgtta atcattctct ttttgctgta tagaattgct 4980 tatatcactctttctttcat gacattggtt aacatttaaa tgttcctcct gtacttgtgt 5040 tgtctgtgaccgcttataga gttttattgt tattggtgtt tacctgaata cctatgcgta 5100 cacacacacatatttcctta atacttctga actcattatc ttttagaata ataatactac 5160 tacactttaccagcaattaa cttctcccta cccaaaatgt ttttcttcct gtctgaaaat 5220 ggaactaatttgtcttattc gtgcttatat ctgtattaaa tgcaataaag ttagtttttg 5280 aaatgt 528688 424 PRT Homo sapiens Septin 2 88 Thr Asp Ile Ala Arg Gln Val Gly GluGly Cys Arg Thr Val Pro Leu 1 5 10 15 Ala Gly His Val Gly Phe Asp SerLeu Pro Asp Gln Leu Val Asn Lys 20 25 30 Ser Val Ser Gln Gly Phe Cys PheAsn Ile Leu Cys Val Gly Glu Thr 35 40 45 Gly Leu Gly Lys Ser Thr Leu MetAsp Thr Leu Phe Asn Thr Lys Phe 50 55 60 Glu Gly Glu Pro Ala Thr His ThrGln Pro Gly Val Gln Leu Gln Ser 65 70 75 80 Asn Thr Tyr Asp Leu Gln GluSer Asn Val Arg Leu Lys Leu Thr Ile 85 90 95 Val Ser Thr Val Gly Phe GlyAsp Gln Ile Asn Lys Glu Asp Ser Tyr 100 105 110 Lys Pro Ile Val Glu PheIle Asp Ala Gln Phe Glu Ala Tyr Leu Gln 115 120 125 Glu Glu Leu Lys IleArg Arg Val Leu His Thr Tyr His Asp Ser Arg 130 135 140 Ile His Val CysLeu Tyr Phe Ile Ala Pro Thr Gly His Ser Leu Lys 145 150 155 160 Ser LeuAsp Leu Val Thr Met Lys Lys Leu Asp Ser Lys Val Asn Ile 165 170 175 IlePro Ile Ile Ala Lys Ala Asp Ala Ile Ser Lys Ser Glu Leu Thr 180 185 190Lys Phe Lys Ile Lys Ile Thr Ser Glu Leu Val Ser Asn Gly Val Gln 195 200205 Ile Tyr Gln Phe Pro Thr Asp Asp Glu Ser Val Ala Glu Ile Asn Gly 210215 220 Thr Met Asn Ala His Leu Pro Phe Ala Val Ile Gly Ser Thr Glu Glu225 230 235 240 Leu Lys Ile Gly Asn Lys Met Met Arg Ala Arg Gln Tyr ProTrp Gly 245 250 255 Thr Val Gln Val Glu Asn Glu Ala His Cys Asp Phe ValLys Leu Arg 260 265 270 Glu Met Leu Ile Arg Val Asn Met Glu Asp Leu ArgGlu Gln Thr His 275 280 285 Thr Arg His Tyr Glu Leu Tyr Arg Arg Cys LysLeu Glu Glu Met Gly 290 295 300 Phe Lys Asp Thr Asp Pro Asp Ser Lys ProPhe Ser Leu Gln Glu Thr 305 310 315 320 Tyr Glu Ala Lys Arg Asn Glu PheLeu Gly Glu Leu Gln Lys Lys Glu 325 330 335 Glu Glu Met Arg Gln Met PheVal Gln Arg Val Lys Glu Lys Glu Ala 340 345 350 Glu Leu Lys Glu Ala GluLys Glu Leu His Glu Lys Phe Asp Arg Leu 355 360 365 Lys Lys Leu His GlnAsp Glu Lys Lys Lys Leu Glu Asp Lys Lys Lys 370 375 380 Ser Leu Asp AspGlu Val Asn Ala Phe Lys Gln Arg Lys Thr Ala Ala 385 390 395 400 Glu LeuLeu Gln Ser Gln Gly Ser Gln Ala Gly Gly Ser Gln Thr Leu 405 410 415 LysArg Asp Lys Glu Lys Lys Asn 420 89 4612 DNA Homo sapiens Septin 2 89cgaccgatat agctcgccag gtgggtgaag gttgccgaac tgtccccctg gctggacatg 60tggggtttga cagcttgcct gaccagctgg tgaataagtc cgtcagccag ggcttctgct 120tcaacatcct gtgcgtggga gagacaggtt tgggcaagtc caccctcatg gacaccctgt 180tcaacaccaa attcgaaggg gagccagcca cccacacaca gccgggtgtc cagctccagt 240ctaataccta tgacctccaa gagagcaacg tgaggctaaa gctcacgatc gttagcacag 300ttggctttgg ggaccagatc aacaaagagg acagctacaa gcctatcgtg gaattcatcg 360atgcacaatt cgaggcctac ctgcaggaag agctaaagat ccgaagagtg ctacacacct 420accatgactc ccgaatccat gtctgcttgt atttcattgc ccccacgggt cattccctga 480agtctctgga cctagtgact atgaagaagc tggacagtaa ggtgaacatc atccccatca 540ttgccaaagc agatgccatt tcgaagagtg agctaacaaa gttcaaaatc aaaatcacca 600gcgagcttgt cagcaacgga gtccagatct atcagtttcc tacagatgat gagtcggtgg 660cagagatcaa tggaaccatg aacgcccacc tgccgtttgc tgtcattggc agcacagaag 720aactgaagat aggcaacaag atgatgaggg cgcggcagta tccttggggc actgtgcagg 780ttgaaaacga ggcccactgc gactttgtga agctgcggga gatgctgatt cgggtcaaca 840tggaggatct gcgggagcag acccacaccc ggcactatga gctgtatcgc cgctgtaagc 900tggaggagat gggcttcaag gacaccgacc ctgacagcaa acccttcagt ttacaggaga 960catatgaggc caaaaggaac gagttcctag gggaactcca gaaaaaagaa gaggagatga 1020gacagatgtt cgtccagcga gtcaaagaga aagaagcgga gctcaaagag gcagagaaag 1080agctgcacga gaagtttgac cgtctgaaga aactgcacca ggacgagaag aagaaactgg 1140aggataagaa gaaatccctg gatgatgaag tgaatgcttt caagcaaaga aagacggcgg 1200ctgagctgct ccagtcccag ggctcccagg ctggaggctc acagactctg aagagagaca 1260aagagaagaa aaattaactc tgctgtttgc tgcatgctgc atgagaccca gggtcctgtt 1320tgggcttcct gtagacaccc ttttcctgcg caacagagct gggcctccct ttctctaatt 1380tcccccttaa catgcctggg gggcatacaa tccaacccgc gccctctcct ctcttcctgc 1440caaggtttat agaaacctga gaatctgagg gtgatgtctg gccgctggtc aagaagccaa 1500cagtcatgtg gctcgcagat gcatcctgca tcccagtccc cctcccagca cccccagcca 1560tcccccctgt cttcccccac atctttgcca gaggtgtgac atggtcaggg ggcccatctg 1620ctactctttc ccaccagctc ccctgttcca gttctggttg ctgttagttt ccctgaggta 1680tttgcaacca ccatggctgg gtaaccaccg atcagcacag ctgtcccctt ggtctcctgt 1740atcccagtca ctagtcctcc ctggtccacc ccaccctcat cctcaggagc cacagccatt 1800tcttagaggg tttcaaaagg acagcctttg gcgccttttc cttctaacct ttgagtccag 1860ccctttccag ttttcattca ctcgaagtaa ctgcactcaa gctgtgctca aaatcggcaa 1920cgcatttatt tacaccaagc ccttcccata aaacacaact gctgaagaaa atagcagacg 1980tttcccctct ctctaactct gggtatccca cagatgcaaa agggagaata aacctgaata 2040ttattaccag cctagagtct tgaatgatag ccttaccgaa ttcttcttgt gaggtatttc 2100agcatctcgg ggggtaattt ccggaagggc tccatactgt cccaataagg tgaggccagt 2160agcaggaata ataaatccca ctttgtaggc tggaaaactg agctgtcaaa agaatcaagt 2220gtttgggggt ttgctctgat gagtcttcta gttcatttgg tgaatgtcat gatgattttt 2280aacatgcatt ttgcatgcat cccccaataa gaagagatga gactcggctg gagagaagaa 2340aaggccctta actttctttc caatttaagg agttgagagt ttaaaaatat tccagcccta 2400agtttttatc atgggtccca tctgatagtg gctttgggaa cctctgtgaa gtagagagcc 2460ctcccttgtc agggttatga ggcacagtgg cctttggtgt ttggccagtg acagtgtgag 2520agatggagtt gacctggcaa tgatctgtgg ctaacatgcc gtctctctgc ccttcctttg 2580cagtaatcca tggctgtgta ctgaatagta ttccccgcta cagctggact ggactccatt 2640tagcctttta agccgaggtt cctattttaa ctgacagctt tcctttgggg tgccaggcag 2700cgaggccccc cacccctatc ctgccatgta cttcaagctc acttcttctt tttgagttcc 2760gcaacttgct cctgcctccc agccccactg gcactgacca tgaccaccta cttctatttt 2820ttttttagag tttctttttt tgatcactta ctttcaaagc acacagtcaa acaaggttat 2880gccaaatttc caggcctttt tgaagtattg agaaggggaa ggggatttct cacttcaatt 2940atagatcata ataggaagca aaaagaaaaa aatgaaaagc aaacatatgc acgcactttt 3000cttgttgaca aagcaagaat ataggtttgc tgtgtaggtt tggtgctcta ttgattggtg 3060agtgaccaga gcaagtatga aggtgatgct gccaaagcac aagccttttt gaagtattga 3120gaaggggaag gggatttctc acttcaatta tagatcataa taggaagcaa aaagaaaaaa 3180atgaaaagca aacatatgca cgcacttttc ttgttgacaa agcaagaata taggtttgct 3240gtgtaggttt ggtgctctat tgattggtga gtgaccagag caagtatgaa ggtgatgctg 3300ccaaagcaca agccagtttc ttgggaaaat tcaagttaca gtggagtatt tttttgaaga 3360ccatatgctt ggaggtagaa acaaaccaac gaccaaaaaa aaaaaaaaaa aatctgctca 3420gatactcagc cagtagctca gagagatgct gagttaggcc tgtcaggtct ccttgggaaa 3480ggcttcatat ttgcaacttt gatgattcta tgtccagctt cagagctgct ttcccagaaa 3540ttcacgctta aacaaccaac cggtaaccac cacttcccca caccgccgcc cggtaattat 3600ttgcattaca aaccggaggc gccctcattt gcatttgtgt acagattaac tagttaaggc 3660ttgagaagct ctgaataatt caaaagtatt agacccacac agccttggag agaccttcag 3720aaactaagga ggagttttat attaagggag acattttagt cagtaagacg atataaccta 3780cttactccgt aaggggaaat gaaggcccag agaagggaag ggacttgacc gaggtcccac 3840ttctgtttcg aggcagaagc cagactaatt ttcatgcctc ctgactccca atcagtttca 3900caaagggatt caatctgttt atatacgtta cattcctgga tacgaggtct tttgatgttc 3960agagtaactg actagttagt attagaagac cctcgaggtt tttttccaca gaaaaacatc 4020tgaagatgga ttgggtgagg gctggcaaaa cgaaggcatg ccgggccagc tccttaaccc 4080aatgacccag tgatgctgca aggctggaac ggggtccagg agactgtgtg taacaggtgc 4140cctaggtgac ccttataatc agggaagttt ggtgaacaaa aatcgaaccc atgagtgaac 4200ataaattaaa aagttgatca acctattaaa atgtgtattt cattgggtag cttttctcac 4260tgtagacaga ttttttcctt cttcaatgaa aaggctttta aattagtaca actgttacta 4320tttaaaaaaa aaatacccta agtactctgt ttacttctgg tgaaacaaaa ccagtcatta 4380gaaatggtct gtgcttttat tttcccagac tggagtggct tttctgaaac acacacacac 4440acacacacac acacacacac gtacacacat ccctcacttc tcttaagcca agaagtttgc 4500tttccctagc tgcagtgtag atggctcttg tttttgtttt tttgttttaa tcatttggca 4560ttcacatgtg gctgttaata tgtgcttgtt tttaattaaa acaagaagct tt 4612 90 459PRT Rattus norvegicus Nucleobindin 90 Met Pro Thr Ser Val Pro Arg GlyAla Pro Phe Leu Leu Leu Pro Pro 1 5 10 15 Leu Leu Met Leu Ser Ala ValLeu Ala Val Pro Val Asp Arg Ala Ala 20 25 30 Pro His Gln Glu Asp Asn GlnAla Thr Glu Thr Pro Asp Thr Gly Leu 35 40 45 Tyr Tyr His Arg Tyr Leu GlnGlu Val Ile Asn Val Leu Glu Thr Asp 50 55 60 Gly His Phe Arg Glu Lys LeuGln Ala Ala Asn Ala Glu Asp Ile Lys 65 70 75 80 Ser Gly Lys Leu Ser GlnGlu Leu Asp Phe Val Ser His Asn Val Arg 85 90 95 Thr Lys Leu Asp Glu LeuLys Arg Gln Glu Val Ser Arg Leu Arg Met 100 105 110 Leu Leu Lys Ala LysMet Asp Ala Lys Gln Glu Pro Asn Leu Gln Val 115 120 125 Asp His Met AsnLeu Leu Lys Gln Phe Glu His Leu Asp Pro Gln Asn 130 135 140 Gln His ThrPhe Glu Ala Arg Asp Leu Glu Leu Leu Ile Gln Thr Ala 145 150 155 160 ThrArg Asp Leu Ala Gln Tyr Asp Ala Ala His His Glu Glu Phe Lys 165 170 175Arg Tyr Glu Met Leu Lys Glu His Glu Arg Arg Arg Tyr Leu Glu Ser 180 185190 Leu Gly Glu Glu Gln Arg Lys Glu Ala Glu Arg Lys Leu Gln Glu Gln 195200 205 Gln Arg Arg His Arg Glu His Pro Lys Val Asn Val Pro Gly Ser Gln210 215 220 Ala Gln Leu Lys Glu Val Trp Glu Glu Leu Asp Gly Leu Asp ProAsn 225 230 235 240 Arg Phe Asn Pro Lys Thr Phe Phe Ile Leu His Asp IleAsn Ser Asp 245 250 255 Gly Val Leu Asp Glu Gln Glu Leu Glu Ala Leu PheThr Lys Glu Leu 260 265 270 Glu Lys Val Tyr Asp Pro Lys Asn Glu Glu AspAsp Met Arg Glu Met 275 280 285 Glu Glu Glu Arg Leu Arg Met Arg Glu HisVal Met Lys Asn Val Asp 290 295 300 Thr Asn Gln Asp Arg Leu Val Thr LeuGlu Glu Phe Leu Ala Ser Thr 305 310 315 320 Gln Arg Lys Glu Phe Gly GluThr Ala Glu Gly Trp Lys Thr Val Glu 325 330 335 Met Tyr Pro Ala Tyr ThrGlu Glu Glu Leu Lys Arg Phe Glu Glu Glu 340 345 350 Leu Ala Ala Arg GluAla Glu Leu Asn Ala Arg Ala Gln Arg Leu Ser 355 360 365 Gln Glu Thr GluAla Leu Gly Arg Ser Gln Asp Arg Leu Glu Ala Gln 370 375 380 Lys Arg GluLeu Gln Gln Ala Val Leu Gln Met Glu Gln Arg Lys Gln 385 390 395 400 GlnGln Gln Glu Gln Ser Ala Pro Pro Ser Gln Pro Asp Gly Gln Leu 405 410 415Gln Phe Arg Ala Asp Thr Gly Asp Ala Pro Val Pro Ala Pro Ala Gly 420 425430 Asp Gln Lys Asp Val Pro Ala Ser Glu Lys Lys Val Pro Glu Gln Pro 435440 445 Pro Val Leu Pro Gln Leu Asp Ser Gln His Leu 450 455 91 2152 DNARattus norvegicus Nucleobindin 91 ccagaaacct ggcggaggtc gcgggctgggaaaacgccct ctgcgctagg agagaccagc 60 tacgatgcct acctctgtgc cccgcggggcgcctttcctt ctcctaccac ctttactgat 120 gctgtctgct gtgctggcag tgcctgtggaccgcgcagca cctcatcagg aggacaacca 180 ggccactgag accccggaca caggcctgtactaccatcgg tacctccagg aggtcatcaa 240 cgtgctagag acagatgggc acttccgggagaagctgcaa gctgccaacg ctgaggacat 300 taagagtgga aagctgagtc aagagctggacttcgtcagc cacaacgtcc gcaccaagct 360 ggatgagctc aagcgacagg aggtatcaaggcttcggatg ctgctcaagg ccaagatgga 420 tgcaaagcag gagcctaact tgcaggtggaccacatgaac cttctgaagc agtttgaaca 480 cctggaccct cagaaccagc acacgtttgaggctcgggac ctagagctgc tgatccagac 540 ggccacccga gacctcgccc agtatgacgctgcacaccat gaagagttca aacgctacga 600 gatgctcaag gaacatgagc gaagacgttacctggagtct ctgggagagg agcagcggaa 660 ggaggctgag aggaagctac aagagcagcagcgcagacac cgggaacacc ccaaagtcaa 720 tgttcctggc agccaagccc agttgaaggaggtgtgggag gagctagatg gattggaccc 780 caacaggttc aaccccaaga ccttcttcatactgcatgac atcaacagcg atggtgtcct 840 agatgagcaa gaactggaag ctctgtttaccaaggagctg gagaaggtgt acgacccgaa 900 gaacgaggag gacgacatga gggagatggaggaggagcgg ctgcgcatgc gggagcatgt 960 gatgaagaac gtggacacca accaggaccgacttgtgacc ctggaggaat tcctggcatc 1020 cacacagagg aaggagtttg gggaaactgcggagggatgg aagacagtgg aaatgtatcc 1080 agcctacaca gaggaagagc tgaagcgttttgaagaggag cttgctgccc gggaggctga 1140 gctaaatgcc agagcccagc gcctcagccaggagacagag gccctggggc gctcccagga 1200 ccgcctagag gctcagaaga gagagctgcagcaggccgtt ctgcagatgg agcaaaggaa 1260 gcagcaacag caagagcaga gcgctccgccttcccaacct gatgggcagc tgcagttccg 1320 tgcagacaca ggggatgctc ctgtcccagctccagcaggt gaccagaaag acgtgcctgc 1380 ttctgaaaag aaggttccag agcagccccctgtgctgcca cagctggatt ctcagcattt 1440 ataagccctc agagatcccg gccctcaagttcccacagac agtgatggaa gctggatgaa 1500 gtgtccttgc agcctttctg gagaccaaagccttcatggc ctccagggga gaacagggcc 1560 accctgttcc tcagatgcgc ttctgcccatgtctaccagt ctgttctcac agcctctggg 1620 tcccagtcct gccttcctcc ctcccttccttggcagggac tcaactgcag gagcccctca 1680 ccccacctga gtgcacaccc caggcctgcctgccttccat gctttgctct tacctgtccc 1740 aggctcaacc tttctgcttc ctgtacctgtcagtgggtca caggagcttg acttggtctc 1800 tccaactctt tgccttgtga gaggccaatgatctcaattc cttttggtgg ctagaggccg 1860 ccacaatgtg gggtgtccta tcagctctgcctgggactcc tgaactccac caccacccca 1920 ccagccttac cctaaccacc tgggcccaatacctacctga gggtcccaga aggggcccag 1980 atatggctac accccttact gaggtccagcctcactccca cgtatggggg ctcctgaaaa 2040 ttggttcacc cacttccctg gcttcaggccacactgctgt gtcctgctct gtgtcccagt 2100 ctcatccaac tatagcctgg cacaaataaacattagatat ctgtgtgaat gt 2152 92 160 PRT Rattus norvegicus Myelinstructural protein SR 13 92 Met Leu Leu Leu Leu Leu Gly Ile Leu Phe LeuHis Ile Ala Val Leu 1 5 10 15 Val Leu Leu Phe Val Ser Thr Ile Val SerGln Trp Leu Val Gly Asn 20 25 30 Gly His Arg Thr Asp Leu Trp Gln Asn CysThr Thr Ser Ala Leu Gly 35 40 45 Ala Val Gln His Cys Tyr Ser Ser Ser ValSer Glu Trp Leu Gln Ser 50 55 60 Val Gln Ala Thr Met Ile Leu Ser Val IlePhe Ser Val Leu Ser Leu 65 70 75 80 Phe Leu Phe Phe Cys Gln Leu Phe ThrLeu Thr Lys Gly Gly Arg Phe 85 90 95 Tyr Ile Thr Gly Val Phe Gln Ile LeuAla Gly Leu Cys Val Met Ser 100 105 110 Ala Ala Ala Ile Tyr Thr Val ArgHis Ser Glu Trp His Val Asn Asn 115 120 125 Asp Tyr Ser Tyr Gly Phe AlaTyr Ile Leu Ala Trp Val Ala Phe Pro 130 135 140 Leu Ala Leu Leu Ser GlyIle Ile Tyr Val Ile Leu Arg Lys Arg Glu 145 150 155 160 93 1736 DNARattus norvegicus Myelin structural protein SR 13 93 gggggaagccagcaacctag aggacgcccc cgagtttgtg cctgaggcta ctccgctctg 60 agccggctgtccctttgaac tgaaagcacc gctccaccga gcccgagccc aactccagcc 120 accatgcttctactcttgtt ggggatcctg ttccttcaca tcgcggtgct agtgttgctc 180 ttcgtctccaccatcgtcag ccaatggctc gtgggcaatg gacacaggac tgatctctgg 240 cagaactgtaccacatccgc cttgggagcc gtccagcact gctactcctc atctgtgagc 300 gaatggcttcagtctgtcca ggccaccatg atcctgtctg tcatcttcag cgtcctgtcc 360 ctgttcctgttcttctgcca gctcttcact ctcaccaaag gcggccgctt ttacatcact 420 ggagtcttccaaatccttgc tggtctgtgt gtgatgagtg cagcggccat ctacacagtg 480 agacacagtgagtggcatgt caacaacgac tactcctatg gctttgctta catcctggcc 540 tgggtggctttcccgctggc cctccttagt ggcatcatct acgtgatcct gcggaaacgc 600 gaatgaggcgcccgacgcac catccgtcta ggctctgagc gtgcataggg tacacaggga 660 gggaggaaggaaaccagaaa accaaaccaa ccaacccaaa agagctagcc cccaaaccca 720 aacgcaagccaaaccaaaca gaacacagtt gagtggggat tgctgtcgat tgaagatgta 780 tataatatctatggtttata aaacctattt ataacacttt ttacatacat gtacatagga 840 ttgtttgctttttatgttga ccgtcagcct cgtgttgaat cttaaacgac tctacatcct 900 aacactataaccaagctcag tattttcgtt ttgtttcgtt tttttcatct ttttgttttg 960 ctcagacataaaaaaaaaaa aaaaatccac gtggccccct ttcatctgaa agcagatccc 1020 tccctcccattcaacctcat aggataacca aagtgcgggg acaaacccca gatggccaga 1080 ggccttcacactatgggtga cccagtgaat ttagcaggaa taatccgctg cccgaatcaa 1140 tgtgtgaagccctaagcact cacagacgaa acgccctgac cagagccctc tgcgaaacca 1200 atagctggtggctgcggaac acttgaccct gaaggcggag tactggggca catgtttaaa 1260 tgagacgtcagagacaagca atctgtgaaa tggtgctata gatttaccat tccttgttat 1320 tactaatcatttaaaccact cactggaaac tcaattaaca gttttatgac ctacagcaga 1380 acagagacccgatacaaacg gttcgtaact gctttcgtac atagctaggc tgttgttatt 1440 actacaataaataaatctca aagccttcgt cactcccaca gttttctcac ggtcggagca 1500 tcaggacgagggtctagacc cttgggacta gcaaattccc tggctttctg ggtctagagt 1560 gttctgtgcctccaaggact gtctagcgat gacttgtatt ggccaccaac tgtagatgta 1620 tatacggtgtccttctgatg ctaagactcc agacctttct tggttttgct ggctttttct 1680 gattttataccaactgtgtg gactaagatg cattaaaata aacatcagag taactc 1736 94 375 PRTRattus norvegicus Cytoplasmic beta-actin 94 Met Asp Asp Asp Ile Ala AlaLeu Val Val Asp Asn Gly Ser Gly Met 1 5 10 15 Cys Lys Ala Gly Phe AlaGly Asp Asp Ala Pro Arg Ala Val Phe Pro 20 25 30 Ser Ile Val Gly Arg ProArg His Gln Gly Val Met Val Gly Met Gly 35 40 45 Gln Lys Asp Ser Tyr ValGly Asp Glu Ala Gln Ser Lys Arg Gly Ile 50 55 60 Leu Thr Leu Lys Tyr ProIle Glu His Gly Ile Val Thr Asn Trp Asp 65 70 75 80 Asp Met Glu Lys IleTrp His His Thr Phe Tyr Asn Glu Leu Arg Val 85 90 95 Ala Pro Glu Glu HisPro Val Leu Leu Thr Glu Ala Pro Leu Asn Pro 100 105 110 Lys Ala Asn ArgGlu Lys Met Thr Gln Ile Met Phe Glu Thr Phe Asn 115 120 125 Thr Pro AlaMet Tyr Val Ala Ile Gln Ala Val Leu Ser Leu Tyr Ala 130 135 140 Ser GlyArg Thr Thr Gly Ile Val Met Asp Ser Gly Asp Gly Val Thr 145 150 155 160His Thr Val Pro Ile Tyr Glu Gly Tyr Ala Leu Pro His Ala Ile Leu 165 170175 Arg Leu Asp Leu Ala Gly Arg Asp Leu Thr Asp Tyr Leu Met Lys Ile 180185 190 Leu Thr Glu Arg Gly Tyr Ser Phe Thr Thr Thr Ala Glu Arg Glu Ile195 200 205 Val Arg Asp Ile Lys Glu Lys Leu Cys Tyr Val Ala Leu Asp PheGlu 210 215 220 Gln Glu Met Ala Thr Ala Ala Ser Ser Ser Ser Leu Glu LysSer Tyr 225 230 235 240 Glu Leu Pro Asp Gly Gln Val Ile Thr Ile Gly AsnGlu Arg Phe Arg 245 250 255 Cys Pro Glu Ala Leu Phe Gln Pro Ser Phe LeuGly Met Glu Ser Cys 260 265 270 Gly Ile His Glu Thr Thr Phe Asn Ser IleMet Lys Cys Asp Val Asp 275 280 285 Ile Arg Lys Asp Leu Tyr Ala Asn ThrVal Leu Ser Gly Gly Thr Thr 290 295 300 Met Tyr Pro Gly Ile Ala Asp ArgMet Gln Lys Glu Ile Thr Ala Leu 305 310 315 320 Ala Pro Ser Thr Met LysIle Lys Ile Ile Ala Pro Pro Glu Arg Lys 325 330 335 Tyr Ser Val Trp IleGly Gly Ser Ile Leu Ala Ser Leu Ser Thr Phe 340 345 350 Gln Gln Met TrpIle Ser Lys Gln Glu Tyr Asp Glu Ser Gly Pro Ser 355 360 365 Ile Val HisArg Lys Cys Phe 370 375 95 4100 DNA Rattus norvegicus misc_feature 3859n is a or g or c or t 95 acctcttcct caactcactt ctctctactc tcacttttttttttcttctt cttttttttt 60 tttttttttt tttttttttt tgcaaaagaa gggggtaaaaaaatgctgca ctgtcgggcg 120 aggccggtga gtgagcgagc cggagccaat cagcgcccgccgttccgaaa ttgcctttta 180 tggctcgagt ggccgctgtg gcgtcctata aaacccggcggcgcaacgcg cgccactgtc 240 gagtccgcgt ccacccgcga gtacaacctc cttgcagctcctccgtcccg gtccacaccc 300 gccaccaggt aagcagggac gtcgggccca gcgggccccaactttacctt ggccactacc 360 tcgctgcagg atcgtgagga acactcagaa gggacaccgtagaggggtgg agcgtggtac 420 cgggccgcgg agcagctggc aaagcttaac tttcccggcccctagggtgt agagtgtttg 480 cagtcgtatt cccgcggtgt ggacactcgt gggtacgctcctgcttggtg cgcggggctt 540 ggggacacac tagagtcgcg gtgtgggcat ttggagagccggtgcggctg cgggtgttaa 600 gccgcatctg tccaccttga ggggcaacag tattggggtcaggcgttaca atcacgcttt 660 gatggcctat gggtctttgt ccaaaccggt tttgccattcggcttggcgg gcgcggcggg 720 gccgctcggc cgggtggggg ctgggatgcc attgcgcgtgcgcgctctat cactgggcat 780 tggggccgtg cgcgctgggg agggaactct tcctctccccctcttccgag ttaagagttg 840 cgcgtgcgta ttgagactag gagcgcggcc gccccgggttgggcgagggc gggccgtcca 900 ccggaagggg cggggtcgta gcggctaggc gcctgctcgcgcttcctgct gggtgtggtc 960 gcctcccgcg cgcgcactag ccgcccgtcg cctcagtgtaggcgggcctg tgcccgtttg 1020 gggaggggcg gaggcctggc ttcctgccgt gggtccgcctccgggccagc gtttgccttt 1080 tatggtaata atgcggctgt cctgcgcttc ctttgtcccctgagcttggg cgcgcccctg 1140 gcggctcgag gccgcggctc gccggaagtg ggcagggcggcagcggctgc tcttggcggc 1200 tccgcggtga ccatagccct cttttgtgcc ttgatagttcgccatggatg acgatatcgc 1260 tgcgctcgtc gtcgacaacg gctccggcat gtgcaaggccggcttcgcgg gcgacgatgc 1320 tccccgggcc gtcttcccct ccatcgtggg ccgccctaggcaccaggtaa gtgacccttt 1380 actttgggag tggcagccct agggttttct tgggggtcgatgccagtgct gagaacgttg 1440 ttctcctccg cagggtgtga tggtgggtat gggtcagaaggactcctacg tgggcgacga 1500 ggcccagagc aagagaggca tcctgaccct gaagtaccccattgaacacg gcattgtaac 1560 caactgggac gatatggaga agatttggca ccacactttctacaatgagc tgcgtgtggc 1620 ccctgaggag caccctgtgc tgctcaccga ggcccctctgaaccctaagg ccaaccgtga 1680 aaagatgacc caggtcagta tcctgggtga ccctccccttcttattgggt caacttctca 1740 gcacgccctt ctctaattgt ctttcttctg ccatgtcccataggactctc ttctatgagc 1800 tgagtctccc ttggaacttt gcagtttctg ctctttcccagatgaggtct tttttttctc 1860 tcgatcgcct ttctgactag gtgttttaaa cccctacagtgctgtgggtg taggtactaa 1920 caatggctcg tgtgacaaag ctaatgaggc tggtgataaatggccttgga gtgtgtattc 1980 agtagatgac agtaggtcta aatggagccc ctgtcctgatactcccagca cacttaactt 2040 agctgtgttc ttgccctctt tgcatgtctc actcaaatctatccttacag tctcacctgc 2100 cctgagtgtt tcttgtggct ttaggagctt gacaatactgtattcctttc tctacagatc 2160 atgtttgaga ccttcaacac cccagccatg tacgtagccatccaggctgt gttgtccctg 2220 tatgcctctg gtcgtaccac tggcattgtg atggactccggagacggggt cacccacact 2280 gtgcccatct atgagggtta cgcgctccct catgccatcctgcgtctgga cctggctggc 2340 cgggacctga cagactacct catgaagatc ctgaccgagcgtggctacag cttcaccacc 2400 acagctgaga gggaaatcgt gcgtgacatt aaagagaagctgtgctatgt tgccctagac 2460 ttcgagcaag agatggccac tgccgcatcc tcttcctccctggagaagag ctatgagctg 2520 cctgacggtc aggtcatcac tatcggcaat gagcggttccgatgccccga ggctctcttc 2580 cagccttcct tcctgggtaa gttgtagtct cgtcccttctccatctaaag gtgaccaatg 2640 ctggaggcca cactgtaact ctgatctctt gcttttcctttcaggtatgg aatcctgtgg 2700 catccatgaa actacattca attccatcat gaagtgtgacgttgacatcc gtaaagacct 2760 ctatgccaac acagtgctgt ctggtggcac caccatgtacccaggcatcg ctgacaggat 2820 gcagaaggag attactgccc tggctcctag caccatgaagatcaaggtaa gcagccttag 2880 cctggaccca tagtggggtg tggtcagccc tgtagttgtagccaactctc ttggcttaag 2940 gaacaaccca gcatccagaa tgctcacaat cactgtcttgctttcttcag atcattgctc 3000 ctcctgagcg caagtactct gtgtggattg gtggctctatcctggcctca ctgtccacct 3060 tccagcagat gtggatcagc aagcaggagt acgatgagtccggcccctcc atcgtgcacc 3120 gcaaatgctt ctaggcggac tgttactgag ctgcgttttacaccctttct ttgacaaaac 3180 ctaacttgcg cagaaaaaaa aaatgagaca tttggcatggctttattgtt tttttgtttt 3240 ttgtttttgt tttttttaaa tttttttttt aaaaaggttttttttttttg ttttgttttg 3300 gcgcttttga ctcaaggatt taaaaactgg aacggtgaaggcgaccgcag ttggttggag 3360 caaacatccc ccaaagttct acaatgtggc tgaggactttgattgtacat tgttttttgt 3420 ttttggtttt tttaatagtc actccaagta tccacggcatagatggttac aggaagtccc 3480 tcaccctccc aaaagccacc cccaactcct aaggggaggatggctgcatc catgccctga 3540 gtccacaccg ggaaggtgac agcattgctt ctgtgtaaattatgtacttg caaacatttt 3600 tttaaatctt ccgccttaat acttcatttt tgtttttaatttctgaatgg tcagccattc 3660 gtggcctgcc cctttttttt gtccccccaa cttgatgtatgaaggctttg gtctccctgg 3720 gagtggtttg aggtgttgag gcgccagggc tggcctgtacactgacgtga gaccgtttta 3780 ataaaagtgc acaccttaca aacaagtttg tggctctgtggcttctactg ggtgtgggga 3840 gcaggctggg tgggtgtgna actccacgtg ggggaggggcaatttagggt gggctggcct 3900 tgcctgatag ctagtgggag ggctaaagga tcatgatctttaatgaggtc tcataaatac 3960 cccaccttcg gtcttgaggg agggtgagga gttgggaaggtatctccctg acacctggcc 4020 aagctggcct catcaaccct actttcctca gccagggggaggtccacatc ctcccacctg 4080 catagcttgc gtctagaggc 4100 96 116 PRT Musmusculus Ly6/neurotoxin homolog 96 Met Thr His Leu Leu Thr Val Phe LeuVal Ala Leu Met Gly Leu Pro 1 5 10 15 Val Ala Gln Ala Leu Glu Cys HisVal Cys Ala Tyr Asn Gly Asp Asn 20 25 30 Cys Phe Lys Pro Met Arg Cys ProAla Met Ala Thr Tyr Cys Met Thr 35 40 45 Thr Arg Thr Tyr Phe Thr Pro TyrArg Met Lys Val Arg Lys Ser Cys 50 55 60 Val Pro Ser Cys Phe Glu Thr ValTyr Asp Gly Tyr Ser Lys His Ala 65 70 75 80 Ser Ala Thr Ser Cys Cys GlnTyr Tyr Leu Cys Asn Gly Ala Gly Phe 85 90 95 Ala Thr Pro Val Thr Leu AlaLeu Val Pro Ala Leu Leu Ala Thr Phe 100 105 110 Trp Ser Leu Leu 115 973925 DNA Mus musculus Ly6/neurotoxin homolog 97 taccaacacc gcacgaagtgtgtacagatt cccagttaga cagcaggagg gacctgggag 60 cggccagggg gatgttttatctctaagaga ccaagagctc aggcagggct tctgtgccct 120 gcttcctccc tggcttgagctggatcctgg accagctgct gacctcctgt tcactctggc 180 actgccctca cgtctccgtcatgacccatc tgctcacagt gttcctggtg gccctgatgg 240 gcctgcctgt ggcccaggctctggagtgcc acgtgtgtgc ctacaatgga gacaactgct 300 tcaaacccat gcgctgcccagccatggcca cctactgtat gaccacacga acttacttca 360 ccccataccg gatgaaggtgaggaagtcct gtgtccccag ctgctttgaa accgtgtacg 420 atggctattc caagcatgcatctgccacct cctgttgcca gtactacctc tgcaacggtg 480 ctggctttgc taccccggtgaccttggccc tggtcccagc actcctagct accttctgga 540 gcttgctgta aagctcggttccccaagcca gatccactca aacgcaacac tctcaaaaaa 600 cacagtttcc ctctctctcccaattcactc cacccaacgc tcttccttct gacactcctc 660 aactaccacg aggtcccatggctacctacg aaagaactga tggcatccag atacctcact 720 ccaaggtcat tttcagaaggctgacatgtg gacctgtaat gtgcccaccc atgggtgggg 780 caggctgggc ttctcctctacccaagatca ggggcatctg ggagaatgtt tatggaggag 840 gggtcatcac tcaagtcaaggagcactgat ttgatagaat tagtagccaa actccacctt 900 cagaaccctg cctcagtctacccagtagag gatgggtctg ctagaggtga ggggaggaga 960 gcggcggaga ataacgagctggctagaagc agagaaagac tcagcagggc tgtctccgaa 1020 gatcagcgcg gcttgccagagcaaatgtga tgtggaagcc atgtgaggaa gccctttgtc 1080 atttccactt atctgaggaactctgccaga cctgatgttg ggatagccat tggccaaggg 1140 ttcctagcaa cggcgtcatttccataggcc actgaaatcc ctccagcccc agctcagcag 1200 gccccttgac ctccactacagtccttcatt cacacaccag ctgctgggcc ttgaagttgg 1260 cagggacttg ggagcaggtgacccatgcta ttttttgtct ggcctgttat tctgggcatg 1320 gcaagaaggg atcagacgcaggtcagagca gggcagtagg gcgactgaga cagggaaaca 1380 gacttcagcc agtggcttcccaggtcccgt aggcagctcc tacatccttc agtctcttgt 1440 tacattcccg ggagacaaatatacagggag ccaagccgag tgctaggtga tgactgcctg 1500 tgaagtctat tgtggccacagactgctggg taccaagtct caggagaacc cagcctagat 1560 ttaggagaca cagatctgcctttcatgcag tgtagctgtc cttgggagcc ttaccatgct 1620 ctctaactag ttcctcaactcacatgtcac tgaggaaccc cctaacactg gcccagccca 1680 ggggtcggga tgctggccaatgtccatgga gtgggactac ccctggagag tccttgggtc 1740 atcacatcac aaatgttttattccaacctc ccagtggtga gagctcggga cacaaaggtc 1800 catcctgggg accttcttcctggttctagg cagacctgaa ctctgtctgc tgctagagct 1860 gatgtggttt tccgcctcagtttcctcctc cggggatagg ccaccggagg atttgggagg 1920 gtggggaggg catcctgctgatgggctcgc cgaggttctc aggaacagga acgggcgggg 1980 ctttagtaca caggtgagttgggtgggaac tggcccggag ctgaggagac actgactggg 2040 cagagggaag atgagtctcaagggagggca ggaaaaggga gggggagcgc gcatgcacat 2100 gtgcactcag tgcaggctacagagcccaaa aggcagcact ggctgtggtg tcccctgagg 2160 cccaggcaag atgctaggaggaagccaatg ctgcccccac ctgagctcac atggaacatg 2220 cacaccacca gcagcagcagcaagcattga gactgacctg tggacgccat agggcactgg 2280 caaggagggt cagaggcgggtccctgactc agtgggtgag gcccgggaaa cattatcctg 2340 ttaccctgcg tgtgcaagatcattgtcccc agctagatgg cgtcctcaac caaaactgag 2400 aggagcccca gttcaggtcctccctcctac cacaaggggg tggtgtggag gaggcttgat 2460 tgcccttgga gaagcaccggtactgcagag ctgggggcca gcttctttca tctgtgtcta 2520 gacaccgacc agataggccccacagtggca acactgccac acagtcctac aagaagccct 2580 gtgcctagct agcacagagccccaaaaggt gctcaattaa tacagggcca agcctgccag 2640 tgggggggat gcagattaggggaacagacc cagatggcct gtcctgaacc ctgtctgggg 2700 tggtgtgatg agcatctgtctagcccactg caggtggctc tacacactcc acaacagttc 2760 tgcaaaagtg tatgaggtggtcattactgc gcccctctca caggtaaagg cactgaggca 2820 cggaggagtg aggcacttcattttcctggg ccattcaact ttccaggacc aacacattca 2880 actatgggta ctactccaatagctggggtt ctttgaggct gggccccctg aagatgatag 2940 tggcttcatc aaccagagaatttcagagtg cagtgttgta ggagcctatg aacctgaaat 3000 gtcagaactg gaggtttgaggggctgaggg gtaggccagg ggtgtctggc cccttgtgtg 3060 gagacagaga gagagggaacatgggatggg gtagtagaga gaagtgcaaa ggagcgtcag 3120 cctttctcag ggctaatgctgtcagggacg agggctcaag cctgtgagtg ttctcacact 3180 gtgataaaca gtggcccctcaacacagacg gtgtccagag tggccggcag tggttatcta 3240 gagttgcaat ctggaagcctcttggtagtc actggagaga ggccgcttga tgggacagca 3300 ccaaatgtgt gtgcttctgtgggatgtgag gaagctgggt cagcgcatga agccaaagcg 3360 tccttcagag cagaggggtggctggtctag tccaccagag acaagctatc cagtgagagt 3420 catactctgt caccgtctctgtgattacct taccccaaag cagacgggga cgggatgcag 3480 agcacccgtg tcttcatcttctgcggcaag cacgtgagtt cacattctga aactctagaa 3540 agatttccag gagtggggtgtgcctttgct ttggtgcatg gttacttcct ggcaagcacc 3600 gtggcatccc gcagcactgagtgacctggg ctcctcaagc catctcattg gtgaaatgac 3660 agtgccagta ccctctcagctggctcttgg aggcctgtgc atggggtctg cacagaggag 3720 gcccccaaac tatgcatggacggacacgtg atgcctagca cttcccttgg ttgtgtctct 3780 gccaacccca ggctctcacccagcaaggaa atgaaatcca cttttatgac acatctccct 3840 cccccagcca gctccattcacctatatgcc agggtggtcc ctttcaatgt ctgtccccca 3900 ttggatgaat aaacaagcgaaggac 3925 98 130 PRT Rattus norvegicus Astrocytic phosphoprotein 98 MetAla Glu Tyr Gly Thr Leu Leu Gln Asp Leu Thr Asn Asn Ile Thr 1 5 10 15Leu Glu Asp Leu Glu Gln Leu Lys Ser Ala Cys Lys Glu Asp Ile Pro 20 25 30Ser Glu Lys Ser Glu Glu Ile Thr Thr Gly Ser Ala Trp Phe Ser Phe 35 40 45Leu Glu Ser His Asn Lys Leu Asp Lys Asp Asn Leu Ser Tyr Ile Glu 50 55 60His Ile Phe Glu Ile Ser Arg Arg Pro Asp Leu Leu Thr Met Val Val 65 70 7580 Asp Tyr Arg Thr Arg Val Leu Lys Ile Ser Glu Glu Asp Glu Leu Asp 85 9095 Thr Lys Leu Thr Arg Ile Pro Ser Ala Lys Lys Tyr Lys Asp Ile Ile 100105 110 Arg Gln Pro Ser Glu Glu Glu Ile Ile Lys Leu Ala Pro Pro Pro Lys115 120 125 Lys Ala 130 99 2341 DNA Rattus norvegicus Astrocyticphosphoprotein 99 gcggaagagg cggcggcggc aggatcggcg gcagcggtgg ccggagccaaggagcaggct 60 ccagacgctg cttaggaacc ggggatccgg gagtgcccgc accctgagctctcagctccg 120 gaggcgtcat ggcagagtac gggactctcc ttcaggacct gaccaacaacatcacccttg 180 aagatctgga acagctcaag tcagcctgca aggaggacat ccccagtgagaagagtgaag 240 agatcactac aggcagtgcc tggtttagct tcctggagag ccacaacaagctggacaaag 300 acaacctctc ctacattgag cacatctttg agatctcccg ccgtcctgacctactcacga 360 tggtggttga ctacagaacc cgtgtgctga agatctctga ggaggatgagctggacacca 420 agctaacccg tatccccagc gccaagaagt acaaagacat tatccggcagccctctgagg 480 aagaaatcat caaattggct cccccaccaa agaaggcctg agcaagggggaggaagagga 540 ggaaggttgg atcttcatca gaccactccc ttccccatcc tcaatgggaggggctagggc 600 aacaccctgc tccgtaccca cttactaact tggtcctaac ccttactatgcgcgtgtgtg 660 tgcgtgtgcg cacgctctgg ctgtctgtct gtctagctca tctagttcctcctcctcatg 720 aggggattgg agtcaaggga ggggggctta gctgccccca ctgtaaggggaggtggacgc 780 tttttctatg taaacagaaa ctggcacact cctcctcctc tcctctcctccactcttccc 840 cacatcttta aagtatggaa gcagaaaggg cctgcgtttt cccacactgaggagctatgg 900 tgaaaatgag gtatggcgga aatgattaga tctaaagaaa accagtggggaaggaaggtc 960 aagaggctac ccaacctcca cctgaagggg ctcctgagtg ccagcgggccctcctttatg 1020 cccttgttac gggcctggga tgtggataag cagagatcct aatgagaagaccactagctc 1080 ctttcctagg tcccaagatc aaaccccagt ggagaagcca gcttttgatgtccccaactt 1140 ctgctctgga gagactatgc tgggaccatg cactgctgag cctgagccagggatgcaggc 1200 agagacaggc ccactctcgc tcctcagttt ctagatacag actgttgggatatactgacc 1260 cggagcctag gaattgggga atgagatccc ttaggtttta accgcaacctgcagagtccc 1320 aaaatgggta ttgtaggcaa cctttccact ttccagttcg gaattctccaagcaagagat 1380 aagttaacga cagtgtttcc tttgcactga ctaccacccc attcaatccaggaagggact 1440 ggtcaccttt ctcatttggg tttgtgaatg ccacacagcc aagccactagagtacagtga 1500 gtgaacccag cctcctccct gtcccaagat gcccttcccc atcttgaccgtgctaactgt 1560 gtgtacatat atattctaca tatatgtata ttaaacccgc actgccatgtctgccctttt 1620 ttgtggtttt tagcattaac ttattgtcta ggccagagcg ggagtgggaggggaatgcca 1680 cagtaaaggg agtggcagag ccaaattgct aagttagtca aaacagaaaacatttccaac 1740 caatcacaac aaacattgca tttacatttg agctaagaga caatttagagaaagttgaag 1800 gtaggagctt ttaggatgtg ggagcaaaac ttcacaggga agggaggaccagctgtcaga 1860 agagggctct tgaagccaga ctggctagag caccctcact tcctagctgagcccagtttg 1920 cccatctcct ctggccactg ctgcagacac ctgccttaac acacacacctctaagactcc 1980 agttttgcct taaaggccct tcctaagtct ccctagtcct gcctggcatctcctttgaga 2040 caagaaatcc ttgtacaact gggagaaagg aactatgggc aacatccttccatctggggt 2100 gttacgtgag agcaggagtt aagacggagg ctttccttat gaagacacaggagagagaga 2160 gggtgcttct cagcagtttc tctgtaaggc aaaaaacaca aaccttaaacacactaacct 2220 gccctaatac actcacctac cctgctaccc tcgtgctgct gtgaggttgcccctagcctg 2280 tgttcctctg tctgcagcgt gcacggcctt gttctaaccc tggaataaaggtgactgact 2340 g 2341 100 582 PRT Mus musculus PLIC-1 100 Met Ala GluSer Ala Glu Ser Gly Gly Pro Pro Gly Ala Gln Asp Ser 1 5 10 15 Ala AlaAsp Ser Gly Pro Ala Glu Pro Lys Ile Met Lys Val Thr Val 20 25 30 Lys ThrPro Lys Glu Lys Glu Glu Phe Ala Val Pro Glu Asn Ser Ser 35 40 45 Val GlnGln Phe Lys Glu Glu Ile Ser Lys Arg Phe Lys Ser His Ile 50 55 60 Asp GlnLeu Val Leu Ile Phe Ala Gly Lys Ile Leu Lys Asp Gln Asp 65 70 75 80 ThrLeu Ser Gln His Gly Ile His Asp Gly Leu Thr Val His Leu Val 85 90 95 IleLys Thr Gln Asn Arg Pro Gln Asp Asn Ser Ala Gln Gln Thr Asn 100 105 110Thr Thr Gly Asn Ser Val Thr Ser Ser Pro Ala Pro Asp Ser Asn Pro 115 120125 Thr Ser Gly Pro Ala Ala Asn Ser Ser Phe Gly Leu Gly Gly Leu Gly 130135 140 Gly Leu Ala Gly Leu Ser Ser Leu Gly Leu Asn Thr Thr Asn Phe Phe145 150 155 160 Glu Leu Gln Ser Gln Met Gln Arg Gln Leu Leu Ser Asn ProGlu Met 165 170 175 Met Val Gln Ile Met Glu Asn Pro Phe Val Gln Ser MetLeu Ser Asn 180 185 190 Pro Asp Leu Met Arg Gln Leu Ile Met Ala Asn ProGln Met Gln Gln 195 200 205 Leu Ile Gln Arg Asp Pro Glu Ile Ser His MetLeu Asn Asn Pro Asp 210 215 220 Ile Met Gly Gln Thr Leu Glu Leu Ala ArgAsn Pro Ala Met Met Gln 225 230 235 240 Glu Met Met Arg Asn Gln Asp ArgAla Leu Ser Asn Val Glu Ser Ile 245 250 255 Arg Gly Gly Tyr Asn Ala LeuArg Arg Met Tyr Thr Asp Ile Gln Glu 260 265 270 Pro Met Ile Asn Ala AlaGln Glu Gln Phe Gly Gly Asn Pro Phe Gly 275 280 285 Ser Leu Val Ser SerPro Ser Ser Ala Glu Gly Thr Gln Pro Ser Arg 290 295 300 Thr Glu Asn ArgAsp Pro Leu Pro Asn Pro Trp Ala Pro Gln Thr Pro 305 310 315 320 Gln SerSer Pro Ala Tyr Gly Ser Ser Gly Ser Thr Asn Asn Thr Val 325 330 335 SerThr Ser Gly Gly Asn Ala Thr Ser Thr Pro Ala Gly Gln Gly Thr 340 345 350Ser Gly Pro Lys Leu Val Pro Gly Ala Gly Gly Ser Met Phe Asn Thr 355 360365 Pro Gly Met Gln Ser Leu Leu Gln Gln Ile Thr Glu Asn Pro Gln Leu 370375 380 Met Gln Asn Met Leu Ser Ala Pro Tyr Met Arg Ser Met Leu Gln Ser385 390 395 400 Leu Ser Gln Asn Pro Asp Leu Ala Ala Gln Met Met Leu AsnAsp Pro 405 410 415 Leu Phe Ala Gly Asn Pro Gln Leu Gln Glu Gln Met ArgGln Gln Leu 420 425 430 Pro Thr Phe Leu Gln Gln Met Gln Asn Pro Asp ThrLeu Ser Ala Met 435 440 445 Ser Asn Pro Arg Thr Met Gln Ala Leu Leu GlnIle Gln Gln Gly Leu 450 455 460 Gln Thr Leu Ala Thr Glu Ala Pro Gly LeuIle Pro Gly Phe Thr Pro 465 470 475 480 Gly Leu Ala Ala Gly Asn Ser GlyGly Pro Ala Gly Thr Thr Ala Pro 485 490 495 Ser Thr Ala Pro Gly Glu AspThr Asn Pro Gln Gly Gly Ala Ala Glu 500 505 510 Pro Gly His Gln Gln PheIle Gln Gln Met Leu Gln Ala Leu Ala Gly 515 520 525 Val Asn Pro Gln LeuGln Ser Pro Glu Val Arg Phe Gln Gln Gln Leu 530 535 540 Glu Gln Leu SerAla Met Gly Phe Leu Lys Arg Glu Pro Asn Leu Gln 545 550 555 560 Ala LeuIle Ala Thr Gly Gly Asp Ile Asn Ala Ala Ile Glu Arg Leu 565 570 575 LeuGly Ser Gln Pro Ser 580 101 1843 DNA Mus musculus PLIC-1 101 ccatggccgagagcgcagag agcggcggcc caccgggcgc tcaggacagt gcggccgaca 60 gcggccccgcagagcccaag atcatgaagg tcacggtgaa gacgcctaaa gagaaggaag 120 agttcgctgtgcccgagaac agctcggtcc agcagttcaa agaggaaatc tcaaaacgtt 180 tcaaatctcatattgaccaa cttgtgttga tatttgctgg aaaaatttta aaagatcaag 240 atactttgagtcagcatggg attcatgatg gacttacggt tcaccttgtc atcaaaaccc 300 aaaacaggccgcaagataat tcagctcagc aaacaaatac cactggaaac agtgtgacca 360 gttcaccagctcctgacagt aaccccacgt ctgggcctgc tgctaacagc tcctttggtt 420 taggtggacttggaggactt gcaggtctta gtagcttggg tttaaatacc accaacttct 480 ttgaactacagagccaaatg cagcggcaac ttttgtctaa ccctgaaatg atggtccaga 540 tcatggaaaacccctttgtg cagagcatgc tctcaaaccc tgacctgatg cggcagctga 600 tcatggccaacccacagatg cagcagctga tacagagaga cccagagatc agtcatatgc 660 ttaataacccagatataatg ggacagacat tggaacttgc cagaaaccca gcgatgatgc 720 aggaaatgatgagaaaccag gaccgagcct tgagcaacgt agaaagtatc cgtggagggt 780 acaacgccttacggcgcatg tacacagata ttcaggagcc catgataaac gctgcgcaag 840 agcagtttggtggtaatcca tttggttcct tagtgagcag tccgtcctca gcagaaggga 900 ctcagccttctcgaacagaa aatagggatc cgctacctaa cccttgggct ccacagactc 960 cccagagctctccagcctat ggcagcagtg gcagcaccaa taacacagtg agcacctctg 1020 gtggcaatgccaccagtacc ccggcgggac agggcacctc agggcccaag cttgtgcctg 1080 gagcaggaggtagtatgttc aacacccccg gaatgcagag cctgctgcag cagataactg 1140 agaacccacagctcatgcag aacatgctgt ctgccccata catgaggagc atgctgcagt 1200 ccctgagccagaaccccgac cttgctgcgc agatgatgct gaatgacccc ctatttgctg 1260 ggaaccctcagcttcaagag caaatgagac aacagctccc aacgttcctc caacaaatgc 1320 agaaccccgacacattgtca gccatgtcga accctagaac catgcaggcc ctgctgcaaa 1380 tccagcagggcttgcagacg ttggccacag aagctcccgg cctcatccca gggtttactc 1440 ctggcttggcagcgggcaat tctggaggcc ctgcaggaac cactgcacct agcactgcac 1500 ctggtgaggatacgaacccc caagggggcg ctgctgagcc aggccaccag cagtttatcc 1560 agcaaatgctgcaggccctc gccggggtga accctcagct gcagagtcca gaagtcagat 1620 ttcaacagcaactggaacag ctcagtgcaa tgggattctt gaagcgtgaa ccaaacttac 1680 aagcactgatagcaacaggg ggcgacatca atgcggccat tgaaaggttg ctgggttccc 1740 agccgtcatagcagcatttc tgtatcctga aaaaatgtaa tttatttttg ataacggctc 1800 ttaaatctttaaaataacct gctttatttc attgggattc tgt 1843 102 618 PRT Rattus norvegicusNfxl 102 Met Ala Asp Glu Gly Lys Ser Tyr Asn Glu His Asp Asp Arg Val Ser1 5 10 15 Phe Pro Gln Arg Arg Lys Lys Gly Arg Gly Pro Phe Arg Trp LysCys 20 25 30 Gly Val Gly Asn Arg Arg Ser Gly Arg Gly Gly Ser Gly Ile ArgSer 35 40 45 Ser Arg Phe Glu Glu Asp Asp Gly Asp Val Ala Met Asn Asp ProGln 50 55 60 Asp Gly Pro Arg Val Arg Phe Asn Pro Tyr Thr Thr Arg Pro AsnArg 65 70 75 80 Arg Arg Asp Thr Trp His Asp Arg Asp Arg Ile His Val ThrVal Arg 85 90 95 Arg Asp Arg Ala Pro Gln Glu Arg Gly Gly Ala Gly Thr SerGln Asp 100 105 110 Gly Thr Thr Lys Asn Trp Phe Lys Ile Thr Ile Pro TyrGly Lys Lys 115 120 125 Tyr Asp Lys Met Trp Leu Leu Ser Met Ile Gln SerLys Cys Ser Val 130 135 140 Pro Phe Asn Pro Ile Glu Phe His Tyr Glu AsnThr Arg Ala His Phe 145 150 155 160 Phe Val Glu Asn Ala Thr Thr Ala SerAla Leu Lys Ala Val Asn Tyr 165 170 175 Lys Ile Gln Asp Arg Glu Asn GlyArg Ile Ser Ile Ile Ile Asn Ser 180 185 190 Ser Ala Pro Pro Tyr Ile ValGln Asn Glu Leu Lys Pro Glu Gln Val 195 200 205 Glu Gln Leu Lys Leu IleMet Ser Lys Arg Tyr Asp Gly Ser Gln Gln 210 215 220 Ala Leu Asp Leu LysGly Leu Arg Ser Asp Pro Asp Leu Val Ala Gln 225 230 235 240 Asn Ile AspVal Val Leu Asn Arg Arg Gly Cys Met Ala Ala Ala Leu 245 250 255 Arg IleIle Glu Glu Asn Ile Pro Glu Leu Leu Ser Leu Asn Leu Ser 260 265 270 AsnAsn Arg Leu Tyr Lys Leu Asp Asp Met Ser Ser Ile Val Gln Lys 275 280 285Ala Pro Asn Leu Lys Ile Leu Asn Leu Ser Gly Asn Glu Leu Lys Ser 290 295300 Glu Trp Glu Leu Asp Lys Ile Lys Gly Leu Lys Leu Glu Glu Leu Trp 305310 315 320 Leu Asp Arg Asn Pro Met Cys Asp Thr Phe Leu Asp Gln Ser ThrTyr 325 330 335 Ile Ser Thr Ile Arg Glu Arg Phe Pro Lys Leu Leu Arg LeuAsp Gly 340 345 350 His Glu Leu Pro Pro Pro Ile Ala Phe Asp Val Glu AlaPro Thr Met 355 360 365 Leu Pro Pro Cys Lys Gly Ser Tyr Phe Gly Thr GluAsn Leu Lys Ser 370 375 380 Leu Val Leu His Phe Leu Gln Gln Tyr Tyr AlaIle Tyr Asp Ser Gly 385 390 395 400 Asp Arg Gln Gly Leu Leu Asp Ala TyrHis Asp Gly Ala Cys Cys Ser 405 410 415 Leu Ser Thr Pro Ser Asn Pro GlnAsn Pro Val Arg His Asn Leu Ala 420 425 430 Lys Tyr Phe Asn Asp Ser ArgAsn Val Lys Lys Ile Lys Asp Thr Thr 435 440 445 Thr Arg Phe Arg Leu LeuLys His Thr Arg Leu Asn Val Val Ala Phe 450 455 460 Leu Asn Glu Leu ProLys Thr His His Asp Val Asn Ser Phe Val Val 465 470 475 480 Asp Ile SerAla Gln Thr Ser Thr Leu Leu Cys Phe Ser Val Asn Gly 485 490 495 Val PheLys Glu Val Asp Gly Lys Ser Arg Asp Ser Leu Arg Ala Phe 500 505 510 ThrArg Thr Phe Ile Ala Val Pro Ala Ser Asn Ser Gly Leu Cys Ile 515 520 525Val Asn Asp Glu Leu Phe Val Arg Asn Ala Ser Pro Glu Glu Ile Gln 530 535540 Arg Ala Phe Ala Met Pro Ala Pro Thr Pro Ser Ser Ser Pro Val Pro 545550 555 560 Thr Leu Ser Gln Glu Gln Gln Asp Met Leu Gln Ala Phe Ser ThrGln 565 570 575 Ser Gly Met Asn Leu Glu Trp Ser Gln Lys Cys Leu Gln AspAsn Asn 580 585 590 Trp Asp Tyr Thr Arg Ser Ala Gln Ala Phe Thr His LeuLys Ala Lys 595 600 605 Gly Glu Ile Pro Glu Val Ala Phe Met Lys 610 615103 2304 DNA Rattus norvegicus Nfxl 103 attccatcct aatacgactc actatagggctcgagcggcc gcccgggcag gtccaaggcg 60 tccgggtgtt tggtggcgtc aggttctgcgcctgagcccg cccctgtacc tgcctgtgcc 120 atggcggacg aagggaagtc atacaacgaacatgatgacc gtgttagttt cccacaaaga 180 agaaagaaag gtcgggggcc tttccgatggaagtgtggtg tggggaatcg taggtctgga 240 agaggtggtt ctggtatacg gtcttcccggtttgaggaag atgatggaga tgtggcaatg 300 aatgatcccc aggatggccc ccgagtgagattcaacccct ataccacccg acctaaccgt 360 cggagagata cttggcatga tcgagatcggattcatgtta ctgtacggag agacagagct 420 cctcaagaaa gaggaggtgc tggcaccagtcaagatggga ccaccaagaa ctggttcaag 480 attacaattc cttatggcaa aaagtatgataaaatgtggc ttctgagcat gattcaaagc 540 aagtgcagtg tccccttcaa ccccattgagtttcactatg aaaatacacg ggcccatttt 600 tttgtggaaa atgccactac cgcttctgcattaaaggctg tcaactataa gattcaggat 660 cgagaaaacg gaaggatatc tatcatcatcaactcttctg ctccacccta tattgtacag 720 aatgaactga agcctgaaca agtagagcagctcaagctga tcatgagcaa acgatatgat 780 ggctcccagc aagcacttga cctcaagggcctccgctcag acccagattt ggtggcccag 840 aacatcgatg ttgttctaaa ccgtagaggctgtatggcag ccgccctgag gatcattgaa 900 gagaacattc ctgagctatt gtctttgaacttgagtaaca acaggctgta caagcttgat 960 gacatgtcca gcattgtaca gaaggcaccaaacttaaaga tcctgaatct ctctggaaac 1020 gaattgaagt ccgagtggga attagacaagataaaagggc tgaagctgga agagctgtgg 1080 cttgacagaa accccatgtg tgacaccttcctagaccagt ccacctatat cagcaccatt 1140 cgcgaacgat ttcccaagtt attacgcctggatggccatg agctaccccc tccaattgcc 1200 tttgatgtcg aagcccctac tatgttaccaccctgcaagg gaagctattt tggaacagag 1260 aacctaaaga gtctggtcct gcacttcttgcagcagtact atgcaattta tgactcagga 1320 gaccgccagg gtcttctgga tgcctaccatgatggcgcct gttgctcgct aagcactcct 1380 tccaaccctc agaaccctgt ccgacacaacttagccaaat acttcaatga cagcagaaat 1440 gtgaagaaga ttaaagatac caccacgcggttccggctat taaagcatac acgtcttaat 1500 gtcgttgcct tcctcaatga gttacctaaaactcaccatg acgttaactc ctttgtggta 1560 gatataagtg cccaaacaag cacattgttgtgtttttctg tcaatggagt cttcaaggaa 1620 gtggacggga agtctcgaga ttctttacgtgctttcacga gaacgttcat tgctgttcct 1680 gccagcaatt cagggctatg tattgtaaatgacgaattat ttgtgcggaa cgccagccct 1740 gaagaaatcc aaagagcctt cgccatgcctgcacccacac cttcttccag cccagtaccc 1800 acactctccc aagaacagca ggatatgttacaagcatttt ctacccagtc tggcatgaac 1860 ctcgagtggt ctcagaagtg ccttcaagacaataactggg actacaccag atctgcccag 1920 gccttcactc atctcaaggc caagggagagattccagaag tagcgtttat gaaatgatct 1980 caagaaaagt ccccttgtaa atagcccttggataatattg tctggctgtc atctgcggac 2040 tcctgtccag cccgaggcca cmctgtaactgtgactgggg aggaagggct gcytcatcck 2100 tcctcaccta ccttctggaa gmcttagcagaagacttccg gaagattgag cctctatggt 2160 gccaggaagc caaagcttac tttgtagagctdgacrctaa attaccggaa ggacttaggt 2220 gctctgtgta cttaacccca gsattccctttactttttaa gataaagagt gatattgtag 2280 tgtgtgtccc aaaaaaaaaa aaaa 2304104 175 PRT Rattus norvegicus Alpha B-crystallin 104 Met Asp Ile Ala IleHis His Pro Trp Ile Arg Arg Pro Phe Phe Pro 1 5 10 15 Phe His Ser ProSer Arg Leu Phe Asp Gln Phe Phe Gly Glu His Leu 20 25 30 Leu Glu Ser AspLeu Phe Ser Thr Ala Thr Ser Leu Ser Pro Phe Tyr 35 40 45 Leu Arg Pro ProSer Phe Leu Arg Ala Pro Ser Trp Ile Asp Thr Gly 50 55 60 Leu Ser Glu MetArg Met Glu Lys Asp Arg Phe Ser Val Asn Leu Asp 65 70 75 80 Val Lys HisPhe Ser Pro Glu Glu Leu Lys Val Lys Val Leu Gly Asp 85 90 95 Val Ile GluVal His Gly Lys His Glu Glu Arg Gln Asp Glu His Gly 100 105 110 Phe IleSer Arg Glu Phe His Arg Lys Tyr Arg Ile Pro Ala Asp Val 115 120 125 AspPro Leu Thr Ile Thr Ser Ser Leu Ser Ser Asp Gly Val Leu Thr 130 135 140Val Asn Gly Pro Arg Lys Gln Ala Ser Gly Pro Glu Arg Thr Ile Pro 145 150155 160 Ile Thr Arg Glu Glu Lys Pro Ala Val Thr Ala Ala Pro Lys Lys 165170 175 105 6806 DNA Rattus norvegicus Alpha B-crystallin 105 gggaagagcagcctatgact ttctgcccac acggacgcca ttcggtctac caggttacag 60 acaggagcagcacaaacaag gcccctagat tttggtttat ttagcctcac ccagaattaa 120 atcgaaggctacgattcatc aagggaccaa accatacact gtggaccatg cacctatccc 180 atctcccaggaatgctgctc cctctggggt gtggtggatt tctggaatca ccacgggagg 240 tagacaggggattcaccggg ttctgggtct ggcagtcagg gttcaactct ggctacctct 300 tcctcttcctcggggtcagg aggtgcagga agcagggaga tgtagacctc attgacttcc 360 gtgtccagatgccggccacc ccgcggtgcc tccaagttaa ggatgccatc atgggagaga 420 gcagctctgacccgccaggg gtccacatct gcaggcagga cataggttgc gacagaactc 480 tcgggacacgaagccgtggc gtccagacgt tgggggtgtc gggcagacac tctagcaggt 540 tgtccacagtcctcacggtc acctcatctg gggtaaagtg gctcacatcc agaaatgcct 600 ggaacttgccttcactgagc ctgagctctg aggcccctgc cctgccgccc tccccagctc 660 ctggcggcccggggccgaac atagtagcca tggtagaggg tgggggtcag gatctcttct 720 ggcaggaggcctgggagcag agaaagaaag agaaggcaag aggcaggatg aggaagaatg 780 cagcgggcacagacagggct gaaaggcgat atctggagat atgggctaga agagcaggtg 840 ggaaactgtagaggggctgg gaaaggagag gagatggtta cggaaaaagc gagctggaac 900 tggaggacagaagcaaaacc agaaaatact ctgaagcgtg caaggcccgg acaccgaaag 960 ccctgccgatctaaagtttg gtcctgaagt ctgttagcaa gaccagagag agtcttgctt 1020 gaggggggagtctggggtgg gagatggctc acctcaggag ttcacttaag ccccaggtag 1080 agtggaggccagtgaagctg ggcagtgagc ccagaatttc cggaggtggg ggtaggaagc 1140 atctgagccataccttctcc gaagcgctgc tcgccccagg cggctagggt tggcaaattc 1200 gtactcggcagtggctgggt gggcgtgtgg cactgtgcgg cccgacatga ctgctgttgc 1260 gactagtagccctgatccca agtgtagccc caacaagatc aaggcgaccc ctccagcttc 1320 agagggagagccaggactca gtcagagtgg gggcggggtc tacaccaccc aaaatagtgc 1380 agagcgtctgggggatgggg gaggggtggg agcctaagtc tagagtgcag ctatgagggt 1440 gtgatcagcgctgcctggac cccctaagtc ccctcctctg tatacccttc agctgtcgca 1500 taggcctggaagaacagctg agggtcctgg tcctagagta agctgggaag agaaactggt 1560 gcgctgcaggagggcaagga gaggcctggt tgggcccttc accaattgta cattccacat 1620 caccctttgtccttatcagt ctcaggcact gagcacatta cttagaaaaa aaaaaaaaaa 1680 gacaatgttctatgagccac agaacgtcaa aaatacaaga aacattttct gtctttttaa 1740 tgtcagggtcttctgaacct agatcaactc ggggttccag tcagacacct agttctgaca 1800 tattggtggtcacagctctc ctctgggact ccacaaagag ttaatgtccc tgggctcagc 1860 ccaggaagattccagcctct gcccaggccc aagatagttg ctggctcaat tcccctggca 1920 tgcaagactggagaggagga ggggcccacc agcagctgct tgggattcca gaccctgtcc 1980 tggctccagagaacaaggat ggggtgggtg ggtgccacta ggtgtggaca gagagctagt 2040 gaaacaagaccgtgacaagt caccggccag ctcagccctg ccccgtgttt ctcttttctt 2100 agctcagtgagtactgggta tgtgtcaccc tgccaaatcc ctgatcacaa gtccccatga 2160 actgtcggggagctgggata ataaaacccc tgacatcacc gttccagaag cttcacaaga 2220 ctgcatatataaggggcagg ctgtagcagc ggctgaagga gttgaccggc taaccgactc 2280 tacactcatctagccatcat ggacatagcc atccaccacc cctggatccg gcgtcccttc 2340 tttcctttccactccccaag ccgcctcttt gaccagttct tcggagagca cctgttggag 2400 tctgacctcttctctacagc cacttccctg agccccttct accttcggcc accctccttc 2460 ctgcgggcacctagctggat tgacactggg ctctcagagg taagtctccc ttgtgccagg 2520 gcaggaatacgtatccttcc tggtctctcc attcctttgc cctttcaccc tgcctaagtc 2580 attttaggcatgtgcgtgtg caaaggtaca gagaaatgag gttactgttc tcttcctgtg 2640 gcacctgttctgttgatgct cctgttcctg gtccattttc tgtgcatggt gcctctttct 2700 cctctttcaactcctttacc cacctcttta atagtcccag tcctggcaga ttttatcatc 2760 tcaaactgtagagatcagat agaaacccac tcattctggg cttttcctgt cttctttcct 2820 ccccacttccttttgatgga gctggataca tcaatcaata ttctctctct ctctctctct 2880 ctctctctctctctctctct ctctctcttc cttggcctat gactttccgg gttcctcggg 2940 gctgagacagtgcattgact tcagtccatg ccttaagagg accgctcttg ggaagccctg 3000 acttcttatctcagcagcgt gggagataag attaacagag gttgtcacag ataacactgt 3060 ggtttaaaaatattcgcgtg tgaggaaaca ggagctgagt gagcaagggc tttggaagga 3120 cagaacattccagatcgagt gggttggaaa cttggcaggc aactgaacaa gaaaaagggg 3180 gcttttgttttatagacaaa tgacaaagcc agcactgggt agagaggcag cagatgccat 3240 ccccagacccattcttgtga ctagtctcct actgacccgt cttcttccct gtgcctctcg 3300 gtaaagtttgggactgatgg atgactaaga ggtgtctttt gggtatcacc atggtggctc 3360 acatataccagtgtcactag tcaataagag gatttttccc ccagcagcca ggtagaggat 3420 ggattaccagggcaggaagt aaatctcctg gacgatgagc cttctccagc ttaatggaca 3480 ttctaccatcttttctcctc cgcccccttg ttattctaga tgcgtatgga gaaggacagg 3540 ttctctgtgaacctggacgt gaagcacttc tctccagagg aactcaaagt caaggttctg 3600 ggagacgtgattgaggtgca cggcaagcac gaagagcgcc aggtgtgtgg acctttctgc 3660 cttctcttgtgaattcattt agtgccctgc tggatgccag ggactgccgc caccagcctc 3720 tgaggctggcagaattccag tccccaaaca taagactaga tcaggagtta tagatcaatg 3780 gctgggaaacaagttagggc tagatctgct gttagtgttg tggctgtgct gagagaactt 3840 tcccctggatgttccttttg attgccttgt cttgtccagt gacgtcagaa tagcagaaga 3900 attctcaacattcccaaccc tcagcacatc ctaggccaat tgaatctgaa gttttaggga 3960 catagctcaggcatcaacct ttaaacaaac aaacaaacaa actcactcct tgggtacttt 4020 ctgtaccccaaactggcagc cattgctcta ggcacagtgt gctgtacata atggctctgc 4080 gaaatgacagtcaccacgga gtcgaaggca ataaaatctg tggcttttga atgttcagga 4140 ttccatgcctcttctgctca gtttaatgct atgctacttg gtttgtctgc ttttcagagc 4200 ttcaggcttatggctgtgag gaagttaatt gagatttgtt tgtgtggatg cttggggtac 4260 ttgggattcaataggtaaca ggattagcat ccacaattcc tcttgcattt ttagcatttt 4320 tttttcttgacttcctaaaa aggtgttatg attgcttctt aaaaattaat gacttggggg 4380 gctggggatttagctcagtg gtagagcgct tacctaggaa gcgcaaggtc ctgggttcgg 4440 tccccagctccgaaaaaaaa gaaccaaaaa aaaaaaaatt aatgacttgg ggttggggat 4500 ttagctcagcggtagagcgt ttgcctagca agcgcaaggc cctgggttca gtccccagct 4560 ccgaaaaaaagaaaagaaaa aaaaattaat gacttagggc ctggagagat ggctcagcag 4620 ttaaaagcactgaccgctct tccagaggtc ctgcatacaa ttcccagcaa ccacatggtg 4680 gctcacaaccatctgtaatg ggatccaatg ccctcttctg gcatgcaggt gtacatgcag 4740 atagagtgctcatacataaa attaaaaaaa tcttaacaaa aaataactca gctgtgccgt 4800 ggtggtgcatgcctttaatc ccaacatttg ggaggcagag gtagacagat ctaagttaga 4860 ggccagcttagactacagaa tgagttccag gagagcagga gctcaacaga gaaaccctac 4920 ttatctcaaacaaaacaaaa aacaaaaatc aatacctcgg ttttgctcat gatttaggac 4980 tggaccacagtgtcactgat agggtcacct tatataatat cagcaatatt atactagaaa 5040 cattctgagacattcctgag caattagaac aatttatggg cttcctataa gatacagaca 5100 tactcggggttggggatttg gctcagtggt agagcgtttg cctaggaagc gcaaggtcct 5160 gggttcggtccccagccctg aaaaaaagaa aaaagaaaaa aaaaaaagat acagacatac 5220 tcaatgccaaagtgggagtc taaattccat ggtccagtca acatggattc attcctttgt 5280 ggaaatgccctgaattgtag ttgtgtctcc gaatatgaag atctatgaaa ttcggggctg 5340 tttcagatttcctacactct gattggtagg tgtgtccatc tggacagttt attctagcct 5400 taaacctggacaaacagctc tggacttagc tatctgcata tttacagtgt tggaggtgaa 5460 ctcaggcctttcactactag actgaatccc cagctccaga tattttttcc ccccttaggg 5520 ccttttgttctaacccaagg gcaggggatg tctagagtct ggggcaagtg gtggtatttc 5580 ctgctcttatttctctccct tgcccttcat tctctcggat taggacgaac atggcttcat 5640 ctccagggagttccacagga agtaccggat cccagccgac gtggatcctc tcaccattac 5700 ttcttccctgtcatcggatg gagtcctcac tgtgaatgga ccaaggaaac aggcctctgg 5760 ccctgagcgcaccattccca tcacccgtga agagaagcct gctgtcactg cagcccctaa 5820 gaagtagattccctttcctc gttgcatttt ttaagacaag gaagtttccc atcagcgaat 5880 gaacatctgtgactagtgcc gaagcttact aatgctaagg gctggcccag attattaagc 5940 taataaaaaatatcgttcag caacagacct gcctcgtctt tgcaaaccca gtgtgcttta 6000 aaccaatctgcaaacatagc agatctgctg atccccaccc tgtgattcag ggggctccga 6060 ggatacgtttggtcagagtg attggctcca gaactttgta cagtacaaac ccagggaaaa 6120 ccattcactctcctggtatt ccagtcttca gtttataaca cactgtactg gttaggacag 6180 agtcttaggagtagactaag aagggagaaa tggtgacgtc ccagcagggt catgatgaat 6240 cagctctggctaactccaca taaaagactt cagtgcattt ctgaagatta attacttcac 6300 cagggggacacggtggcctt ggtgttattt gaagagtgtc ctcagctaat tcacaggaca 6360 gtaaatagctcgtgggtggt gactgtcgaa cgagagagcc tctcgggaat ggcacagagt 6420 aatcaacagataccttttaa accgagacca ttctctacca aacatgaaaa tgacaataaa 6480 gggtccagcaaaaaaacaaa caccaaaatc agaagaacat cttcctccta aagttactaa 6540 cccctggggtggggggaagc ccacacaaaa tagaaacccc ccaaaaacct ctctccattt 6600 taatgggtctagacatagtt tctgtcagat tacactgagc cctgctaccc gatatctggc 6660 aagcctgtagcaccagttct catctctcct gtgttaactt taggttcact tgaggatgtg 6720 ccttttgaagatttttttta tttagtaatt attatttttt tataaaactt gataaaaaat 6780 agtatttccaactgtacagt cactag 6806 106 646 PRT Rattus norvegicus Heat shock cognateprotein 70 106 Met Ser Lys Gly Pro Ala Val Gly Ile Asp Leu Gly Thr ThrTyr Ser 1 5 10 15 Cys Val Gly Val Phe Gln His Gly Lys Val Glu Ile IleAla Asn Asp 20 25 30 Gln Gly Asn Arg Thr Thr Pro Ser Tyr Val Ala Phe ThrAsp Thr Glu 35 40 45 Arg Leu Ile Gly Asp Ala Ala Lys Asn Gln Val Ala MetAsn Pro Thr 50 55 60 Asn Thr Val Phe Asp Ala Lys Arg Leu Ile Gly Arg ArgPhe Asp Asp 65 70 75 80 Ala Val Val Gln Ser Asp Met Lys His Trp Pro PheMet Val Val Asn 85 90 95 Asp Ala Gly Arg Pro Lys Val Gln Val Glu Tyr LysGly Glu Thr Lys 100 105 110 Ser Phe Tyr Pro Glu Glu Val Ser Ser Met ValLeu Thr Lys Met Lys 115 120 125 Glu Ile Ala Glu Ala Tyr Leu Gly Lys ThrVal Thr Asn Ala Val Val 130 135 140 Thr Val Pro Ala Tyr Phe Asn Asp SerGln Arg Gln Ala Ala Lys Asp 145 150 155 160 Ala Gly Thr Ile Ala Gly LeuAsn Val Leu Arg Ile Ile Asn Glu Pro 165 170 175 Thr Ala Ala Ala Ile AlaTyr Gly Leu Asp Lys Lys Val Arg Ala Glu 180 185 190 Arg Asn Val Leu IlePhe Asp Leu Gly Gly Gly Thr Phe Asp Val Ser 195 200 205 Ile Leu Thr ThrGlu Asp Gly Ile Phe Glu Val Lys Ser Thr Ala Gly 210 215 220 Asp Thr HisLeu Gly Gly Glu Asp Phe Asp Asn Arg Met Val Asn His 225 230 235 240 PheIle Ala Glu Phe Lys Arg Lys His Lys Lys Asp Ile Ser Glu Asn 245 250 255Lys Arg Ala Val Arg Arg Leu Arg Thr Ala Cys Glu Arg Ala Lys Arg 260 265270 Thr Leu Ser Ser Ser Thr Gln Ala Ser Ile Glu Ile Asp Ser Leu Tyr 275280 285 Glu Gly Ile Asp Phe Tyr Thr Ser Ile Thr Arg Ala Arg Phe Glu Glu290 295 300 Leu Asn Ala Asp Leu Phe Arg Gly Thr Leu Asp Pro Val Glu LysAla 305 310 315 320 Leu Arg Asp Ala Lys Leu Asp Lys Ser Gln Ile His AspIle Val Leu 325 330 335 Val Gly Gly Ser Thr Arg Ile Pro Lys Ile Gln LysLeu Leu Gln Asp 340 345 350 Phe Phe Asn Gly Lys Glu Leu Asn Lys Ser IleAsn Pro Asp Glu Ala 355 360 365 Val Ala Tyr Gly Ala Ala Val Gln Ala AlaIle Leu Ser Gly Asp Lys 370 375 380 Ser Glu Asn Val Gln Asp Leu Leu LeuLeu Asp Val Thr Pro Leu Ser 385 390 395 400 Leu Gly Ile Glu Thr Ala GlyGly Val Met Thr Val Leu Ile Lys Arg 405 410 415 Asn Thr Thr Ile Pro ThrLys Gln Thr Gln Thr Phe Thr Thr Tyr Ser 420 425 430 Asp Asn Gln Pro GlyVal Leu Ile Gln Val Tyr Glu Gly Glu Arg Ala 435 440 445 Met Thr Lys AspAsn Asn Leu Leu Gly Lys Phe Glu Leu Thr Gly Ile 450 455 460 Pro Pro AlaPro Arg Gly Val Pro Gln Ile Glu Val Thr Phe Asp Ile 465 470 475 480 AspAla Asn Gly Ile Leu Asn Val Ser Ala Val Asp Lys Ser Thr Gly 485 490 495Lys Glu Asn Lys Ile Thr Ile Thr Asn Asp Lys Gly Arg Leu Ser Lys 500 505510 Glu Asp Ile Glu Arg Met Val Gln Glu Ala Glu Lys Tyr Lys Ala Glu 515520 525 Asp Glu Lys Gln Arg Asp Lys Val Ser Ser Lys Asn Ser Leu Glu Ser530 535 540 Tyr Ala Phe Asn Met Lys Ala Thr Val Glu Asp Glu Lys Leu GlnGly 545 550 555 560 Lys Ile Asn Asp Glu Asp Lys Gln Lys Ile Leu Asp LysCys Asn Glu 565 570 575 Ile Ile Ser Trp Leu Asp Lys Asn Gln Thr Ala GluLys Glu Glu Phe 580 585 590 Glu His Gln Gln Lys Glu Leu Glu Lys Val CysAsn Pro Ile Ile Thr 595 600 605 Lys Leu Tyr Gln Ser Ala Gly Gly Met ProGly Gly Met Pro Gly Gly 610 615 620 Phe Pro Gly Gly Gly Ala Pro Pro SerGly Gly Ala Ser Ser Gly Pro 625 630 635 640 Thr Ile Glu Glu Val Asp 645107 2242 DNA Rattus norvegicus Heat shock cognate protein 70 107atgcactttc aaagtagtga gaaaattttg atgttttcta atacatagaa atgatatcat 60taaaaggcag atgtggctca ttgaacgcag agccagctct cgggggtctc tgtgtggtct 120cgtcatcagc acagctgggc ctacacgcaa gcaaccatgt ctaagggacc tgcagttggc 180attgatcttg gcaccaccta ctcctgtgtg ggtgtcttcc agcatggaaa ggtggaaata 240attgccaatg accagggtaa ccgcaccacg ccgagctatg ttgctttcac cgacacagaa 300cgattaattg gggatgcggc caagaatcag gttgcaatga accccaccaa cacagttttt 360gatgccaaac gtctgatcgg acgtaggttc gatgatgctg ttgttcagtc tgacatgaag 420cactggccct tcatggtggt gaacgatgca ggcaggccca aggtccaagt cgaatacaaa 480ggggagacaa aaagtttcta tcctgaggaa gtgtcttcaa tggttctgac aaaaatgaag 540gaaattgcag aagcttacct tggaaagact gttaccaatg ccgtggtcac cgtgccagct 600tacttcaatg actctcagcg acaggcagca aaagatgctg gaactattgc tggcctcaac 660gtacttcgaa ttatcaatga gccaactgct gctgctattg cctatggctt agataagaag 720gtcagggctg aaaggaatgt gctcattttt gacttgggag gtggcacttt tgatgtgtca 780attctgacta ctgaggatgg aatttttgaa gtcaaatcaa cagctggaga cacccacttg 840ggcggagaag actttgacaa ccgaatggtc aaccatttca ttgctgagtt taagcgaaag 900cacaagaagg acatcagtga gaacaagaga gctgtcaggc gtctccgcac tgcctgtgag 960cgggccaagc gcaccctctc ctccagcacc caggccagta ttgagattga ttctctctat 1020gagggaattg acttctacac ctccattacc cgtgctcgat ttgaggagtt gaatgctgac 1080ctgttccgtg gcacactgga ccctgtagag aaggcccttc gagatgccaa actagacaag 1140tcacagatcc atgatattgt cctggtgggt ggttctacca gaatccccaa gatccagaaa 1200cttctgcaag acttcttcaa tggaaaagag ctgaataaga gcattaaccc cgatgaagct 1260gttgcctatg gtgcagctgt ccaggcagcc attctatctg gagacaagtc tgagaatgtt 1320caggatttgc tgctcttgga tgtcactcct ctttcccttg ggattgaaac tgctggtgga 1380gtcatgactg tcctcatcaa gcgcaatacc accattccca ccaagcagac ccagactttc 1440accacctact ctgacaacca gccaggtgta ctcatccagg tgtatgaagg tgaaagggcc 1500atgaccaagg acaacaacct gcttgggaag tttgagctca caggcatacc tccagcaccc 1560cgtggggttc ctcagattga ggttactttt gacattgatg ccaatggcat cctcaatgtt 1620tctgctgtag ataagagcac aggaaaggag aacaagatca ccatcaccaa tgacaagggc 1680cgcttgagta aggaggatat tgagcgcatg gtccaagaag ctgagaagta caaagctgag 1740gatgagaagc agagagataa ggtttcctct aagaactcgc tggagtctta tgctttcaac 1800atgaaagcaa ctgttgagga tgagaaactt caaggcaaga tcaatgatga agacaaacag 1860aagattcttg acaagtgcaa cgaaatcatc agctggctgg ataagaacca gactgcggag 1920aaggaagaat ttgagcatca gcagaaagaa ctggagaagg tctgcaaccc tatcatcacc 1980aagctgtacc agagtgctgg tggcatgcct ggaggaatgc ctggtggctt ccctggtgga 2040ggagctcctc catctggtgg tgcttcttca ggccccacca ttgaagaggt cgattaagtc 2100aaagtagagg gtgtagcatt gttccacagg gacccaaaac aagtaacatg gaataataaa 2160actatttaaa ttggcacaaa aaaaaaaaaa aaaacggaga tgtggtgact cagctgtaat 2220cccggcactt aggaagctga gg 2242 108 374 PRT Rattus norvegicus Taumicrotubule associated protein 108 Met Ala Glu Pro Arg Gln Glu Phe AspThr Met Glu Asp Gln Ala Gly 1 5 10 15 Asp Tyr Thr Met Leu Gln Asp GlnGlu Gly Asp Met Asp His Gly Leu 20 25 30 Lys Ala Glu Glu Ala Gly Ile GlyAsp Thr Pro Asn Met Glu Asp Gln 35 40 45 Ala Ala Gly His Val Thr Gln AlaArg Val Ala Gly Val Ser Lys Asp 50 55 60 Arg Thr Gly Asn Asp Glu Lys LysAla Lys Gly Ala Asp Gly Lys Thr 65 70 75 80 Gly Ala Lys Ile Ala Thr ProArg Gly Ala Ala Thr Pro Gly Gln Lys 85 90 95 Gly Thr Ser Asn Ala Thr ArgIle Pro Ala Lys Thr Thr Pro Ser Pro 100 105 110 Lys Thr Pro Pro Gly SerGly Glu Pro Pro Lys Ser Gly Glu Arg Ser 115 120 125 Gly Tyr Ser Ser ProGly Ser Pro Gly Thr Pro Gly Ser Arg Ser Arg 130 135 140 Thr Pro Ser LeuPro Thr Pro Pro Thr Arg Glu Pro Lys Lys Val Ala 145 150 155 160 Val ValArg Thr Pro Pro Lys Ser Pro Ser Ala Ser Lys Ser Arg Leu 165 170 175 GlnThr Ala Pro Val Pro Met Pro Asp Leu Lys Asn Val Arg Ser Lys 180 185 190Ile Gly Ser Thr Glu Asn Leu Lys His Gln Pro Gly Gly Gly Lys Val 195 200205 Gln Ile Ile Asn Lys Lys Leu Asp Leu Ser Asn Val Gln Ser Lys Cys 210215 220 Gly Ser Lys Asp Asn Ile Lys His Val Pro Gly Gly Gly Ser Val Gln225 230 235 240 Ile Val Tyr Lys Pro Val Asp Leu Ser Lys Val Thr Ser LysCys Gly 245 250 255 Ser Leu Gly Asn Ile His His Lys Pro Gly Gly Gly GlnVal Glu Val 260 265 270 Lys Ser Glu Lys Leu Asp Phe Lys Asp Arg Val GlnSer Lys Ile Gly 275 280 285 Ser Leu Asp Asn Ile Thr His Val Pro Gly GlyGly Asn Lys Lys Ile 290 295 300 Glu Thr His Lys Leu Thr Phe Arg Glu AsnAla Lys Ala Lys Thr Asp 305 310 315 320 His Gly Ala Glu Ile Val His LysSer Pro Val Val Ser Gly Asp Thr 325 330 335 Ser Pro Arg His Leu Ser AsnVal Ser Ser Thr Gly Ser Ile Asp Met 340 345 350 Val Asp Ser Pro Gln LeuAla Thr Leu Ala Asp Glu Val Ser Ala Ser 355 360 365 Leu Ala Lys Gln GlyLeu 370 109 5240 DNA Rattus norvegicus Tau microtubule associatedprotein 109 cctcccctgg ggaggttcgc gttcccgtgg ctcgcgcctg ctgcccgccggcctcgagaa 60 cgcgctctct cggccgcggc gctcacagtc tccgccaccc accagctccagcaccagcag 120 cagcgccggc gccaccgccc accttctgct gtcgccgccg ccacaaccaccttcccctcc 180 gctgtcctct tctgtcctcg cctcctgtcg attatcaggc tttgaagcagcatggctgaa 240 ccccgccagg agtttgacac aatggaagac caggccggag attacactatgctccaagac 300 caagaaggag acatggacca tggcttaaaa gctgaagaag caggcatcggagacaccccg 360 aacatggagg accaagctgc tgggcatgtg actcaagctc gagtggccggcgtaagcaaa 420 gacaggacag gaaatgacga gaagaaagcg aagggggccg atggcaaaacgggggcgaag 480 atcgccacac ctcggggagc agccactccg ggccagaaag gcacatccaatgccaccagg 540 atcccagcca agaccacacc cagcccaaag actcctccag gatcaggtgaaccaccaaaa 600 tccggagaac gaagcggcta cagcagcccc ggctcgcccg gaacccctggcagtcgctcc 660 cgtaccccat ccctaccaac gccgcccacc cgagagccca aaaaggtggcagtggttcgc 720 actcccccta agtcaccgtc tgccagtaag agccgcctac agactgcccctgtgcccatg 780 ccagacctaa agaacgtcag gtccaagatt ggctccactg agaacctgaagcaccagccg 840 ggaggcggca aggtgcagat aattaataag aagctggatc ttagcaacgtccagtccaag 900 tgtggctcaa aggacaatat caaacacgtc ccgggcggag gcagtgtgcaaatagtctac 960 aagccagtgg acctgagcaa ggtgacctcc aagtgtggtt ccttagggaacatccatcac 1020 aagccaggag gtggccaggt agaagtaaaa tcagagaagc tggacttcaaggatagagtc 1080 cagtcgaaga ttggctcctt ggataacatc acccatgtcc ctggaggagggaataagaag 1140 attgaaaccc acaagctgac cttcagggag aatgccaaag ccaagacagaccatggagca 1200 gaaatcgtgc acaagtcacc tgtggtgtct ggggacacat ctccacggcacctcagcaac 1260 gtctcctcca cgggcagcat cgacatggtg gactctccac agcttgccacgttagccgat 1320 gaagtgtccg cctctttggc caagcagggt ttgtgatcag gcccctggggccgtcactga 1380 tcatggagag aagagagagt gagagtgtgg aaaaaaaaaa aaaaagaatgacctggcccc 1440 tcaccctctg ccctccccgc tgctcctcat agacaggctg accagcttgtcacctaacct 1500 gcttttgtgg ctcgggtttg gctcgggact tcaaaatcag tgatgggaaaaagtaaattt 1560 catctttcca aattgatttg tgggctagta ataaaatatt tttaaggaaggaaaaaaaaa 1620 acacgtaaaa ccatggccaa acaaaaccca acatttcctt ggcaattgttattgaccccg 1680 cccccccctc tgagttttag agggtgaagg aggctttgga tagaggctgcttctggggat 1740 tggctgaggg actagggcaa ctaattgccc acagccccat cttaggggcatcagggacag 1800 cggcagacat gaaagacttg ggacttggtg tgtttgtgga gccgtaaggcgtatgttaac 1860 tttgtgtggg tttgagggag gactgtgata gtgaaggctg agagatgggtgggctgggag 1920 tcagaggaga gaggtgagga agacaggttg ggagaggggg cattgcgtccttgccaagga 1980 gcttgggaag cacaggtagc cctggctgca gcagtcttag ctagcacagatgcctgcctg 2040 agaaagcaca gtggggtaca gtgggtgtgt gtgccccttc tgaagggcagcccatgggag 2100 aaggggtatt gggcagaagg aaggtaggcc ccagaaggtg gcaccttgtagattggttct 2160 ctgaaggctg accttgccat cccagggcac tgctcccacc ctccaggaggaggtctgagc 2220 tgaggagctt ccttttcgat ctcacaggaa aacctgtgtt actgagttctgaagtttgga 2280 actacagcca tgattttggc caccatacag acctgggact ttagggctaaccagttcttt 2340 gtaaggactt gtgcctcttg cgggaacatc tgcctgttct caagcctggtcctctggcac 2400 ttctgcagtg gtgagggatg ggggtggtat tctgggatgt gggtcccaggcctcccatcc 2460 ctcgcacagc cactgtatcc cctctacctg tcctatcatg cccacgtctgccacgagagc 2520 cagtcactgc cgtccgtaca tcacgtctca ccgtcctgag tgcccagcctcccaagccca 2580 atccctggac ccctgggtag ttatggccaa tctgctctac actaggggttggagtccagg 2640 gaaggcaaag atttgggcct tggtctctag tcctacgttg ccagaatccaaccagtgtgc 2700 ctcccacaag gaaccttaca accttgtttg gtttgctcca tcaggcgtttggcgccatcg 2760 tggatggagt ccgtgtgtgc ctggagatta ccctggacac ctctgcttttttttttttta 2820 ctttagcggt tgcctcctag gcctgactcc ttcccatgtt gaactggaggcagccaagtt 2880 aggtgtcaat gtcctggcat cagtatgaac agtcagtagt cccagggcagggccacactt 2940 ctcccatctt ctgcttccac cccagcttgt gattgctagc ctcccagagctcagccgcca 3000 ttaagtcccc atgcacgtaa tcagcccttc cataccccaa tttggggaacataccccttg 3060 attgaaatgt tttccctcca gtcctatgga agcggtgctg cctgcctgctggagcagcca 3120 gccatctcag agacgcagcc ctttctctcc tgtccgcacc ctgctgcgctgtagtcggat 3180 tcgtctgttt gtctgggttc accagagtga ctatgatagt gaaaagaaaaagaaaaagaa 3240 aaaagaaaaa agaaaaaaaa aaaaggacgc atgttatctt gaaatatttgtcaaaaggtt 3300 gtagcccacc gcagggattg gagggcctga tattccttgt cttcttcgtgacttaggtcc 3360 aggccggtcg agtgctaccc tgctggacat cccatgtttt gaagggtttcttcttcatct 3420 gggacccctg cagacactgg attgtgacat tggaggtcta tacattggccaaggctgaag 3480 cacaggaccc gttagaggca gcaggctccg actgtcaggg agagcttgtggctggcctgt 3540 ttctctgagt gaagatggtc ctctctaatc acaacttcaa gtcccacagcagccctggca 3600 gacatctaag aactcctgca tcacaagaga aaaggacact agtaccagcagggagagctg 3660 tggccctaga aattccatga ctctccacta ctatccgtgg gtcctttccaagccttgcct 3720 cgtcaccaag ggcttgggat ggactgcccc actgatgaaa gggacatctttggagacccc 3780 cttggtttcc aaggcgtcag ccccctgacc ttgcatgacc tcctacagctgaaggatgag 3840 gcctttaaag attaggaacc tcaggcccag gtcggccact ttgggcttgggtacagttag 3900 ggacgatgcg gtagaaggag gtggccaacc tttccatata agagttctgtgtgcccagag 3960 ctaccctatt gtgagctccc cactgctgat ggactttagc tgtccttagaagtgaagagt 4020 ccaacggagg aaaaggaagt gtggtttgat ggtctgtggt cccttcatcatggttacctg 4080 ttgtggtttt ctctgtatac ccccatttac ccatcctgca gttcctgtccttgaataggg 4140 gtgggggtac tctgccatat ctcttgtagg cagtcagccc ccaagtcatagtttggagtg 4200 atctggtcag tgctaatagg cagtttacaa ggaattctgg cttgttacttcagtgaggac 4260 aatcccccaa ggccctggca cctgtcctgt ctttccatgg ctctccactgcagagccaat 4320 gtctttgggt gggctagata gggtgtacaa tttgcctgga acctccaagctcttaatcca 4380 ctttatcaat agttccattt aaattgactt caatataaga gtgtatccatttgagattgc 4440 ttgtgttgtg gggtaaaggg gggaggagga acatgttaag ataattgacatgggcaaggg 4500 gaagtcttga agtgtagcag ttaaaccatc ttgtagcccc attcatgatgttgaccactt 4560 gctagagaga agaggtgcca taaggctaga acctagaggc ttggctgtccaccaacaggc 4620 aggcttttgc aaggcagagg cagccagcta ggtccctgac ttcccagccaggtgcagctc 4680 taagaactgc tcttgcctgc tgccttcttg tggtgtccag agcccacagccaatgcctcc 4740 tcaaaaccct ggcttccttc cttctaatcc actggcacat cagcatcacctccggattga 4800 cttcagatcc acagcctaca ctactagcag tgggtaagac cacttcctttgtccttgtct 4860 gttctccaga aaagtgggca tggaggcggt gttaataact ataggtctgtggctttatga 4920 gccttcaaac ttctctctag cttctgaaag ggttactttt gggcagtattgcagtctcac 4980 cctccgatgg ctgtagcctg tgcagttgct gtactgggca tgatctccagtgcttgcaag 5040 tcccatgatt tctttggtgt tttgagggtg gggggaggga catgaatcatcttagcttag 5100 cttcctgtct gtgaatgtcc atatagtgta ctgtgtttta acaaacgatttacactgact 5160 gttgctgtac aagtgaattt ggaaataaag ttattactct gattaaacaaaaaaaaaaaa 5220 aaaaaaaaaa aaaaaaaaaa 5240 110 248 PRT Rattus norvegicusSchwann cell peripheral myelin 110 Met Ala Pro Gly Ala Pro Ser Ser SerPro Ser Pro Ile Leu Ala Ala 1 5 10 15 Leu Leu Phe Ser Ser Leu Val LeuSer Pro Thr Leu Ala Ile Val Val 20 25 30 Tyr Thr Asp Arg Glu Val Tyr GlyAla Val Arg Ser Gln Val Thr Leu 35 40 45 His Cys Ser Phe Trp Ser Ser GluTrp Val Ser Asp Asp Ile Ser Phe 50 55 60 Thr Trp Arg Tyr Gln Pro Glu GlyGly Arg Asp Ala Ile Ser Ile Phe 65 70 75 80 His Tyr Ala Lys Gly Gln ProTyr Ile Asp Glu Val Gly Thr Phe Lys 85 90 95 Glu Arg Ile Gln Trp Val GlyAsp Pro Ser Trp Lys Asp Gly Ser Ile 100 105 110 Val Ile His Asn Leu AspTyr Ser Asp Asn Gly Thr Phe Thr Cys Asp 115 120 125 Val Lys Asn Pro ProAsp Ile Val Gly Lys Thr Ser Gln Val Thr Leu 130 135 140 Tyr Val Phe GluLys Val Pro Thr Arg Tyr Gly Val Val Leu Gly Ala 145 150 155 160 Val IleGly Gly Ile Leu Gly Val Val Leu Leu Leu Leu Leu Leu Phe 165 170 175 TyrLeu Ile Arg Tyr Cys Trp Leu Arg Arg Gln Ala Ala Leu Gln Arg 180 185 190Arg Leu Ser Ala Met Glu Lys Gly Lys Phe His Lys Ser Ser Lys Asp 195 200205 Ser Ser Lys Arg Gly Arg Gln Thr Pro Val Leu Tyr Ala Met Leu Asp 210215 220 His Ser Arg Ser Thr Lys Ala Ala Ser Glu Lys Lys Ser Lys Gly Leu225 230 235 240 Gly Glu Ser Arg Lys Asp Lys Lys 245 111 1029 DNA Rattusnorvegicus Schwann cell peripheral myelin 111 attcggctgg gcccttgcccctaccccagc tatggctcct ggggctccct catccagccc 60 cagccctatc ctggctgccctgctcttctc ttctttggtg ctgtccccaa ccctggccat 120 tgtggtttac acggacagggaagtctatgg tgctgtgcgc tcccaggtca ccctgcactg 180 ctccttctgg tccagtgaatgggtctcaga tgacatctct tttacctggc gctaccagcc 240 tgaaggaggc cgagatgccatttcaatctt ccactatgcc aagggtcaac cttacatcga 300 tgaggtgggg accttcaaggagcgcatcca gtgggtaggg gaccctagct ggaaggatgg 360 ctccattgtc atacacaacctagactacag tgacaacggc actttcacat gtgatgtcaa 420 aaacccaccg gacatagtgggcaagacgtc tcaggtcacg ctctatgtct ttgaaaaagt 480 gcccactagg tatggggtggtgttgggagc cgtgatcggt ggcatcctcg gggtggtgct 540 gttgctgctg ttgctcttctacctgatccg gtactgctgg ctgcgcaggc aggctgccct 600 gcagaggagg ctcagtgccatggagaaggg gaaatttcac aagtcttcta aggactcctc 660 gaagcgcggg cggcagacgccagtgctgta tgccatgctg gaccacagcc gaagcaccaa 720 agctgccagt gagaagaaatctaaagggct gggggagtct cgcaaggata agaaatagcg 780 gttagcgggc cgggcggggggtcgggggtc tgcgatggag tcttccaaag gctctcaggt 840 ggtggtcatc gagatggagcttcgcaaaga tgagcagagc tcggagcccc ggcctgcagt 900 caaatccccc agtagaaccagcctcaagaa cgccctcaag aacatgatgg gcctggactc 960 gaacaagtga ccgtcacctccaggctctgt cagagcaggg gacctaggct cctctttttc 1020 ccagtctag 1029 112 444PRT Rattus norvegicus Class 1 beta tubulin 112 Met Arg Glu Ile Val HisIle Gln Ala Gly Gln Cys Gly Asn Gln Ile 1 5 10 15 Gly Ala Lys Phe TrpGlu Val Ile Ser Asp Glu His Gly Ile Asp Pro 20 25 30 Thr Gly Thr Tyr HisGly Asp Ser Asp Leu Gln Leu Asp Arg Ile Ser 35 40 45 Val Tyr Tyr Asn GluAla Thr Gly Gly Lys Tyr Val Pro Arg Ala Ile 50 55 60 Leu Val Asp Leu GluPro Gly Thr Met Asp Ser Val Arg Ser Gly Pro 65 70 75 80 Phe Gly Gln IlePhe Arg Pro Asp Asn Phe Val Phe Gly Gln Ser Gly 85 90 95 Ala Gly Asn AsnTrp Ala Lys Gly His Tyr Thr Glu Gly Ala Glu Leu 100 105 110 Val Asp SerVal Leu Asp Val Val Arg Lys Glu Ala Glu Ser Cys Asp 115 120 125 Cys LeuGln Gly Phe Gln Leu Thr His Ser Leu Gly Gly Gly Thr Gly 130 135 140 SerGly Met Gly Thr Leu Leu Ile Ser Lys Ile Arg Glu Glu Tyr Pro 145 150 155160 Asp Arg Ile Met Asn Thr Phe Ser Val Val Pro Ser Pro Lys Val Ser 165170 175 Asp Thr Val Val Glu Pro Tyr Asn Ala Thr Leu Ser Val His Gln Leu180 185 190 Val Glu Asn Thr Asp Glu Thr Tyr Cys Ile Asp Asn Glu Ala LeuTyr 195 200 205 Asp Ile Cys Phe Arg Thr Leu Lys Leu Thr Thr Pro Thr TyrGly Asp 210 215 220 Leu Asn His Leu Val Ser Ala Thr Met Ser Gly Val ThrThr Cys Leu 225 230 235 240 Arg Phe Pro Gly Gln Leu Asn Ala Asp Leu ArgLys Leu Ala Val Asn 245 250 255 Met Val Pro Phe Pro Arg Leu His Phe PheMet Pro Gly Phe Ala Pro 260 265 270 Leu Thr Ser Arg Gly Ser Gln Gln TyrArg Ala Leu Thr Val Pro Glu 275 280 285 Leu Thr Gln Gln Val Phe Asp AlaLys Asn Met Met Ala Ala Cys Asp 290 295 300 Pro Arg His Gly Arg Tyr LeuThr Val Ala Ala Val Phe Arg Gly Arg 305 310 315 320 Met Ser Met Lys GluVal Asp Glu Gln Met Leu Asn Val Gln Asn Lys 325 330 335 Asn Ser Ser TyrPhe Val Glu Trp Ile Pro Asn Asn Val Lys Thr Ala 340 345 350 Val Cys AspIle Pro Pro Arg Gly Leu Lys Met Ala Val Thr Phe Ile 355 360 365 Gly AsnSer Thr Ala Ile Gln Glu Leu Phe Lys Arg Ile Ser Glu Gln 370 375 380 PheThr Ala Met Phe Arg Arg Lys Ala Phe Leu His Trp Tyr Thr Gly 385 390 395400 Glu Gly Met Asp Glu Met Glu Phe Thr Glu Ala Glu Ser Asn Met Asn 405410 415 Asp Leu Val Ser Glu Tyr Gln Gln Tyr Gln Asp Ala Thr Ala Glu Glu420 425 430 Glu Glu Asp Phe Gly Glu Glu Ala Glu Glu Glu Ala 435 440 1132346 DNA Rattus norvegicus Class 1 beta tubulin 113 ctcgcatctctctgcagcag cctccggggc tacgttccat cctatcagtc tgagaccagc 60 ccagaagaaaaaaaccaaaa atccttaatt atcttctttt gctcggtacc tacattggga 120 ccatcaaaaaaaaattatta cagtaaaccg tagccatgag ggaaatcgtg cacatccagg 180 ccggacagtgtggcaaccag atcggtgcta agttctggga ggtgataagc gatgaacacg 240 gcatcgaccccaccggcacc taccacggag acagcgactt gcagctggac cgaatctctg 300 tgtactacaatgaagctaca ggtggcaagt atgtccctcg agctatctta gtggatctag 360 aacccgggactatggactcc gttcgctcag gtccttttgg ccagatcttc agaccggaca 420 actttgtttttggtcagtct ggggcaggca acaactgggc taagggtcac tacacagagg 480 gagctgagctggttgactct gtcttggatg tggtgcggaa ggaggcggag agctgtgact 540 gcctgcaaggctttcagctg acccactcgc tgggtggagg cacgggctct ggcatgggca 600 ccctgctcatcagcaagatt cgagaagaat accccgaccg catcatgaac accttcagcg 660 tggtgccctcacccaaagtg tctgacaccg tggttgagcc ctacaacgcc accctgtccg 720 tccaccagttggtagagaac acagatgaga cctactgcat tgacaacgag gctctctacg 780 acatctgcttccgtaccctt aagctcacca cgccaaccta cggagacctg aaccatctcg 840 tctctgccaccatgagcggt gtcaccacct gcctccgttt cccgggccag ctgaacgccg 900 accttcggaagctggctgtc aacatggtgc ccttcccgcg tctccacttc ttcatgcctg 960 gctttgcccctctcaccagc cgtggaagcc agcagtaccg ggccctcact gtgcctgaac 1020 tcacccagcaggtcttcgac gccaagaaca tgatggccgc ctgtgaccca cgccacggcc 1080 ggtacctcacggttgcggct gtcttccgtg gacggatgtc catgaaggag gtagatgagc 1140 aaatgctcaacgtgcagaat aagaatagca gctacttcgt ggaatggatc cccaacaacg 1200 tcaagacggctgtctgtgac atcccaccgc gaggcctcaa gatggcagtc accttcatcg 1260 gaaacagcacggccatccag gagctcttca agcgcatctc tgagcagttt acggcaatgt 1320 tccgtcggaaggctttcctc cactggtaca cgggtgaggg catggacgag atggagttca 1380 ccgaagctgagagcaacatg aatgacctcg tctctgagta tcagcagtac caggacgcca 1440 ccgcagaagaggaagaggat ttcggagagg aggcagaaga agaggcctaa ggcggaagcc 1500 ctgcgtcacctcaggctgct tagttccctt agcttttttc caactgccct ttgtttcttt 1560 ctgctgcctctgtcctgttt ttgttccgtt ttgttttctc attggggggt aaacggtgcc 1620 tggcacgtggcaggcgctca ataaatattt ctttgtggaa tgtctccttt ctctttccga 1680 tctgacattctgagcgttaa ccctgtgctt cctcctggta cccatttctt tattatgact 1740 gtccagttaatattcttcca atatattctc caggaaccag cgttcggctc cagacccggg 1800 gtagaacccagcccagttct aagaactcaa gagagtagcc accatcttaa agcaaagtag 1860 cgggtggggaaaaggtgggt agaggagatg ctacttaggg ggtgggattt tccagtcagg 1920 gccatttagacacgggaatc gctggggctt tcagtcgatg gggctttctt ttttccctgc 1980 ttcatctctcggcctcagga gaggtattaa cagtattatc accatttata tcctagctgt 2040 cctgagccaaatctgccatt ggaggtgtct tcccctgtgt tggttctcca agggacgttg 2100 tatggggatttgtgggggca gggcagcaag gccttgttct ctaaatgtcc agaaacactc 2160 taattaccatttccacctgg gtgcctcaca cactccccag aggggaggta acatctctgc 2220 cccatttccatcctgttcct tggcttggtc attccctacc tttctatttt ctgttaaact 2280 tggctttttttttttcatat tgaaaagatg acattgcccc gagagccaaa aataaatggg 2340 aattgg 2346114 724 PRT Rattus norvegicus Heat shock protein 90 114 Met Pro Glu GluVal His His Gly Glu Glu Glu Val Glu Thr Phe Ala 1 5 10 15 Phe Gln AlaGlu Ile Ala Gln Leu Met Ser Leu Ile Ile Asn Thr Phe 20 25 30 Tyr Ser AsnLys Glu Ile Phe Leu Arg Glu Leu Ile Ser Asn Ala Ser 35 40 45 Asp Ala LeuAsp Lys Ile Arg Tyr Glu Ser Leu Thr Asp Pro Ser Lys 50 55 60 Leu Asp SerGly Lys Glu Leu Lys Ile Asp Ile Ile Pro Asn Pro Gln 65 70 75 80 Glu AlaThr Leu Thr Leu Val Asp Thr Gly Ile Gly Met Thr Lys Ala 85 90 95 Asp LeuIle Asn Asn Leu Gly Thr Ile Ala Lys Ser Gly Thr Lys Ala 100 105 110 PheMet Glu Ala Leu Gln Ala Gly Ala Asp Ile Ser Met Ile Gly Gln 115 120 125Phe Gly Val Gly Phe Tyr Ser Ala Tyr Leu Val Ala Glu Lys Val Val 130 135140 Val Ile Thr Lys His Asn Asp Asp Glu Gln Tyr Ala Trp Glu Ser Ser 145150 155 160 Ala Gly Gly Ser Phe Thr Val Arg Ala Asp His Gly Glu Pro IleGly 165 170 175 Arg Gly Thr Lys Val Ile Leu His Leu Lys Glu Asp Gln ThrGlu Tyr 180 185 190 Leu Glu Glu Arg Arg Val Lys Glu Val Val Lys Lys HisSer Gln Phe 195 200 205 Ile Gly Tyr Pro Ile Thr Leu Tyr Leu Glu Lys GluArg Glu Lys Glu 210 215 220 Ile Ser Asp Asp Glu Ala Glu Glu Glu Lys GlyGlu Lys Glu Glu Glu 225 230 235 240 Asp Lys Glu Asp Glu Glu Lys Pro LysIle Glu Asp Val Gly Ser Asp 245 250 255 Glu Glu Asp Asp Ser Gly Lys AspLys Lys Lys Lys Thr Lys Lys Ile 260 265 270 Lys Glu Lys Tyr Ile Asp GlnGlu Glu Leu Asn Lys Thr Lys Pro Ile 275 280 285 Trp Thr Arg Asn Pro AspAsp Ile Thr Gln Glu Glu Tyr Gly Glu Phe 290 295 300 Tyr Lys Ser Leu ThrAsn Asp Trp Glu Asp His Leu Ala Val Lys His 305 310 315 320 Phe Ser ValGlu Gly Gln Leu Glu Phe Arg Ala Leu Leu Phe Ile Pro 325 330 335 Arg ArgAla Pro Phe Asp Leu Phe Glu Asn Lys Lys Lys Lys Asn Asn 340 345 350 IleLys Leu Tyr Val Arg Arg Val Phe Ile Met Asp Ser Cys Asp Asp 355 360 365Leu Ile Pro Glu Tyr Leu Asn Phe Ile Arg Gly Val Val Asp Ser Glu 370 375380 Asp Leu Pro Leu Asn Ile Ser Arg Glu Met Leu Gln Gln Ser Lys Ile 385390 395 400 Leu Lys Val Ile Arg Lys Asn Ile Val Lys Lys Cys Leu Glu LeuPhe 405 410 415 Ser Glu Leu Ala Glu Asp Lys Glu Asn Tyr Lys Lys Phe TyrGlu Ala 420 425 430 Phe Ser Lys Asn Leu Lys Leu Gly Ile His Glu Asp SerThr Asn Arg 435 440 445 Arg Arg Leu Ser Glu Leu Leu Arg Tyr His Thr SerGln Ser Gly Asp 450 455 460 Glu Met Thr Ser Leu Ser Glu Tyr Val Ser ArgMet Lys Glu Thr Gln 465 470 475 480 Lys Ser Ile Tyr Tyr Ile Thr Gly GluSer Lys Glu Gln Val Ala Asn 485 490 495 Ser Ala Phe Val Glu Arg Val ArgLys Arg Gly Phe Glu Val Val Tyr 500 505 510 Met Thr Glu Pro Ile Asp GluTyr Cys Val Gln Gln Leu Lys Glu Phe 515 520 525 Asp Gly Lys Ser Leu ValSer Val Thr Lys Glu Gly Leu Glu Leu Pro 530 535 540 Glu Asp Glu Glu GluLys Lys Lys Met Glu Glu Ser Lys Ala Arg Phe 545 550 555 560 Glu Asn LeuCys Lys Leu Met Lys Glu Ile Leu Asp Lys Lys Val Glu 565 570 575 Lys ValThr Ile Ser Asn Arg Leu Val Ser Ser Pro Cys Cys Ile Val 580 585 590 ThrSer Thr Tyr Gly Trp Thr Ala Asn Met Glu Arg Ile Met Lys Ala 595 600 605Gln Ala Leu Arg Asp Asn Ser Thr Met Gly Tyr Met Met Ala Lys Lys 610 615620 His Leu Glu Ile Asn Pro Asp His Pro Ile Val Glu Thr Leu Arg Gln 625630 635 640 Lys Ala Glu Ala Asp Lys Asn Asp Lys Ala Val Lys Asp Leu ValVal 645 650 655 Leu Leu Phe Glu Thr Ala Leu Ser Ser Leu Ala Ser His PheArg Arg 660 665 670 Pro Lys Thr His Ser Asn Arg Ile Tyr Arg Met Ile LysLeu Gly Leu 675 680 685 Gly Ile Asp Glu Asp Glu Val Thr Ala Glu Glu ProSer Ala Ala Val 690 695 700 Pro Asp Glu Ile Pro Pro Leu Glu Gly Asp GluAsp Ala Ser Arg Met 705 710 715 720 Glu Glu Val Asp 115 2524 DNA Rattusnorvegicus Heat shock protein 90 115 ggcctaggct tgccgtgcga gtcggacgtggtccgggccc accctgctct gtactactac 60 tcggctttct cgtcaagatg cctgaggaagtgcaccatgg agaggaagag gtggagacct 120 tcgcctttca ggcagaaatt gcccagctgatgtccctcat catcaacact ttttactcaa 180 acaaagagat tttcctccgg gagttgatctctaatgcttc tgatgccctg gacaagattc 240 ggtatgagag cctgactgac ccttccaagttggacagcgg gaaagagctg aagattgaca 300 tcatccccaa ccctcaggaa gccacgctgactttggtgga cacaggcatt ggcatgacca 360 aggctgacct cataaataac ttgggaaccattgctaagtc tggtacaaag gcgttcatgg 420 aggctctcca ggctggtgca gacatctccatgattgggca gtttggtgtc ggcttctact 480 ctgcctacct ggtggcagag aaagtggttgtgatcacgaa gcacaacgat gatgagcagt 540 atgcctggga gtcttctgct ggtggatccttcactgtccg tgcagaccac ggtgagccca 600 ttggccgggg taccaaagtg atccttcacctcaaagaaga ccagacagag tacttagagg 660 agaggagggt caaggaggtg gtgaagaaacactcacagtt cataggctac cccatcaccc 720 tctatctgga gaaggaacgc gagaaggagatcagtgatga tgaggcagag gaagagaaag 780 gggagaagga ggaggaagat aaggaggatgaggagaagcc taagattgag gatgtgggat 840 ctgatgagga ggatgacagc ggcaaagataagaaaaagaa aacaaagaag atcaaggaga 900 agtacattga ccaggaagag ctgaataagacgaagcccat ctggaccaga aatcctgatg 960 acatcactca agaggaatat ggtgaattctacaagagcct caccaatgac tgggaagacc 1020 acttggcagt caagcacttc tctgtagaagggcagttgga attcagggca ttgctcttca 1080 ttccccggcg agctcccttt gacctctttgagaacaagaa gaagaagaac aacatcaaat 1140 tgtatgtccg tcgtgtgttc atcatggacagctgtgatga cctgatacct gagtacctca 1200 acttcatccg tggtgtggtt gattccgaggacctgcctct gaacatctcc cgagaaatgc 1260 tccagcagag caagatcctg aaagtcatccgcaaaaacat tgtgaagaag tgccttgagc 1320 tcttctctga gttggctgaa gacaaagagaactacaagaa attttatgag gcattctcta 1380 agaatttaaa gcttggaatc catgaggattccactaaccg tcgccgcctc tctgagctcc 1440 ttcgctacca tacctctcag tctggagatgagatgacttc cctgtcagag tatgtgtctc 1500 gcatgaagga gacacagaag tccatctactatatcactgg tgagagcaaa gagcaggtgg 1560 ccaactctgc cttcgtggag cgtgtgcggaaacggggctt tgaggtggta tacatgactg 1620 agcccattga tgagtactgc gtacagcagctcaaggagtt cgatggcaag agcctggtct 1680 ccgtgaccaa ggagggcctg gagctaccagaggatgagga agagaagaaa aaaatggagg 1740 agagcaaggc aaggtttgag aatctctgcaagctcatgaa ggagatcttg gacaagaagg 1800 ttgaaaaagt gactatctcc aataggcttgtgtcttcccc ctgctgcatt gtgaccagca 1860 cctacggctg gacagccaac atggaacggattatgaaggc ccaggcactg cgggacaact 1920 cgacaatggg ctacatgatg gccaagaaacacctagagat caaccctgac caccccattg 1980 tggagactct gcggcagaag gctgaggcagacaaaaacga caaagctgtc aaagacctgg 2040 tggtgctgct gtttgagact gctctgtcttctctcgcttc tcactttagg aggcccaaaa 2100 cccactccaa ccgcatctac cgcatgattaaactaggcct gggcattgat gaagatgagg 2160 tcactgcaga agagcccagt gctgctgttcccgatgagat ccccccactg gagggtgatg 2220 aggatgcctc tcgcatggaa gaagtggattaaaggctcct gggaagcccc cgccctctgt 2280 atagtatccc tgtggctccc ccaacagccttgactggccc tgacccacct gggctctctc 2340 tgctcatgtc tacaagaatc tcttctatcctgtccttgtg ccttaaggca ggaagatccc 2400 ctcccacaga tagcagggtt gggtgttgtgtattgtgttt ttttgtttgt tttattttgt 2460 tctgaaatta aaagtatgca aaataaagatgatgcagttt taaaaaaaaa aaaaaaaaaa 2520 aagg 2524 116 541 PRT Homo sapiensPutative ribonuclease III 116 Met Arg Arg Glu Val Thr Val Glu Leu SerSer Gln Gly Phe Trp Lys 1 5 10 15 Thr Gly Ile Arg Ser Asp Val Cys GlnHis Ala Met Met Leu Pro Val 20 25 30 Leu Thr His His Ile Arg Tyr His GlnCys Leu Met His Leu Asp Lys 35 40 45 Leu Ile Gly Tyr Thr Phe Gln Asp ArgCys Leu Leu Gln Leu Ala Met 50 55 60 Thr His Pro Ser His His Leu Asn PheGly Met Asn Pro Asp His Ala 65 70 75 80 Arg Asn Ser Leu Ser Asn Cys GlyIle Arg Gln Pro Lys Tyr Gly Asp 85 90 95 Arg Lys Val His His Met His MetArg Lys Lys Gly Ile Asn Thr Leu 100 105 110 Ile Asn Ile Met Ser Arg LeuGly Gln Asp Asp Pro Thr Pro Ser Arg 115 120 125 Ile Asn His Asn Glu ArgLeu Glu Phe Leu Gly Asp Ala Val Val Glu 130 135 140 Phe Leu Thr Ser ValHis Leu Tyr Tyr Leu Phe Pro Ser Leu Glu Glu 145 150 155 160 Gly Gly LeuAla Thr Tyr Arg Thr Ala Ile Val Gln Asn Gln His Leu 165 170 175 Ala MetLeu Ala Lys Lys Leu Glu Leu Asp Arg Phe Met Leu Tyr Ala 180 185 190 HisGly Pro Asp Leu Cys Arg Glu Ser Asp Leu Arg His Ala Met Ala 195 200 205Asn Cys Phe Glu Ala Leu Ile Gly Ala Val Tyr Leu Glu Gly Ser Leu 210 215220 Glu Glu Ala Lys Gln Leu Phe Gly Arg Leu Leu Phe Asn Asp Pro Asp 225230 235 240 Leu Arg Glu Val Trp Leu Asn Tyr Pro Leu His Pro Leu Gln LeuGln 245 250 255 Glu Pro Asn Thr Asp Arg Gln Leu Ile Glu Thr Ser Pro ValLeu Gln 260 265 270 Lys Leu Thr Glu Phe Glu Glu Ala Ile Gly Val Ile PheThr His Val 275 280 285 Arg Leu Leu Ala Arg Ala Phe Thr Leu Arg Thr ValGly Phe Asn His 290 295 300 Leu Thr Leu Gly His Asn Gln Arg Met Glu PheLeu Gly Asp Ser Ile 305 310 315 320 Met Gln Leu Val Ala Thr Glu Tyr LeuPhe Ile His Phe Pro Asp His 325 330 335 His Glu Gly His Leu Thr Leu LeuArg Ser Ser Leu Val Asn Asn Arg 340 345 350 Thr Gln Ala Lys Val Ala GluGlu Leu Gly Met Gln Glu Tyr Ala Ile 355 360 365 Thr Asn Asp Lys Thr LysArg Pro Val Ala Leu Arg Thr Lys Thr Leu 370 375 380 Ala Asp Leu Leu GluSer Phe Ile Ala Ala Leu Tyr Ile Asp Lys Asp 385 390 395 400 Leu Glu TyrVal His Thr Phe Met Asn Val Cys Phe Phe Pro Arg Leu 405 410 415 Lys GluPhe Ile Leu Asn Gln Asp Trp Asn Asp Pro Lys Ser Gln Leu 420 425 430 GlnGln Cys Cys Leu Thr Leu Arg Thr Glu Gly Lys Glu Pro Asp Ile 435 440 445Pro Leu Tyr Lys Thr Leu Gln Thr Val Gly Pro Ser His Ala Arg Thr 450 455460 Tyr Thr Val Ala Val Tyr Phe Lys Gly Glu Arg Ile Gly Cys Gly Lys 465470 475 480 Gly Pro Ser Ile Gln Gln Ala Glu Met Gly Ala Ala Met Asp AlaLeu 485 490 495 Glu Lys Tyr Asn Phe Pro Gln Met Ala His Gln Lys Arg PheIle Glu 500 505 510 Arg Lys Tyr Arg Gln Glu Leu Lys Glu Met Arg Trp GluArg Glu His 515 520 525 Gln Glu Arg Glu Pro Asp Glu Thr Glu Asp Ile LysLys 530 535 540 117 1626 DNA Homo sapiens Putative ribonuclease III 117atgagacgag aagtaacggt ggagctaagt agccaaggat tctggaaaac tggcatccgt 60tctgatgtct gtcagcatgc aatgatgcta cctgttctga cccatcatat ccgctaccac 120caatgcctaa tgcatttgga caagttgata ggatatactt tccaagatcg ttgtctgttg 180cagctggcca tgactcatcc aagtcatcat ttaaattttg gaatgaatcc tgatcatgcc 240aggaattcat tatctaactg tggaattcgg cagcccaaat acggagacag aaaagttcat 300cacatgcaca tgcggaagaa agggattaac accttgataa atatcatgtc acgccttggc 360caagatgacc caactccctc gaggattaac cacaatgaac ggttggaatt cctgggtgat 420gctgttgttg aatttctgac cagcgtccat ttgtactatt tgtttcctag tctggaagaa 480ggaggattag caacctatcg gactgccatt gttcagaatc agcaccttgc catgctagca 540aagaaacttg aactggatcg atttatgctg tatgctcacg ggcctgacct ttgtagagaa 600tcggaccttc gacatgcaat ggccaattgt tttgaagcgt taataggagc tgtttacttg 660gagggaagcc tggaggaagc caagcagtta tttggacgct tgctctttaa tgatccggac 720ctgcgcgaag tctggctcaa ttatcctctc cacccactcc aactacaaga gccaaatact 780gatcgacaac ttattgaaac ttctccggtt ctacaaaaac ttactgagtt tgaagaagca 840attggagtaa tttttactca tgttcgactt ctggcaaggg cattcacatt gagaactgtg 900ggatttaacc atctgaccct aggccacaat cagagaatgg aattcctagg tgactccata 960atgcaactgg tagccacaga gtacttattc attcatttcc cagatcatca tgaaggacac 1020ttaactttgt tgcgaagctc tttggtgaat aatagaactc aggccaaggt agcggaggag 1080ctgggcatgc aggagtacgc cataaccaac gacaagacca agaggcctgt ggcgcttcgc 1140accaagacct tggcggacct tttggaatca tttattgcag cgctgtacat tgataaggat 1200ttggaatatg ttcatacttt catgaatgtc tgcttctttc cacgattgaa agagttcatt 1260ttgaatcagg attggaatga ccccaaatcc cagcttcagc agtgttgctt gacacttagg 1320acagaaggaa aagagccaga cattcctctg tacaagactc tgcagacagt gggcccatcc 1380catgcccgaa cctacactgt ggctgtttat ttcaagggag aaagaatagg ctgtgggaaa 1440ggaccaagta ttcagcaagc ggaaatggga gcagcaatgg atgcgcttga aaaatataat 1500tttccccaga tggcccatca gaagcggttc atcgaacgga agtacagaca agagttaaaa 1560gaaatgaggt gggaaagaga gcatcaagag agagagccag atgagactga agacatcaag 1620aaataa 1626

1. Use of: (a) an isolated gene sequence that is down-regulated in the spinal cord of a mammal in response to mechanistically distinct first and second models of neuropathic or central sensitization pain; (b) an isolated gene sequence comprising a nucleic acid sequence of any of Tables I to VI; (c) an isolated gene sequence having at least 80% sequence identity with a nucleic acid sequence of any of Tables I to VI; (d) an isolated nucleic acid sequence that is hybridizable to any of the gene sequences according to (a), (b) or (c) under stringent hybridisation conditions; (e) a recombinant vector comprising a gene sequence or nucleic acid sequence according to any one of (a) to (d); (f) a host cell containing the vector according to (e); (g) a non-human animal having in its genome an introduced gene sequence or nucleic acid sequence or a removed or down-regulated gene sequence or nucleic acid sequence according to any one of (a) to (d); (h) an isolated polypeptide comprising an amino acid sequence at least 90% identical to an amino acid sequence encoded by a nucleotide sequence according to any one of (a) to (d), or a polypeptide variant thereof with sequential amino acid deletions from the C terminus and/or the N-terminus; (i) an isolated polypeptide encoded by a nucleotide sequence according to any one of (a) to (d); or (j) an isolated antibody that binds specifically to a polypeptide according to (h) or (i); in the screening of compounds for the treatment of pain, or for the diagnosis of pain.
 2. Use according to claim 1, wherein the isolated gene sequence is down-regulated both in response to streptozocin-induced diabetes and in response to surgical injury of a nerve leading to the spine.
 3. Use according to claim 1 wherein the isolated gene sequence encodes a kinase.
 4. Use according to claim 3, wherein the isolated gene sequence encodes an expression product or fragment thereof of pyruvate kinase, M1 and M2 subunits (M24359; X97047; X56494).
 5. Use according to claim 1, wherein the isolated gene sequence encodes an expression product or fragment thereof of a receptor.
 6. Use according to claim 5, wherein the isolated gene sequence encodes dopamine receptor D.sub.1 (158000).
 7. Use according to claim 1, wherein the isolated gene sequence encodes a transporter.
 8. Use according to claim 7, wherein the isolated gene sequence encodes differentiation-associated Na⁺-dependent inorganic phosphate cotransporter (AF271235) or putative vacuolar assembly protein VSP41 gene (U87309).
 9. Use according to claim 1, wherein the isolated gene sequence encodes a G-protein coupled receptor protein.
 10. Use according to claim 9, wherein the isolated gene sequence encodes Git1 (G-protein-coupled receptor kinase-interactor 1; GPCR kinase-associated ADP-ribosylation factor) (AF085693).
 11. Use according to claim 1, wherein the isolated gene sequence encodes a DNA-binding protein.
 12. Use according to claim 11, wherein the isolated gene sequence encodes putative histone H3.3A (X91866; M11354).
 13. Use according to claim 1, wherein the isolated gene sequence encodes a ligase.
 14. Use according to claim 13, wherein the isolated gene sequence encodes 3-Hydroxy 3-methylglutaryl coenzyme A synthase, cytosolic (X52625), acyl-CoA synthetase II, brain (D360666) farnesyl diphosphate synthase (M34477), bendless protein (AB032739; E12457), fatty acid synthase (X62888), glutamine synthetase (EC 6.3.1.2) (M91652), or putative seryl-tRNA synthetase (X91257).
 15. Use according to claim 1, wherein the isolated gene sequence encodes a lyase.
 16. Use according to claim 15, wherein the lyase is enolase, alpha alpha, non-neuronal (NNE) (X02610; X52379; M14328).
 17. Use according to claim 1, wherein the isolated gene sequence encodes an oxidoreductase.
 18. Use according to claim 17, wherein the isolated gene sequence encodes aldose reductase, lens (AREC 11.1.21) (X05884) cytochrome-c oxidase I, mitochondrial (S79304), lactate dehydrogenase-B (LDH-B) (U07181; X51905; Y00711), putative cytochrome c oxidase VIB (EC 1.9.3.1) (X13923), putative NADH: ubiquinone oxidoreductase PGIV subunit (AF044953), putative succinate dehydrogenase flavoprotein (AF095938; AF171022), putative ubiquinol-cytochrome-c reductase (EC 1.10.2.2) core protein II (J04973) or stearoyl-coA desaturase 2 (AB032243; M26270).
 19. Use according to claim 1, wherein the isolated sequence encodes a transferase.
 20. Use according to claim 19, wherein the isolated sequence encodes ribophorin I (X05300) or sulfotransferase-like protein (AF188699).
 21. Use according to claim 1, wherein the isolated sequence encodes a hydrolase.
 22. Use according to claim 21, wherein the isolated sequence encodes ATP synthase, H+, alpha subunit, mitochondrial (EC 3.6.1.34) (X56133), F1F0 ATPase delta subunit (U00926), putative dihydropyrimidinase related protein (D78013), heat shock protein 90 (S45392; M18186; M16660), or putative ribonuclease III (AF116910).
 23. Use according to claim 1, wherein the isolated sequence encodes myelin basic protein S (MBP S) (K00512), transferring (D38380), neurofilament, light molecular weight (NF-L) (AF031880), myelin-associayted glycoprotein (MAG) (M16800; M31811), NF-M middle molecular weight neurofilament protein (M18628) neuro-degeneration associated-protein 1 (D32249), S-100 protein β-subunit (X01090), microtubule-associated protein 1b (Map 1b) (X60370; L06237), putative cdc 37 homolog (D26564), putative ras-related protein Rab-5c (U11293), putative gelsolin (J04953), Cd81 antigen (target of antiproliferative antibody 1) (U19894; X59047; M33680), Mobp81 (Myelin-associated/Oligo-dendrocytic basic protein 81) (X87900), syntaxin binding protein n-sec1, sec1 homolog, A-internexin (M73049), putative β-sarcoglycan A3b (AB024921), CGI-78 protein (AF151835), KIAA0143 (D63477), septin 2 (D50918), Nucleobindin (Z36277), myelin protein SR13 (M69139; S55427), B-Actin, cytoplasmic (V01217; X03672), ly6/neurotoxin (Lynx1) homolog (AD141377), astrocytic phosphoprotein; PFA 15 gene (AJ243949; X86694), PLIC-1 (AF177345), Nfx1 (tip associating protein (TAP) gene) (AF093139; AF093140), A-Crystallin B (U04320; M73741; M28638), heat shock-like protein 70 kD (X70065; U73744; Y00371), tau microtubule-associated protein (X79321), myelin, Scgwann cell, Perioheral (P-0) (K03242), B-Tubulin class 1 (ABO11679; X04663; AF14139), putative long-chain polyunsaturated fatty acid elongation enzyme (Helo1) or peptidylglycine alpha-amidating monooxygenase
 1. 24. A non-human animal having in its genome an introduced gene sequence or a removed or down-regulated gene sequence, said sequence being down-regulated in the spinal cord of a mammal in response to first and second models of neuropathic or central sensitisation pain.
 25. A non-human animal according to claim 24, wherein said gene sequence becomes down regulated both in response to streptozocin induced diabetes and in response to chronic constriction injury.
 26. A non-human animal according to claim 24, wherein the introduced gene sequence encodes a kinase, an expression product or fragment thereof, a transporter, a G-protein coupled receptor, a DNA bidning protein, a ligase, a lyase, an oxidoreductase, transferase, or a hydrolase.
 27. A non-human animal according to claim 24 which is C. elegans.
 28. A kit comprising; (a) affinity peptide and/or ligand and/or substrate for an expression product of a gene sequence that is down-regulated in the spinal cord of a mammal in response to a mechanistically distinct first and second models of neuropathic or central sensitization pain; and (b) a defined quantity of an expression product of a gene sequence that is down-regulated in the spinal cord of a mammal both in response to first and second models of neuropathic or central sensitization pain, for simultaneous, separate or sequential use in detecting and/or quantifying down-regulation of a gene sequence in the spinal cord of a mammal in response to first and second models of neuropathic or central sensitization pain.
 29. A kit according to claim 28, wherein the gene sequence encodes a kinase, an expression product or fragment thereof, a transporter, a G-protein coupled receptor, a DNA bidning protein, a ligase, a lyase, an oxidoreductase, transferase, or a hydrolase.
 30. A kit comprising: (a) nucleic acid sequences capable of hybridization to a nucleic acid sequence that is down-regulated in the spinal cord of a mammal in response to first and second models of neuropathic or central sensitization pain; and (b) a defined quantity of one or more nucleic acid sequences capable of hybridization to a nucleic acid sequence that is down-regulated in the spinal cord of a mammal in response to first and second models of neuropathic or central sensitization pain, for simultaneous, separate or sequential use in detecting and/or quantifying down-regulation of a gene sequence in the spinal cord of a mammal in response to first and second models of neuropathic or central sensitization pain.
 31. The kit of claim 30, wherein the gene sequence encodes a kinase, an expression product or fragment thereof, a transporter, a G-protein coupled receptor, a DNA bidning protein, a ligase, a lyase, an oxidoreductase, transferase, or a hydrolase.
 32. A compound that modulates the action of an expression product of a gene sequence that is down-regulated in the spinal cord of a mammal in response to first and second models of neuropathic or central sensitization pain.
 33. A compound according to claim 32 wherein the gene sequence is listed in Tables I to VI.
 34. A compound according to claim 32 wherein the nucleotide sequence encodes a kinase, an expression product or fragment thereof, a transporter, a G-protein coupled receptor, a DNA bidning protein, a ligase, a lyase, an oxidoreductase, transferase, or a hydrolase.
 35. A compound according to claim 32 for use as a medicament.
 36. A compound according to claim 32 for the treatment or diagnosis of pain.
 37. A pharmaceutical composition comprising a compound according to claim 32 and a pharmaceutically acceptable carrier or diluent.
 38. Use of a compound according to claim 32 in the manufacture of a medicament for the treatment or diagnosis of pain.
 39. Use of a compound according to any one of claims 32 in the manufacture of a medicament for the treatment or diagnosis of chronic pain.
 40. A method of treatment of pain, which comprises administering to a patient an effective amount of a compound according to claim
 32. protein coupled receptor, a DNA bidning protein, a ligase, a lyase, an oxidoreuctase, transferase, or a hydrolase. 